| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37451-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06478-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26422-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26414-50ul | |||
![Western Blot: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis using FZD5 mouse mAb against A549 (1), and PC-3 (2) cell lysate.](http://images.novusbio.com/fullsize/Frizzled-5-Antibody-2D12-Western-Blot-NBP2-37451-img0003.jpg "Western Blot: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis using FZD5 mouse mAb against A549 (1), and PC-3 (2) cell lysate.")
![Flow Cytometry: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis of HepG2 cells using FZD5 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Frizzled-5-Antibody-2D12-Flow-Cytometry-NBP2-37451-img0004.jpg)
![Western Blot: Frizzled-5 Antibody (2D12) [NBP2-37451] - Analysis using FZD5 mAb against human FZD5 recombinant protein. (Expected MW is 32.5 kDa).](http://images.novusbio.com/fullsize/Frizzled-5-Antibody-2D12-Western-Blot-NBP2-37451-img0002.jpg)
![ELISA: Frizzled-5 Antibody (2D12) [NBP2-37451] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Frizzled-5-Antibody-2D12-ELISA-NBP2-37451-img0001.jpg)
![Western Blot: Wnt-8a Antibody (PSH0-39) [NBP3-33103] - Western blot analysis of Wnt-8a on different lysates with Rabbit anti-Wnt-8a antibody (NBP3-33103) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 ug/Lane) Lane 2: HepG2 cell lysate (30 ug/Lane) Lane 3: SW480 cell lysate (30 ug/Lane) Lane 4: Jurkat cell lysate (30 ug/Lane) Lane 5: Raji cell lysate (30 ug/Lane) Lane 6: NIH/3T3 cell lysate (30 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Mouse embryo tissue lysate (40 ug/Lane) Lane 9: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 38.8 kDa Observed band size: 55 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33103) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33103_rabbit-wnt-8a-mab-psh0-39-28820241419154.jpg "Western Blot: Wnt-8a Antibody (PSH0-39) [NBP3-33103] - Western blot analysis of Wnt-8a on different lysates with Rabbit anti-Wnt-8a antibody (NBP3-33103) at 1/1,000 dilution. Lane 1: HeLa cell lysate (30 ug/Lane) Lane 2: HepG2 cell lysate (30 ug/Lane) Lane 3: SW480 cell lysate (30 ug/Lane) Lane 4: Jurkat cell lysate (30 ug/Lane) Lane 5: Raji cell lysate (30 ug/Lane) Lane 6: NIH/3T3 cell lysate (30 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Mouse embryo tissue lysate (40 ug/Lane) Lane 9: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 38.8 kDa Observed band size: 55 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33103) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Wnt-8a Antibody (PSH0-39) [NBP3-33103] - Immunocytochemistry analysis of NIH/3T3 cells labeling Wnt-8a with Rabbit anti-Wnt-8a antibody (NBP3-33103) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Wnt-8a antibody (NBP3-33103) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33103_rabbit-wnt-8a-mab-psh0-39-29202410484880.jpg)
![Flow Cytometry: Wnt-8a Antibody (PSH0-39) [NBP3-33103] - Flow cytometric analysis of HeLa cells labeling Wnt-8a. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33103, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33103_rabbit-wnt-8a-mab-psh0-39-392024884647.jpg)
![Western Blot: beta-Catenin Antibody (A6-F8-R) [NBP3-32079] - Western blot analysis of beta-Catenin on different lysates with Mouse anti-beta-Catenin antibody (NBP3-32079) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: A431 cell lysate Lane 3: NCCIT cell lysate Lane 4: SW480 cell lysate Lane 5: HT-29 cell lysate Lane 6: HCT 116 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32079) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32079_mouse-beta-catenin-mab-a6-f8-r-278202415212464.jpg "Western Blot: beta-Catenin Antibody (A6-F8-R) [NBP3-32079] - Western blot analysis of beta-Catenin on different lysates with Mouse anti-beta-Catenin antibody (NBP3-32079) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: A431 cell lysate Lane 3: NCCIT cell lysate Lane 4: SW480 cell lysate Lane 5: HT-29 cell lysate Lane 6: HCT 116 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32079) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (A6-F8-R) [NBP3-32079] - Immunocytochemistry analysis of MCF7 cells labeling beta-Catenin with Mouse anti-beta-Catenin antibody (NBP3-32079) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta-Catenin antibody (NBP3-32079) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32079_mouse-beta-catenin-mab-a6-f8-r-29202410334026.jpg)
![Flow Cytometry: beta-Catenin Antibody (A6-F8-R) [NBP3-32079] - Flow cytometric analysis of MCF7 cells labeling beta-Catenin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32079, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 594 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32079_mouse-beta-catenin-mab-a6-f8-r-392024884635.jpg)
![Western Blot: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32847) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Human brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Rat brain tissue lysate (40 ug/Lane) Lane 11: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32847) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32847_rabbit-ppp2r5d-mab-psh01-22-288202412461614.jpg "Western Blot: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Western blot analysis of PPP2R5D on different lysates with Rabbit anti-PPP2R5D antibody (NBP3-32847) at 1/1,000 dilution. Lane 1: A431 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: 293T cell lysate (20 ug/Lane) Lane 6: Jurkat cell lysate (20 ug/Lane) Lane 7: NIH/3T3 cell lysate (20 ug/Lane) Lane 8: Human brain tissue lysate (40 ug/Lane) Lane 9: Mouse brain tissue lysate (40 ug/Lane) Lane 10: Rat brain tissue lysate (40 ug/Lane) Lane 11: Rat testis tissue lysate (40 ug/Lane) Predicted band size: 70 kDa Observed band size: 56~70 kDa Exposure time: 2 minutes 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32847) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PPP2R5D antibody (NBP3-32847) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32847) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32847_rabbit-ppp2r5d-mab-psh01-22-298202414304124.jpg)
![Flow Cytometry: PPP2R5D Antibody (PSH01-22) [NBP3-32847] - Flow cytometric analysis of A431 cells labeling PPP2R5D. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32847, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32847_rabbit-ppp2r5d-mab-psh01-22-392024884664.jpg)
![Western Blot: PPP2R5E Antibody [NBP3-06478] - Lane A: HeLa Whole Cell LysateLane B: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:53 kDa](http://images.novusbio.com/fullsize/PPP2R5E-Antibody-Western-Blot-NBP3-06478-img0001.jpg "Western Blot: PPP2R5E Antibody [NBP3-06478] - Lane A: HeLa Whole Cell LysateLane B: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:55 kDaObserved band size:53 kDa")
![Immunohistochemistry-Paraffin: PPP2R5E Antibody [NBP3-06478] - Staining of human PPP2R5E in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/PPP2R5E-Antibody-Immunohistochemistry-Paraffin-NBP3-06478-img0002.jpg)
![Immunoprecipitation: PPP2R5E Antibody [NBP3-06478] - Lane A:0.5 mg 293 Whole Cell Lysate4 uL anti-PPP2R5E rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PPP2R5E rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 55 kDaObserved band size :53 kDa](http://images.novusbio.com/fullsize/PPP2R5E-Antibody-Immunoprecipitation-NBP3-06478-img0003.jpg)
![Western Blot: GSK-3 beta Antibody (M131) [NBP3-23366] - Western blot analysis of a human recombinant GSK-3 beta N-terminal fragment (lanes 1 & 2) and endogenous GSK-3 beta expressed in mouse brain (lanes 3 & 4). The blot was probed with mouse monoclonal anti-GSK-3 beta at 1:500 (lanes 1 & 3) and 1:2000 (lanes 2 & 4).](http://images.novusbio.com/fullsize/nbp3-23366_mouse-gsk-3-beta-mab-m131-1992023199282.jpg "Western Blot: GSK-3 beta Antibody (M131) [NBP3-23366] - Western blot analysis of a human recombinant GSK-3 beta N-terminal fragment (lanes 1 & 2) and endogenous GSK-3 beta expressed in mouse brain (lanes 3 & 4). The blot was probed with mouse monoclonal anti-GSK-3 beta at 1:500 (lanes 1 & 3) and 1:2000 (lanes 2 & 4).")
![Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (M131) [NBP3-23366] - Immunocytochemical labeling of GSK-3 beta in aldehyde-fixed and NP-40 permeabilized human NCI-H1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-GSK-3 beta (NBP3-23366) antibody. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23366_mouse-gsk-3-beta-mab-m131-19920231252364.jpg)
![Western Blot: PRKX Antibody [NBP1-33469] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PRKX-Antibody-Western-Blot-NBP1-33469-img0005.jpg "Western Blot: PRKX Antibody [NBP1-33469] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: PRKX Antibody [NBP1-33469] - Paraffin-embedded H3347 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PRKX-Antibody-Immunohistochemistry-Paraffin-NBP1-33469-img0002.jpg)
![Western Blot: PRKX Antibody [NBP1-33469] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PRKX-Antibody-Western-Blot-NBP1-33469-img0004.jpg)
![Immunohistochemistry: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Image of GSK-3 beta [p Ser9] Antibody (3A6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-26220241525330.jpg "Immunohistochemistry: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Image of GSK-3 beta [p Ser9] Antibody (3A6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate (treated with Calyculin A or not). All lanes: GSK-3 beta [p Ser9] Antibody at 0.77ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46 KDa Observed band size: 46 KDa](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-28220241136300.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Staining of Hela cells(treated with 50mM Calyculin A for 30min) with GSK-3 beta [p Ser9] Antibody (3A6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-28220241312216.jpg)
![Immunohistochemistry: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Image of c-jun [p Ser63] Antibody (4A11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26414_rabbit-c-jun-p-ser-63-mab-4a11-2722024850463.jpg "Immunohistochemistry: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Image of c-jun [p Ser63] Antibody (4A11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Positive Western Blot detected in: Hela whole cell lysate, A549 whole cell lysate (treated with Calyculin A or EGF). All lanes: c-jun [p Ser63] Antibody at 0.95 ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48 KDa Observed band size: 48 KDa](http://images.novusbio.com/fullsize/nbp3-26414_rabbit-c-jun-p-ser-63-mab-4a11-282202411472127.jpg)
![Immunocytochemistry/Immunofluorescence: c-jun [p Ser63] Antibody (4A11) [NBP3-26414] - Staining of A549 cells with c-jun [p Ser63] Antibody (4A11) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26414_rabbit-c-jun-p-ser-63-mab-4a11-28220241312248.jpg)
![Western Blot: CKII alpha Antibody [NBP2-15909] - Sample (30 ug of whole cell lysate) B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CKII-alpha-Antibody-Western-Blot-NBP2-15909-img0002.jpg "Western Blot: CKII alpha Antibody [NBP2-15909] - Sample (30 ug of whole cell lysate) B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: CKII alpha Antibody [NBP2-15909] - Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CKII-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-15909-img0003.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody [NBP2-15909] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (NBP2-15909) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15909_rabbit-polyclonal-casein-kinase-2-alpha-antibody-91020242014672.jpg)
![Western Blot: ROCK2 Antibody [NB100-593] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROCK2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NB100-593-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: ROCK2 Antibody [NB100-593] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROCK2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ROCK2 Antibody [NB100-593] - Detection of Human Rho Kinase Alpha on HeLa whole cell lysate using NB100-593. NB100-593 and NB100-592 were used at 5 ug/mg lysate to immunoprecipitate Rho Kinase Alpha.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NB100-593-img0002.jpg)