| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89621-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47774-0.1mg | |||
| IHC-Prediluted | NBP2-48224 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-20089SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84568-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33064 | |||
![Western Blot: JNK2 Antibody (011) [NBP2-89621] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:48 kDa Observed band size:54 kDa (We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Western-Blot-NBP2-89621-img0004.jpg "Western Blot: JNK2 Antibody (011) [NBP2-89621] - Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:48 kDa Observed band size:54 kDa (We are unsure as to the identity of these extra bands.)")
![Immunocytochemistry/Immunofluorescence: JNK2 Antibody (011) [NBP2-89621] - Staining of Human MAPK9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human MAPK9 monoclonal antibody (1:300) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Immunocytochemistry-Immunofluorescence-NBP2-89621-img0001.jpg)
![Immunohistochemistry-Paraffin: JNK2 Antibody (011) [NBP2-89621] - Staining of human MAPK9 in human brain with rabbit mnoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Immunohistochemistry-Paraffin-NBP2-89621-img0003.jpg)
![Immunohistochemistry-Paraffin: JNK2 Antibody (011) [NBP2-89621] - Staining of human MAPK9 in human kidney with rabbit monoclonal antibody (1:1000).](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Immunohistochemistry-Paraffin-NBP2-89621-img0002.jpg)
![Immunoprecipitation: JNK2 Antibody (011) [NBP2-89621] - Lane A:0.5 mg HepG2 Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg Jurkat Whole Cell Lysate Lane D:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-MAPK9 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-MAPK9 rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 44 kDa Observed band size: 44 kDa](http://images.novusbio.com/fullsize/JNK2-Antibody-011-Immunoprecipitation-NBP2-89621-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: JNK2 Antibody (011) [NBP2-89621] - Staining of Human MAPK9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89621 antibody (1:300) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/nbp2-89621_rabbit-monoclonal-jnk2-antibody-011-23520251544322.png)
![Western Blot: ULK3 Antibody (PSH01-09) [NBP3-33083] - Western blot analysis of ULK3 on different lysates with Rabbit anti-ULK3 antibody (NBP3-33083) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Mouse brain tissue lysate (40 ug/Lane) Lane 6: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33083) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33083_rabbit-ulk3-mab-psh01-09-28820241419159.jpg "Western Blot: ULK3 Antibody (PSH01-09) [NBP3-33083] - Western blot analysis of ULK3 on different lysates with Rabbit anti-ULK3 antibody (NBP3-33083) at 1/1,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: Jurkat cell lysate (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Lane 5: Mouse brain tissue lysate (40 ug/Lane) Lane 6: Rat brain tissue lysate (40 ug/Lane) Predicted band size: 53 kDa Observed band size: 53 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33083) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ULK3 Antibody (PSH01-09) [NBP3-33083] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-ULK3 antibody (NBP3-33083) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33083) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33083_rabbit-ulk3-mab-psh01-09-298202414262625.jpg)
![Immunohistochemistry: ULK3 Antibody (PSH01-09) [NBP3-33083] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling ULK3 with Rabbit anti-ULK3 antibody (NBP3-33083) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33083, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33083_rabbit-ulk3-mab-psh01-09-298202414161889.jpg)
![Immunocytochemistry/ Immunofluorescence: ULK3 Antibody (PSH01-09) [NBP3-33083] - Immunocytochemistry analysis of HeLa cells labeling ULK3 with Rabbit anti-ULK3 antibody (NBP3-33083) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ULK3 antibody (NBP3-33083) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33083_rabbit-ulk3-mab-psh01-09-29202410484852.jpg)
![Flow Cytometry: ULK3 Antibody (PSH01-09) [NBP3-33083] - Flow cytometric analysis of HeLa cells labeling ULK3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33083, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33083_rabbit-ulk3-mab-psh01-09-39202488469.jpg)
![Western Blot: Lyn Antibody (LYN-01) [NB500-519] - Fig. 1. Western Blotting analysis (non-reducing conditions) of Lyn using anti-Lyn (LYN-01). Lane 1: negative control Lane 2: RBL rat basophilic leukemia cell line Lane 3: JURKAT human peripheral blood T cell leukemia cell line Lane 4: A-431 human epidermoid carcinoma cell line Lane 5: U-937 human histiocytic lymphoma cell line.](http://images.novusbio.com/fullsize/Lyn-Antibody-LYN-01-Western-Blot-NB500-519-img0001.jpg "Western Blot: Lyn Antibody (LYN-01) [NB500-519] - Fig. 1. Western Blotting analysis (non-reducing conditions) of Lyn using anti-Lyn (LYN-01). Lane 1: negative control Lane 2: RBL rat basophilic leukemia cell line Lane 3: JURKAT human peripheral blood T cell leukemia cell line Lane 4: A-431 human epidermoid carcinoma cell line Lane 5: U-937 human histiocytic lymphoma cell line.")
![Western Blot: STK33 Antibody (6G1) [H00065975-M02] - STK33 monoclonal antibody (M02), clone 6G1 Analysis of STK33 expression in HeLa.](http://images.novusbio.com/fullsize/STK33-Antibody-6G1-Western-Blot-H00065975-M02-img0010.jpg "Western Blot: STK33 Antibody (6G1) [H00065975-M02] - STK33 monoclonal antibody (M02), clone 6G1 Analysis of STK33 expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: STK33 Antibody (6G1) [H00065975-M02] - Analysis of monoclonal antibody to STK33 on HeLa cell. Antibody concentration 25 ug/ml.](http://images.novusbio.com/fullsize/STK33-Antibody-6G1-Immunocytochemistry-Immunofluorescence-H00065975-M02-img0006.jpg)
![Western Blot: STK33 Antibody (6G1) [H00065975-M02] - Analysis of STK33 over-expressed 293 cell line, cotransfected with STK33 Validated Chimera RNAi ( Cat # H00065975-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with STK33 monoclonal antibody (M02) clone 6G1 (Cat # H00065975-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/STK33-Antibody-6G1-Western-Blot-H00065975-M02-img0008.jpg)
![Western Blot: STK33 Antibody (6G1) [H00065975-M02] - Analysis of STK33 expression in transfected 293T cell line by STK33 monoclonal antibody (M02), clone 6G1.Lane 1: STK33 transfected lysate(57.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/STK33-Antibody-6G1-Western-Blot-H00065975-M02-img0011.jpg)
![Immunoprecipitation: STK33 Antibody (6G1) [H00065975-M02] - Analysis of STK33 transfected lysate using anti-STK33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STK33 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/STK33-Antibody-6G1-Immunoprecipitation-H00065975-M02-img0007.jpg)
![Sandwich ELISA: STK33 Antibody (6G1) [H00065975-M02] - Detection limit for recombinant GST tagged STK33 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/STK33-Antibody-6G1-Sandwich-ELISA-H00065975-M02-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33064-img0007.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).")
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Immunofluorescent staining of MCF-7 cells with ErbB2/Her2 Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-33064-img0004.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Flow Cytometric Analysis of human trypsinized SK-BR3 cells. ErbB2/Her2 Antibody (HRB2/451) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Flow-Cytometry-NBP2-33064-img0002.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Flow Cytometric Analysis of human trypsinized MCF-7 cells. ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Flow-Cytometry-NBP2-33064-img0008.jpg)
![Protein Array: ErbB2/Her2 Antibody (HRB2/451) - Azide and BSA Free [NBP2-33064] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ErbB2/Her2 Antibody (HRB2/451) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Azide-and-BSA-Free-Protein-Array-NBP2-33064-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ZAP70 Antibody (2F3.2) [NBP2-44502] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeled with ZAP70 Antibody (2F3.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red).](http://images.novusbio.com/fullsize/ZAP70-Antibody-2F3-2-Immunocytochemistry-Immunofluorescence-NBP2-44502-img0006.jpg "Immunocytochemistry/Immunofluorescence: ZAP70 Antibody (2F3.2) [NBP2-44502] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeled with ZAP70 Antibody (2F3.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red).")
![Immunohistochemistry-Paraffin: ZAP70 Antibody (2F3.2) [NBP2-44502] - Human Tonsil stained with ZAP70 Monoclonal Antibody (2F3.2).](http://images.novusbio.com/fullsize/ZAP70-Antibody-2F3-2-Immunohistochemistry-Paraffin-NBP2-44502-img0001.jpg)
![Flow Cytometry: ZAP70 Antibody (2F3.2) [NBP2-44502] - Flow Cytometric Analysis of PFA-fixed Jurkat cells. ZAP70 Antibody (2F3.2) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/ZAP70-Antibody-2F3-2-Flow-Cytometry-NBP2-44502-img0005.jpg)
![Simple Western: ZAP70 Antibody (2F3.2) [NBP2-44502] - Simple Western lane view shows a specific band for ZAP70 in 0.2 mg/ml of Jurkat (left) and H. Tonsil (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ZAP70-Antibody-2F3-2-Simple-Western-NBP2-44502-img0002.jpg)
![Simple Western: ZAP70 Antibody (2F3.2) [NBP2-44502] - Electropherogram image of the corresponding Simple Western lane. ZAP70 antibody was used at 10 ug/ml dilution of Jurkat and H. Tonsil lysates(s) respectively.](http://images.novusbio.com/fullsize/ZAP70-Antibody-2F3-2-Simple-Western-NBP2-44502-img0003.jpg)
![Simple Western: ZAP70 Antibody (2F3.2) [NBP2-44502] - Lane view shows a specific band for ZAP70 in Jurkat cell lysate using 50 ug/mL antibody dilution. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 70 kDa. Image from an internal validation.](http://images.novusbio.com/fullsize/nbp2-44502_mouse-monoclonal-zap70-antibody-2f3-2-17720241003235.jpg)
![Western Blot: Fer Antibody [NBP1-20089] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Fer knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human Fer Polyclonal Antibody (Catalog # NBP1-20089) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Fer at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Fer-Antibody-Knockdown-Validated-NBP1-20089-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Fer Antibody [NBP1-20089] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Fer knockout (KO) HeLa cell line. PVDF membrane was probed with 0.5 ug/ml of Rabbit Anti-Human Fer Polyclonal Antibody (Catalog # NBP1-20089) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for Fer at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: FER Antibody [NBP1-20089] - Jurkat cell lysates.](http://images.novusbio.com/fullsize/FER-Antibody-Western-Blot-NBP1-20089-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FER Antibody [NBP1-20089] - FER antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/FER-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-20089-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Ret Antibody [NBP1-84568] - Staining of human cell line U-251 MG shows localization to cytosol & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ret-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84568-img0006.jpg "Immunocytochemistry/Immunofluorescence: Ret Antibody [NBP1-84568] - Staining of human cell line U-251 MG shows localization to cytosol & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Ret Antibody [NBP1-84568] - Staining of human gastrointestinal shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Ret-Antibody-Immunohistochemistry-Paraffin-NBP1-84568-img0011.jpg)
![Immunohistochemistry-Paraffin: Ret Antibody [NBP1-84568] - Staining of human adrenal gland shows cytoplasmic membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Ret-Antibody-Immunohistochemistry-Paraffin-NBP1-84568-img0008.jpg)
![Immunohistochemistry-Paraffin: Ret Antibody [NBP1-84568] - Staining of human cerebellum shows strong membranous positivity in Purkinje cells.](http://images.novusbio.com/fullsize/Ret-Antibody-Immunohistochemistry-Paraffin-NBP1-84568-img0009.jpg)
![Immunohistochemistry-Paraffin: Ret Antibody [NBP1-84568] - Staining of human endometrium shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Ret-Antibody-Immunohistochemistry-Paraffin-NBP1-84568-img0010.jpg)
![Western Blot: CrkRS Antibody [NB100-87011] - Detection of Human and Mouse CrkRS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody NB100-87011 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CrkRS-Antibody-Western-Blot-NB100-87011-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: CrkRS Antibody [NB100-87011] - Detection of Human and Mouse CrkRS by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody NB100-87011 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: CrkRS Antibody [NB100-87011] - Detection of human CrkRS by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CrkRS antibody NB100-87011 used for IP at 6 ug per reaction. For blotting immunoprecipitated CrkRS, NB100-87011 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CrkRS-Antibody-NB100-87011-img0005.jpg)
![Western Blot: JNK2 Antibody [NBP1-30905] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK2 antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/JNK2-Antibody-Western-Blot-NBP1-30905-img0007.jpg "Western Blot: JNK2 Antibody [NBP1-30905] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK2 antibody [N1C3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: JNK2 Antibody [NBP1-30905] - Analysis of methanol-fixed MCF-7, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/JNK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30905-img0002.jpg)
![Immunohistochemistry-Paraffin: JNK2 Antibody [NBP1-30905] - Paraffin-embedded N87 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/JNK2-Antibody-Immunohistochemistry-Paraffin-NBP1-30905-img0003.jpg)
![Western Blot: JNK2 Antibody [NBP1-30905] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/JNK2-Antibody-Western-Blot-NBP1-30905-img0004.jpg)
![Western Blot: JNK2 Antibody [NBP1-30905] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with JNK2 antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/JNK2-Antibody-Western-Blot-NBP1-30905-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - ICC/IF staining of MCF-7 cells with followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Immunocytochemistry-Immunofluorescence-NBP2-29437-img0009.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - ICC/IF staining of MCF-7 cells with followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Red Dot (Red).")
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - Flow Cytometric Analysis of human trypsinized MCF-7 cells. ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Flow-Cytometry-NBP2-29437-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - Immunofluorescent staining of PFA-fixed MCF-7 cells with ErbB2/Her2 Monospecific Mouse Monoclonal Antibody (HRB2/451) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are stained with Reddot (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Immunocytochemistry-Immunofluorescence-NBP2-29437-img0011.jpg)
![Flow Cytometry: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - Flow Cytometric Analysis of human trypsinized SK-BR3 cells. ErbB2/Her2 Antibody (HRB2/451) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Flow-Cytometry-NBP2-29437-img0008.jpg)
![Protein Array: ErbB2/Her2 Antibody (HRB2/451) [NBP2-29437] - Analysis of Protein Array containing more than 19,000 full-length human proteins using ErbB2/Her2 Antibody (HRB2/451) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD?s) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD?s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/ErbB2-Her2-Antibody-HRB2-451-Protein-Array-NBP2-29437-img0010.jpg)
![Western Blot: TIF1 alpha Antibody [NB100-2597] - Detection of Human TIF1 alpha/TRIM24 on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lysate (50 microgram) using NB100-2597. TIF1 alpha/TRIM24 was also immunoprecipitated by rabbit anti-TIF1 alpha/TRIM24 antibody NB100-2596.](http://images.novusbio.com/fullsize/TIF1-alpha-Antibody-Western-Blot-NB100-2597-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: TIF1 alpha Antibody [NB100-2597] - Detection of Human TIF1 alpha/TRIM24 on HeLa whole cell lysate (5, 15, and 50 microgram) and 293T cell lysate (50 microgram) using NB100-2597. TIF1 alpha/TRIM24 was also immunoprecipitated by rabbit anti-TIF1 alpha/TRIM24 antibody NB100-2596.")