| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26442-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26422-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26535-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26542-50ul | |||
![Western Blot: ROCK2 Antibody [NB100-593] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROCK2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NB100-593-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: ROCK2 Antibody [NB100-593] - Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ROCK2 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: ROCK2 Antibody [NB100-593] - Detection of Human Rho Kinase Alpha on HeLa whole cell lysate using NB100-593. NB100-593 and NB100-592 were used at 5 ug/mg lysate to immunoprecipitate Rho Kinase Alpha.](http://images.novusbio.com/fullsize/ROCK2-Antibody-Western-Blot-NB100-593-img0002.jpg)
![Western Blot: DCLK1 Antibody (C1) [NBP3-41980] - Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Cerebrum lysate Primary Ab: 2ug/ml NBP3-41980; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41980_mouse-dclk1-mab-c1-612202421283814.jpg "Western Blot: DCLK1 Antibody (C1) [NBP3-41980] - Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Cerebrum lysate Primary Ab: 2ug/ml NBP3-41980; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody")
![Immunohistochemistry-Paraffin: DCLK1 Antibody (C1) [NBP3-41980] - DAB staining on IHC-P; Sample: Human Cerebellum Tissue; Primary Ab: 30ug/ml NBP3-41980; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41980_mouse-dclk1-mab-c1-612202421373015.jpg)
![Western Blot: DCLK1 Antibody (C1) [NBP3-41980] - Sample: Lane1: Porcine Cerebrum lysate; Lane2: Rat Cerebrum lysate Primary Ab: 2ug/ml NBP3-41980; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41980_mouse-dclk1-mab-c1-61220242195713.jpg)
![Immunoprecipitation: PAK2 Antibody (6D12) [NBP3-26442] - Immunoprecipitating PAK2 in Raji whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26442 in Raji whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26442 (2ug) + Raji whole cell lysate (500ug). Lane 3: Raji whole cell lysate (10ug).](http://images.novusbio.com/fullsize/nbp3-26442_rabbit-pak2-mab-6d12-26220241322151.jpg "Immunoprecipitation: PAK2 Antibody (6D12) [NBP3-26442] - Immunoprecipitating PAK2 in Raji whole cell lysate. Lane 1: Rabbit control IgG instead of NBP3-26442 in Raji whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26442 (2ug) + Raji whole cell lysate (500ug). Lane 3: Raji whole cell lysate (10ug).")
![Western Blot: PAK2 Antibody (6D12) [NBP3-26442] - Positive Western Blot detected in: 293 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate, Mouse brain tissue, Rat brain tissue. All lanes: PAK2 Antibody at 1:2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 59 kDa Observed band size: 59 kDa](http://images.novusbio.com/fullsize/nbp3-26442_rabbit-pak2-mab-6d12-28220241140931.jpg)
![Flow Cytometry: PAK2 Antibody (6D12) [NBP3-26442] - Overlay histogram showing HepG2 cells stained with PAK2 Antibody (6D12) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 1 h at 4C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26442_rabbit-pak2-mab-6d12-282202414183690.jpg)
![Western Blot: YANK2 Antibody [NBP1-32343] - Sample(30 ug of whole cell lysate)A:HeLa S3 B:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/YANK2-Antibody-Western-Blot-NBP1-32343-img0004.jpg "Western Blot: YANK2 Antibody [NBP1-32343] - Sample(30 ug of whole cell lysate)A:HeLa S3 B:Hep G2 7. 5% SDS PAGE, antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: YANK2 Antibody [NBP1-32343] - Human gastric cancer, using STK32B antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/YANK2-Antibody-Immunohistochemistry-Paraffin-NBP1-32343-img0002.jpg)
![Western Blot: YANK2 Antibody [NBP1-32343] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/YANK2-Antibody-Western-Blot-NBP1-32343-img0003.jpg)
![Immunohistochemistry: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Image of GSK-3 beta [p Ser9] Antibody (3A6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-26220241525330.jpg "Immunohistochemistry: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Image of GSK-3 beta [p Ser9] Antibody (3A6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Western Blot: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate (treated with Calyculin A or not). All lanes: GSK-3 beta [p Ser9] Antibody at 0.77ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 46 KDa Observed band size: 46 KDa](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-28220241136300.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Staining of Hela cells(treated with 50mM Calyculin A for 30min) with GSK-3 beta [p Ser9] Antibody (3A6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26422_rabbit-gsk-3-beta-p-ser-9-mab-3a6-28220241312216.jpg)
![Western Blot: EphA10 Antibody [NBP3-41254] - Sample: Rat Testis lysate; Primary Ab: 1ug/ml NBP3-41254; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41254_rabbit-epha10-pab-61220242195729.jpg "Western Blot: EphA10 Antibody [NBP3-41254] - Sample: Rat Testis lysate; Primary Ab: 1ug/ml NBP3-41254; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody")
![<b>Genetic Strategies Validation. </b>Western Blot: EphA10 Antibody [NBP3-41254] - Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: EPHA10 knockout K562 cell lysate; Predicted MW: 32,97,110kd ; Observed MW: 90kd; Primary Ab: 1ug/ml NBP3-41254; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;](http://images.novusbio.com/fullsize/nbp3-41254_rabbit-epha10-pab-612202421395327.jpg)
![Western Blot: EphA10 Antibody [NBP3-41254] - Sample: Human K562 cell lysate; Primary Ab: 1ug/ml NBP3-41254; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-41254_rabbit-epha10-pab-6122024212766.jpg)
![Western Blot: SMCHD1 Antibody [NBP1-49968] - Detection of Human SmcHD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SmcHD1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SmcHD1 was also immunoprecipitated by rabbit anti-SmcHD1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated SmcHD1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Western-Blot-NBP1-49968-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SMCHD1 Antibody [NBP1-49968] - Detection of Human SmcHD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-SmcHD1 antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. SmcHD1 was also immunoprecipitated by rabbit anti-SmcHD1 antibody which recognizes a downstream epitope. For blotting immunoprecipitated SmcHD1 was used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Immunohistochemistry-Paraffin: SMCHD1 Antibody [NBP1-49968] - Sample: FFPE section of human testicular seminoma (left) and non-small cell lung cancer (right). Antibody: Affinity purified rabbit anti- SmcHD1 used at a dilution of 1:200 (1ug/ml). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate](http://images.novusbio.com/fullsize/SMCHD1-Antibody-Immunohistochemistry-NBP1-49968-img0004.jpg)
![Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-2622024151064.jpg "Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.")
![Immunohistochemistry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Image of HIST1H3A [ac Lys18] Antibody (30A7) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.07% DAB.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-272202485630.jpg)
![Immunocytochemistry/Immunofluorescence: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Staining of A549 with HIST1H3A [ac Lys18] Antibody (30A7) at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 490-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-282202412493638.jpg)
![Flow Cytometry: HIST1H3A [ac Lys18] Antibody (30A7) [NBP3-26535] - Overlay Peak curve showing A549 cells stained with HIST1H3A [ac Lys18] Antibody (30A7) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26535_rabbit-hist1h3a-ac-lys-18-mab-30a7-282202414312785.jpg)
![Western Blot: STK40 Antibody [NBP2-20519] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with STK40 antibody [N1N3] (NBP2-20519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20519_rabbit-polyclonal-stk40-antibody-910202420122082.jpg "Western Blot: STK40 Antibody [NBP2-20519] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with STK40 antibody [N1N3] (NBP2-20519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: STK40 Antibody [NBP2-20519] - Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/STK40-Antibody-Western-Blot-NBP2-20519-img0001.jpg)
![STK40 antibody [N1N3] detects STK40 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: STK40 protein stained by STK40 antibody [N1N3] (NBP2-20519) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-20519_rabbit-polyclonal-stk40-antibody-immunocytochemistry-immunofluorescence-2611202518281010.jpg)
![Immunohistochemistry: GSK-3 beta Antibody (9G11) [NBP3-26542] - Image of GSK-3 beta Antibody (9G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26542_rabbit-gsk-3-beta-mab-9g11-262202414471519.jpg "Immunohistochemistry: GSK-3 beta Antibody (9G11) [NBP3-26542] - Image of GSK-3 beta Antibody (9G11) diluted at 1:100 and staining in paraffin-embedded human breast cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: GSK-3 beta Antibody (9G11) [NBP3-26542] - Positive Western Blot detected in: Hela whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, 293 whole cell lysate. All lanes: GSK3 beta Antibody at 1: 1000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 47, 49 kDa Observed band size: 47 kDa](http://images.novusbio.com/fullsize/nbp3-26542_rabbit-gsk-3-beta-mab-9g11-28220241140910.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 beta Antibody (9G11) [NBP3-26542] - Staining of Hela Cells with GSK-3 beta Antibody (9G11) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26542_rabbit-gsk-3-beta-mab-9g11-282202412475130.jpg)
![Immunohistochemistry-Paraffin: TTBK1 Antibody [NBP3-42338] - DAB staining on IHC-P; Samples: Human Glioma Tissue; Primary Ab: 10ug/ml NBP3-42338; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody](http://images.novusbio.com/fullsize/nbp3-42338_rabbit-ttbk1-pab-612202421243248.jpg "Immunohistochemistry-Paraffin: TTBK1 Antibody [NBP3-42338] - DAB staining on IHC-P; Samples: Human Glioma Tissue; Primary Ab: 10ug/ml NBP3-42338; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody")
![Western Blot: TTBK1 Antibody [NBP3-42338] - Sample: Recombinant protein.](http://images.novusbio.com/fullsize/nbp3-42338_rabbit-ttbk1-pab-61220242137301.jpg)
![Immunohistochemistry-Paraffin: TTBK1 Antibody [NBP3-42338] - DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 10ug/ml NBP3-42338](http://images.novusbio.com/fullsize/nbp3-42338_rabbit-ttbk1-pab-612202421331642.jpg)
![Western Blot: RIOK2 Antibody [NBP1-30099] - Whole cell lysate from HeLa cells. RIOK2 was also immunoprecipitated by rabbit anti-RIOK2 antibody NBP1-30098. For blotting IPed RIOK2, the ReliaBLOT Reagents and Procedures WB120 were used.](http://images.novusbio.com/fullsize/RIOK2-Antibody-Western-Blot-NBP1-30099-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: RIOK2 Antibody [NBP1-30099] - Whole cell lysate from HeLa cells. RIOK2 was also immunoprecipitated by rabbit anti-RIOK2 antibody NBP1-30098. For blotting IPed RIOK2, the ReliaBLOT Reagents and Procedures WB120 were used.")