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![Western Blot: p53 [p Thr81] Antibody [NBP3-16955] - Western blot analysis of extracts of C6 cells, using p53antibody (NBP3-16955) at 1:500 dilution.C6 cells were treated by nocodazole (50 ng/ml) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/p53-[p-Thr81]-Antibody-Western-Blot-NBP3-16955-img0001.jpg "Western Blot: p53 [p Thr81] Antibody [NBP3-16955] - Western blot analysis of extracts of C6 cells, using p53antibody (NBP3-16955) at 1:500 dilution.C6 cells were treated by nocodazole (50 ng/ml) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: PP2A alpha [p Tyr307] Antibody (9B7Z9) [NBP3-16919] - Western blot analysis of extracts of NIH/3T3 cells, using PP2A alpha Rabbit mAb (NBP3-16919) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/PP2A-alpha-p-Tyr307-Antibody-9B7Z9-Western-Blot-NBP3-16919-img0001.jpg "Western Blot: PP2A alpha [p Tyr307] Antibody (9B7Z9) [NBP3-16919] - Western blot analysis of extracts of NIH/3T3 cells, using PP2A alpha Rabbit mAb (NBP3-16919) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 3s.")
![Western Blot: NMDAR2C [p Ser1244] Antibody [NBP2-29536] - Western blot of rat cerebellum lysate showing specific immunolabeling of the ~140k NR2C subunit of the NMDA receptor phosphorylated at Ser1244. The phosphospecificity is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen but not by the dephosphopeptide (not shown).](http://images.novusbio.com/fullsize/NMDAR2C-[p-Ser1244]-Antibody-Western-Blot-NBP2-29536-img0001.jpg "Western Blot: NMDAR2C [p Ser1244] Antibody [NBP2-29536] - Western blot of rat cerebellum lysate showing specific immunolabeling of the ~140k NR2C subunit of the NMDA receptor phosphorylated at Ser1244. The phosphospecificity is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen but not by the dephosphopeptide (not shown).")
![Western Blot: General Receptor for phosphoinositides 1 Antibody [NB300-310] - Rat cerebellar lysate showing the specific immunolabeling of the ~95k GRASP protein, using NB300-310.](http://images.novusbio.com/fullsize/General-Receptor-for-phosphoinositides-1-Antibody-Western-Blot-NB300-310-img0001.jpg "Western Blot: General Receptor for phosphoinositides 1 Antibody [NB300-310] - Rat cerebellar lysate showing the specific immunolabeling of the ~95k GRASP protein, using NB300-310.")
![Western Blot: mGluR7 [p Ser862] Antibody [NBP1-47557] - Mouse brain lysate demonstrating specific immunolableing of the 102kDa mGluR7 [p Ser862] Immunolabeling is blocked by the phospho-peptide used as immunogen (peptide).](http://images.novusbio.com/fullsize/mGluR7-[p-Ser862]-Antibody-Western-Blot-NBP1-47557-img0005.jpg "Western Blot: mGluR7 [p Ser862] Antibody [NBP1-47557] - Mouse brain lysate demonstrating specific immunolableing of the 102kDa mGluR7 [p Ser862] Immunolabeling is blocked by the phospho-peptide used as immunogen (peptide).")
![Western Blot: Tryptophan Hydroxylase 1 [p Ser260] Antibody [NB300-307] - Rat brainstem lysate showing specific immunolabeling of the ~55k TPH phosphorylated at Ser260. The labeling by Anti-Ser260TPH is specifically blocked by the Ser260 phosphopeptide (Phos-pep) used as antigen. The corresponding nonphosphopeptide (Nonphos-pep) did not block the immunolabeling.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-[p-Ser260]-Antibody-Western-Blot-NB300-307-img0001.jpg "Western Blot: Tryptophan Hydroxylase 1 [p Ser260] Antibody [NB300-307] - Rat brainstem lysate showing specific immunolabeling of the ~55k TPH phosphorylated at Ser260. The labeling by Anti-Ser260TPH is specifically blocked by the Ser260 phosphopeptide (Phos-pep) used as antigen. The corresponding nonphosphopeptide (Nonphos-pep) did not block the immunolabeling.")
![Western Blot: Tryptophan Hydroxylase 1 [p Ser58] Antibody [NB300-260] - Recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of cAMP-dependent protein kinase (PKA) showing specific immunolabeling of the ~53k tryptophan hydroxylase protein phosphorylated at Ser58.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-[p-Ser58]-Antibody-Western-Blot-NB300-260-img0002.jpg "Western Blot: Tryptophan Hydroxylase 1 [p Ser58] Antibody [NB300-260] - Recombinant tryptophan hydroxylase incubated in the absence (Control) and presence of cAMP-dependent protein kinase (PKA) showing specific immunolabeling of the ~53k tryptophan hydroxylase protein phosphorylated at Ser58.")
![Western Blot: DARPP32 [p Ser137] Antibody [NBP2-29514] - Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Ser137 (Control). Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen (peptide) but not by the corresponding dephosphopeptide (not shown).](http://images.novusbio.com/fullsize/DARPP32-[p-Ser137]-Antibody-Western-Blot-NBP2-29514-img0001.jpg "Western Blot: DARPP32 [p Ser137] Antibody [NBP2-29514] - Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Ser137 (Control). Phosphospecificity is shown in the second lane where immunolabeling is blocked by preadsorption of the phospho-peptide used as antigen (peptide) but not by the corresponding dephosphopeptide (not shown).")
![Western Blot: GluR1 Antibody (RH95) [NBP2-29534] - Rat hippocampal lysate showing specific immunolabeling of the ~105k GluR1 protein.](http://images.novusbio.com/fullsize/GluR1-Antibody-RH95-Western-Blot-NBP2-29534-img0002.jpg "Western Blot: GluR1 Antibody (RH95) [NBP2-29534] - Rat hippocampal lysate showing specific immunolabeling of the ~105k GluR1 protein.")
![Western Blot: NMDA R, NR2C Subunit [p Ser1096] Antibody [NBP2-29516] - Western blot of mouse brain lysate showing specific immunolabeling of the ~140k NR2C subunit of the NMDA receptor phosphorylated at Ser1096. The phosphospecificity is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen but not by the dephosphopeptide (not shown).](http://images.novusbio.com/fullsize/NMDA-R--NR2C-Subunit-[p-Ser1096]-Antibody-Western-Blot-NBP2-29516-img0002.jpg "Western Blot: NMDA R, NR2C Subunit [p Ser1096] Antibody [NBP2-29516] - Western blot of mouse brain lysate showing specific immunolabeling of the ~140k NR2C subunit of the NMDA receptor phosphorylated at Ser1096. The phosphospecificity is shown in the second lane where immunoreactivity is blocked by preadsorption with the phospho-peptide (Peptide) used as antigen but not by the dephosphopeptide (not shown).")
![Western Blot: SNAP25 [p Ser187] Antibody [NBP2-29522] - Western blot of rat hippocampal lysate showing specific immunolabeling of the ~25k SNAP25 phosphorylated at Ser187 (Control). Phosphospecificity is shown in the right lane where the signal is completely eliminated by treatment with lambda phosphatase (lambda-Ptase, 400 units/100ul lysate for 30 min).](http://images.novusbio.com/fullsize/SNAP25-[p-Ser187]-Antibody-Western-Blot-NBP2-29522-img0001.jpg "Western Blot: SNAP25 [p Ser187] Antibody [NBP2-29522] - Western blot of rat hippocampal lysate showing specific immunolabeling of the ~25k SNAP25 phosphorylated at Ser187 (Control). Phosphospecificity is shown in the right lane where the signal is completely eliminated by treatment with lambda phosphatase (lambda-Ptase, 400 units/100ul lysate for 30 min).")
![Western Blot: GABA-B R1 [p Ser923] Antibody [NB100-61102] - Western Blot: GABA-B R1 [p Ser923] Antibody [NB100-61102] - Rat synaptic membrane showing specific immune labeling of the ~102k GABA-B R1 [pt Ser923[ (control). The phosphor specificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to theto the p-Ser923 GABA-B antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/GABA-B-R1-[p-Ser923]-Antibody-Western-Blot-NB100-61102-img0006.jpg "Western Blot: GABA-B R1 [p Ser923] Antibody [NB100-61102] - Western Blot: GABA-B R1 [p Ser923] Antibody [NB100-61102] - Rat synaptic membrane showing specific immune labeling of the ~102k GABA-B R1 [pt Ser923[ (control). The phosphor specificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to theto the p-Ser923 GABA-B antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.")