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![Western Blot: Rad9 Antibody [NB100-57077] - Staining of A431cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Rad9-Antibody-Western-Blot-NB100-57077-img0001.jpg "Western Blot: Rad9 Antibody [NB100-57077] - Staining of A431cell lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: ING4 Antibody [NB100-1682] - Used at 1.0 ug/ml to detect ING4 (lane 2) present in a U2OS whole cell lysate over expressing the protein. A control lysate (lane 3) shows no background staining. Comparison to MW markers (lane 1) indicates detection of a single band at 29 kDa corresponding to ING4. A 4-20% TRIS-glycine gradient gel was used to separate the protein by SDS-PAGE under reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking using 5% non-fat dry milk in PBS, the membrane was probed with the primary antibody overnight at 4 C followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature.](http://images.novusbio.com/fullsize/ING4-Antibody-Western-Blot-NB100-1682-img0005.jpg "Western Blot: ING4 Antibody [NB100-1682] - Used at 1.0 ug/ml to detect ING4 (lane 2) present in a U2OS whole cell lysate over expressing the protein. A control lysate (lane 3) shows no background staining. Comparison to MW markers (lane 1) indicates detection of a single band at 29 kDa corresponding to ING4. A 4-20% TRIS-glycine gradient gel was used to separate the protein by SDS-PAGE under reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking using 5% non-fat dry milk in PBS, the membrane was probed with the primary antibody overnight at 4 C followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] for 45 min at room temperature.")
![Western Blot: p400 Antibody [NBP2-19663] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/p400-Antibody-Western-Blot-NBP2-19663-img0001.jpg "Western Blot: p400 Antibody [NBP2-19663] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: TRPM8 Antibody (SR1110) [NBP3-21832] - Western blot analysis using NBP3-21832 in A549 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21832_rabbit-trpm8-mab-sr1110-5720231526110.jpg "Western Blot: TRPM8 Antibody (SR1110) [NBP3-21832] - Western blot analysis using NBP3-21832 in A549 cell lysate.")
![Sandwich ELISA: SLC5A3 Antibody (3A6) [H00006526-M02] - Detection limit for recombinant GST tagged SLC5A3 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SLC5A3-Antibody-3A6-Sandwich-ELISA-H00006526-M02-img0001.jpg "Sandwich ELISA: SLC5A3 Antibody (3A6) [H00006526-M02] - Detection limit for recombinant GST tagged SLC5A3 is 0.3 ng/ml as a capture antibody.")
![ELISA: Human Granzyme A ELISA Kit (Colorimetric) [NBP3-11790] - Samples were spiked with high concentrations of Human Granzyme A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-11790_human-granzyme-a-elisa-kit-colorimetric-132202416232383.jpg "ELISA: Human Granzyme A ELISA Kit (Colorimetric) [NBP3-11790] - Samples were spiked with high concentrations of Human Granzyme A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Granzyme A ELISA Kit (Colorimetric) [NBP3-11790] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-11790_human-granzyme-a-elisa-kit-colorimetric-4320249163358.jpg)
![ELISA: Mouse Galectin-3 ELISA Kit (Colorimetric) [NBP2-67973] - Samples were spiked with high concentrations of Mouse Galectin-3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-67973_mouse-galectin-3-elisa-kit-colorimetric-132202416143089.jpg)
![ELISA: Mouse Galectin-3 ELISA Kit (Colorimetric) [NBP2-67973] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-67973_mouse-galectin-3-elisa-kit-colorimetric-432024912489.jpg)
![ELISA: Human c-jun ELISA Kit (Colorimetric) [NBP2-75279] - Samples were spiked with high concentrations of Human c-jun and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75279_human-c-jun-elisa-kit-colorimetric-132202416121342.jpg "ELISA: Human c-jun ELISA Kit (Colorimetric) [NBP2-75279] - Samples were spiked with high concentrations of Human c-jun and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human c-jun ELISA Kit (Colorimetric) [NBP2-75279] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75279_human-c-jun-elisa-kit-colorimetric-432024856721.jpg)
![ELISA: Rat Bcl-2 ELISA Kit (Colorimetric) [NBP2-69947] - Samples were spiked with high concentrations of Rat Bcl-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69947_rat-bcl-2-elisa-kit-colorimetric-13220241681467.jpg "ELISA: Rat Bcl-2 ELISA Kit (Colorimetric) [NBP2-69947] - Samples were spiked with high concentrations of Rat Bcl-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat Bcl-2 ELISA Kit (Colorimetric) [NBP2-69947] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69947_rat-bcl-2-elisa-kit-colorimetric-4320248582532.jpg)
![Western Blot: ING5 Antibody [NB100-1681] - Analysis is shown using Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in cell extracts. This western blot shows reactivity with purified recombinant human ING5 protein. Comparison to a molecular weight marker (not shown) indicates a single band of 36 kDa corresponding to the expected molecular weight for the recombinant protein. Approximately 10 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Incubation was overnight at 4 C followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.](http://images.novusbio.com/fullsize/ING5-Antibody-Western-Blot-NB100-1681-img0005.jpg "Western Blot: ING5 Antibody [NB100-1681] - Analysis is shown using Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in cell extracts. This western blot shows reactivity with purified recombinant human ING5 protein. Comparison to a molecular weight marker (not shown) indicates a single band of 36 kDa corresponding to the expected molecular weight for the recombinant protein. Approximately 10 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Incubation was overnight at 4 C followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.")
![Western Blot: ING5 Antibody [NB100-1681] - Analysis is shown using Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification is shown in lane 1. Comparison to a molecular weight marker (not shown) indicates a single band of 45.0 kDa corresponding to the expected molecular weight for the recombinant protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using chemiluminescence reagent with a 5-min exposure time.](http://images.novusbio.com/fullsize/ING5-Antibody-Western-Blot-NB100-1681-img0006.jpg)
![Western Blot: ErbB2/Her2 [p Tyr1139] Antibody [NBP3-12918] - Untreated (-) and treated (+) A431 whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1139) antibody (NBP3-12918) diluted at 1:10000.](http://images.novusbio.com/fullsize/ErbB2-Her2-[p-Tyr1139]-Antibody-Western-Blot-NBP3-12918-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: ErbB2/Her2 [p Tyr1139] Antibody [NBP3-12918] - Untreated (-) and treated (+) A431 whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2/Her2 (phospho Tyr1139) antibody (NBP3-12918) diluted at 1:10000.")
![<b>Biological Strategies Validation. </b>Western Blot: ErbB2/Her2 [p Tyr1139] Antibody [NBP3-12918] - ErbB2 (phospho Tyr1139) antibody detects ErbB2 (phospho Tyr1139) protein by western blot analysis. Un-treated (-) and treated (+, 200ng/ml Trichostatin A treatment for 15min) A431 whole cell extracts (5 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2 (phospho Tyr1139) antibody (NBP3-12918) at a dilution of 1:10000.](http://images.novusbio.com/fullsize/ErbB2-Her2-[p-Tyr1139]-Antibody-Western-Blot-NBP3-12918-img0001.jpg)