| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52664-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37358-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61840-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61795-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90058-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90112-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89744-50ul | |||
![Flow Cytometry: CD19 Antibody (4G7) - Chimeric [NBP2-52664] - Flow-cytometry using anti-CD19 antibody 4G7. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of 4G7 (NBP2-52664, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD19-Antibody-4G7-Chimeric-Flow-Cytometry-NBP2-52664-img0001.jpg "Flow Cytometry: CD19 Antibody (4G7) - Chimeric [NBP2-52664] - Flow-cytometry using anti-CD19 antibody 4G7. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of 4G7 (NBP2-52664, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![<b>Biological Strategies Validation. </b>Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Western Blot: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Western blot analysis using ALCAM mAb against HEK293 (1) and ALCAM (AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ALCAM-CD166-Antibody-8E12C7-Western-Blot-NBP2-37358-img0003.jpg "Western Blot: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Western blot analysis using ALCAM mAb against HEK293 (1) and ALCAM (AA: 48-216)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Immunofluorescence analysis of Hela cells using ALCAM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/ALCAM-CD166-Antibody-8E12C7-Immunocytochemistry-Immunofluorescence-NBP2-37358-img0004.jpg)
![Immunohistochemistry: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using ALCAM mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ALCAM-CD166-Antibody-8E12C7-Immunohistochemistry-NBP2-37358-img0007.jpg)
![Flow Cytometry: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Flow cytometric analysis of JURKAT cells using ALCAM mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/ALCAM-CD166-Antibody-8E12C7-Flow-Cytometry-NBP2-37358-img0005.jpg)
![Western Blot: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Western blot analysis using ALCAM mAb against human ALCAM recombinant protein. (Expected MW is 44.9 kDa)](http://images.novusbio.com/fullsize/ALCAM-CD166-Antibody-8E12C7-Western-Blot-NBP2-37358-img0002.jpg)
![Immunohistochemistry: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ALCAM mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/ALCAM-CD166-Antibody-8E12C7-Immunohistochemistry-NBP2-37358-img0006.jpg)
![ELISA: ALCAM/CD166 Antibody (8E12C7) [NBP2-37358] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/ALCAM-CD166-Antibody-8E12C7-ELISA-NBP2-37358-img0001.jpg)
![Western Blot: Ran Antibody (8D1H12) [NBP2-61840] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-Western-Blot-NBP2-61840-img0006.jpg "Western Blot: Ran Antibody (8D1H12) [NBP2-61840] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: Ran Antibody (8D1H12) [NBP2-61840] - Analysis of paraffin-embedded rectum cancer tissues using RAN mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-Immunohistochemistry-NBP2-61840-img0004.jpg)
![Western Blot: Ran Antibody (8D1H12) [NBP2-61840] - Analysis using RAN mAb against human RAN (AA: 1-216) recombinant protein. (Expected MW is 50.4 kDa)](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-Western-Blot-NBP2-61840-img0005.jpg)
![Western Blot: Ran Antibody (8D1H12) [NBP2-61840] - Analysis using RAN mouse mAb against A431 (1), C6 (2), Jurkat (3), Hela (4), COS7 (5), and Jurkat (6) cell lysate.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-Western-Blot-NBP2-61840-img0007.jpg)
![Immunohistochemistry: Ran Antibody (8D1H12) [NBP2-61840] - Analysis of paraffin-embedded esophageal cancer tissues using RAN mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-Immunohistochemistry-NBP2-61840-img0003.jpg)
![ELISA: Ran Antibody (8D1H12) [NBP2-61840] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-ELISA-NBP2-61840-img0001.jpg)
![ELISA: Ran Antibody (8D1H12) [NBP2-61840] - Analysis of Hela cells using RAN mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Ran-Antibody-8D1H12-ELISA-NBP2-61840-img0002.jpg)
![Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis using IDH1 mAb against HEK293 (1) and IDH1 (AA: 156-298)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-7G8A1-Western-Blot-NBP2-61795-img0005.jpg "Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis using IDH1 mAb against HEK293 (1) and IDH1 (AA: 156-298)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis of Hela cells using IDH1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-7G8A1-Immunocytochemistry-Immunofluorescence-NBP2-61795-img0003.jpg)
![Flow Cytometry: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis of Hela cells using IDH1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-7G8A1-Flow-Cytometry-NBP2-61795-img0002.jpg)
![Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis using IDH1 mAb against human IDH1 (AA: 156-298) recombinant protein. (Expected MW is 41.8 kDa)](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-7G8A1-Western-Blot-NBP2-61795-img0004.jpg)
![Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Analysis using IDH1 mouse mAb against HepG2 (1), NIH/3T3 (2), C2C12 (3), COS7 (4), and SW480 (5) cell lysate.](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-7G8A1-Western-Blot-NBP2-61795-img0006.jpg)
![ELISA: Isocitrate Dehydrogenase 1/IDH1 Antibody (7G8A1) [NBP2-61795] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Isocitrate-Dehydrogenase-1-IDH1-Antibody-7G8A1-ELISA-NBP2-61795-img0001.jpg)
![Western Blot: CD38 Antibody (PD01-49) [NBP3-32151] - Western blot analysis of CD38 on Daudi cell lysates with Rabbit anti-CD38 antibody (NBP3-32151) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 34 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32151) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-278202416285274.jpg "Western Blot: CD38 Antibody (PD01-49) [NBP3-32151] - Western blot analysis of CD38 on Daudi cell lysates with Rabbit anti-CD38 antibody (NBP3-32151) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 34 kDa Observed band size: 45 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32151) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD38 Antibody (PD01-49) [NBP3-32151] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD38 antibody (NBP3-32151) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32151) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-29820241435231.jpg)
![Immunocytochemistry/ Immunofluorescence: CD38 Antibody (PD01-49) [NBP3-32151] - Immunocytochemistry analysis of A549 cells labeling CD38 with Rabbit anti-CD38 antibody (NBP3-32151) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD38 antibody (NBP3-32151) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-2920241192330.jpg)
![Flow Cytometry: CD38 Antibody (PD01-49) [NBP3-32151] - Flow cytometric analysis of THP-1 cells labeling CD38. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32151, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32151_rabbit-cd38-mab-pd01-49-29202416453252.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human lymph node with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0004.jpg "Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human lymph node with rabbit monoclonal antibody at 1:200 dilution.")
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human breast carcinoma with rabbit monoclonal antibody at 1:200).](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0001.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human liver with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0002.jpg)
![Immunohistochemistry-Paraffin: PCNA Antibody (144) [NBP2-90058] - Staining of human PCNA in human colon carcinoma with rabbit monoclonal antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/PCNA-Antibody-144-Immunohistochemistry-Paraffin-NBP2-90058-img0003.jpg)
![Western Blot: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Western blot analysis of Complement C5 on different lysates with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (40 ug/Lane) Predicted band size: 188 kDa Observed band size: 188/72 kDa Exposure time: 43 seconds; ECL 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32209) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-278202415295515.jpg "Western Blot: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Western blot analysis of Complement C5 on different lysates with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (40 ug/Lane) Predicted band size: 188 kDa Observed band size: 188/72 kDa Exposure time: 43 seconds; ECL 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32209) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32209) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-29820241435282.jpg)
![Immunocytochemistry/ Immunofluorescence: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Immunocytochemistry analysis of HepG2 cells labeling Complement C5 with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-2920241192373.jpg)
![Flow Cytometry: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Flow cytometric analysis of HepG2 cells labeling Complement C5. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32209, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-29202416372127.jpg)
![Flow Cytometry: CD59 Antibody (029) [NBP2-90112] - Analysis of Human CD59 expression on human whole blood lymphocytes. Cells were stained with purified anti-Human CD59, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.](http://images.novusbio.com/fullsize/CD59-Antibody-029-Flow-Cytometry-NBP2-90112-img0001.jpg "Flow Cytometry: CD59 Antibody (029) [NBP2-90112] - Analysis of Human CD59 expression on human whole blood lymphocytes. Cells were stained with purified anti-Human CD59, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.")
![Western Blot: TCF21 Antibody [NBP2-83632] - Host: Rabbit. Target Name: TCF21. Sample Tissue: Human Jurkat Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/TCF21-Antibody-Western-Blot-NBP2-83632-img0007.jpg "Western Blot: TCF21 Antibody [NBP2-83632] - Host: Rabbit. Target Name: TCF21. Sample Tissue: Human Jurkat Whole Cell. Antibody Dilution: 1ug/ml")
![Immunohistochemistry: TCF21 Antibody [NBP2-83632] - Immunohistochemistry with Human Brain, cortex, senile plaque tissue at an antibody concentration of 5.0ug/ml using anti-TCF21 antibody](http://images.novusbio.com/fullsize/TCF21-Antibody-Immunohistochemistry-NBP2-83632-img0001.jpg)
![Western Blot: TCF21 Antibody [NBP2-83632] - Host: Rabbit. Target: TCF21. Positive control (+): Human kidney (KI). Negative control (-): Human liver (LI). Antibody concentration: 1ug/ml](http://images.novusbio.com/fullsize/TCF21-Antibody-Western-Blot-NBP2-83632-img0002.jpg)
![Western Blot: TCF21 Antibody [NBP2-83632] - Host: Rabbit. Target Name: TCF21. Sample Tissue: Human Hela. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/TCF21-Antibody-Western-Blot-NBP2-83632-img0003.jpg)
![Western Blot: TCF21 Antibody [NBP2-83632] - Host: Rabbit. Target Name: TCF21. Sample Tissue: Human Ovary Tumor. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/TCF21-Antibody-Western-Blot-NBP2-83632-img0004.jpg)
![Western Blot: TCF21 Antibody [NBP2-83632] - Host: Rabbit. Target Name: TCF21. Sample Tissue: Human 293T Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/TCF21-Antibody-Western-Blot-NBP2-83632-img0005.jpg)
![Western Blot: TCF21 Antibody [NBP2-83632] - Host: Rabbit. Target Name: TCF21. Sample Tissue: Human Ovary Tumor. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/TCF21-Antibody-Western-Blot-NBP2-83632-img0006.jpg)
![Western Blot: TCF21 Antibody [NBP2-83632] - Host: Rabbit. Target Name: TCF21. Sample Tissue: Human HCT116 Whole Cell. Antibody Dilution: 3ug/ml](http://images.novusbio.com/fullsize/TCF21-Antibody-Western-Blot-NBP2-83632-img0008.jpg)
![Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Surface staining of human peripheral blood with anti-CD54 (1H4) FITC. Image using the FITC format of this antibody.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cell-Surface-NB110-58732-img0010.jpg "Flow (Cell Surface): ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Surface staining of human peripheral blood with anti-CD54 (1H4) FITC. Image using the FITC format of this antibody.")
![Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Expression of CD54 in normal human peripheral blood, as detected with anti-CD54 (1H4) biotin.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cytometry-NB110-58732-img0004.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Fig. 1. Surface staining of U937 human histiocytic lymphoma cell line with anti-human CD54 (1H4) PE. Total viable cells were used for analysis. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cytometry-NB110-58732-img0005.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Staining of human peripheral blood with anti-CD54 (1H4) APC.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cytometry-NB110-58732-img0007.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Staining of human peripheral blood with anti-CD54 (1H4) Alexa Fluor (R) 700.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cytometry-NB110-58732-img0009.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (1H4) - Azide Free [NB110-58732] - Surface staining of human peripheral blood with anti-CD54 (1H4) PerCP. Image from the PE version of this antibody.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-1H4-Azide-Free-Flow-Cytometry-NB110-58732-img0006.jpg)
![Flow Cytometry: TfR (Transferrin R) Antibody (040) [NBP2-89744] - Analysis of Human TFRC(CD71) expression on U937 cells. Cells were stained with purified anti-Human TFRC(CD71), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-040-Flow-Cytometry-NBP2-89744-img0002.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (040) [NBP2-89744] - Analysis of Human TFRC(CD71) expression on U937 cells. Cells were stained with purified anti-Human TFRC(CD71), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/Immunofluorescence: TfR (Transferrin R) Antibody (040) [NBP2-89744] - Staining of Human TFRC in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89744 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89744_rabbit-monoclonal-tfr-transferrin-r-antibody-040-235202514492378.png)