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![Western Blot:[NBP3-29063]-Detection of human and mouse NPM1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NPM1 antibody used for WB at 0. 04 µg/ml and 0. 1 µg/ml and used for IP at 10 µg/mg lysate. NPM1 was successfully immunoprecipitated by rabbit anti-NPM1 antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds and 3 seconds.](http://images.novusbio.com/fullsize/nbp3-29063_rabbit-npm1-pab-23120251204028.jpg "Western Blot:[NBP3-29063]-Detection of human and mouse NPM1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa , HEK293T , and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-NPM1 antibody used for WB at 0. 04 µg/ml and 0. 1 µg/ml and used for IP at 10 µg/mg lysate. NPM1 was successfully immunoprecipitated by rabbit anti-NPM1 antibodies and , which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds and 3 seconds.")
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP63/11) [NBP3-07431] - Formalin-fixed, paraffin-embedded Mouse Prostate stained with p63/TP73L Mouse Monoclonal Antibody (TP63/11).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-TP63-11-Immunohistochemistry-Paraffin-NBP3-07431-img0002.jpg "Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP63/11) [NBP3-07431] - Formalin-fixed, paraffin-embedded Mouse Prostate stained with p63/TP73L Mouse Monoclonal Antibody (TP63/11).")
![Immunohistochemistry-Paraffin: p63/TP73L Antibody (TP63/11) [NBP3-07431] - Formalin-fixed, paraffin-embedded human Prostate Cancer stained with p63/TP73L Mouse Monoclonal Antibody (TP63/11).](http://images.novusbio.com/fullsize/p63-TP73L-Antibody-TP63-11-Immunohistochemistry-Paraffin-NBP3-07431-img0001.jpg)
![SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric) [NBP3-24485] - Standard Reference Curve](http://images.novusbio.com/fullsize/nbp3-24485_sod1-cu-zn-sod-activity-assay-kit-colorimetric-2611202315565818.png "SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric) [NBP3-24485] - Standard Reference Curve")
![SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric) [NBP3-24485] - Assay Example](http://images.novusbio.com/fullsize/nbp3-24485_sod1-cu-zn-sod-activity-assay-kit-colorimetric-812024113115.png)
![SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric) [NBP3-24485] - Assay Example](http://images.novusbio.com/fullsize/nbp3-24485_sod1-cu-zn-sod-activity-assay-kit-colorimetric-8120241104524.png)
![Western Blot: EGFR Antibody (HL1022) - L858R Mutant [NBP3-13683] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [HL1022] (NBP3-13683) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13683_rabbit-egfr-mab-hl1022-l858r-mutant-910202420115098.jpg "Western Blot: EGFR Antibody (HL1022) - L858R Mutant [NBP3-13683] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [HL1022] (NBP3-13683) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: EGFR Antibody (HL1022) - L858R Mutant [NBP3-13683] - Various whole cell extracts (30 | 15 | 15 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with EGFR antibody [HL1022] (NBP3-13683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13683_rabbit-egfr-mab-hl1022-l858r-mutant-91020242063323.jpg)
![ELISA: Human ATM ELISA Kit (Colorimetric) [NBP2-69891] - Samples were spiked with high concentrations of Human ATM and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-69891_human-atm-elisa-kit-colorimetric-132202416133650.jpg "ELISA: Human ATM ELISA Kit (Colorimetric) [NBP2-69891] - Samples were spiked with high concentrations of Human ATM and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human ATM ELISA Kit (Colorimetric) [NBP2-69891] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-69891_human-atm-elisa-kit-colorimetric-432024903543.jpg)
![Western Blot: p53 [p Ser6] Antibody [NBP3-13310] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser6) antibody (NBP3-13310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p53-[p-Ser6]-Antibody-Western-Blot-NBP3-13310-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 [p Ser6] Antibody [NBP3-13310] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser6) antibody (NBP3-13310) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: p53 [p Ser6] Antibody [NBP3-13310] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with p53 (phospho Ser6) antibody (NBP3-13310) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/p53-[p-Ser6]-Antibody-Western-Blot-NBP3-13310-img0001.jpg)
![Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Blooms Syndrome Protein Blm antibody [HL2771] (NBP3-48729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48729_rabbit-blooms-syndrome-protein-blm-mab-hl2771-western-blot-1411202518392011.jpg "<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Blooms Syndrome Protein Blm antibody [HL2771] (NBP3-48729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Blooms Syndrome Protein Blm antibody [HL2771] (NBP3-48729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48729_rabbit-blooms-syndrome-protein-blm-mab-hl2771-western-blot-14112025184051.jpg)
![Immunoprecipitation:[NBP3-30522]-Detection of human BLM by western blot of immunoprecipitates. Samples: Whole cell lysate from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLM antibody used for IP at 6 µg per reaction. BLM was also immunoprecipitated by a previous lot of this antibody and a second antibody against a different epitope of BLM. For blotting immunoprecipitated BLM, was used at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/nbp3-30522_rabbit-blooms-syndrome-protein-blm-pab-2312025129511.jpg "Immunoprecipitation:[NBP3-30522]-Detection of human BLM by western blot of immunoprecipitates. Samples: Whole cell lysate from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BLM antibody used for IP at 6 µg per reaction. BLM was also immunoprecipitated by a previous lot of this antibody and a second antibody against a different epitope of BLM. For blotting immunoprecipitated BLM, was used at 0. 04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric) [NBP3-24484] - Standard Reference Curve](http://images.novusbio.com/fullsize/nbp3-24484_sod1-cu-zn-sod-activity-assay-kit-colorimetric-2611202315565832.png "SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric) [NBP3-24484] - Standard Reference Curve")
![SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric) [NBP3-24484] - Assay Example](http://images.novusbio.com/fullsize/nbp3-24484_sod1-cu-zn-sod-activity-assay-kit-colorimetric-81202410594347.png)
![SOD1/Cu-Zn SOD Activity Assay Kit (Colorimetric) [NBP3-24484] - Assay Example](http://images.novusbio.com/fullsize/nbp3-24484_sod1-cu-zn-sod-activity-assay-kit-colorimetric-8120241104538.png)
![Immunohistochemistry-Paraffin: RB1 Antibody (rRB1/8060) [NBP3-23828] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with RB1 Antibody (rRB1/8060). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/nbp3-23828_mouse-rb1-mab-rrb1-8060-2412025863671.jpg "Immunohistochemistry-Paraffin: RB1 Antibody (rRB1/8060) [NBP3-23828] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with RB1 Antibody (rRB1/8060). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.")
![Western Blot: ERK2 Knockout A549 Cell Lysate [NBP3-18632] - Lane 1: Opal Prestained MW Marker Lane 2: A549 WCL Parental. Lane 3: A549 MAPK1 KO Clone 4. Lane 4: A549 MAPK1 KO Clone 10. Lane 5: A549 MAPK1 KO Clone 15. Lane 6: HeLa WCL Parental Load: 10ug lysate/lane. Primary Antibody [Blot A] Anti-MAPK1(ERK2) ~44kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:30,000 for 1hr at RT. Block: BlockOut Buffer for 1hr at RT. No detection of expected band at ~44kDa is observed in MAPK1 (ERK2) knockout A549 when compared with unmodified A549 cell lysates by Western blot.](http://images.novusbio.com/fullsize/ERK2-Knockout-A549-Cell-Lysate-Western-Blot-NBP3-18632-img0001.jpg "Western Blot: ERK2 Knockout A549 Cell Lysate [NBP3-18632] - Lane 1: Opal Prestained MW Marker Lane 2: A549 WCL Parental. Lane 3: A549 MAPK1 KO Clone 4. Lane 4: A549 MAPK1 KO Clone 10. Lane 5: A549 MAPK1 KO Clone 15. Lane 6: HeLa WCL Parental Load: 10ug lysate/lane. Primary Antibody [Blot A] Anti-MAPK1(ERK2) ~44kDa; [Blot B] stripped and re-probed with Anti-Tubulin ~50kDa; at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:30,000 for 1hr at RT. Block: BlockOut Buffer for 1hr at RT. No detection of expected band at ~44kDa is observed in MAPK1 (ERK2) knockout A549 when compared with unmodified A549 cell lysates by Western blot.")