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![Western Blot: p400 Antibody [NBP2-19663] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/p400-Antibody-Western-Blot-NBP2-19663-img0001.jpg "Western Blot: p400 Antibody [NBP2-19663] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: TLK1 Antibody [NBP1-77982] - Shows detection of a band ~64 kDa corresponding to isoform 3 of human Tlk-1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 30 ug of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:250 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.](http://images.novusbio.com/fullsize/TLK1-Antibody-Western-Blot-NBP1-77982-img0002.jpg "Western Blot: TLK1 Antibody [NBP1-77982] - Shows detection of a band ~64 kDa corresponding to isoform 3 of human Tlk-1 (arrowhead). Specific reactivity with this band is blocked when the antibody is pre-incubated with the immunizing peptide (data not shown). Approximately 30 ug of a HeLa nuclear extract was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:250 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.")
![Western Blot: c-Rel Antibody [NBP1-52021] - Analysis of c-Rel in Human Spleen lysate (35ug protein in RIPA buffer) using this antibody at 0.5 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/c-Rel-Antibody-Western-Blot-NBP1-52021-img0002.jpg "Western Blot: c-Rel Antibody [NBP1-52021] - Analysis of c-Rel in Human Spleen lysate (35ug protein in RIPA buffer) using this antibody at 0.5 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Polycystin 2 Antibody [NBP1-51975] - (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Polycystin-2-Antibody-Western-Blot-NBP1-51975-img0001.jpg "Western Blot: Polycystin 2 Antibody [NBP1-51975] - (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Exonuclease 1 Antibody [NB100-93394] - (0.2ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Exonuclease-1-Antibody-Western-Blot-NB100-93394-img0002.jpg "Western Blot: Exonuclease 1 Antibody [NB100-93394] - (0.2ug/ml) staining of A549 cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: PHLPP2 Antibody [NB100-61668] - Staining of Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PHLPP2-Antibody-Western-Blot-NB100-61668-img0002.jpg "Western Blot: PHLPP2 Antibody [NB100-61668] - Staining of Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Rad9 [p Ser1260] Antibody [NBP1-77981] - Affinity purified Rad9 [p Ser1260] antibody used at a 1:200 dilution incubated overnight at 4C to detect Rad9 by Western blot. Lanes loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 by treatment with ATP & Rad53 kinase. Rad53 kinase autophosphorylates & appears cross reactive as it is detected on the blot as 90 & 110 kDa bands (asterisk). Detection occurred using a 1:5,000 IRDye(TM)800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan & process the image.](http://images.novusbio.com/fullsize/Rad9-[p-Ser1260]-Antibody-Western-Blot-NBP1-77981-img0003.jpg "Western Blot: Rad9 [p Ser1260] Antibody [NBP1-77981] - Affinity purified Rad9 [p Ser1260] antibody used at a 1:200 dilution incubated overnight at 4C to detect Rad9 by Western blot. Lanes loaded with 50 ng each of recombinant GST fusion protein containing S. cerevisiae Rad9 (aa 991-1309 ~60 kDa) on a 4-20% Criterion gel for SDS-PAGE as follows: Lane 1 - non-phosphorylated wild type yeast Rad9, Lane 2 - in vitro phosphorylated wild type yeast Rad9, Lane 3 - non-phosphorylated S1129A/S1260A double mutant Rad9, Lane 4 - in vitro phosphorylated S1129A/S1260A double mutant. Phosphorylation of Rad9 by treatment with ATP & Rad53 kinase. Rad53 kinase autophosphorylates & appears cross reactive as it is detected on the blot as 90 & 110 kDa bands (asterisk). Detection occurred using a 1:5,000 IRDye(TM)800 conjugated Donkey anti-Rabbit IgG for 1h at room temperature. LICOR's Odyssey(R) Infrared Imaging System was used to scan & process the image.")
![N/A: Rad9 [p Ser1260] Antibody [NBP1-77981]](http://images.novusbio.com/fullsize/Rad9-[p-Ser1260]-Antibody-N-A-NBP1-77981-img0002.jpg)
![Western Blot: BIN1 Antibody (12A) [NBP2-21689] - Mouse Anti-BIN-1 Antibody. Lane 1: LNCap. Lane 2: WM1341D. Lane 3: WM164. Lane 4: WM983A. Lane 5: WM793. Lane 6: WM239A. Lane 7: WM35. Load: 35 ug per lane. BIN-1 antibody at 1:400 for overnight at 4C. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blocking Buffer overnight at 4C.](http://images.novusbio.com/fullsize/BIN1-Antibody-12A-Western-Blot-NBP2-21689-img0001.jpg "Western Blot: BIN1 Antibody (12A) [NBP2-21689] - Mouse Anti-BIN-1 Antibody. Lane 1: LNCap. Lane 2: WM1341D. Lane 3: WM164. Lane 4: WM983A. Lane 5: WM793. Lane 6: WM239A. Lane 7: WM35. Load: 35 ug per lane. BIN-1 antibody at 1:400 for overnight at 4C. Secondary antibody: Dye800 mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blocking Buffer overnight at 4C.")
![Immunohistochemistry-Paraffin: WT1 Antibody (SPM361) [NBP2-44605] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (SPM361).](http://images.novusbio.com/fullsize/WT1-Antibody-SPM361-Immunohistochemistry-Paraffin-NBP2-44605-img0002.jpg "Immunohistochemistry-Paraffin: WT1 Antibody (SPM361) [NBP2-44605] - Formalin-fixed, paraffin-embedded human Mesothelioma stained with WT1 Antibody (SPM361).")
![Western Blot: BAK Antibody [NB100-893] - Staining of Human Adrenal Gland lysate (35 ug protein in RIPA buffer). BAK antibody at 2 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/BAK-Antibody-Western-Blot-NB100-893-img0001.jpg "Western Blot: BAK Antibody [NB100-893] - Staining of Human Adrenal Gland lysate (35 ug protein in RIPA buffer). BAK antibody at 2 ug/mL. Detected by chemiluminescence.")
![Western Blot: 53BP2 Antibody [NBP1-77907] - Detection of over-expressed ASPP2 in MCF-7 cells (Lane 2, arrowhead). Lane 1 is a nontransfected control. Lane 3 is MCF-7 cells over-expressing ASPP1. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. The identity of the lower MW band at approximately 40kDa is unknown.](http://images.novusbio.com/fullsize/53BP2-Antibody-Western-Blot-NBP1-77907-img0001.jpg "Western Blot: 53BP2 Antibody [NBP1-77907] - Detection of over-expressed ASPP2 in MCF-7 cells (Lane 2, arrowhead). Lane 1 is a nontransfected control. Lane 3 is MCF-7 cells over-expressing ASPP1. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. The identity of the lower MW band at approximately 40kDa is unknown.")
![Western Blot: ING1 Antibody [NB600-908] - Western blot analysis is shown using Affinity Purified ING1 antibody to detect over expressed Human ING1 present in cell nuclear extracts. This western blot shows reactivity with purified recombinant GST tagged human ING1b (lane 1, ~33kDa) and ING1c protein (lane 2, ~24kDa). No reactivity is seen against GST or other ING proteins (not shown). Reactivity of this polyclonal antibody is similar to the specificity of a positive control monoclonal antibody (not shown). The blot was incubated with a 1:1,000 dilution of the antibody at room temperature followed by detection using standard techniques.](http://images.novusbio.com/fullsize/ING1-Antibody-Western-Blot-NB600-908-img0006.jpg "Western Blot: ING1 Antibody [NB600-908] - Western blot analysis is shown using Affinity Purified ING1 antibody to detect over expressed Human ING1 present in cell nuclear extracts. This western blot shows reactivity with purified recombinant GST tagged human ING1b (lane 1, ~33kDa) and ING1c protein (lane 2, ~24kDa). No reactivity is seen against GST or other ING proteins (not shown). Reactivity of this polyclonal antibody is similar to the specificity of a positive control monoclonal antibody (not shown). The blot was incubated with a 1:1,000 dilution of the antibody at room temperature followed by detection using standard techniques.")
![N/A: ING1 Antibody [NB600-908]](http://images.novusbio.com/fullsize/ING1-Antibody-N-A-NB600-908-img0005.jpg)