| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-153SS | |||
| Azide and BSA Free | NBP2-80870 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33549-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37822-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74378 | |||
![Western Blot: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in skeletal muscle lysate. Lane 1 shows pre-immune sera. Lanes 2, 3 and 4 show this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Western-Blot-NBP2-24693-img0006.jpg "Western Blot: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in skeletal muscle lysate. Lane 1 shows pre-immune sera. Lanes 2, 3 and 4 show this antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunocytochemistry/Immunofluorescence: BMI-1 Antibody [NBP2-24693] - Immunofluorescence of BMI1 in human oral squamous cell carcinoma (UPCI:SCC029B) cells. These cells are part of a large collection of HNSCC (head and neck SCC) lines established by the Gollin lab (White et al., 2007). The BMI1 antibody was used at 5 ug/ml. Staining is primarily nuclear, some cytoplasmic staining is also observed. Data courtesy of Hatem Kaseb, MD and Susanne M. Gollin, PhD.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-24693-img0004.jpg)
![Immunohistochemistry-Paraffin: BMI-1 Antibody [NBP2-24693] - Analysis of BMI1 in formalin-fixed, paraffin-embedded human cervix tissue using an isotype control (top left) and this antibody (bottom left, right) at 5 ug/ml.](http://images.novusbio.com/fullsize/BMI-1-Antibody-Immunohistochemistry-Paraffin-NBP2-24693-img0005.jpg)
![Western Blot: E6AP/UBE3A Antibody [NB500-240] - UBE3A Antibody [NB500-240] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-E6AP antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E6AP was also immunoprecipitated by rabbit anti-E6AP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).](http://images.novusbio.com/fullsize/E6AP-UBE3A-Antibody-Western-Blot-NB500-240-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: E6AP/UBE3A Antibody [NB500-240] - UBE3A Antibody [NB500-240] - Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-E6AP antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. E6AP was also immunoprecipitated by rabbit anti-E6AP antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).")
![Immunocytochemistry/Immunofluorescence: NFYA Antibody [NBP2-19533] - NFYA antibody detects NFYA protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NFYA stained by NFYA antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.](http://images.novusbio.com/fullsize/NFYA-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19533-img0006.jpg)
![Immunohistochemistry-Paraffin: NFYA Antibody [NBP2-19533] - Immunohistochemical analysis of paraffin-embedded D54 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/NFYA-Antibody-Immunohistochemistry-Paraffin-NBP2-19533-img0003.jpg)
![Western Blot: NFYA Antibody [NBP2-19533] - Sample (30 ug of whole cell lysate) A: A431 B: HeLa C: HepG2 10% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/NFYA-Antibody-Western-Blot-NBP2-19533-img0001.jpg)
![Western Blot: NFYA Antibody [NBP2-19533] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:3000.](http://images.novusbio.com/fullsize/NFYA-Antibody-Western-Blot-NBP2-19533-img0002.jpg)
![Western Blot: NFYA Antibody [NBP2-19533] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NFYA antibody diluted at 1:3000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/NFYA-Antibody-Western-Blot-NBP2-19533-img0007.jpg)
![Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Western-Blot-NB300-153-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - Specificity of Neurofibromin, using NB 300-153, in Schwann cells from embryonic knockout mice, adult human nerves and neonatal rat nerves.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Neurofibromin 1 Antibody (McNFn27a) [NB300-153] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human brain using Neurofibromin 1 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear staining of Neurofibromin 1was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-McNFn27a-Immunohistochemistry-Paraffin-NB300-153-img0005.jpg)
![Western Blot: SP1 Antibody (1A5) [H00006667-M02] - SP1 monoclonal antibody (M02), clone 1A5 Analysis of SP1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/SP1-Antibody-1A5-Western-Blot-H00006667-M02-img0014.jpg "Western Blot: SP1 Antibody (1A5) [H00006667-M02] - SP1 monoclonal antibody (M02), clone 1A5 Analysis of SP1 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: SP1 Antibody (1A5) [H00006667-M02] - Analysis of monoclonal antibody to SP1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SP1-Antibody-1A5-Immunocytochemistry-Immunofluorescence-H00006667-M02-img0008.jpg)
![Immunohistochemistry-Paraffin: SP1 Antibody (1A5) [H00006667-M02] - Analysis of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human colon. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/SP1-Antibody-1A5-Immunohistochemistry-Paraffin-H00006667-M02-img0012.jpg)
![Western Blot: SP1 Antibody (1A5) [H00006667-M02] - SP1 monoclonal antibody (M02), clone 1A5. Analysis of SP1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/SP1-Antibody-1A5-Western-Blot-H00006667-M02-img0015.jpg)
![Immunohistochemistry-Paraffin: SP1 Antibody (1A5) [H00006667-M02] - Analysis of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human endometrium. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/SP1-Antibody-1A5-Immunohistochemistry-Paraffin-H00006667-M02-img0010.jpg)
![ELISA: SP1 Antibody (1A5) [H00006667-M02] - Detection limit for recombinant GST tagged SP1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SP1-Antibody-1A5-ELISA-H00006667-M02-img0016.jpg)
![Immunohistochemistry-Paraffin: BCL11B Antibody [NBP2-33549] - Analysis in human skin and pancreas tissues. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/BCL11B-Antibody-Immunohistochemistry-Paraffin-NBP2-33549-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: BCL11B Antibody [NBP2-33549] - Analysis in human skin and pancreas tissues. Corresponding RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: BCL11B Antibody [NBP2-33549] - Staining of human cell line MCF7 shows localization to nucleus & nucleoli fibrillar center. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/BCL11B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33549-img0010.jpg)
![Immunohistochemistry-Paraffin: BCL11B Antibody [NBP2-33549] - Staining of human cerebral cortex shows weak to moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/BCL11B-Antibody-Immunohistochemistry-Paraffin-NBP2-33549-img0023.jpg)
![Immunohistochemistry: BCL11B Antibody [NBP2-33549] - Staining of mouse basal forebrain shows nuclear positivity in neurons of caudate putamen.](http://images.novusbio.com/fullsize/BCL11B-Antibody-Immunohistochemistry-Paraffin-NBP2-33549-img0011.jpg)
![Immunohistochemistry: BCL11B Antibody [NBP2-33549] - Staining of mouse nucleus accumbens shows nuclear positivity in neurons.](http://images.novusbio.com/fullsize/BCL11B-Antibody-Immunohistochemistry-Paraffin-NBP2-33549-img0013.jpg)
![Immunohistochemistry: BCL11B Antibody [NBP2-33549] - Staining of mouse parietal association cortex shows nuclear immunoreactivity in neurons.](http://images.novusbio.com/fullsize/BCL11B-Antibody-Immunohistochemistry-Paraffin-NBP2-33549-img0014.jpg)
![Immunohistochemistry-Paraffin: BCL11B Antibody [NBP2-33549] - Staining of human lymph node shows strong nuclear positivity in subsets of non-germinal center cells.](http://images.novusbio.com/fullsize/BCL11B-Antibody-Immunohistochemistry-Paraffin-NBP2-33549-img0018.jpg)
![Immunohistochemistry: BCL11B Antibody [NBP2-33549] - Staining of mouse brain shows moderate to strong positivity in more than 75 % of neurons in the olfactory bulb.](http://images.novusbio.com/fullsize/BCL11B-Antibody-Immunohistochemistry-NBP2-33549-img0020.jpg)
![Immunohistochemistry-Paraffin: BCL11B Antibody [NBP2-33549] - Staining of human pancreas shows no nuclear positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/BCL11B-Antibody-Immunohistochemistry-Paraffin-NBP2-33549-img0021.jpg)
![Immunohistochemistry-Paraffin: BCL11B Antibody [NBP2-33549] - Staining of human skin shows moderate to strong nuclear positivity in epidermal cells.](http://images.novusbio.com/fullsize/BCL11B-Antibody-Immunohistochemistry-Paraffin-NBP2-33549-img0022.jpg)
![Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249413344.jpg "Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249371524.jpg)
![Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249393512.jpg)
![Immunocytochemistry/Immunofluorescence: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249374953.jpg)
![Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - Human tissue extract (30 ug) was separatedby 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249374946.jpg)
![Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-212024939154.jpg)
![Immunohistochemistry-Paraffin: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - KAP1 antibody [HL1539] detects KAP1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. KAP1 stained by KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-212024940860.jpg)
![Western Blot: KAP1 Antibody (HL1539) - Azide and BSA Free [NBP3-25540] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with KAP1 antibody [HL1539] (NBP3-25540) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25540_rabbit-kap1-mab-hl1539-azide-and-bsa-free-2120249383124.jpg)
![Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Western-Blot-NBP3-13304-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: c-jun [p Ser73] Antibody [NBP3-13304] - c-jun (phospho Ser73) antibody detects c-jun (phospho Ser73) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. c-jun (phospho Ser73) stained by c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Immunohistochemistry-Paraffin-NBP3-13304-img0007.jpg)
![Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Western-Blot-NBP3-13304-img0002.jpg)
![Western Blot: c-jun [p Ser73] Antibody [NBP3-13304] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Western-Blot-NBP3-13304-img0003.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser73] Antibody [NBP3-13304] - c-jun (phospho Ser73) antibody detects c-jun (phospho Ser73) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. c-jun (phospho Ser73) stained by c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Immunohistochemistry-Paraffin-NBP3-13304-img0005.jpg)
![Immunohistochemistry-Paraffin: c-jun [p Ser73] Antibody [NBP3-13304] - c-jun (phospho Ser73) antibody detects c-jun (phospho Ser73) protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. c-jun (phospho Ser73) stained by c-jun (phospho Ser73) antibody (NBP3-13304) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/c-jun-[p-Ser73]-Antibody-Immunohistochemistry-Paraffin-NBP3-13304-img0006.jpg)
![Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249374982.jpg "Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249383186.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog tissue. Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249355536.jpg)
![Western Blot: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249403876.jpg)
![Immunocytochemistry/Immunofluorescence: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cytoplasm and nucleus and mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-212024937157.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded cat brain. Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-2120249415929.jpg)
![Immunohistochemistry-Paraffin: Thymidylate Synthase Antibody (HL1236) - Azide and BSA Free [NBP3-25721] - Thymidylate synthase antibody [HL1236] detects Thymidylate synthase protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded dog brain. Thymidylate synthase stained by Thymidylate synthase antibody [HL1236] (NBP3-25721) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25721_rabbit-thymidylate-synthase-mab-hl1236-azide-bsa-free-212024941337.jpg)
![Western Blot: CREB Antibody [NBP2-22516] - Analysis of GH4 cell extract.](http://images.novusbio.com/fullsize/CREB-Antibody-Western-Blot-NBP2-22516-img0001.jpg "Western Blot: CREB Antibody [NBP2-22516] - Analysis of GH4 cell extract.")
![Immunocytochemistry/Immunofluorescence: CREB Antibody [NBP2-22516] - Analysis of CREB (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22516-img0004.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody [NBP2-22516] - Analysis showing staining in the nucleus of human glioma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-Paraffin-NBP2-22516-img0007.jpg)
![Chromatin Immunoprecipitation: CREB Antibody [NBP2-22516] - Analysis performed using cross-linked chromatin from colon carcinoma cells treated with serum. IP was performed using a multiplex microplate Matrix ChIP assay of a CREB polyclonal antibody. Chromatin aliquots from cells were used per ChIP pull-down. Quantitative PCR data one in quadruplicate using 1ul of eluted DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of Egr1 or exon-1 or exon-2 of Egr1.Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM. A schematic representation of the Egr-1 locus is shown; xes represent exons (black boxes = translated regions, white boxes = untranslated regions), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/CREB-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-22516-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CREB Antibody [NBP2-22516] - Analysis of CREB (green) showing staining in the nucleus of SK-N-MC cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-22516-img0003.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody [NBP2-22516] - Analysis showing staining in the nucleus of mouse brain tissue (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-Paraffin-NBP2-22516-img0005.jpg)
![Immunohistochemistry-Paraffin: CREB Antibody [NBP2-22516] - Analysis showing staining in the nucleus of human lung adenocarcinoma (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/CREB-Antibody-Immunohistochemistry-Paraffin-NBP2-22516-img0006.jpg)
![Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the HRP conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 overnight at 4?C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Western-Blot-NBP2-37822-img0011.jpg "Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the HRP conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRP-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 overnight at 4?C on a rocking platform and washed in TBS-0.1% Tween-20. Chemiluminescent detection was performed using SuperSignal West Pico.")
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 680 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Immunocytochemistry-Immunofluorescence-NBP2-37822-img0009.jpg)
![Flow Cytometry: c-Myc Antibody (Myc.A7) [NBP2-37822] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (Myc.A7) NBP2-37822AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Flow-Cytometry-NBP2-37822-img0013.jpg)
![Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis of 1:1000 (1ug/mL) Ab dilution probed against HEK293 cells transfected with Myc-tagged protein vector; untransfected (1) and transfected (2)](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Western-Blot-NBP2-37822-img0005.jpg)
![Western Blot: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 680 conjugate of NBP2-37822. Detection of Myc Epitope Tag was performed by loading various amounts of E. coli lysate containing a multi-epitope tagged protein per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a DyLight 680-conjugated Myc Epitope Tag monoclonal antibody at a dilution of 1:1000 for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Detection was performed using the LI-COR Odyssey.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Western-Blot-NBP2-37822-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 488 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Immunocytochemistry-Immunofluorescence-NBP2-37822-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: c-Myc Antibody (Myc.A7) [NBP2-37822] - Analysis using the DyLight 550 conjugate of NBP2-37822. Staining of HeLa cells transfected with a construct containing a Myc Epitope Tag. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Immunocytochemistry-Immunofluorescence-NBP2-37822-img0008.jpg)
![Flow Cytometry: c-Myc Antibody (Myc.A7) [NBP2-37822] - An intracellular stain was performed on HepG2 cells with c-Myc Antibody (Myc.A7) NBP2-37822AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.](http://images.novusbio.com/fullsize/c-Myc-Antibody-Myc-A7-Flow-Cytometry-NBP2-37822-img0012.jpg)
![Western Blot: STAT3 Antibody (OTI21E7) [NBP1-48014] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells and STAT3-Knockout Hela cells were separated by SDS-PAGE and immunoblotted with anti-STAT3 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody as a loading control.(1:500)](http://images.novusbio.com/fullsize/STAT3-Antibody-OTI21E7-Western-Blot-NBP1-48014-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: STAT3 Antibody (OTI21E7) [NBP1-48014] - Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells and STAT3-Knockout Hela cells were separated by SDS-PAGE and immunoblotted with anti-STAT3 monoclonal antibody. Then the blotted membrane was stripped and reprobed with anti-b-actin antibody as a loading control.(1:500)")
![<b>Genetic Strategies Validation. </b>Western Blot: STAT3 Antibody (OTI21E7) [NBP1-48014] - Analysis of extracts (35ug) from different cell line by using anti-STAT3 monoclonal antibody. (1:500)](http://images.novusbio.com/fullsize/STAT3-Antibody-OTI21E7-Western-Blot-NBP1-48014-img0011.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] Staining of paraffin-embedded thyroid using anti-STAT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0009.jpg)
![Western Blot: STAT3 Antibody (21E7) [NBP1-48014] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY STAT3(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT3.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Western-Blot-NBP1-48014-img0001.jpg)
![Western Blot: STAT3 Antibody (OTI21E7) [NBP1-48014] - Analysis of extracts (35ug) from different cell line by using anti-STAT3 monoclonal antibody. (1:500)](http://images.novusbio.com/fullsize/STAT3-Antibody-OTI21E7-Western-Blot-NBP1-48014-img0010.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded breast using anti-STAT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0002.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded Carcinoma of kidney using anti-STAT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0003.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded Carcinoma of lung using anti-STAT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0004.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded Carcinoma of pancreas using anti-STAT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0005.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded colon using anti-STAT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0006.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded kidney using anti-STAT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0007.jpg)
![Immunohistochemistry-Paraffin: STAT3 Antibody (21E7) [NBP1-48014] - Staining of paraffin-embedded lung using anti-STAT3 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/STAT3-Antibody-21E7-Immunohistochemistry-Paraffin-NBP1-48014-img0008.jpg)