| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24389SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77109-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-17999SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-11775SS | |||
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using p14ARF antibody (NB200-111) with ECL detection method.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0007.jpg "Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using p14ARF antibody (NB200-111) with ECL detection method.")
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - Ponceau S staining of HeLa whole cell lysate using NB800-PC1.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0003.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - Analysis of STIM-1 antibody (NB110-60547) using HeLa whole cell lysate [NB800-PC1].](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0004.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of 30ug of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal Survivin antibody (NB 500-201) at 1ug/ml concentration. Detection was performed using ECL based method with 1 minute exposure time.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0005.jpg)
![Western Blot: HeLa Whole Cell Lysate [NB800-PC1] - WB analysis of HeLa whole cell lysate (NB800-PC1) using rabbit polyclonal NBS1 antibody (NB100-143) with detection employing ECL method.](http://images.novusbio.com/fullsize/HeLa-Whole-Cell-Lysate-Western-Blot-NB800-PC1-img0006.jpg)
![Western Blot: Ubiquitin Antibody (1B4-UB) [NB600-776] - GST-ubiquitin detected with Mouse anti-Ubiquitin.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-1B4-UB-Western-Blot-NB600-776-img0002.jpg "Western Blot: Ubiquitin Antibody (1B4-UB) [NB600-776] - GST-ubiquitin detected with Mouse anti-Ubiquitin.")
![Immunohistochemistry-Paraffin: Ubiquitin Antibody (1B4-UB) [NB600-776] - Staining of human tonsil.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-1B4-UB-Immunohistochemistry-Paraffin-NB600-776-img0003.jpg)
![Immunohistochemistry-Paraffin: Ubiquitin Antibody (1B4-UB) [NB600-776] - Human hypophysis.](http://images.novusbio.com/fullsize/Ubiquitin-Antibody-1B4-UB-Immunohistochemistry-Paraffin-NB600-776-img0001.jpg)
![Western Blot: ATG5 Antibody [NBP2-24389] - Western blot analysis of human APGL5 in T98G cell lysate in 1) absence, 2) presence of immunizing peptide using this antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/ATG5-Antibody-Western-Blot-NBP2-24389-img0001.jpg "Western Blot: ATG5 Antibody [NBP2-24389] - Western blot analysis of human APGL5 in T98G cell lysate in 1) absence, 2) presence of immunizing peptide using this antibody at 1 ug/mL.")
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP2-24389] - Immunohistochemistry analysis of human kidney using this antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP2-24389-img0002.jpg)
![Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Rat Testis Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202419592087.jpg "Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Rat Testis Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.")
![Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Mouse Testis.Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Nanog antibody at 2 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202420312999.jpg)
![Immunohistochemistry: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in Human Testis.Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-Nanog antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-271120241932686.jpg)
![Immunocytochemistry/ Immunofluorescence: Nanog Antibody - BSA Free [NBP1-77109] - Validation of Nanog in HeLa Cells.Immunofluorescent analysis of PFA-fixed HeLa cells labeling Nanog with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202419553033.jpg)
![Western Blot: Nanog Antibody - BSA Free [NBP1-77109] - Validation in Mouse and Rat Testis.Antibodies: Nanog, 2 u/mL, 1h incubation at RT in 8% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-271120241923639.gif)
![Western Blot: Nanog Antibody - BSA Free [NBP1-77109] - Validation in HeLa CellsLoading: 15 u of lysate. Antibodies: Nanog, 2 u/mL, 1 h incubation at RT in 8% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-77109_rabbit-polyclonal-nanog-antibody-2711202419463442.gif)
![Western Blot: BromoTag Antibody [NBP3-17999] - Testing of NBP3-17999 antibody on a BromoTag containing endogenous heterozygous HEK293 cell line, against a well-characterized commercial Brd2 antibody. BromoTag contains Brd4L387A mutation that was used as the original immunogen. Total protein ranged from 2.5-40 ug per well. A HEK293 WT cell line was used as a control. Ponceaus Stain was used as a loading control. NBP3-17999 is demonstrated to be a selective BromoTag antibody.](http://images.novusbio.com/fullsize/BromoTag-Antibody-Western-Blot-NBP3-17999-img0001.jpg "Western Blot: BromoTag Antibody [NBP3-17999] - Testing of NBP3-17999 antibody on a BromoTag containing endogenous heterozygous HEK293 cell line, against a well-characterized commercial Brd2 antibody. BromoTag contains Brd4L387A mutation that was used as the original immunogen. Total protein ranged from 2.5-40 ug per well. A HEK293 WT cell line was used as a control. Ponceaus Stain was used as a loading control. NBP3-17999 is demonstrated to be a selective BromoTag antibody.")
![Western Blot: ATG5 Antibody [NBP3-11775] - Total protein from A431, U2Os, NIH 3T3, N2A, L929, RAW, PC12, Human Colon, Rat Colon and Mouse Colon were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ATG5 (NB110-53818) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/ATG5-Antibody-Western-Blot-NBP3-11775-img0003.jpg "Western Blot: ATG5 Antibody [NBP3-11775] - Total protein from A431, U2Os, NIH 3T3, N2A, L929, RAW, PC12, Human Colon, Rat Colon and Mouse Colon were separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ATG5 (NB110-53818) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NBP3-11775] - PC12 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATG5 Antibody NBP3-11775 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11775-img0008.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP3-11775] - Analysis of a FFPE tissue section of mouse testis using 1:200 dilution of ATG5 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP3-11775-img0002.jpg)
![Flow Cytometry: ATG5 Antibody [NBP3-11775] - An intracellular stain was performed on Neuro2a cells with ATG5 Antibody NBP3-11775 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/ATG5-Antibody-Flow-Cytometry-NBP3-11775-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NBP3-11775] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATG5 Antibody NBP3-11775 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11775-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ATG5 Antibody [NBP3-11775] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-ATG5 Antibody NBP3-11775 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-11775-img0007.jpg)
![Immunohistochemistry-Paraffin: ATG5 Antibody [NBP3-11775] - Analysis of a FFPE tissue section of human testis using 1:200 dilution of ATG5 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/ATG5-Antibody-Immunohistochemistry-Paraffin-NBP3-11775-img0001.jpg)
![Flow Cytometry: ATG5 Antibody [NBP3-11775] - An intracellular stain was performed on THP-1 cells with ATG5 Antibody NBP3-11775 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/ATG5-Antibody-Flow-Cytometry-NBP3-11775-img0004.jpg)
![Western Blot: BRCA1 Antibody (8F7) [NBP1-41186] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/BRCA1-Antibody-8F7-Western-Blot-NBP1-41186-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: BRCA1 Antibody (8F7) [NBP1-41186] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: BRCA1 Antibody (8F7) [NBP1-41186] - Paraffin-embedded Breast ca, using BRCA1 antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/BRCA1-Antibody-8F7-Immunohistochemistry-NBP1-41186-img0002.jpg)
![Western Blot: BRCA1 Antibody (8F7) [NBP1-41186] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [8F7] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382 was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/antibody/nbp1-41186_mouse-monoclonal-brca1-antibody-8f7-western-blot-81202411325..jpg)
![Western Blot: Proteasome 19S 10B Antibody [NB100-1483] - Staining of HeLa (A), U251 (B), KNRK (C) with antibody at 1 ug/mL and NIH3T3 (D) cell lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-Western-Blot-NB100-1483-img0001.jpg "Western Blot: Proteasome 19S 10B Antibody [NB100-1483] - Staining of HeLa (A), U251 (B), KNRK (C) with antibody at 1 ug/mL and NIH3T3 (D) cell lysate with antibody at 0.3 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Flow Cytometry: Proteasome 19S 10B Antibody [NB100-1483] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-Flow-Cytometry-NB100-1483-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Proteasome 19S 10B Antibody [NB100-1483] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Proteasome-19S-10B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1483-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: Proteasome 19S 10B Antibody [NB100-1483] - RA190 causes accumulation of I kappa B alpha & NF-kappa B in the cytoplasm, co-localized with proteasomes. HepG2 cells were treated with DMSO, 2 μM RA190 for or 25 μM MG132 for 30 min. The cells were fixed, permeabilized & stained for I kappa B alpha a, proteasome b, NF-kappa B c & viewed by confocal fluorescence microscopy individually, or d merged, or e under phase contrast. a, b, c I kappa B alpha & NF-kappa B in RA190 & MG132-treated cells is significantly accumulated in cytoplasm, & apparently co-localized with the proteasome. The proportion of fluorescence in the cytoplasm versus the nucleus was quantified for f I kappa B alpha , or g NF-kappa B Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32375699), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1483_goat-polyclonal-proteasome-19s-10b-antibody-imgenex-img-3751-310202415171319.jpg)
![Western Blot: ATG5 Antibody (8L7X6) [NBP3-15854] - Western blot analysis of extracts of various cell lines, using ATG5 antibody (NBP3-15854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ATG5-Antibody-8L7X6-Western-Blot-NBP3-15854-img0003.jpg "Western Blot: ATG5 Antibody (8L7X6) [NBP3-15854] - Western blot analysis of extracts of various cell lines, using ATG5 antibody (NBP3-15854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Western Blot: ATG5 Antibody (8L7X6) [NBP3-15854] - Western blot analysis of extracts from normal (control) and ATG5 knockout (KO) 293T cells, using ATG5 antibody (NBP3-15854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ATG5-Antibody-8L7X6-Knockout-Validated-NBP3-15854-img0001.jpg)
![Immunoprecipitation: ATG5 Antibody (8L7X6) [NBP3-15854] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug ATG5 antibody (NBP3-15854). Western blot was performed from the immunoprecipitate using ATG5 (NBP3-15854) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/ATG5-Antibody-8L7X6-Immunoprecipitation-NBP3-15854-img0004.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-5220251726014.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Mouse colon tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517251919.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-5220251720294.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-5220251726015.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Rat colon tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517273524.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Human colon cancer tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517213515.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-522025171896.jpg)
![Immunocytochemistry/ Immunofluorescence: ATG5 Antibody (8L7X6) [ATG5] - Confocal imaging of paraffin-embedded Human colon tissue using [KO Validated] ATG5 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517284996.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded rat colon tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517152727.jpg)
![Immunohistochemistry: ATG5 Antibody (8L7X6) [ATG5] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using [KO Validated] ATG5 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15854_rabbit-atg5-mab-8l7x6-52202517172826.jpg)
![Western Blot: PSME4 Antibody [NBP2-22236] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22236 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/PSME4-Antibody-Western-Blot-NBP2-22236-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: PSME4 Antibody [NBP2-22236] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. NBP2-22236 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: PSME4 Antibody [NBP2-22236] - Human non-small cell lung cancer. Antibody: Affinity purified rabbit anti- PSME4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).](http://images.novusbio.com/fullsize/PSME4-Antibody-Immunohistochemistry-Paraffin-NBP2-22236-img0007.jpg)
![Immunoprecipitation: PSME4 Antibody [NBP2-22236] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. NBP2-22236 used for IP at 6 ug/mg lysate. PSME4 was also immunoprecipitated by rabbit anti-PSME4 antibody. For blotting immunoprecipitated PSME4, NBP2-22236 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/PSME4-Antibody-Immunoprecipitation-NBP2-22236-img0002.jpg)
![Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Knockout/KnockdownWild-type (WT) and PSME3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PSME3 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Western-Blot-NBP1-33498-img0018.jpg "<b>Genetic Strategies Validation. </b>Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Knockout/KnockdownWild-type (WT) and PSME3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PSME3 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Western-Blot-NBP1-33498-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33498-img0009.jpg)
![Immunohistochemistry-Paraffin: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Mouse cervix. PSME3 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Immunohistochemistry-Paraffin-NBP1-33498-img0016.jpg)
![Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Western-Blot-NBP1-33498-img0013.jpg)
![Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Sample (30 ug of whole cell lysate) A: PC-12 12% SDS PAGE, antibody diluted at 1:2000.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Western-Blot-NBP1-33498-img0014.jpg)
![Immunohistochemistry-Paraffin: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - CA922 xenograft, using PSME3 antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/PA28-Activator-gamma-Subunit-PSME3-Antibody-Immunohistochemistry-Paraffin-NBP1-33498-img0010.jpg)
![Western Blot: Caspase-8 Antibody [NBP2-15722] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Western-Blot-NBP2-15722-img0008.jpg "Western Blot: Caspase-8 Antibody [NBP2-15722] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Caspase-8 Antibody [NBP2-15722] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 8 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Caspase-8-Antibody-Western-Blot-NBP2-15722-img0012.jpg)
![Western Blot: Caspase-8 Antibody [NBP2-15722] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase-8 antibody (NBP2-15722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-91020242015729.jpg)
![Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP2-15722] - Caspase-8 antibody detects Caspase-8 protein at cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded human breast carcinoma.</br>Caspase-8 stained by Caspase-8 antibody (NBP2-15722) diluted at 1:1000.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-91020242001016.jpg)
![Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody [NBP2-15722] - Caspase-8 antibody detects Caspase-8 protein at cytoplasm by immunofluorescent analysis.<br>Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.<br>Green: Caspase-8 protein stained by Caspase-8 antibody (NBP2-15722) diluted at 1:100.<br>Blue: Hoechst 33342 staining.<br>Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-91020242063388.jpg)
![Immunocytochemistry/ Immunofluorescence: Caspase-8 Antibody [NBP2-15722] - Caspase-8 antibody detects Caspase-8 protein at cytoplasm by immunofluorescent analysis.</br>Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.</br>Green: Caspase-8 stained by Caspase-8 antibody (NBP2-15722) diluted at 1:100.</br>Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-91020242001090.jpg)
![Immunohistochemistry-Paraffin: Caspase-8 Antibody [NBP2-15722] - Caspase-8 antibody detects Caspase-8 protein at cytoplasm by immunohistochemical analysis.</br>Sample: Paraffin-embedded cat kidney.</br>Caspase-8 stained by Caspase-8 antibody (NBP2-15722) diluted at 1:500.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-91020242021975.jpg)
![Western Blot: Caspase-8 Antibody [NBP2-15722] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase-8 antibody (NBP2-15722) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15722_rabbit-polyclonal-caspase-8-antibody-910202420122084.jpg)
