| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18566-25ul | |||
| Azide and BSA Free | NBP3-44375 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18578-25ul | |||
| Azide and BSA Free | NBP3-44376 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89818-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21205-25ul | |||
| Azide and BSA Free | NBP3-44327 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18580-25ul | |||
| Azide and BSA Free | NBP3-44377 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81579-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80879-25ul | |||
![Western Blot: CD44 Antibody (CL13324) [NBP3-18566] - Analysis in human cell line U-251 MG and human cell line MCF-7.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Western-Blot-NBP3-18566-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: CD44 Antibody (CL13324) [NBP3-18566] - Analysis in human cell line U-251 MG and human cell line MCF-7.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD44 Antibody (CL13324) [NBP3-18566] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Immunohistochemistry-Paraffin-NBP3-18566-img0008.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13324) [NBP3-18566] - Analysis in human cervix, uterine and skeletal muscle tissues using NBP3-18566 antibody. Corresponding CD44 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Immunohistochemistry-Paraffin-NBP3-18566-img0001.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13324) [NBP3-18566] - Staining of human ovarian cancer (endometrioid carcinoma) shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Immunohistochemistry-Paraffin-NBP3-18566-img0002.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13324) [NBP3-18566] - Staining of human cervical cancer shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Immunohistochemistry-Paraffin-NBP3-18566-img0003.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13324) [NBP3-18566] - Staining of human ovarian cancer (low grade carcinoma) shows moderate membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Immunohistochemistry-Paraffin-NBP3-18566-img0004.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13324) [NBP3-18566] - Staining of human uterine cervix shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Immunohistochemistry-Paraffin-NBP3-18566-img0005.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13324) [NBP3-18566] - Staining of human lymph node shows strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Immunohistochemistry-Paraffin-NBP3-18566-img0006.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13324) [NBP3-18566] - Staining of human colon shows strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13324-Immunohistochemistry-Paraffin-NBP3-18566-img0007.jpg)
![Western Blot: CD44 Antibody (CL13318) [NBP3-18578] - Analysis in human cell line U-251 MG and human cell line MCF-7.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Western-Blot-NBP3-18578-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: CD44 Antibody (CL13318) [NBP3-18578] - Analysis in human cell line U-251 MG and human cell line MCF-7.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0008.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Analysis in human skin and skeletal muscle tissues using NBP3-18578 antibody. Corresponding CD44 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0001.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human cervical cancer shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0002.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human breast cancer (metastasis to ovary) shows moderate membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0003.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human malignant melanoma shows moderate membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0004.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human skin shows strong membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0005.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human tonsil shows strong membranous positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0006.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18578] - Staining of human colon shows strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18578-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody [NBP1-89818] - Staining of human cell line A-431 shows localization to nuclear bodies. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89818-img0008.jpg "Immunocytochemistry/Immunofluorescence: Alpha Actinin 4 Antibody [NBP1-89818] - Staining of human cell line A-431 shows localization to nuclear bodies. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Alpha Actinin 4 Antibody [NBP1-89818] - Staining of human liver shows strong cytoplasmic and membranous positivity in bile duct cells.](http://images.novusbio.com/fullsize/Alpha-Actinin-4-Antibody-Immunohistochemistry-Paraffin-NBP1-89818-img0001.jpg)
![Western Blot: CD44 Antibody (CL13318) [NBP3-18580] - Analysis in human cell line U-251 MG and human cell line MCF-7.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Western-Blot-NBP3-18580-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: CD44 Antibody (CL13318) [NBP3-18580] - Analysis in human cell line U-251 MG and human cell line MCF-7.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18580] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18580-img0008.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18580] - Analysis in human cervix, uterine and skeletal muscle tissues using NBP3-18580 antibody. Corresponding CD44 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18580-img0001.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18580] - Staining of human endometrial cancer shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18580-img0002.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18580] - Staining of human breast cancer (metastasis to ovary) shows moderate membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18580-img0003.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18580] - Staining of human malignant melanoma shows moderate membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18580-img0004.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18580] - Staining of human lymph node shows strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18580-img0005.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18580] - Staining of human uterine cervix shows moderate membranous positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18580-img0006.jpg)
![Immunohistochemistry-Paraffin: CD44 Antibody (CL13318) [NBP3-18580] - Staining of human colon shows strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD44-Antibody-CL13318-Immunohistochemistry-Paraffin-NBP3-18580-img0007.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Analysis in human urinary bladder and skeletal muscle tissues using NBP1-81579 antibody. Corresponding STAT1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/STAT1-Antibody-NBP1-81579-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Analysis in human urinary bladder and skeletal muscle tissues using NBP1-81579 antibody. Corresponding STAT1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: STAT1 Antibody [NBP1-81579] - Analysis in human cell lines SK-MEL-30 and HEK293 using Anti-STAT1 antibody. Corresponding STAT1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/STAT1-Antibody-Western-Blot-NBP1-81579-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: STAT1 Antibody [NBP1-81579] - Staining of human cell line A-431 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81579-img0013.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Staining of human skeletal muscle shows very weak positivity in myocytes.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-81579-img0022.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Staining of human lymph node shows cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-81579-img0017.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urotheilal cells.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-81579-img0020.jpg)
![Immunohistochemistry-Paraffin: STAT1 Antibody [NBP1-81579] - Staining of human prostate shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/STAT1-Antibody-Immunohistochemistry-Paraffin-NBP1-81579-img0021.jpg)
![Immunohistochemistry-Paraffin: PKA 2 beta Antibody [NBP1-80879] - Analysis in human adipose tissue and skeletal muscle tissues using NBP1-80879 antibody. Corresponding PKA 2 beta RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PKA-2-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-80879-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PKA 2 beta Antibody [NBP1-80879] - Analysis in human adipose tissue and skeletal muscle tissues using NBP1-80879 antibody. Corresponding PKA 2 beta RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: PKA 2 beta Antibody [NBP1-80879] - Analysis in human cell line Daudi.](http://images.novusbio.com/fullsize/PKA-2-beta-Antibody-Western-Blot-NBP1-80879-img0006.jpg)
![Immunohistochemistry-Paraffin: PKA 2 beta Antibody [NBP1-80879] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/PKA-2-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-80879-img0013.jpg)
![Immunohistochemistry-Paraffin: PKA 2 beta Antibody [NBP1-80879] - Staining of human soft tissue shows distinct positivity in adipocytes.](http://images.novusbio.com/fullsize/PKA-2-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-80879-img0007.jpg)
![Immunohistochemistry-Paraffin: PKA 2 beta Antibody [NBP1-80879] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PKA-2-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-80879-img0011.jpg)
![Immunohistochemistry-Paraffin: PKA 2 beta Antibody [NBP1-80879] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/PKA-2-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-80879-img0012.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp3-52088_mouse-mbp-mab-cl2829-western-blot-273202615204516.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp3-52088_mouse-mbp-mab-cl2829-western-blot-27320261527554.jpg)
![Western Blot: MEK1 [p Thr386] Antibody [NB500-144] - Analysis of recombinant WT and mutant MEK 1 immunolabeled with the Anti Thr386 MEK1 antibody. Lanes 1 and 2 are WT MEK 1 and Lanes 3 and 4 are mutant MEK 1 (T386A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.](http://images.novusbio.com/fullsize/MEK1-[p-Thr386]-Antibody-Western-Blot-NB500-144-img0001.jpg "Western Blot: MEK1 [p Thr386] Antibody [NB500-144] - Analysis of recombinant WT and mutant MEK 1 immunolabeled with the Anti Thr386 MEK1 antibody. Lanes 1 and 2 are WT MEK 1 and Lanes 3 and 4 are mutant MEK 1 (T386A). MAP kinase was coexpressed in the samples run in Lanes 2 and 4.")
![Immunocytochemistry/Immunofluorescence: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Staining phospho-PKA R2 (S99) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PKA-R2-p-Ser99-Antibody-SC54-04-Immunocytochemistry-Immunofluorescence-NBP2-67571-img0002.jpg "Immunocytochemistry/Immunofluorescence: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Staining phospho-PKA R2 (S99) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Analysis of paraffin-embedded mouse testis tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PKA-R2-p-Ser99-Antibody-SC54-04-Immunohistochemistry-Paraffin-NBP2-67571-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Staining phospho-PKA R2 (S99) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/PKA-R2-p-Ser99-Antibody-SC54-04-Immunocytochemistry-Immunofluorescence-NBP2-67571-img0001.jpg)
![Immunohistochemistry-Paraffin: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Analysis of paraffin-embedded human spleen tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PKA-R2-p-Ser99-Antibody-SC54-04-Immunohistochemistry-Paraffin-NBP2-67571-img0003.jpg)
![Immunohistochemistry-Paraffin: PKA R2 [p Ser99] Antibody (SC54-04) [NBP2-67571] - Analysis of paraffin-embedded mouse heart tissue using anti- phospho-PKA R2 (S99) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/PKA-R2-p-Ser99-Antibody-SC54-04-Immunohistochemistry-Paraffin-NBP2-67571-img0004.jpg)
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using Histone H4 [Trimethyl Lys20] Antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NBP2-59259-img0006.jpg "Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using Histone H4 [Trimethyl Lys20] Antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.")
![Immunocytochemistry/Immunofluorescence: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - (Top): Human osteosarcoma (U2OS) cells were stained with and DAPI. Cells were fixed with ice cold methanol for 10' and blocked with PBS/TX-100 containing 5% normal goat serum. Figure 6A: cells were immunofluorescently labeled with (left) diluted 1:300 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa568 or with DAPI (right), which specifically labels DNA. (Bottom): Staining of the cells with the after incubation of the antibody with blocking peptide, concentration: 5 ng/ul.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-59259-img0005.jpg)
![Chromatin Immunoprecipitation: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - ChIP assays were performed using undifferentiated human teratocarcinoma cells (NCCIT), the antibody against H4K20me3 and optimized PCR primer sets for qPCR. Sheared chromatin from 10,000 cells was used per ChIP experiment. The antibody was tested at two different dilutions of 1:500 and 1:100. The pre-immune serum (PI) was used as a negative control. Quantitative PCR was performed using primer sets for the satellite repeat Sat2 as a positive control and for the promoter of the house keeping gene c-fos, as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis). These results are in accordance with the observation that H4K20me3 is preferably present at heterochromatin.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Chromatin-Immunoprecipitation-NBP2-59259-img0004.jpg)
![Western Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - Histone (acid) extracts of NB4 cells were analysed by Western blot using the Diagenode antibody against H4K20me3, diluted 1:750 in TBS-Tween containing 5% skimmed milk. The location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Western-Blot-NBP2-59259-img0002.jpg)
![Dot Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - Dot Blot was used to check the specificity of the antibody against H4K20me3 with peptides containing other modifications of histone H3 and H4. Other histone modifications include monoand dimethylation of the same lysine and mono-, di- and trimethylation of lysines 9, 27 and 36 of H3. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. The antibody was used at a dilution of 1:1,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Dot-Blot-NBP2-59259-img0001.jpg)
![ELISA: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - To determine the titer, an ELISA was performed using a serial dilution of the Diagenode antibody directed against H4K20me3. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:700.](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-ELISA-NBP2-59259-img0003.jpg)
![Dot Blot: Histone H4 [Trimethyl Lys20] Antibody [NBP2-59259] - A Dot Blot analysis was performed to test the cross reactivity of Histone H4 [Trimethyl Lys20] Antibody with peptides containing other histone modifications and the unmodified H4K20. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest](http://images.novusbio.com/fullsize/Histone-H4-[Trimethyl-Lys20]-Antibody-Dot-Blot-NBP2-59259-img0007.jpg)