| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-07986-100ul | |||
| BSA Free | NBP3-07986-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08380 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43110-0.5mg | |||
| BSA Free | NBP1-43110-0.1mg | |||
| BSA Free | NBP1-43110-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93296-0.02ml | |||
![Western Blot: VPS29 Antibody [NBP3-12366] - Rat lung extract. 1:500 antibody dilution in DiluObuffer. Apparent MW is 26 KDa.](http://images.novusbio.com/fullsize/VPS29-Antibody-Western-Blot-NBP3-12366-img0001.jpg "Western Blot: VPS29 Antibody [NBP3-12366] - Rat lung extract. 1:500 antibody dilution in DiluObuffer. Apparent MW is 26 KDa.")
![Immunohistochemistry-Paraffin: VPS29 Antibody [NBP3-12366] - Mouse Kidney. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/VPS29-Antibody-Immunohistochemistry-Paraffin-NBP3-12366-img0003.jpg)
![Immunohistochemistry-Paraffin: VPS29 Antibody [NBP3-12366] - Mouse Spleen. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/VPS29-Antibody-Immunohistochemistry-Paraffin-NBP3-12366-img0002.jpg)
![Western Blot: PPP2R4 Antibody [NBP1-51951] - Human Liver lysate (lane 1), Mouse Liver (lane 2) and Rat Liver (lane 3) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/PPP2R4-Antibody-Western-Blot-NBP1-51951-img0004.jpg "Western Blot: PPP2R4 Antibody [NBP1-51951] - Human Liver lysate (lane 1), Mouse Liver (lane 2) and Rat Liver (lane 3) (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: PPP2R4 Antibody [NBP1-51951] - (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PPP2R4-Antibody-Immunohistochemistry-Paraffin-NBP1-51951-img0005.jpg)
![Western Blot: SNAP23 Antibody [NBP1-32694] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP23 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Western-Blot-NBP1-32694-img0005.jpg "Western Blot: SNAP23 Antibody [NBP1-32694] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP23 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: SNAP23 Antibody [NBP1-32694] - (30 ug of whole cell lysate) A: H1299 12% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/SNAP23-Antibody-Western-Blot-NBP1-32694-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: SNAP23 Antibody [NBP1-32694] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32694-img0002.jpg)
![SNAP23 antibody detects SNAP23 protein at cell body and synaptic vesicles by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: SNAP23 protein stained by SNAP23 antibody (NBP1-32694) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-32694_rabbit-polyclonal-snap23-antibody-immunocytochemistry-immunofluorescence-141120251844222.jpg)
![Immunohistochemistry: PHLPP Antibody - BSA Free [NBP3-47157] - Immunohistochemistry of paraffin-embedded human liver slide using (PHLPP Antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-47157_rabbit-phlpp-pab-bsa-free-28420251235914.jpg "Immunohistochemistry: PHLPP Antibody - BSA Free [NBP3-47157] - Immunohistochemistry of paraffin-embedded human liver slide using (PHLPP Antibody) at dilution of 1:50")
![Immunocytochemistry/ Immunofluorescence: PHLPP Antibody - BSA Free [NBP3-47157] - Immunofluorescent analysis of HepG2 cells, using PHLPP antibody at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).](http://images.novusbio.com/fullsize/nbp3-47157_rabbit-phlpp-pab-bsa-free-28420251234385.jpg)
![Immunoprecipitation: PHLPP Antibody - BSA Free [NBP3-47157] - IP Result of anti-PHLPP (IP: , 4ug; Detection: 1:1000) with mouse brain tissue lysate 5000ug.](http://images.novusbio.com/fullsize/nbp3-47157_rabbit-phlpp-pab-bsa-free-28420251235634.jpg)
![Western Blot: PHLPP Antibody - BSA Free [NBP3-47157] - Mouse brain tissue were subjected to SDS PAGE followed by western blot with (PHLPP antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-47157_rabbit-phlpp-pab-bsa-free-284202512283321.jpg)
![Western Blot: Calcineurin A Antibody [NB110-40553] - Detection of human and mouse PPP3CA by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP3CA antibody NB110-40553 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Calcineurin-A-Antibody-Western-Blot-NB110-40553-img0009.jpg "Western Blot: Calcineurin A Antibody [NB110-40553] - Detection of human and mouse PPP3CA by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP3CA antibody NB110-40553 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: Calcineurin A Antibody [NB110-40553] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PPP3CA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Calcineurin-A-Antibody-Immunohistochemistry-NB110-40553-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Calcineurin A Antibody [NB110-40553] - Detection of human Calcineurin A (PPP3CA) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Calcineurin A antibody NB110-40553 used for IP at 3 ug per reaction. Calcineurin A was also immunoprecipitated by rabbit anti-Calcineurin A antibody NB110-40552. For blotting immunoprecipitated Calcineurin A, NB110-40553 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Calcineurin-A-Antibody-Immunoprecipitation-NB110-40553-img0010.jpg)
![Western Blot: CDC25C Antibody [NBP2-15827] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody](http://images.novusbio.com/fullsize/CDC25C-Antibody-Western-Blot-NBP2-15827-img0004.jpg "Western Blot: CDC25C Antibody [NBP2-15827] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDC25C antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody")
![Immunocytochemistry/Immunofluorescence: Cdc25C Antibody [NBP2-15827] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using CDC25C antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Cdc25C-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15827-img0003.jpg)
![Immunohistochemistry-Paraffin: Cdc25C Antibody [NBP2-15827] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Cdc25C-Antibody-Immunohistochemistry-Paraffin-NBP2-15827-img0002.jpg)
![Western Blot: Cdc25C Antibody [NBP2-15827] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/Cdc25C-Antibody-Western-Blot-NBP2-15827-img0001.jpg)
![Western Blot: ACP5 Antibody [H00000054-D01P] - Analysis of ACP5 expression in human liver.](http://images.novusbio.com/fullsize/ACP5-Antibody-Western-Blot-H00000054-D01P-img0003.jpg "Western Blot: ACP5 Antibody [H00000054-D01P] - Analysis of ACP5 expression in human liver.")
![Western Blot: ACP5 Antibody [H00000054-D01P] - Analysis of ACP5 expression in transfected 293T cell line by ACP5 polyclonal antibody.Lane 1: ACP5 transfected lysate(36.60 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/ACP5-Antibody-Western-Blot-H00000054-D01P-img0004.jpg)
![Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/Ser370) antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PTEN-[p-Thr366--p-Ser370]-Antibody-Western-Blot-NBP3-12919-img0004.jpg "Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/Ser370) antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/Ser370) antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PTEN-[p-Thr366--p-Ser370]-Antibody-Western-Blot-NBP3-12919-img0003.jpg)
![Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-12919_rabbit-pten-p-thr-366-p-ser-370-pab-9102024204126.jpg)
![Immunocytochemistry/ Immunofluorescence: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - PTEN antibody detects PTEN protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PTEN stained by PTEN antibody (NBP3-12919) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-12919_rabbit-pten-p-thr-366-p-ser-370-pab-91020242001095.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Western Blot Analysis of HeLa cell lysate using AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CPTC-CRYAB-1-Western-Blot-NBP3-07764-img0004.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Western Blot Analysis of HeLa cell lysate using AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).")
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Formalin-fixed, paraffin-embedded Rat Heart Muscle stained with AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CPTC-CRYAB-1-Immunohistochemistry-Paraffin-NBP3-07764-img0003.jpg)
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Formalin-fixed, paraffin-embedded human Breast stained with AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CYRAB-1).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CPTC-CRYAB-1-Immunohistochemistry-Paraffin-NBP3-07764-img0002.jpg)
![Protein Array: AlphaB Crystallin/CRYAB Antibody (CPTC-CRYAB-1) [NBP3-07764] - Analysis of Protein Array containing more than 19,000 full-length human proteins using AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CPTC-CRYAB-1).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-CPTC-CRYAB-1-Protein-Array-NBP3-07764-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Analysis using the Serum/Azide Free version of NBP1-43110. Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified -stimulated (purple histogram) C57Bl/6 splenocytes with 0.5 ug of Anti-Mouse CD279 (PD-1) FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-34497-img0004.jpg "Immunocytochemistry/Immunofluorescence: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Analysis using the Serum/Azide Free version of NBP1-43110. Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified -stimulated (purple histogram) C57Bl/6 splenocytes with 0.5 ug of Anti-Mouse CD279 (PD-1) FITC. Total viable cells were used for analysis.")
![Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Staining of 3-day unstimulated (left) and 3-day ConA activated (right) BALB/c splenocytes with 0.25 ug of Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse PD-1 Purified (filled histogram) followed by Anti-Armenian Hamster Ig](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Azide-Free-Flow-Cytometry-NBP2-34497-img0005.jpg)
![Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Staining of ConA stimulated splenocytes with Anti-Mouse CD279 (PD-1) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Azide-Free-Flow-Cytometry-NBP2-34497-img0001.jpg)
![Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Staining of unstimulated (orange histogram) or 3-day Anti-Mouse CD3 and Anti-Mouse CD28 Functional Grade Purified -stimulated (purple histogram) C57Bl/6 splenocytes with 0.5 ug of Anti-Mouse CD279 (PD-1) FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Azide-Free-Flow-Cytometry-NBP2-34497-img0003.jpg)
![Flow Cytometry: PD-1 Antibody (J43) - Azide Free [NBP2-34497] - Analysis of mouse Con-A activated spleen cell suspensions and mouse PD-1 transfected cells using PD1 PE conjugate. This can be used at less than or equal to 0.5 ug per test. Cell number should be determined empirically but can range from 10^5to 10^8 cells](http://images.novusbio.com/fullsize/PD-1-Antibody-J43-Azide-Free-Flow-Cytometry-NBP2-34497-img0002.jpg)
![Western Blot: PHLPP Antibody [NBP2-93296] - Western blot analysis of extracts of various cell lines, using PHLPP antibody (NBP2-93296) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/PHLPP Antibody-Western Blot-NBP2-93296-img0003.jpg "Western Blot: PHLPP Antibody [NBP2-93296] - Western blot analysis of extracts of various cell lines, using PHLPP antibody (NBP2-93296) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: PHLPP Antibody [NBP2-93296] - Immunohistochemistry of paraffin-embedded mouse kidney using PHLPP Rabbit pAb (NBP2-93296) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PHLPP-Antibody-Immunohistochemistry-Paraffin-NBP2-93296-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: PHLPP Antibody - Azide and BSA Free [NBP2-93296] - Immunofluorescence analysis of U-2 OS cells using PHLPP Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93296_rabbit-polyclonal-phlpp-antibody-4220251429019.jpg)