| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38158-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-05379-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44977 | |||
| Azide and BSA Free | NBP2-34817-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34442-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48410 | |||
| Azide and BSA Free | NBP3-11546 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59628 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50040SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB5729-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54592-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26350-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26482-50ul | |||
![Immunohistochemistry: MAGEA1 Antibody [NBP3-38158] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using MAGEA1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38158_rabbit-magea1-pab-1012202411392946.jpg "Immunohistochemistry: MAGEA1 Antibody [NBP3-38158] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using MAGEA1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.")
![Immunohistochemistry: MAGEA1 Antibody [NBP3-38158] - Immunohistochemistry analysis of paraffin-embedded Human stomach cancer using MAGEA1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38158_rabbit-magea1-pab-1012202411392984.jpg)
![Immunohistochemistry: MAGEA1 Antibody [NBP3-38158] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using MAGEA1 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38158_rabbit-magea1-pab-1012202411381280.jpg)
![Immunocytochemistry/ Immunofluorescence: MAGEA1 Antibody [NBP3-38158] - Immunofluorescence analysis of HeLa cells using MAGEA1 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38158_rabbit-magea1-pab-1012202411343363.jpg)
![Western Blot: MAGEA1 Antibody [NBP3-38158] - Western blot analysis of lysates from 22Rv1 cells, using MAGEA1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38158_rabbit-magea1-pab-101220241142108.jpg)
![Western Blot: Chk1 [p Ser280] Antibody [NBP3-05379] - Western blot analysis of extracts of HT-29 cells, using Phospho-Chk1-Ser280 antibody (NBP3-05379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/Chk1-[p-Ser280]-Antibody-Western-Blot-NBP3-05379-img0005.jpg "Western Blot: Chk1 [p Ser280] Antibody [NBP3-05379] - Western blot analysis of extracts of HT-29 cells, using Phospho-Chk1-Ser280 antibody (NBP3-05379) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Immunohistochemistry-Paraffin: Chk1 [p Ser280] Antibody [NBP3-05379] - Immunohistochemistry of paraffin-embedded mouse brain using Chk1 antibody (NBP3-05379) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Chk1-[p-Ser280]-Antibody-Immunohistochemistry-Paraffin-NBP3-05379-img0003.jpg)
![Immunohistochemistry-Paraffin: Chk1 [p Ser280] Antibody [NBP3-05379] - Immunohistochemistry of paraffin-embedded rat heart using Chk1 antibody (NBP3-05379) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Chk1-[p-Ser280]-Antibody-Immunohistochemistry-Paraffin-NBP3-05379-img0001.jpg)
![Immunohistochemistry-Paraffin: Chk1 [p Ser280] Antibody [NBP3-05379] - Immunohistochemistry of paraffin-embedded human placenta using Chk1 antibody (NBP3-05379) at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Chk1-[p-Ser280]-Antibody-Immunohistochemistry-Paraffin-NBP3-05379-img0002.jpg)
![Western Blot: Chk1 [p Ser280] Antibody - Azide and BSA Free [Chk1] - Western blot analysis of lysates from 293T cells, using Chk1 Rabbit pAb at 1:600 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-05379_rabbit-polyclonal-chk1-p-ser280-antibody-52202516293974.jpg)
![Western Blot: p53 Antibody (SPM589) [NBP2-32891] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (SPM589)](http://images.novusbio.com/fullsize/p53-Antibody-SPM589-Western-Blot-NBP2-32891-img0004.jpg "Western Blot: p53 Antibody (SPM589) [NBP2-32891] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (SPM589)")
![Immunohistochemistry-Paraffin: p53 Antibody (SPM589) [NBP2-32891] - Formalin-fixed paraffin-embedded human bladder carcinoma stained with p53 monoclonal antibody (SPM589)](http://images.novusbio.com/fullsize/p53-Antibody-SPM589-Immunohistochemistry-Paraffin-NBP2-32891-img0003.jpg)
![Western Blot: Bcl-2 Antibody (SPM530) [NBP2-34389] - Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Antibody (SPM530).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-SPM530-Western-Blot-NBP2-34389-img0004.jpg "Western Blot: Bcl-2 Antibody (SPM530) [NBP2-34389] - Western Blot Analysis of human MCF-7 cell lysate using Bcl-2 Antibody (SPM530).")
![Immunohistochemistry-Paraffin: Bcl-2 Antibody (SPM530) [NBP2-34389] - Formalin-fixed, paraffin-embedded tonsil stained with Bcl-2 Mouse Monoclonal Antibody (SPM530).](http://images.novusbio.com/fullsize/Bcl-2-Antibody-SPM530-Immunohistochemistry-Paraffin-NBP2-34389-img0005.jpg)
![Immunohistochemistry-Paraffin: BIN1 Antibody (2F11) [NBP2-21675] - Mouse Anti-BIN1 antibody. Tissue: skeletal muscle from BIN-1 wild type and null mouse. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. BIN-1 antibody for 1hr at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN1 as precipitated brown signal.](http://images.novusbio.com/fullsize/BIN1-Antibody-2F11-Immunohistochemistry-Paraffin-NBP2-21675-img0001.jpg "Immunohistochemistry-Paraffin: BIN1 Antibody (2F11) [NBP2-21675] - Mouse Anti-BIN1 antibody. Tissue: skeletal muscle from BIN-1 wild type and null mouse. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. BIN-1 antibody for 1hr at RT. Secondary antibody: Peroxidase mouse secondary antibody at 1:10,000 for 45 min at RT. Localization: BIN1 is nuclear and cytoplasmic. Staining: BIN1 as precipitated brown signal.")
![Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Knockout-Validated-NBP2-44982-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-44982) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/817) [NBP2-44982] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (TRP/817).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Western-Blot-NBP2-44982-img0005.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/817) [NBP2-44982] - Human Colon Carcinoma stained with p53 Monoclonal Antibody (TRP/817)](http://images.novusbio.com/fullsize/p53-Antibody-TRP-817-Immunohistochemistry-Paraffin-NBP2-44982-img0001.jpg)
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Knockout-Validated-NBP2-59626-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and p53 knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-59626) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for p53 at approximately 53 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Protein Array: p53 Antibody (TRP/816) [NBP2-59626] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Protein-Array-NBP2-59626-img0001.jpg)
![Western Blot: p53 Antibody (TRP/816) [NBP2-59626] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Western-Blot-NBP2-59626-img0006.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (TRP/816) [NBP2-59626] - Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p53 Mouse Monoclonal Antibody (TRP/816).](http://images.novusbio.com/fullsize/p53-Antibody-TRP-816-Immunohistochemistry-Paraffin-NBP2-59626-img0002.jpg)
![Western Blot: Aurora A Antibody (1A11) [NBP2-50040] - Analysis of different cell lysates and recombinant protein solutions using mouse Aurora A mAb. Left: cells were treated with 100ng/mL of nocodazol (a microtubule depolymerizing agent which induces cells to halt at G2/M phase) for 6hrs: [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] Equine NBL6 cells, and [5] murine KR158 cells. The antibody apparently does not recognize canine or equine aurora A, but does bind human and murine. Right: equal amounts of purified human recombinant aurora A, B, C as indicated. The NBP2-50040 antibody reacts with the aurora A recombinant protein but not aurora B or C.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1A11-Western-Blot-NBP2-50040-img0005.jpg "Western Blot: Aurora A Antibody (1A11) [NBP2-50040] - Analysis of different cell lysates and recombinant protein solutions using mouse Aurora A mAb. Left: cells were treated with 100ng/mL of nocodazol (a microtubule depolymerizing agent which induces cells to halt at G2/M phase) for 6hrs: [1] protein standard, [2] HeLa, [3] canine A72 cells, [4] Equine NBL6 cells, and [5] murine KR158 cells. The antibody apparently does not recognize canine or equine aurora A, but does bind human and murine. Right: equal amounts of purified human recombinant aurora A, B, C as indicated. The NBP2-50040 antibody reacts with the aurora A recombinant protein but not aurora B or C.")
![Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1A11) [NBP2-50040] - HeLa cell cultures stained with NBP2-50040 (green). Aurora A localizes in spindle poles and mitotic spindles at late mitosis. Counterstained is chicken polyclonal antibody against Vimentin (NB300-223, red). Blue is a DNA stain.](http://images.novusbio.com/fullsize/Aurora-A-Antibody-1A11-Immunocytochemistry-Immunofluorescence-NBP2-50040-img0003.jpg)
![Immunocytochemistry: WT1 Antibody (960522) [MAB5729] - WT-1 was detected in immersion fixed K-562 cell line using MAB5729 at 8 ug/ml for 3 hours at room temperature. Cells were stained using NL007 (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplam & nuclei.](http://images.novusbio.com/fullsize/WT1-Antibody-960522-Immunocytochemistry-MAB5729-img0002.jpg "Immunocytochemistry: WT1 Antibody (960522) [MAB5729] - WT-1 was detected in immersion fixed K-562 cell line using MAB5729 at 8 ug/ml for 3 hours at room temperature. Cells were stained using NL007 (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplam & nuclei.")
![Flow Cytometry: WT1 Antibody (960522) [MAB5729] - An intracellular stain was performed on THP-1 cells with WT1 [960522] Antibody MAB5729AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/WT1-Antibody-960522-Flow-Cytometry-MAB5729-img0003.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (rWT1/857) [NBP2-53181] - Formalin-fixed, paraffin-embedded Rat Testis stained with WT1 Antibody (rWT1/857).](http://images.novusbio.com/fullsize/WT1-Antibody-rWT1-857-Immunohistochemistry-Paraffin-NBP2-53181-img0004.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (rWT1/857) [NBP2-53181] - Formalin-fixed, paraffin-embedded Rat Testis stained with WT1 Antibody (rWT1/857).")
![Immunohistochemistry-Paraffin: WT1 Antibody (rWT1/857) [NBP2-53181] - Formalin-fixed, paraffin-embedded human Testis stained with WT1 Antibody (rWT1/857).](http://images.novusbio.com/fullsize/WT1-Antibody-rWT1-857-Immunohistochemistry-Paraffin-NBP2-53181-img0002.jpg)
![Protein Array: WT1 Antibody (rWT1/857) [NBP2-53181] - Analysis of Protein Array containing more than 19,000 full-length human proteins using WT1 Antibody (rWT1/857).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/WT1-Antibody-rWT1-857-Protein-Array-NBP2-53181-img0005.jpg)
![Immunohistochemistry-Paraffin: WT1 Antibody (rWT1/857) [NBP2-53181] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (rWT1/857).](http://images.novusbio.com/fullsize/nbp2-53181_mouse-monoclonal-wt1-antibody-rwt1-857-1772024938132.jpg)
![Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-26220241422521.jpg "Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Image of RBBP4/RbAp48 Antibody (3E9) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-2722024850460.jpg)
![Western Blot: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Positive Western Blot detected in: Hela whole cell lysate, K562 whole cell lysate, HepG2 whole cell lysate, HEK293 whole cell lysate, L02 whole cell lysate, Jurkat whole cell lysate, SH-SY5Y whole cell lysate, Mouse Brain whole cell lysate, Rat Brain cell lysate. All lanes: RBBP4/RbAp48 Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 48, 48, 47, 44 kDa Observed band size: 53, 40 kDa](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-28220241135166.jpg)
![Immunocytochemistry/Immunofluorescence: RBBP4/RbAp48 Antibody (3E9) [NBP3-26350] - Staining of Hela Cells with RBBP4/RbAp48 Antibody (3E9) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26350_rabbit-rbbp4-rbap48-mab-3e9-282202412475121.jpg)
![Immunoprecipitation: BAK Antibody (8D1) [NBP3-26482] - Immunoprecipitating BAK in HEK293 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26482 in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26482 (3ug) + HEK293 whole cell lysate (500ug). Lane 3: HEK293 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/nbp3-26482_rabbit-bak-mab-8d1-262202413221514.jpg "Immunoprecipitation: BAK Antibody (8D1) [NBP3-26482] - Immunoprecipitating BAK in HEK293 whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26482 in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26482 (3ug) + HEK293 whole cell lysate (500ug). Lane 3: HEK293 whole cell lysate (20ug).")
![Immunohistochemistry: BAK Antibody (8D1) [NBP3-26482] - Image of BAK Antibody (8D1) diluted at 1:90 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26482_rabbit-bak-mab-8d1-2722024840450.jpg)
![Immunohistochemistry: BAK Antibody (8D1) [NBP3-26482] - Image of BAK Antibody (8D1) diluted at 1:90 and staining in paraffin-embedded human lymph node tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26482_rabbit-bak-mab-8d1-27220248573315.jpg)
![Western Blot: BAK Antibody (8D1) [NBP3-26482] - Positive Western Blot detected in: Hela whole cell lysate, MCF-7 whole cell lysate, THP-1 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, Colo320 whole cell lysate. All lanes: BAK Antibody at 0.9ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 24, 17 KDa Observed band size: 24 KDa](http://images.novusbio.com/fullsize/nbp3-26482_rabbit-bak-mab-8d1-28220241140912.jpg)
![Flow Cytometry: BAK Antibody (8D1) [NBP3-26482] - Overlay histogram showing Hela cells stained with BAK Antibody (8D1) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26482_rabbit-bak-mab-8d1-28220241418365.jpg)