| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97476-0.2mg | |||
| BSA Free | NBP1-97476-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54552-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37279-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59692-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88023-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-54318-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26497-50ul | |||
![Western Blot: Calnexin Antibody [NBP1-97476] - Lane 1: MWM, Lane 2: Vero, Lane 3: 3T3, Lane 4: PC-12, Lane 5: HeLa.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Western-Blot-NBP1-97476-img0002.jpg "Western Blot: Calnexin Antibody [NBP1-97476] - Lane 1: MWM, Lane 2: Vero, Lane 3: 3T3, Lane 4: PC-12, Lane 5: HeLa.")
![Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-97476] - Analysis of human placenta tissue stained with Calnexin, pAb at 5ug/ml.](http://images.novusbio.com/fullsize/Calnexin-Antibody-Immunohistochemistry-Paraffin-NBP1-97476-img0001.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-42848] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hspa5 antibody diluted at a dilution of 1:10000.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-42848-img0009.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP2-42848] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hspa5 antibody diluted at a dilution of 1:10000.")
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-42848] - Paraffin-embedded rat brain. Hspa5 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-42848-img0008.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-42848] - Analysis of 30 ug whole cell lysate. A: NIH-3T-3 B: JC C: BCL-1 D: C2C12 E: Raw264.7 7.5% SDS PAGE diluted at 1:10000..](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-42848-img0003.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP2-42848] - Analysis of A. 50 ug Rat heart lysate/extract B. 50 ug Rat Lung lysate/extract C. 50 ug Rat Liver lysate/extract D. 50 ug Rat kidney lysate/extract 7.5 % SDS-PAGE Hspa5 antibody dilution: 1:10000](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP2-42848-img0004.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-42848] - Analysis of gastric carcinoma. Hspa5 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-42848-img0001.jpg)
![Immunohistochemistry-Paraffin: GRP78/HSPA5 Antibody [NBP2-42848] - Paraffin-embedded mouse liver. Hspa5 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunohistochemistry-Paraffin-NBP2-42848-img0007.jpg)
![Western Blot: Calnexin Antibody (CANX/1541) [NBP2-53352] - Western Blot Analysis of HeLa and MCF-7 cell lysate using Calnexin Antibody (CANX/1541).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1541-Western-Blot-NBP2-53352-img0007.jpg "Western Blot: Calnexin Antibody (CANX/1541) [NBP2-53352] - Western Blot Analysis of HeLa and MCF-7 cell lysate using Calnexin Antibody (CANX/1541).")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody (CANX/1541) [NBP2-53352] - Confocal immunofluorescence image of HeLa cells using Calnexin Antibody (CANX/1541), labeled in Green. Red Dot is used to label the nuclei Red.](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1541-Immunocytochemistry-Immunofluorescence-NBP2-53352-img0005.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (CANX/1541) [NBP2-53352] - Formalin-fixed, paraffin-embedded human Small Intestinal Carcinoma stained with Calnexin Monoclonal Antibody (CANX/1541).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1541-Immunohistochemistry-Paraffin-NBP2-53352-img0003.jpg)
![Western Blot: Calnexin Antibody (CANX/1541) [NBP2-53352] - Western Blot Analysis of Human Kidney lysate using Calnexin Antibody (CANX/1541).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1541-Western-Blot-NBP2-53352-img0006.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (CANX/1541) [NBP2-53352] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Calnexin Monoclonal Antibody (CANX/1541).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1541-Immunohistochemistry-Paraffin-NBP2-53352-img0001.jpg)
![Immunohistochemistry-Paraffin: Calnexin Antibody (CANX/1541) [NBP2-53352] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Calnexin Monoclonal Antibody (CANX/1541).](http://images.novusbio.com/fullsize/Calnexin-Antibody-CANX-1541-Immunohistochemistry-Paraffin-NBP2-53352-img0002.jpg)
![Western Blot: eIF2A Antibody (3A7A8) [NBP2-37279] - Western blot analysis using EIF2A mouse mAb against MCF-7 (1), PC-12 (2), HepG2 (3), Hela (4), Cos7 (5), K562 (6), Jurkat (7), A431 (8) and NIH/3T3 (9) cell lysate.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Western-Blot-NBP2-37279-img0004.jpg "Western Blot: eIF2A Antibody (3A7A8) [NBP2-37279] - Western blot analysis using EIF2A mouse mAb against MCF-7 (1), PC-12 (2), HepG2 (3), Hela (4), Cos7 (5), K562 (6), Jurkat (7), A431 (8) and NIH/3T3 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: eIF2A Antibody (3A7A8) [NBP2-37279] - Immunofluorescence analysis of HepG2 cells using EIF2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Immunocytochemistry-Immunofluorescence-NBP2-37279-img0005.jpg)
![Immunohistochemistry: eIF2A Antibody (3A7A8) [NBP2-37279] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EIF2A mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Immunohistochemistry-NBP2-37279-img0007.jpg)
![Flow Cytometry: eIF2A Antibody (3A7A8) [NBP2-37279] - Flow cytometric analysis of HepG2 cells using EIF2A mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Flow-Cytometry-NBP2-37279-img0006.jpg)
![Western Blot: eIF2A Antibody (3A7A8) [NBP2-37279] - Western blot analysis using EIF2A mAb against human EIF2A (AA: 448-576) recombinant protein. (Expected MW is 40.3 kDa)](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Western-Blot-NBP2-37279-img0002.jpg)
![Western Blot: eIF2A Antibody (3A7A8) [NBP2-37279] - Western blot analysis using EIF2A mAb against HEK293 (1) and EIF2A (AA: 448-576)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-Western-Blot-NBP2-37279-img0003.jpg)
![ELISA: eIF2A Antibody (3A7A8) [NBP2-37279] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/eIF2A-Antibody-3A7A8-ELISA-NBP2-37279-img0001.jpg)
![Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Western-Blot-NBP2-66856-img0007.jpg "Western Blot: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of DDIT3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Lane 1: LOVO cell lysate Lane 2: PC-12 cell lysate")
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded mouse pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0006.jpg)
![Flow Cytometry: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of Hela cells with DDIT3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Flow-Cytometry-NBP2-66856-img0001.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0002.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human gastric carcinoma tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0003.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded human pancreas tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0004.jpg)
![Immunohistochemistry-Paraffin: GADD153/CHOP Antibody (JM10-31) [NBP2-66856] - Analysis of paraffin-embedded mouse brain tissue using anti-DDIT3 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GADD153-CHOP-Antibody-JM10-31-Immunohistochemistry-Paraffin-NBP2-66856-img0005.jpg)
![Western Blot: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 . Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-3C5-1A4-Western-Blot-NBP2-59692-img0002.jpg "Western Blot: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Analysis of Human, Mouse, Rat NIH3T3, Rat Brain, and HEK-293 cell lysates showing detection of ~78 kDa GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 3C5-1A4 . Lane 1: MW ladder. Lane 2: Mouse NIH3T3. Lane 3: Rat Brain. Lane 4: Human HEK-293. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~78 kDa.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (3C5-1A4) [NBP2-59692] - Mouse fibroblast cell line (NIH 3T3). Fixed in 4% Formaldehyde for 15 min at RT. Primary antibody at 1:100 for 60 min at RT. Secondary antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstaied with DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-3C5-1A4-Immunocytochemistry-Immunofluorescence-NBP2-59692-img0003.jpg)
![Western Blot: XBP1 Antibody [NBP2-20917] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP2-20917-img0002.jpg "Western Blot: XBP1 Antibody [NBP2-20917] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody")
![Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with XBP1 antibody [N1C3] (NBP2-20917) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20917_rabbit-polyclonal-xbp1-antibody-western-blot-2611202518211016.jpg)
![Immunocytochemistry/Immunofluorescence: Derlin 1 Antibody [NBP1-88023] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88023-img0009.jpg "Immunocytochemistry/Immunofluorescence: Derlin 1 Antibody [NBP1-88023] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.")
![Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP1-88023] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88023-img0013.jpg)
![Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP1-88023] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88023-img0010.jpg)
![Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP1-88023] - Staining of human lung shows moderate to strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88023-img0011.jpg)
![Immunohistochemistry-Paraffin: Derlin 1 Antibody [NBP1-88023] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in lymphoid cells and in glandular cells.](http://images.novusbio.com/fullsize/Derlin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-88023-img0012.jpg)
![Western Blot: SKIV2L Antibody [NBP2-47274] - Analysis in control (vector only transfected HEK293T lysate) and SKIV2L over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/SKIV2L-Antibody-Western-Blot-NBP2-47274-img0006.jpg "Western Blot: SKIV2L Antibody [NBP2-47274] - Analysis in control (vector only transfected HEK293T lysate) and SKIV2L over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: SKIV2L Antibody [NBP2-47274] - Staining of human cell line HeLa shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/SKIV2L-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47274-img0005.jpg)
![Immunohistochemistry-Paraffin: SKIV2L Antibody [NBP2-47274] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/SKIV2L-Antibody-Immunohistochemistry-Paraffin-NBP2-47274-img0004.jpg)
![Western Blot: GRP78/HSPA5 Antibody [NBP1-54318] - Western blot analysis of Human Glucose deprived glia cell lysates showing detection of GRP78/HSPA5 protein using Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318). Primary Antibody: Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318) at 1:1000.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Western-Blot-NBP1-54318-img0008.jpg "Western Blot: GRP78/HSPA5 Antibody [NBP1-54318] - Western blot analysis of Human Glucose deprived glia cell lysates showing detection of GRP78/HSPA5 protein using Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318). Primary Antibody: Rabbit Anti-GRP78/HSPA5 Polyclonal Antibody (NBP1-54318) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody [NBP1-54318] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-GRP78 (Bip) Polyclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Nucleus. Magnification: 100x. Heat Shocked at 42C for 1h.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54318-img0006.jpg)
![Immunoprecipitation: ATF4 Antibody (9E1) [NBP3-26497] - Immunoprecipitating ATF4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26497 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26497 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-26220241322155.jpg "Immunoprecipitation: ATF4 Antibody (9E1) [NBP3-26497] - Immunoprecipitating ATF4 in Hela whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26497 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26497 (3ug) + Hela whole cell lysate (500ug). Lane 3: Hela whole cell lysate (20ug).")
![Immunohistochemistry: ATF4 Antibody (9E1) [NBP3-26497] - Image of ATF4 Antibody (9E1) diluted at 1:160 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-26220241510616.jpg)
![Western Blot: ATF4 Antibody (9E1) [NBP3-26497] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate. All lanes: ATF4 Antibody at 1.6ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 39 KDa Observed band size: 50 KDa](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-282202411472134.jpg)
![Immunocytochemistry/Immunofluorescence: ATF4 Antibody (9E1) [NBP3-26497] - Staining of HepG2 cells with ATF4 Antibody (9E1) at 1:53, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-28220241312211.jpg)
![Flow Cytometry: ATF4 Antibody (9E1) [NBP3-26497] - Overlay histogram showing Hela cells stained with ATF4 Antibody (9E1) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26497_rabbit-atf4-mab-9e1-282202414312786.jpg)
![Western Blot: eIF2A Antibody (8V8H7) [NBP3-16864] - Western blot analysis of extracts of various cell lines, using eIF2A Rabbit mAb (NBP3-16864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/eIF2A-Antibody-8V8H7-Western-Blot-NBP3-16864-img0004.jpg "Western Blot: eIF2A Antibody (8V8H7) [NBP3-16864] - Western blot analysis of extracts of various cell lines, using eIF2A Rabbit mAb (NBP3-16864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864] - Immunohistochemistry of paraffin-embedded mouse testis using eIF2A Rabbit mAb (NBP3-16864) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/eIF2A-Antibody-8V8H7-Immunohistochemistry-Paraffin-NBP3-16864-img0003.jpg)
![Western Blot: eIF2A Antibody (8V8H7) [NBP3-16864] - Western blot analysis of extracts of Rat lung, using eIF2A Rabbit mAb (NBP3-16864) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/eIF2A-Antibody-8V8H7-Western-Blot-NBP3-16864-img0005.jpg)
![Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864] - Immunohistochemistry of paraffin-embedded rat pancreas using eIF2A Rabbit mAb (NBP3-16864) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/eIF2A-Antibody-8V8H7-Immunohistochemistry-Paraffin-NBP3-16864-img0001.jpg)
![Immunohistochemistry-Paraffin: eIF2A Antibody (8V8H7) [NBP3-16864] -Analysis of paraffin-embedded Human tonsil tissue using eIF2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16864_rabbit-eif2a-mab-8v8h7-immunohistochemistry-paraffin-73202594938..png)