| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15589-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15485-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18335-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52450-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54541-100ug | |||
![Western Blot: Smad2/3 Antibody [NBP3-15589] - Western blot analysis of extracts of various cell lines, using Smad2/3 antibody (NBP3-15589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Smad2-3-Antibody-Western-Blot-NBP3-15589-img0001.jpg "Western Blot: Smad2/3 Antibody [NBP3-15589] - Western blot analysis of extracts of various cell lines, using Smad2/3 antibody (NBP3-15589) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.")
![Immunocytochemistry/ Immunofluorescence: Smad2/3 Antibody - Azide and BSA Free [Smad2/3] - Immunofluorescence analysis of Hep G2 cells using Smad2/3 Rabbit pAb at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15589_rabbit-smad2-3-pab-52202517361286.jpg)
![Immunohistochemistry: Smad2/3 Antibody - Azide and BSA Free [Smad2/3] - Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using Smad2/3 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15589_rabbit-smad2-3-pab-52202517311036.jpg)
![Immunohistochemistry: Smad2/3 Antibody - Azide and BSA Free [Smad2/3] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Smad2/3 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15589_rabbit-smad2-3-pab-52202517303110.jpg)
![Immunohistochemistry: Smad2/3 Antibody - Azide and BSA Free [Smad2/3] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Smad2/3 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15589_rabbit-smad2-3-pab-52202517352770.jpg)
![Immunocytochemistry/Immunofluorescence: RhoA Antibody [NBP3-15485] - Immunofluorescence analysis of U2OS cells using RhoA antibody (NBP3-15485) at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-15485-img0003.jpg "Immunocytochemistry/Immunofluorescence: RhoA Antibody [NBP3-15485] - Immunofluorescence analysis of U2OS cells using RhoA antibody (NBP3-15485) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: RhoA Antibody [NBP3-15485] - Human colon carcinoma using [KO Validated] RhoA Rabbit pAb (NBP3-15485) at dilution of 1:350 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/RhoA-Antibody-Immunohistochemistry-Paraffin-NBP3-15485-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: RhoA Antibody [NBP3-15485] - Western blot analysis of extracts from normal (control) and RhoA knockout (KO) HeLa cells, using RhoA antibody (NBP3-15485) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RhoA-Antibody-Knockout-Validated-NBP3-15485-img0001.jpg)
![Western Blot: Frizzled-6 Antibody [NBP3-18335] - WB Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysate. Antibodies: Frizzled-6 NBP3-18335, 2 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.](http://images.novusbio.com/fullsize/Frizzled-6-Antibody-Western-Blot-NBP3-18335-img0001.jpg "Western Blot: Frizzled-6 Antibody [NBP3-18335] - WB Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysate. Antibodies: Frizzled-6 NBP3-18335, 2 ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.")
![Immunocytochemistry/ Immunofluorescence: Frizzled-6 Antibody - BSA Free [NBP3-18335] - Validation of FZD6 in Mouse Lung.Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung labeling FZD6 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp3-18335_rabbit-frizzled-6-pab-2711202419293843.jpg)
![Immunocytochemistry/ Immunofluorescence: Frizzled-6 Antibody - BSA Free [NBP3-18335] - Validation of FZD6 in Rat Lung. Immunofluorescent analysis of 4% paraformaldehyde-fixed rat lung labeling FZD6 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp3-18335_rabbit-frizzled-6-pab-271120241958444.jpg)
![Immunocytochemistry/ Immunofluorescence: Frizzled-6 Antibody - BSA Free [NBP3-18335] - Validation of FZD6 in Daudi Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Daudi cells labeling FZD6 with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).](http://images.novusbio.com/fullsize/nbp3-18335_rabbit-frizzled-6-pab-271120241956217.jpg)
![Western Blot: Casein Kinase 2 beta Antibody (1H8A5) [NBP2-52450] - Analysis using CSNK2B mouse mAb against Hela (1), Jurkat (2), C6 (3), MCF-7 (4), SK-N-SH (5), NTERA-2 (6) cell lysate.](http://images.novusbio.com/fullsize/Casein-Kinase-2-beta-Antibody-1H8A5-Western-Blot-NBP2-52450-img0005.jpg "Western Blot: Casein Kinase 2 beta Antibody (1H8A5) [NBP2-52450] - Analysis using CSNK2B mouse mAb against Hela (1), Jurkat (2), C6 (3), MCF-7 (4), SK-N-SH (5), NTERA-2 (6) cell lysate.")
![Flow Cytometry: Casein Kinase 2 beta Antibody (1H8A5) [NBP2-52450] - Flow cytometric analysis of Hela cells using CSNK2B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Casein-Kinase-2-beta-Antibody-1H8A5-Flow-Cytometry-NBP2-52450-img0001.jpg)
![Western Blot: Casein Kinase 2 beta Antibody (1H8A5) [NBP2-52450] - Analysis using CSNK2B mAb against HEK293 (1) and CSNK2B (AA: FULL(1-215))-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Casein-Kinase-2-beta-Antibody-1H8A5-Western-Blot-NBP2-52450-img0003.jpg)
![Western Blot: Casein Kinase 2 beta Antibody (1H8A5) [NBP2-52450] - Analysis using CSNK2B mAb against human CSNK2B (AA: FULL(1-215)) recombinant protein. (Expected MW is 35 kDa)](http://images.novusbio.com/fullsize/Casein-Kinase-2-beta-Antibody-1H8A5-Western-Blot-NBP2-52450-img0002.jpg)
![ELISA: Casein Kinase 2 beta Antibody (1H8A5) [NBP2-52450] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Casein-Kinase-2-beta-Antibody-1H8A5-ELISA-NBP2-52450-img0004.jpg)
![Western Blot: ROCK2 Antibody (JE31-36) [NBP3-32914] - Western blot analysis of ROCK2 on different lysates with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 161 kDa Observed band size: 150 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32914) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32914_rabbit-rock2-mab-je31-36-288202412461668.jpg "Western Blot: ROCK2 Antibody (JE31-36) [NBP3-32914] - Western blot analysis of ROCK2 on different lysates with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: C2C12 cell lysate Lane 4: C6 cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 161 kDa Observed band size: 150 kDa Exposure time: 59 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32914) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ROCK2 Antibody (JE31-36) [NBP3-32914] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32914) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32914_rabbit-rock2-mab-je31-36-298202414262644.jpg)
![Immunocytochemistry/ Immunofluorescence: ROCK2 Antibody (JE31-36) [NBP3-32914] - Immunocytochemistry analysis of HeLa cells labeling ROCK2 with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ROCK2 antibody (NBP3-32914) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32914_rabbit-rock2-mab-je31-36-29202410334061.jpg)
![Western Blot: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Analysis of CaMKII alpha on rat brain cells lysates using anti-CaMKII alpha antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-JM11-07-Western-Blot-NBP2-67207-img0001.jpg "Western Blot: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Analysis of CaMKII alpha on rat brain cells lysates using anti-CaMKII alpha antibody at 1/500 dilution.")
![Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-CaMKII alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-JM11-07-Immunohistochemistry-Paraffin-NBP2-67207-img0004.jpg)
![Flow Cytometry: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Flow cytometric analysis of CaM Kinase II alpha was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-JM11-07-Flow-Cytometry-NBP2-67207-img0002.jpg)
![Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody (JM11-07) [NBP2-67207] - Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-CaM Kinase II alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CaM-Kinase-II-alpha-Antibody-JM11-07-Immunohistochemistry-Paraffin-NBP2-67207-img0003.jpg)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5097] - Anti-FZD9 / Frizzled 9 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5097-img0005.jpg "Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5097] - Anti-FZD9 / Frizzled 9 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5097] - Analysis of anti-FZD9 / Frizzled 9 antibody with brains, neurons and glia at 14 ug/ ml.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5097-img0002.jpg)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5097] - Analysis of anti-FZD9 / Frizzled 9 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5097-img0003.jpg)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5097] - Anti-FZD9 / Frizzled 9 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5097-img0004.jpg)
![Immunohistochemistry-Paraffin: Frizzled-9 Antibody [NLS5097] - Anti-FZD9 / Frizzled 9 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Frizzled-9-Antibody-Immunohistochemistry-Paraffin-NLS5097-img0006.jpg)
![Western Blot: LEF1 Antibody (JE52-28) [NBP3-32527] - Western blot analysis of LEF1 on different lysates with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (negative) (20 ug/Lane) Lane 3: Mouse thymus tissue lysate (40 ug/Lane) Lane 4: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 44 kDa Observed band size: 35-55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32527) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-278202415212459.jpg "Western Blot: LEF1 Antibody (JE52-28) [NBP3-32527] - Western blot analysis of LEF1 on different lysates with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. Lane 1: Jurkat cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (negative) (20 ug/Lane) Lane 3: Mouse thymus tissue lysate (40 ug/Lane) Lane 4: Rat thymus tissue lysate (40 ug/Lane) Predicted band size: 44 kDa Observed band size: 35-55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32527) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LEF1 Antibody (JE52-28) [NBP3-32527] - Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32527) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-298202414333615.jpg)
![Immunocytochemistry/ Immunofluorescence: LEF1 Antibody (JE52-28) [NBP3-32527] - Immunocytochemistry analysis of Jurkat (positive) and HeLa (negative) labeling LEF1 with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LEF1 antibody (NBP3-32527) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32527_rabbit-lef1-mab-je52-28-2920241023422.jpg)
![Western Blot: JNK1 Antibody (JE63-04) [NBP3-32487] - Western blot analysis of JNK1 on different lysates with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 44/48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32487) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-27820241631699.jpg "Western Blot: JNK1 Antibody (JE63-04) [NBP3-32487] - Western blot analysis of JNK1 on different lysates with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: Jurkat cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: C6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 44/48 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32487) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: JNK1 Antibody (JE63-04) [NBP3-32487] - Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32487) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-29820241435284.jpg)
![Immunocytochemistry/ Immunofluorescence: JNK1 Antibody (JE63-04) [NBP3-32487] - Immunocytochemistry analysis of HEK-293 cells labeling JNK1 with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-JNK1 antibody (NBP3-32487) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32487_rabbit-jnk1-mab-je63-04-2920241192320.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1508) [NBP2-53365] - Formalin-fixed, paraffin-embedded Rat Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1508).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1508-Immunohistochemistry-Paraffin-NBP2-53365-img0004.jpg "Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1508) [NBP2-53365] - Formalin-fixed, paraffin-embedded Rat Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1508).")
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1508) [NBP2-53365] - Formalin-fixed, paraffin-embedded human Pancreas stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1508).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1508-Immunohistochemistry-Paraffin-NBP2-53365-img0001.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1508) [NBP2-53365] - Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1508).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1508-Immunohistochemistry-Paraffin-NBP2-53365-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1508) [NBP2-53365] - Formalin-fixed, paraffin-embedded Mouse Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1508).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1508-Immunohistochemistry-Paraffin-NBP2-53365-img0003.jpg)
![Western Blot: PPP2CB Antibody [NBP1-32069] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A beta antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PPP2CB-Antibody-Western-Blot-NBP1-32069-img0006.jpg "Western Blot: PPP2CB Antibody [NBP1-32069] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A beta antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: PPP2CB Antibody [NBP1-32069] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PPP2CB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32069-img0002.jpg)
![Western Blot: PPP2CB Antibody [NBP1-32069] - Sample(30 ug of whole cell lysate) A:293T B:A431 12% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/PPP2CB-Antibody-Western-Blot-NBP1-32069-img0004.jpg)
![Western Blot: PPP2CB Antibody [NBP1-32069] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PPP2CB-Antibody-Western-Blot-NBP1-32069-img0005.jpg)
![Western Blot: Smad3 [p Thr179] Antibody [NBP2-19126] - NMuMG mouse mammary epithelial cells were probed for the activation of Smad3 by detecting phosphorylation of threonine 179. The cells were either untreated or treated with TGF-beta, transferred to membranes and probed with Anti-SMAD3 pT179 Antibody. The antibody detects only Smad3 in stimulated cells suggesting detection of phosphorylated SMAD3 at T179.](http://images.novusbio.com/fullsize/Smad3-[p-Thr179]-Antibody-Western-Blot-NBP2-19126-img0001.jpg "Western Blot: Smad3 [p Thr179] Antibody [NBP2-19126] - NMuMG mouse mammary epithelial cells were probed for the activation of Smad3 by detecting phosphorylation of threonine 179. The cells were either untreated or treated with TGF-beta, transferred to membranes and probed with Anti-SMAD3 pT179 Antibody. The antibody detects only Smad3 in stimulated cells suggesting detection of phosphorylated SMAD3 at T179.")
![ELISA: Smad3 [p Thr179] Antibody [NBP2-19126] - The SMAD pathway follows the canonical TGF- Beta signaling pathway. TGF- Beta dimers bind to a receptor thereby activating the pathway. The type I receptor then recruits and phosphorylates a receptor regulated SMAD.i.e. SMAD2 or SMAD3. The R-SMAD then binds to the common SMAD i.e. SMAD4, and forms a heterodimeric complex. This complex then enters the cell nucleus and acts as a transcription factor.](http://images.novusbio.com/fullsize/Smad3 [p Thr179] Antibody-ELISA-NBP2-19126-img0003.jpg)