| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75978SS | |||
| BSA Free | NBP2-75978 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61764-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77462SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72569 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89201-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84826-25ul | |||
![Western Blot: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Western-Blot-NBP2-71615-img0002.jpg "Western Blot: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IGF2BP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IGF2BP2.")
![Immunocytochemistry/Immunofluorescence: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY IGF2BP2.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71615-img0005.jpg)
![Immunohistochemistry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of paraffin-embedded Human Kidney tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71615-img0009.jpg)
![Flow Cytometry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-IGF2BP2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71615-img0004.jpg)
![Western Blot: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Analysis of extracts (35ug) from 9 different cell lines by using anti-IGF2BP2 monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Western-Blot-NBP2-71615-img0001.jpg)
![Immunohistochemistry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71615-img0006.jpg)
![Immunohistochemistry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71615-img0007.jpg)
![Immunohistochemistry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71615-img0008.jpg)
![Immunohistochemistry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Staining of paraffin-embedded Human liver tissue using anti-IGF2BP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71615-img0010.jpg)
![Flow Cytometry: IMP2/IGF2BP2 Antibody (OTI3F9) - Azide and BSA Free [NBP2-71615] - Analysis of Jurkat cells, using anti-IGF2BP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-OTI3F9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-71615-img0003.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of RUNX2/CBFA1 on different lysates with Rabbit anti-RUNX2/CBFA1 antibody at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Western-Blot-NBP2-67777-img0005.jpg "Western Blot: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of RUNX2/CBFA1 on different lysates with Rabbit anti-RUNX2/CBFA1 antibody at 1/500 dilution. Lane 1: HL-60 cell lysate Lane 2: Jurkat cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 57 kDa Observed band size: 57 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Staining RUNX2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunocytochemistry-Immunofluorescence-NBP2-67777-img0002.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded mouse bone tissue with Rabbit anti-RUNX2/CBFA1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0009.jpg)
![Western Blot: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of RUNX2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse spleen tissue lysateLane 2: U937 cell lysate Lane 3: HL-60 cell lysate](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Western-Blot-NBP2-67777-img0003.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human tonsil tissue using anti-RUNX2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0001.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RUNX2/CBFA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0004.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human tonsil tissue using anti-RUNX2/CBFA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0006.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-RUNX2/CBFA1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0007.jpg)
![Immunohistochemistry-Paraffin: RUNX2/CBFA1 Antibody (SD208-0) [NBP2-67777] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-RUNX2/CBFA1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-SD208-0-Immunohistochemistry-Paraffin-NBP2-67777-img0008.jpg)
![Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Western-Blot-NBP2-80765-img0001.jpg "Western Blot: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80765-img0002.jpg)
![Immunohistochemistry: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - HIF-1 alpha (clone 2443C) was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) at 1.0 ug/mL for 1 hour at room temperature followed by incubation with the](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80765-img0003.jpg)
![Simple Western: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20 ug/mL. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Simple-Western-NBP2-80765-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: HIF-1 alpha Antibody (2443C) - Azide and BSA Free [NBP2-80765] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (Catalog # NBP2-75978) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443C-Azide-and-BSA-Free-Knockout-Validated-NBP2-80765-img0004.jpg)
![Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against human Bmi1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Western-Blot-NBP2-22204-img0007.jpg "Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against human Bmi1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of Hela cells using Bmi1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunocytochemistry-Immunofluorescence-NBP2-22204-img0002.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded rectum cancer tissues using Bmi1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunohistochemistry-Paraffin-NBP2-22204-img0004.jpg)
![Flow Cytometry: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of NIH/3T3 cells using Bmi1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Flow-Cytometry-NBP2-22204-img0001.jpg)
![Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Western-Blot-NBP2-22204-img0006.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded cervical cancer tissues using Bmi1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-Immunohistochemistry-Paraffin-NBP2-22204-img0003.jpg)
![ELISA: BMI-1 Antibody (3E3) [NBP2-22204] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/BMI-1-Antibody-3E3-ELISA-NBP2-22204-img0005.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0008.jpg "Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against HEK293 (1) and ESR1 (AA: 2-185)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunocytochemistry-Immunofluorescence-NBP2-61764-img0004.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of esophageal cancer tissues using ESR1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunohistochemistry-NBP2-61764-img0006.jpg)
![Flow Cytometry: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-ELISA-NBP2-61764-img0002.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mAb against human ESR1 (AA: 2-185) recombinant protein. (Expected MW is 45.8 kDa)](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0007.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis using ESR1 mouse mAb against MOLT4 (1), Raji (2), MCF-7 (3), T47D (4), SK-Br-3 (5), Hela (6), and C6 (7) cell lysate.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Western-Blot-NBP2-61764-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of Hela cells using ESR1 mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunocytochemistry-Immunofluorescence-NBP2-61764-img0003.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Analysis of breast cancer tissues using ESR1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-Immunohistochemistry-NBP2-61764-img0005.jpg)
![ELISA: ER alpha/NR3A1 Antibody (6A6E8) [NBP2-61764] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-6A6E8-ELISA-NBP2-61764-img0001.jpg)
![Immunohistochemistry: RUNX2/CBFA1 Antibody [NBP1-77462] - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunohistochemistry-NBP1-77462-img0005.jpg "Immunohistochemistry: RUNX2/CBFA1 Antibody [NBP1-77462] - Staining of RUNX2 in mouse prostate using DAB with hematoxylin counterstain.")
![Immunocytochemistry/Immunofluorescence: RUNX2/CBFA1 Antibody [NBP1-77462] - RUNX2 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77462-img0006.jpg)
![Flow Cytometry: RUNX2/CBFA1 Antibody [NBP1-77462] - An intracellular stain was performed on HeLa cells with RUNX2/CBFA1 Antibody NBP1-77462AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/RUNX2-CBFA1-Antibody-Flow-Cytometry-NBP1-77462-img0007.jpg)
![Western Blot: MafA Antibody [NBP2-24636] - Analysis of MafA in human liver lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver lysate and 4) rat liver lysate using this antibody. 25 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/MafA-Antibody-Western-Blot-NBP2-24636-img0001.jpg "Western Blot: MafA Antibody [NBP2-24636] - Analysis of MafA in human liver lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver lysate and 4) rat liver lysate using this antibody. 25 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - Human breast cancer, using mtTFA antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Immunohistochemistry-Paraffin-NBP2-19438-img0009.jpg "Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - Human breast cancer, using mtTFA antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/mtTFA-Antibody-Western-Blot-NBP2-19438-img0010.jpg)
![Western Blot: mtTFA Antibody [NBP2-19438] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody (NBP2-19438) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19438_rabbit-polyclonal-mttfa-antibody-9102024205611.jpg)
![Immunohistochemistry-Paraffin: mtTFA Antibody [NBP2-19438] - mtTFA antibody detects mtTFA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human esophageal carcinoma.mtTFA stained by mtTFA antibody (NBP2-19438) diluted at 1:165.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-19438_rabbit-polyclonal-mttfa-antibody-91020242014635.jpg)
![Western Blot: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Western-Blot-NBP2-45718-img0017.jpg "Western Blot: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAFB.")
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human lung tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0016.jpg)
![Flow Cytometry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by MAFB antibody.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Flow-Cytometry-NBP2-45718-img0001.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human breast tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0002.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human pancreas tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0003.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0004.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human Ovary tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0005.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0006.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0007.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human bladder tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0008.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0009.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0010.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human breast tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0011.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0012.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0013.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0014.jpg)
![Immunohistochemistry: MafB Antibody (OTI1E9) [NBP2-45718] - Analysis of Adenocarcinoma of Human colon tissue.](http://images.novusbio.com/fullsize/MafB-Antibody-OTI1E9-Immunohistochemistry-NBP2-45718-img0015.jpg)
![Western Blot: Olig2 Antibody (064) [NBP2-89201] - A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:37 kDa](http://images.novusbio.com/fullsize/Olig2-Antibody-064-Western-Blot-NBP2-89201-img0003.jpg "Western Blot: Olig2 Antibody (064) [NBP2-89201] - A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:37 kDa")
![Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201] - Staining of human olig-2 in human brain with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Olig2-Antibody-064-Immunohistochemistry-Paraffin-NBP2-89201-img0002.jpg)
![Immunohistochemistry-Paraffin: Olig2 Antibody (064) [NBP2-89201] - Staining of human olig-2 in human glioma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/Olig2-Antibody-064-Immunohistochemistry-Paraffin-NBP2-89201-img0001.jpg)
![Flow Cytometry: Rabbit Monoclonal Olig2 Antibody (064) [NBP2-89201] - Mouse brain cells fixed and stained for Olig2 protein: 1:100. AF405 conjugated version of antibody used (Catalog # NBP2-89201AF405). Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-89201_rabbit-monoclonal-olig2-antibody-064-flow-cytometry-1052024144135..png)
![Flow Cytometry: Rabbit Monoclonal Olig2 Antibody (064) [NBP2-89201] - Rat brain cells fixed and stained with Olig2. AF405 conjugated version of antibody used (Catalog # NBP2-89201AF405). Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-89201_rabbit-monoclonal-olig2-antibody-064-flow-cytometry-1052024144528..png)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human cerebral cortex, cervix, uterine, endometrium and gastrointestinal using Anti-ER alpha/NR3A1 antibody NBP1-84826 (A) shows similar protein distribution across tissues to independent antibody NBP1-84827 (B).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0016.jpg)
![Western Blot: ER alpha/NR3A1 Antibody [NBP1-84826] - Analysis in control (vector only transfected HEK293T lysate) and ESR1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Western-Blot-NBP1-84826-img0007.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0019.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0014.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0017.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human small intestine shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/ER-alpha-NR3A1-Antibody-Immunohistochemistry-Paraffin-NBP1-84826-img0018.jpg)
![SDS-Page: Recombinant Human HIF-1 alpha Protein [H00003091-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-HIF-1-alpha-Protein-SDS-Page-H00003091-P01-img0002.jpg "SDS-Page: Recombinant Human HIF-1 alpha Protein [H00003091-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")