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![Western Blot: Androgen R/NR3C4 [p Ser94] Antibody [NBP2-29527] - HeLa lysate showing specific immunolabeling of the ~110 kD AR protein phosphorylated at Ser94. The phosphospecificity is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen.](http://images.novusbio.com/fullsize/Androgen-Receptor-[p-Ser94]-Antibody-Western-Blot-NBP2-29527-img0001.jpg "Western Blot: Androgen R/NR3C4 [p Ser94] Antibody [NBP2-29527] - HeLa lysate showing specific immunolabeling of the ~110 kD AR protein phosphorylated at Ser94. The phosphospecificity is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen.")
![Western Blot: JAM-B/VE-JAM Antibody (MM0425-4L28) [NBP2-11745] - Lysates of HEK293 overexpressing human JJAM-B/VE-JAM.](http://images.novusbio.com/fullsize/JAM-B-VE-JAM-Antibody-MM0425-4L28-Western-Blot-NBP2-11745-img0001.jpg "Western Blot: JAM-B/VE-JAM Antibody (MM0425-4L28) [NBP2-11745] - Lysates of HEK293 overexpressing human JJAM-B/VE-JAM.")
![Western Blot: SH3BP2 Antibody [NBP3-30802] - Western blot analysis using anti-3BP2 at 10 ug/ml on recombinant full length 3BP2 protein (1), 3BP2 protein inus the PH domain (2), 3BP2 protein minus the PR domain (3) and 3BP2 protein minus the SH2 domain (4).](http://images.novusbio.com/fullsize/nbp3-30802_sheep-sh3bp2-pab-811202420594225.jpg "Western Blot: SH3BP2 Antibody [NBP3-30802] - Western blot analysis using anti-3BP2 at 10 ug/ml on recombinant full length 3BP2 protein (1), 3BP2 protein inus the PH domain (2), 3BP2 protein minus the PR domain (3) and 3BP2 protein minus the SH2 domain (4).")
![Immunohistochemistry: Calcitonin Antibody (PD00-46) [NBP3-32101] - Immunohistochemical analysis of paraffin-embedded human medullary thyroid carcinoma tissue with Rabbit anti-Calcitonin antibody (NBP3-32101) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32101) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32101_rabbit-calcitonin-mab-pd00-46-298202414262610.jpg "Immunohistochemistry: Calcitonin Antibody (PD00-46) [NBP3-32101] - Immunohistochemical analysis of paraffin-embedded human medullary thyroid carcinoma tissue with Rabbit anti-Calcitonin antibody (NBP3-32101) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32101) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: STAT1 [p Ser727] Antibody (PSM1) [NB500-515] - Analysis (reducing conditions) of phosphorylated STAT1 (Ser727) in IFN-a treated HeLa human cervix carcinoma cell line using anti-Phospho STAT1 (PSM1). Lane 1: immunoprecipitated material by anti-STAT1 (SM2) Lane 2: original whole cell lysate.](http://images.novusbio.com/fullsize/p_6190_1.jpg "Western Blot: STAT1 [p Ser727] Antibody (PSM1) [NB500-515] - Analysis (reducing conditions) of phosphorylated STAT1 (Ser727) in IFN-a treated HeLa human cervix carcinoma cell line using anti-Phospho STAT1 (PSM1). Lane 1: immunoprecipitated material by anti-STAT1 (SM2) Lane 2: original whole cell lysate.")
![Western Blot: c-Myb Antibody (JE40-07) [NBP3-32200] - Western blot analysis of c-Myb on different lysates with Rabbit anti-c-Myb antibody (NBP3-32200) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: 3 minutes 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32200) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32200_rabbit-c-myb-mab-je40-07-278202415242635.jpg "Western Blot: c-Myb Antibody (JE40-07) [NBP3-32200] - Western blot analysis of c-Myb on different lysates with Rabbit anti-c-Myb antibody (NBP3-32200) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: HL-60 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: 3 minutes 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32200) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: p38 alpha Antibody (0004) [NBP3-23507] - Immunohistochemical analysis of paraffin-embedded human carcinoma of sigmoid tissue,untreated (left) or lambda phosphatase-treated (right), using NBP3-23507 at 1:500 dilution.](http://images.novusbio.com/fullsize/nbp3-23507_rabbit-p38-alpha-mab-0004-235202511444436.png "Immunohistochemistry-Paraffin: p38 alpha Antibody (0004) [NBP3-23507] - Immunohistochemical analysis of paraffin-embedded human carcinoma of sigmoid tissue,untreated (left) or lambda phosphatase-treated (right), using NBP3-23507 at 1:500 dilution.")
![Western Blot: PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42) [NBP3-32784] - Western blot analysis of PAK4+5+6 on different lysates with Rabbit anti-PAK4+5+6 antibody (NBP3-32784) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: HUVEC cell lysate (10 ug/Lane) Lane 5: HUVEC treated with 50ng/mL VEGF for 2 minutes cell lysate (30 ug/Lane) Predicted band size: 64/81/75 kDa Observed band size: 64 kDa Exposure time: 30 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32784) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32784_rabbit-pak4-5-6-p-ser602-p-ser474-p-ser560-mab-je57-42-288202412442919.jpg "Western Blot: PAK4+5+6 [p Ser602, p Ser474, p Ser560] Antibody (JE57-42) [NBP3-32784] - Western blot analysis of PAK4+5+6 on different lysates with Rabbit anti-PAK4+5+6 antibody (NBP3-32784) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: HeLa cell lysate (20 ug/Lane) Lane 3: MCF7 cell lysate (20 ug/Lane) Lane 4: HUVEC cell lysate (10 ug/Lane) Lane 5: HUVEC treated with 50ng/mL VEGF for 2 minutes cell lysate (30 ug/Lane) Predicted band size: 64/81/75 kDa Observed band size: 64 kDa Exposure time: 30 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32784) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD5 Antibody (PD00-01) [NBP3-32158] - Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD5 antibody (NBP3-32158) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32158) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32158_rabbit-cd5-mab-pd00-01-298202414115417.jpg "Immunohistochemistry: CD5 Antibody (PD00-01) [NBP3-32158] - Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-CD5 antibody (NBP3-32158) at 1/1,500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32158) at 1/1,500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: ER alpha/NR3A1 Antibody (PD00-04) [NBP3-32321] - Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-ER alpha/NR3A1 antibody (NBP3-32321) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32321) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32321_rabbit-er-alpha-nr3a1-mab-pd00-04-298202413585568.jpg "Immunohistochemistry: ER alpha/NR3A1 Antibody (PD00-04) [NBP3-32321] - Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-ER alpha/NR3A1 antibody (NBP3-32321) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32321) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: ODF2 Antibody (JE35-01) [NBP3-32676] - Western blot analysis of ODF2 on different lysates with Rabbit anti-ODF2 antibody (NBP3-32676) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: K-562 cell lysate Lane 3: MCF7 cell lysate Lane 4: HeLa cell lysate Lane 5: U-2 OS cell lysate Lane 6: Jurkat cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 95 kDa Observed band size: 95 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32676) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32676_rabbit-odf2-mab-je35-01-278202416285286.jpg "Western Blot: ODF2 Antibody (JE35-01) [NBP3-32676] - Western blot analysis of ODF2 on different lysates with Rabbit anti-ODF2 antibody (NBP3-32676) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: K-562 cell lysate Lane 3: MCF7 cell lysate Lane 4: HeLa cell lysate Lane 5: U-2 OS cell lysate Lane 6: Jurkat cell lysate Lane 7: HepG2 cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 95 kDa Observed band size: 95 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32676) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Western Blot: TOR/mTOR [p Ser2481] Antibody (JE59-62) [NBP3-32779] - Western blot analysis of TOR/mTOR on different lysates with Rabbit anti-TOR/mTOR antibody (NBP3-32779) at 1/5,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa starved overnight then treated with 200nM PMA for 4 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 289 kDa Observed band size: 289 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32779) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32779_rabbit-tor-mtor-p-ser2481-mab-je59-62-288202412461671.jpg "Western Blot: TOR/mTOR [p Ser2481] Antibody (JE59-62) [NBP3-32779] - Western blot analysis of TOR/mTOR on different lysates with Rabbit anti-TOR/mTOR antibody (NBP3-32779) at 1/5,000 dilution. Lane 1: HeLa whole cell lysate Lane 2: HeLa starved overnight then treated with 200nM PMA for 4 hours whole cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 289 kDa Observed band size: 289 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32779) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")