| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93296-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37387-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37337-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11536 | |||
![Western Blot: PHLPP Antibody [NBP2-93296] - Western blot analysis of extracts of various cell lines, using PHLPP antibody (NBP2-93296) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/PHLPP Antibody-Western Blot-NBP2-93296-img0003.jpg "Western Blot: PHLPP Antibody [NBP2-93296] - Western blot analysis of extracts of various cell lines, using PHLPP antibody (NBP2-93296) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: PHLPP Antibody [NBP2-93296] - Immunohistochemistry of paraffin-embedded mouse kidney using PHLPP Rabbit pAb (NBP2-93296) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PHLPP-Antibody-Immunohistochemistry-Paraffin-NBP2-93296-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: PHLPP Antibody - Azide and BSA Free [NBP2-93296] - Immunofluorescence analysis of U-2 OS cells using PHLPP Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93296_rabbit-polyclonal-phlpp-antibody-4220251429019.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mouse mAb against HL60 (1) and HepG2 (2) cell lysate.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-Western-Blot-NBP2-37387-img0004.jpg "Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mouse mAb against HL60 (1) and HepG2 (2) cell lysate.")
![Flow Cytometry: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Flow cytometric analysis of HepG2 cells using ALPI mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-Flow-Cytometry-NBP2-37387-img0005.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mAb against human ALPI recombinant protein. (Expected MW is 31.9 kDa)](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-Western-Blot-NBP2-37387-img0002.jpg)
![Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mAb against HEK293 (1) and ALPI (AA: 397-458)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-Western-Blot-NBP2-37387-img0003.jpg)
![ELISA: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Intestinal-Antibody-3B10E7-ELISA-NBP2-37387-img0001.jpg)
![Western Blot: TRACP/PAP/ACP5 Antibody (7E6A11) [NBP2-37337] - Western blot analysis using ACP5 mouse mAb against A431 (1), T47D (2), HepG2 (3), MOLT4 (4), Jurkat (5) and Hela (6) cell lysate.](http://images.novusbio.com/fullsize/TRACP-PAP-ACP5-Antibody-7E6A11-Western-Blot-NBP2-37337-img0004.jpg "Western Blot: TRACP/PAP/ACP5 Antibody (7E6A11) [NBP2-37337] - Western blot analysis using ACP5 mouse mAb against A431 (1), T47D (2), HepG2 (3), MOLT4 (4), Jurkat (5) and Hela (6) cell lysate.")
![Immunohistochemistry: TRACP/PAP/ACP5 Antibody (7E6A11) [NBP2-37337] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using ACP5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/TRACP-PAP-ACP5-Antibody-7E6A11-Immunohistochemistry-NBP2-37337-img0005.jpg)
![Western Blot: TRACP/PAP/ACP5 Antibody (7E6A11) [NBP2-37337] - Western blot analysis using ACP5 mAb against human ACP5 recombinant protein. (Expected MW is 37.3 kDa)](http://images.novusbio.com/fullsize/TRACP-PAP-ACP5-Antibody-7E6A11-Western-Blot-NBP2-37337-img0002.jpg)
![Western Blot: TRACP/PAP/ACP5 Antibody (7E6A11) [NBP2-37337] - Western blot analysis using ACP5 mAb against HEK293 (1) and ACP5 (AA: 221-325)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/TRACP-PAP-ACP5-Antibody-7E6A11-Western-Blot-NBP2-37337-img0003.jpg)
![ELISA: TRACP/PAP/ACP5 Antibody (7E6A11) [NBP2-37337] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/TRACP-PAP-ACP5-Antibody-7E6A11-ELISA-NBP2-37337-img0001.jpg)
![Immunohistochemistry: PTP alpha/PTPRA Antibody [NBP3-14454] - Immunohistochemistry of lung, non-small cell carcinoma,](http://images.novusbio.com/fullsize/PTP-alpha-PTPRA-Antibody-Immunohistochemistry-NBP3-14454-img0005.jpg "Immunohistochemistry: PTP alpha/PTPRA Antibody [NBP3-14454] - Immunohistochemistry of lung, non-small cell carcinoma,")
![Immunohistochemistry-Paraffin: PTP alpha/PTPRA Antibody [NBP3-14454] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skeletal muscle after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PTP-alpha-PTPRA-Antibody-Immunohistochemistry-Paraffin-NBP3-14454-img0001.jpg)
![Immunohistochemistry-Paraffin: PTP alpha/PTPRA Antibody [NBP3-14454] - Immunohistochemistry of formalin-fixed, paraffin-embedded human skin melanoma after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PTP-alpha-PTPRA-Antibody-Immunohistochemistry-Paraffin-NBP3-14454-img0002.jpg)
![Immunohistochemistry-Paraffin: PTP alpha/PTPRA Antibody [NBP3-14454] - Immunohistochemistry of formalin-fixed, paraffin-embedded human brain, cortex, after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/PTP-alpha-PTPRA-Antibody-Immunohistochemistry-Paraffin-NBP3-14454-img0003.jpg)
![Immunohistochemistry: PTP alpha/PTPRA Antibody [NBP3-14454] - Immunohistochemistry of breast carcinoma](http://images.novusbio.com/fullsize/PTP-alpha-PTPRA-Antibody-Immunohistochemistry-NBP3-14454-img0004.jpg)
![Immunohistochemistry: PTP alpha/PTPRA Antibody [NBP3-14454] - Immunohistochemistry of skin, malignant melanoma,](http://images.novusbio.com/fullsize/PTP alpha-PTPRA Antibody-Immunohistochemistry-NBP3-14454-img0006.jpg)
![Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-278202415295570.jpg "Western Blot: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Western blot analysis of Lamin A + C on different lysates with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: THP-1 cell lysate Lane 4: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 74/65 kDa Observed band size: 74/65 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32518) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32518) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29820241426267.jpg)
![Immunocytochemistry/ Immunofluorescence: Lamin A + C Antibody (A6F5-R) [NBP3-32518] - Immunocytochemistry analysis of HeLa cells labeling Lamin A + C with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Lamin A + C antibody (NBP3-32518) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32518_mouse-lamin-a-c-mab-a6f5-r-29202410484838.jpg)
![Western Blot: Rac1,2,3 Antibody [NBP3-32887] - Western blot analysis of Rac1,2,3 on different lysates with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 5 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32887) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-288202412442915.jpg "Western Blot: Rac1,2,3 Antibody [NBP3-32887] - Western blot analysis of Rac1,2,3 on different lysates with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 5 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32887) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Rac1,2,3 Antibody [NBP3-32887] - Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32887) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-29820241420957.jpg)
![Immunocytochemistry/ Immunofluorescence: Rac1,2,3 Antibody [NBP3-32887] - Immunocytochemistry analysis of SH-SY5Y cells labeling Rac1,2,3 with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-29202410234241.jpg)
![Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-288202412461629.jpg "Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PTEN Antibody (JE51-96) [NBP3-32873] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PTEN antibody (NBP3-32873) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32873) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29820241492644.jpg)
![Flow Cytometry: PTEN Antibody (JE51-96) [NBP3-32873] - Flow cytometric analysis of A431 cells labeling PTEN. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32873, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29202416372137.jpg)
![Western Blot: CDC2L2 Antibody [NB100-496] - Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDK11 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CDC2L2-Antibody-Western-Blot-NB100-496-img0009.jpg "Western Blot: CDC2L2 Antibody [NB100-496] - Whole cell lysate (15 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CDK11 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: CDC2L2 Antibody [NB100-496] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-CDK11 (NB100-496). Detection: DAB](http://images.novusbio.com/fullsize/CDC2L2-Antibody-Immunohistochemistry-NB100-496-img0006.jpg)
![Immunoprecipitation: CDC2L2 Antibody [NB100-496] - Detection of human CDK11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CDK11 antibody NB100-496 (lot NB100-496-3) used for IP at 3 ug per reaction. CDK11 was also immunoprecipitated by a previous lot of this antibody (lot NB100-496-2). For blotting immunoprecipitated CDK11, NB100-496 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/CDC2L2-Antibody-Immunoprecipitation-NB100-496-img0007.jpg)
![Western Blot: MTMR9 Antibody [NBP1-31078] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE MTMR9 antibody, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MTMR9-Antibody-Western-Blot-NBP1-31078-img0005.jpg "Western Blot: MTMR9 Antibody [NBP1-31078] - Sample (30 ug of whole cell lysate) A: Hep G2 7. 5% SDS PAGE MTMR9 antibody, antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: MTMR9 Antibody [NBP1-31078] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MTMR9-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31078-img0003.jpg)
![Immunohistochemistry-Paraffin: MTMR9 Antibody [NBP1-31078] - Paraffin-embedded mouse lung. MTMR9 antibody [C3], C-term diluted at 1:500.](http://images.novusbio.com/fullsize/MTMR9-Antibody-Immunohistochemistry-Paraffin-NBP1-31078-img0006.jpg)
![Western Blot: CDC25C Antibody (3E6) [NBP3-15178] - Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate. All lanes: CDC25C antibody at 1.65ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 52, 49, 46 KDa. Observed band size: 60 KDa.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Western-Blot-NBP3-15178-img0004.jpg "Western Blot: CDC25C Antibody (3E6) [NBP3-15178] - Positive WB detected in: Hela whole cell lysate, K562 whole cell lysate, Raji whole cell lysate, 293 whole cell lysate. All lanes: CDC25C antibody at 1.65ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 54, 52, 49, 46 KDa. Observed band size: 60 KDa.")
![Immunocytochemistry/Immunofluorescence: CDC25C Antibody (3E6) [NBP3-15178] - Immunofluorescence staining of Hela cells with at CDC25C antibody 1:55, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Immunocytochemistry-Immunofluorescence-NBP3-15178-img0001.jpg)
![Immunohistochemistry: CDC25C Antibody (3E6) [NBP3-15178] - IHC image of CDC25C antibody diluted at 1:165 and staining in paraffin-embedded human glioma cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Immunohistochemistry-NBP3-15178-img0003.jpg)
![Immunoprecipitation: CDC25C Antibody (3E6) [NBP3-15178] - Immunoprecipitating CDC25C in HEK293 whole cell lysate. Lane 1: Rabbit control IgG instead of CDC25C antibody in HEK293 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: CDC25C antibody (3ug) + HEK293 whole cell lysate (500ug). Lane 3: HEK293 whole cell lysate (20ug).](http://images.novusbio.com/fullsize/CDC25C-Antibody-3E6-Immunoprecipitation-NBP3-15178-img0002.jpg)
![Immunohistochemistry-Paraffin: TRACP/PAP/ACP5 Antibody (SPM601) [NBP2-45294] - Formalin-fixed, paraffin-embedded human Spleen stained with TRAcP Monoclonal Antibody (SPM601).](http://images.novusbio.com/fullsize/TRACP-PAP-ACP5-Antibody-SPM601-Immunohistochemistry-Paraffin-NBP2-45294-img0001.jpg "Immunohistochemistry-Paraffin: TRACP/PAP/ACP5 Antibody (SPM601) [NBP2-45294] - Formalin-fixed, paraffin-embedded human Spleen stained with TRAcP Monoclonal Antibody (SPM601).")