| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33192 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26422-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26442-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88134-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26206-50ul | |||
![Western Blot: SMCHD1 Antibody [NBP1-49969] - Detection of Human SmcHD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SmcHD1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SmcHD1 was also immunoprecipitated by rabbit anti-SmcHD1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated SmcHD1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Western-Blot-NBP1-49969-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SMCHD1 Antibody [NBP1-49969] - Detection of Human SmcHD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SmcHD1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SmcHD1 was also immunoprecipitated by rabbit anti-SmcHD1 antibody which recognizes an upstream epitope. For blotting immunoprecipitated SmcHD1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry: SMCHD1 Antibody [NBP1-49969] - Sample: FFPE section of human non-small cell lung cancer (left) and testicular seminoma (right). Antibody: Affinity purified rabbit anti- SmcHD1 used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Immunohistochemistry-NBP1-49969-img0002.jpg)
![Western Blot: YANK2 Antibody [NBP1-32343] - Sample(30 ug of whole cell lysate)A:HeLa S3 B:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/YANK2-Antibody-Western-Blot-NBP1-32343-img0004.jpg "Western Blot: YANK2 Antibody [NBP1-32343] - Sample(30 ug of whole cell lysate)A:HeLa S3 B:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: YANK2 Antibody [NBP1-32343] - Human gastric cancer, using STK32B antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/YANK2-Antibody-Immunohistochemistry-Paraffin-NBP1-32343-img0002.jpg)
![Western Blot: YANK2 Antibody [NBP1-32343] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/YANK2-Antibody-Western-Blot-NBP1-32343-img0003.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (C117/370) [NBP2-29423] - Formalin-fixed, paraffin-embedded human GIST stained with CD117/c-kit Mouse Monoclonal Antibody (C117/370).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-C117-370-Immunohistochemistry-Paraffin-NBP2-29423-img0005.jpg "Immunohistochemistry-Paraffin: CD117/c-kit Antibody (C117/370) [NBP2-29423] - Formalin-fixed, paraffin-embedded human GIST stained with CD117/c-kit Mouse Monoclonal Antibody (C117/370).")
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (C117/370) [NBP2-29423] - Formalin-fixed, paraffin-embedded human GIST stained with CD117/c-kit Antibody (C117/370).](http://images.novusbio.com/fullsize/CD117-c-kit-Antibody-C117-370-Immunohistochemistry-Paraffin-NBP2-29423-img0004.jpg)
![Immunohistochemistry-Paraffin: CD117/c-kit Antibody (C117/370) [NBP2-29423] - Formalin-paraffin human gastrointestinal stromal tumor(GIST) stained with CD117 Ab (C117/370).](http://images.novusbio.com/fullsize/nbp2-29423_mouse-monoclonal-cd117-c-kit-antibody-c117-370-imgenex-nbo-128a-20ug-17720241003246.jpg)
![Western Blot: DCLK1 Antibody (C1) [NBP3-41980] - Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Cerebrum lysate Primary Ab: 2ug/ml NBP3-41980; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41980_mouse-dclk1-mab-c1-612202421283814.jpg "Western Blot: DCLK1 Antibody (C1) [NBP3-41980] - Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Cerebrum lysate Primary Ab: 2ug/ml NBP3-41980; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: DCLK1 Antibody (C1) [NBP3-41980] - DAB staining on IHC-P; Sample: Human Cerebellum Tissue; Primary Ab: 30ug/ml NBP3-41980; Second Ab: 2ug/mLÂ HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41980_mouse-dclk1-mab-c1-612202421373015.jpg)
![Western Blot: DCLK1 Antibody (C1) [NBP3-41980] - Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Cerebrum lysate Primary Ab: 2ug/ml NBP3-41980; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41980_mouse-dclk1-mab-c1-61220242195713.jpg)
![Immunohistochemistry: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Image of GSK-3 beta [p Ser9] Antibody (3A6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-26220241525330.jpg "Immunohistochemistry: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Image of GSK-3 beta [p Ser9] Antibody (3A6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate (treated with Calyculin A or not). All lanes: GSK-3 beta [p Ser9] Antibody at 0.77ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46 KDa Observed band size: 46 KDa](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-28220241136300.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Staining of Hela cells(treated with 50mM Calyculin A for 30min) with GSK-3 beta [p Ser9] Antibody (3A6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-28220241312216.jpg)
![Immunoprecipitation: PAK2 Antibody (6D12) [NBP3-26442] - Immunoprecipitating PAK2 in Raji whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26442 in Raji whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26442 (2ug) + Raji whole cell lysate (500ug). Lane 3: Raji whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26442_rabbit-pak2-mab-6d12-26220241322151.jpg "Immunoprecipitation: PAK2 Antibody (6D12) [NBP3-26442] - Immunoprecipitating PAK2 in Raji whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26442 in Raji whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26442 (2ug) + Raji whole cell lysate (500ug). Lane 3: Raji whole cell lysate (10ug).")
![Western Blot: PAK2 Antibody (6D12) [NBP3-26442] - Positive Western Blot detected in: 293 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, Mouse brain tissue, Rat brain tissue. All lanes: PAK2 Antibody at 1:2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59 kDa Observed band size: 59 kDa](http://images.novusbio.com/fullsize/nbp3-26442_rabbit-pak2-mab-6d12-28220241140931.jpg)
![Flow Cytometry: PAK2 Antibody (6D12) [NBP3-26442] - Overlay histogram showing HepG2 cells stained with PAK2 Antibody (6D12) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26442_rabbit-pak2-mab-6d12-282202414183690.jpg)
![Immunocytochemistry/Immunofluorescence: PDGF R beta Antibody [NBP1-88134] - Staining of human cell line ASC TERT1 shows localization to the Golgi apparatus & vesicles.](http://images.novusbio.com/fullsize/PDGF-R-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88134-img0006.jpg "Immunocytochemistry/Immunofluorescence: PDGF R beta Antibody [NBP1-88134] - Staining of human cell line ASC TERT1 shows localization to the Golgi apparatus & vesicles.")
![Western Blot: CKII alpha Antibody [NBP2-15909] - Sample (30 ug of whole cell lysate) B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/CKII-alpha-Antibody-Western-Blot-NBP2-15909-img0002.jpg "Western Blot: CKII alpha Antibody [NBP2-15909] - Sample (30 ug of whole cell lysate) B: JC C: BCL-1 10% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: CKII alpha Antibody [NBP2-15909] - Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/CKII-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-15909-img0003.jpg)
![Western Blot: Casein Kinase 2 alpha Antibody [NBP2-15909] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody (NBP2-15909) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-15909_rabbit-polyclonal-casein-kinase-2-alpha-antibody-91020242014672.jpg)
![Immunocytochemistry/Immunofluorescence: BCR Antibody [NBP1-19097] - Human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody used at a dilution of 1:1,000 (1ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG - AlexaFluor 488 used at a dilution of 1:1,000.](http://images.novusbio.com/fullsize/BCR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-19097-img0009.jpg "Immunocytochemistry/Immunofluorescence: BCR Antibody [NBP1-19097] - Human K562 cells (contains the chromosomal translocation, t(9:22) that creates the BCR/ABL fusion gene). Antibody used at a dilution of 1:1,000 (1ug/ml). Detection: Green-fluorescent goat anti-rabbit IgG - AlexaFluor 488 used at a dilution of 1:1,000.")
![Flow Cytometry: BCR Antibody [NBP1-19097] - Flow Cytometric Detection of BCR. 1 x 106 K562 cells were fixed, permeabilized, and stained with 0.25 mcg anti-BCR in a 150 mcl reaction. Isotype anti-KLH control (black), no antibody (red) and anti-BCR (blue).](http://images.novusbio.com/fullsize/BCR-Antibody-Flow-Cytometry-NBP1-19097-img0006.jpg)
![Immunoprecipitation: BCR Antibody [NBP1-19097] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-BCR antibody used for IP at 10 mcg/mg lysate. BCR was also immunoprecipitated by rabbit anti-BCR antibody NBP1-19098, which recognizes a downstream epitope](http://images.novusbio.com/fullsize/BCR-Antibody-Immunoprecipitation-NBP1-19097-img0010.jpg)
![Immunohistochemistry: IKK beta Antibody (3E1) [NBP3-26206] - Image of IKK beta Antibody (3E1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26206_rabbit-ikk-beta-mab-3e1-262202415475613.jpg "Immunohistochemistry: IKK beta Antibody (3E1) [NBP3-26206] - Image of IKK beta Antibody (3E1) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: IKK beta Antibody (3E1) [NBP3-26206] - Positive Western Blot detected in: K562 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate. All lanes: IKK beta Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 87 kDa Observed band size: 87 kDa](http://images.novusbio.com/fullsize/nbp3-26206_rabbit-ikk-beta-mab-3e1-28220241140932.jpg)
![Immunocytochemistry/Immunofluorescence: IKK beta Antibody (3E1) [NBP3-26206] - Staining of Hela Cells with IKK beta Antibody (3E1) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26206_rabbit-ikk-beta-mab-3e1-2822024132367.jpg)