| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-04320SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52716-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-24965SS | |||
| Azide and BSA Free | NBP2-80633 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29623SS | |||
| Azide and BSA Free | NBP2-80957 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33134 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-37258SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44857 | |||
| Azide and BSA Free | NBP2-34528-0.1mg | |||
![Western Blot: Nanog Antibody (5A10) [NBP1-04320] - The cell lysates of NIH3T3 (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Western-Blot-NBP1-04320-img0001.jpg "Western Blot: Nanog Antibody (5A10) [NBP1-04320] - The cell lysates of NIH3T3 (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunohistochemistry: Nanog Antibody (5A10) [NBP1-04320] - Nuclear expression of NANOG [(C), red] was demonstrated on the endothelium of the microvessels which expressed CD34 [(C), green] within stroma. The NANOG+ cells [(C), red] that do not express CD34 were seen within the tumor nests and the stroma. Image collected and cropped by CiteAb from the following publication (journal.frontiersin.org/article/10.3389/fsurg.2016.00046), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Immunohistochemistry-NBP1-04320-img0006.jpg)
![Flow Cytometry: Nanog Antibody (5A10) [NBP1-04320] - Flow cytometry analysis of Nanog in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Flow-Cytometry-NBP1-04320-img0003.jpg)
![Immunohistochemistry-Paraffin: Nanog Antibody (5A10) [NBP1-04320] - Human seminoma tissue were incubated with anti-human Nanog (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodiumcitrate buffer and detected using Diaminobenzidine (DAB)](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Immunohistochemistry-Paraffin-NBP1-04320-img0004.jpg)
![Immunohistochemistry: Nanog Antibody (5A10) [NBP1-04320] - NANOG was seen in cells within the tumor nests [(F), brown, thick arrows] and the stroma [(F), brown, arrowheads], and the endothelium of the microvessels within the stroma [(F), brown, thin arrows]. Image collected and cropped by CiteAb from the following publication (journal.frontiersin.org/article/10.3389/fsurg.2016.00046), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nanog-Antibody-5A10-Immunohistochemistry-NBP1-04320-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Nanog Antibody (5A10) - BSA Free [NBP1-04320] - Representative IF IHC-stained sections of MDBMSCC demonstrating the expression of pSTAT3 [(A), red] & EMA [(A), green] by cells within the tumor nests. There was a CSC subpopulation remonstrating nuclear co-expression of STAT3 [(B), red] & CD34 [(B), green], appearing as orange, on the endothelium of the microvessels within the stroma; & another subpopulation staining only positively for pSTAT3 within the stroma [(B), red]. Nuclear expression of NANOG [(C), red] was demonstrated on the endothelium of the microvessels which expressed CD34 [(C), green] within stroma. The NANOG+ cells [(C), red] that do not express CD34 were seen within the tumor nests & the stroma. SOX2 [(D), red] was also expressed by cells within tumor nests & the stroma, & the endothelium of the microvessels expressing CD34 [(D), green]. Nuclear expression of both SOX2 [(E), red] & SALL4 [(E), green], appearing as orange, was seen on the cells within the tumor nests & the stroma. Expression of both SOX2 [(F), red] & OCT4 [(F), green], appearing as orange, was seen on cells within the tumor nests & the stroma, & the endothelium of the microvessels within the stroma. pSTAT3 [(G), red] & membranous staining CD44 [(G), green] were co-expressed by cells within the tumor nests. Scale bars: 20 μm. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/Article/10.3389/fsurg.2016.00046/abstract), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-04320_mouse-nanog-mab-5a10-bsa-free-310202416165642.jpg)
![Western Blot: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Western blot analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Western-Blot-NBP2-37597-img0003.jpg "Western Blot: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Western blot analysis using OTX2 mouse mAb against HepG2 (1), Jurkat (2), and NTERA-2 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Otx2 Antibody (1H12C4B5) [NBP2-37597] - ARPE-19 monolayer on glass substrate. Fixative 4% PFA Blocking Goat Serum Dilution 1:200. Incubation 1h @RT. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Immunocytochemistry-NBP2-37597-img0008.jpg)
![Immunohistochemistry-Paraffin: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Analysis of paraffin-embedded prostate tissues (left) and colon cancer tissues (right) using OTX2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Immunohistochemistry-Paraffin-NBP2-37597-img0007.jpg)
![Flow Cytometry: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Flow cytometric analysis of HepG2 cells using OTX2 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Flow-Cytometry-NBP2-37597-img0005.jpg)
![Western Blot: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Western blot analysis using OTX2 mAb against human OTX2 (AA: 40-297) recombinant protein. (Expected MW is 65 kDa)](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Western-Blot-NBP2-37597-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Immunofluorescence analysis of HepG2 cells using OTX2 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-Immunocytochemistry-Immunofluorescence-NBP2-37597-img0004.jpg)
![ELISA: Otx2 Antibody (1H12C4B5) [NBP2-37597] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Otx2-Antibody-1H12C4B5-ELISA-NBP2-37597-img0001.jpg)
![Western Blot: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Analysis of Tau (p Ser396) on SHSY5Y cell lysates. Lane 1: SHSY5Y cells, whole cell lysate, 10ug/lane Lane 2: SHSY5Y cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Tau (p Ser396) antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 79 kDa Observed band size: 70/130 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 2 minutes 34 seconds](http://images.novusbio.com/fullsize/Tau-p-Ser396-Antibody-SN62-09-Western-Blot-NBP2-67702-img0006.jpg "Western Blot: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Analysis of Tau (p Ser396) on SHSY5Y cell lysates. Lane 1: SHSY5Y cells, whole cell lysate, 10ug/lane Lane 2: SHSY5Y cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes : Anti-Tau (p Ser396) antibody at 1/500 dilution. Anti-GAPDH antibody at 1/10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 79 kDa Observed band size: 70/130 kDa Blocking and diluting buffer: 5% BSA. Exposure time: 2 minutes 34 seconds")
![Immunocytochemistry/Immunofluorescence: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Staining phospho -Tau(S396) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Tau-p-Ser396-Antibody-SN62-09-Immunocytochemistry-Immunofluorescence-NBP2-67702-img0004.jpg)
![Immunohistochemistry-Paraffin: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-Tau [p Ser396] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tau-p-Ser396-Antibody-SN62-09-Immunohistochemistry-Paraffin-NBP2-67702-img0008.jpg)
![Western Blot: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Western blot analysis of Tau on SHSY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rab](http://images.novusbio.com/fullsize/Tau-p-Ser396-Antibody-SN62-09-Western-Blot-NBP2-67702-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Staining phospho -Tau(S396) in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Tau-p-Ser396-Antibody-SN62-09-Immunocytochemistry-Immunofluorescence-NBP2-67702-img0001.jpg)
![Immunohistochemistry-Paraffin: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Analysis of paraffin-embedded mouse brain tissue using anti- phospho -Tau(S396) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tau-p-Ser396-Antibody-SN62-09-Immunohistochemistry-Paraffin-NBP2-67702-img0002.jpg)
![Immunohistochemistry-Paraffin: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Analysis of paraffin-embedded rat brain tissue using anti- phospho -Tau(S396) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tau-p-Ser396-Antibody-SN62-09-Immunohistochemistry-Paraffin-NBP2-67702-img0003.jpg)
![Immunohistochemistry-Paraffin: Tau [p Ser396] Antibody (SN62-09) [NBP2-67702] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Tau [p Ser396] antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tau-p-Ser396-Antibody-SN62-09-Immunohistochemistry-Paraffin-NBP2-67702-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Bax Antibody [NBP2-67285] - Staining Bax in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-67285-img0007.jpg "Immunocytochemistry/Immunofluorescence: Bax Antibody [NBP2-67285] - Staining Bax in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Bax Antibody [NBP2-67285] - Analysis of paraffin-embedded mouse testis tissue using anti-Bax antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP2-67285-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Bax Antibody [NBP2-67285] - Staining Bax in HepG2 cells (green). The nuclear counter stain is DAPI (blue).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-67285-img0002.jpg)
![Immunohistochemistry-Paraffin: Bax Antibody [NBP2-67285] - Analysis of paraffin-embedded human cervical tissue using anti-Bax antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP2-67285-img0003.jpg)
![Immunohistochemistry-Paraffin: Bax Antibody [NBP2-67285] - Analysis of paraffin-embedded human gall bladder tissue using anti-Bax antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bax-Antibody-Immunohistochemistry-Paraffin-NBP2-67285-img0004.jpg)
![Western Blot: Bax Antibody [NBP2-67285] - Analysis of BAX on different lysates with Rabbit anti-BAX antibody at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution.Lane 1: HeLa cell lysateLane 2: MCF7 cell lysateLane 3: HEK-293 cell lysateLysates/proteins at 15 ug/Lane.Predicted band size: 21 kDaObserved band size: 21 kDaExposure time: 1 minute 34 seconds; ECL: K1801;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67285_rabbit-monoclonal-bax-antibody-western-blot-1872024115652..jpg)
![Immunocytochemistry/Immunofluorescence: Bax Antibody [NBP2-67285] - Analysis of SHSY5Y cells labeling BAX with Rabbit anti-BAX antibody at 1/200 dilution.Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-BAX antibody at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488,) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.Beta â…¢ tubulin ( red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-67285_rabbit-monoclonal-bax-antibody-immunocytochemistry-immunofluorescence-1872024115923..jpg)
![Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Cell surface analysis of CD19 in BALB/c mouse splenocytes using 1 ug of this antibody. Green: isotype control; red: anti-CD19 PE. Image using the PE form of this antibody.](http://images.novusbio.com/fullsize/CD19-Antibody-1D3-Flow-Cytometry-NBP2-24965-img0011.jpg "Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Cell surface analysis of CD19 in BALB/c mouse splenocytes using 1 ug of this antibody. Green: isotype control; red: anti-CD19 PE. Image using the PE form of this antibody.")
![Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Cell surface analysis of CD19 in mouse splenocytes, 25 ug/10^6 cells. Anti-rat PE secondary and cell surface staining kit were used in this test.](http://images.novusbio.com/fullsize/CD19-Antibody-1D3-Flow-Cytometry-NBP2-24965-img0001.jpg)
![Flow (Cell Surface): CD19 Antibody (1D3) [NBP2-24965] - Analysis using the Allophycocyanin conjugate of NBP2-24965. Staining of CD19 in BALB/c mouse splenocytes using 1 ug of this antibody. Green represents isotype control; red represents anti-CD19 APC.](http://images.novusbio.com/fullsize/CD19-Antibody-1D3-Flow-Cell-Surface-NBP2-24965-img0006.jpg)
![Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Analysis using the Allophycocyanin conjugate of NBP2-24965. Staining of CD19 in BALB/c mouse splenocytes. Cells were stained with anti-CD4 PE this antibody and either 1 ug of isotype control antibody (left) or 1 ug of anti-CD19 APC (right).](http://images.novusbio.com/fullsize/CD19-Antibody-1D3-Flow-Cytometry-NBP2-24965-img0007.jpg)
![Flow Cytometry: CD19 Antibody (1D3) [NBP2-24965] - Analysis using the PE conjugate of NBP2-24965. Staining of CD19 in BALB/c mouse splenocytes. Cells were stained with anti-CD4 FITC this antibody and either 1 ug of isotype control antibody (left, ) or 1 ug of anti-CD19 PE (right).](http://images.novusbio.com/fullsize/CD19-Antibody-1D3-Flow-Cytometry-NBP2-24965-img0010.jpg)
![Western Blot: SOX2 Antibody (4G8) [NBP2-29623] - Western blot detection of SOX2 in A. HEK293 cell lysate B. NCCIT cell lysate and C. Full length recombinant protein.](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Western-Blot-NBP2-29623-img0001.jpg "Western Blot: SOX2 Antibody (4G8) [NBP2-29623] - Western blot detection of SOX2 in A. HEK293 cell lysate B. NCCIT cell lysate and C. Full length recombinant protein.")
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human lung cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a punctate immunopositivity of SOX2 protein in the cytoplasm and the staining was mainly localized in the perinuclear region of the cells [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0013.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC-P analysis of SOX2 protein in a section of human kidney using 5 ug/ml concentration of SOX2 antibody (clone 4G8). The representative image shows distinct nuclear and cytoplasmic staining pattern in cells of different types of renal tubules and blood vessels in the kidney section [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0002.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC-P analysis of SOX2 protein in a section of human renal cell carcinoma using 5 ug/ml concentration of SOX2 antibody (clone 4G8). Granular nuclear and cytoplasmic staining pattern was observed in the carcinoma cells, whereas, a weak to negligible staining was observed in the stroma areas [Magnification 40X]..](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0003.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human liver using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The liver section depicted a diffused cytoplasmic staining in the hepatocytes [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0004.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human brain using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The representative photomicrograph depicts a cytoplasmic and nuclear immunopositivity of SOX2 protein in brain [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0006.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded section of normal human colon tissue using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. A weak and diffused cytoplasmic staining was observed in majority of the cells in mucosal layer of colon [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0007.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human bladder cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a cytoplasmic and nuclear immunepositivity for SOX2 protein [Magnification 10X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0008.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a cytoplasmic and nuclear immunepositivity, whereas the stroma was largely negative for SOX2 protein [Magnification 10X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0009.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human uterine cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a punctate staining in the cytoplasm and nuclei, whereas the stroma was very weakly positive for SOX2 protein [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0010.jpg)
![Western Blot: G3BP1 Antibody [NBP1-18922] - Whole cell lysate (50 ug) from HeLa, MCF-7, U2OS, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G3BP1antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 1 second.](http://images.novusbio.com/fullsize/G3BP1-Antibody-Western-Blot-NBP1-18922-img0008.jpg "Western Blot: G3BP1 Antibody [NBP1-18922] - Whole cell lysate (50 ug) from HeLa, MCF-7, U2OS, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-G3BP1antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with anexposure time of 1 second.")
![Immunocytochemistry/Immunofluorescence: G3BP1 Antibody [NBP1-18922] - G3BP1 in red (Alexa fluor 555) and nucleus in blue (DAPI). Human primary fibroblasts untreated in the left and treated in the right with G3BP1 stress granules labeling. Alexa fluor 555 1s exposure and DAPI 1s exposure. Fixation: PFA 4% 10min RT, permeabilization: Triton 0.1% TBS, blocking: Donkey Serum 10% TBS-T, Ab dilution: 1:1000, Secondary Ab: Alexa fluor 555 Donkey anti-rabbit 1:600, Mounting medium with DAPI. Image submitted by a verified customer review.](http://images.novusbio.com/fullsize/G3BP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-18922-img0007.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: G3BP1 Antibody [NBP1-18922] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50mcg) cells. Affinity purified rabbit anti-G3BP1 antibody used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. G3BP1 was also immunoprecipitated by rabbit anti-G3BP1 antibody NBP1-18923, which recognizes a downstream epitope.](http://images.novusbio.com/fullsize/G3BP1-Antibody-Western-Blot-NBP1-18922-img0006.jpg)
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMC2 antibody NB100-373 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: SMC2 Antibody [NB100-373] - SMC2 in HaCaT keratinocytes treated with control shRNA (upper panels) and treated with Nesprin-2-SMC domain specific shRNA (lower panels). Nesprin-2 was detected with mAb K20-478. Bar, 10 um. Image collected and cropped by CiteAb from the following publication (//www.hindawi.com/journals/ijcb/2017/8607532/), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SMC2-Antibody-Immunocytochemistry-Immunofluorescence-NB100-373-img0015.jpg)
![Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 on HeLa whole cell lysate using NB100-373.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: SMC2 Antibody [NB100-373] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibod ies : Affinity purified rabbit anti-SMC2 antibody (lot was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.](http://images.novusbio.com/fullsize/SMC2-Antibody-Western-Blot-NB100-373-img0012.jpg)
![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - Western Blot Analysis of human Prostate Cancer PC-3 cell lysate Cytokeratin 19 Antibody (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Western-Blot-NBP2-15186-img0011.jpg "Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - Western Blot Analysis of human Prostate Cancer PC-3 cell lysate Cytokeratin 19 Antibody (A53-B/A2.26).")
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - Immunofluorescence Analysis of MCF-7 cells labeling Cytokeratin 19 with Cytokeratin 19 Antibody (A53-B/A2.26) followed by Goat anti-Mouse IgG-CF488 (Cyan). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Immunocytochemistry-Immunofluorescence-NBP2-15186-img0009.jpg)
![Immunohistochemistry-Frozen: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - Breast cancer tissue stained for Cytokeratin 19 (red) and counter stained with DAPI (blue). IHC-Fr image submitted by a verified customer review. Image using the Janelia Fluor 549, Azide and BSA free format of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Immunohistochemistry-Frozen-NBP2-15186-img0013.jpg)
![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - analysis of Keratin-19 in 1) HepG2 and 2) MCF7 lysate using of Keratin-19 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Western-Blot-NBP2-15186-img0003.jpg)
![Western Blot: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - Western blot 1) Hep-G2 and 2) MCF-7 lysate using of Cytokeratin 19 Antibody (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Western-Blot-NBP2-15186-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-Cytokeratin 19 (A53-B/A2.26) conjugated to Alexa Fluor 488 [NBP2-33134AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the Alexa Fluor 488 version of this antibody.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Immunocytochemistry-Immunofluorescence-NBP2-15186-img0008.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - Formalin-paraffin colon carcinoma stained with Cytokeratin 19 Ab (A53-B/A2.26).](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Immunohistochemistry-Paraffin-NBP2-15186-img0005.jpg)
![Simple Western: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - Simple Western lane view shows a specific band for Cytokeratin 19 in 0.2 mg/ml of HeLa lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Simple-Western-NBP2-15186-img0006.jpg)
![Simple Western: Cytokeratin 19 Antibody (A53-B/A2.26) [NBP2-15186] - Electropherogram image of the corresponding Simple Western lane. Cytokeratin 19 antibody was used at 10 ug/ml dilution of HeLa lysates(s) respectively.](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-A53-B-A2-26-Simple-Western-NBP2-15186-img0007.jpg)
![Western Blot: SLIRP Antibody [NB110-37258] - Detection of SLIRP protein in a human liver. Primary was used at 0.6 ug/ml.](http://images.novusbio.com/fullsize/SLIRP-Antibody-Western-Blot-NB110-37258-img0005.jpg "Western Blot: SLIRP Antibody [NB110-37258] - Detection of SLIRP protein in a human liver. Primary was used at 0.6 ug/ml.")
![Immunocytochemistry/Immunofluorescence: SLIRP Antibody [NB110-37258] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-SLIRP (NB110-37258) at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/SLIRP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-37258-img0009.jpg)
![Immunohistochemistry: SLIRP Antibody [NB110-37258] - Analysis of SLIRP in mouse intestine using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/SLIRP-Antibody-Immunohistochemistry-NB110-37258-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: SLIRP Antibody [NB110-37258] - SLIRP antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/SLIRP-Antibody-Immunocytochemistry-Immunofluorescence-NB110-37258-img0006.jpg)
![Simple Western: SLIRP Antibody [NB110-37258] - Simple Western lane view shows a specific band for SLIRP in 0.2 mg/ml of Human Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize/SLIRP-Antibody-Simple-Western-NB110-37258-img0008.jpg)
![Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD45 antibody (2B11 + PD7/26) [NBP2-34287].](http://images.novusbio.com/fullsize/CD45-Antibody-2B11-+-PD7-26-Immunohistochemistry-Paraffin-NBP2-34287-img0003.jpg "Formalin-fixed, paraffin-embedded human tonsil (20X) stained with CD45 antibody (2B11 + PD7/26) [NBP2-34287].")
![Western Blot Analysis of human Spleen tissue lysates using CD45 antibody (2B11 + PD7/26) [NBP2-34287]. Observed molecular weight ~200 kDa.](http://images.novusbio.com/fullsize/CD45-Antibody-2B11-+-PD7-26-Western-Blot-NBP2-34287-img0007.jpg)
![Formalin-fixed, paraffin-embedded human tonsil (10X) stained with CD45 antibody(2B11 + PD7/26) [NBP2-34287].](http://images.novusbio.com/fullsize/CD45-Antibody-2B11-+-PD7-26-Immunohistochemistry-Paraffin-NBP2-34287-img0001.jpg)
![Flow Cytometric Analysis of PFA-fixed Jurkat cells using CD45 antibody (2B11 + PD7/26) [NBP2-34287] followed by Goat anti-Mouse-IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD45-Antibody-2B11-+-PD7-26-Flow-Cytometry-NBP2-34287-img0006.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (2B11 + PD7/26) [NBP2-34287] - Staining of human tonsil with Alexa Fluor 488 conjugate of CD45 Antibody (2B11 + PD7/26), pH 9 antigen retrieval. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-34287_mouse-monoclonal-cd45-antibody-2b11-pd7-26-17720241003243.jpg)