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![Western Blot: GABA-A R beta 2 Antibody [NB300-198] - 7 ug of rat cerebellum (Cb) showing specific immunolabeling of the ~55k beta2-subunit of the GABA-AR.](http://images.novusbio.com/fullsize/GABA-A-R-beta-2-Antibody-Western-Blot-NB300-198-img0004.jpg "Western Blot: GABA-A R beta 2 Antibody [NB300-198] - 7 ug of rat cerebellum (Cb) showing specific immunolabeling of the ~55k beta2-subunit of the GABA-AR.")
![Western Blot: GABA-AR alpha 1 Antibody [NB300-207] - Mouse forebrain lysates from wild type (Control) and a1-knockout (a1-K/O) animals showing specific immunolabeling of the ~51k a1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from a1-knockout animals.](http://images.novusbio.com/fullsize/GABA-AR-alpha-1-Antibody-Western-Blot-NB300-207-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GABA-AR alpha 1 Antibody [NB300-207] - Mouse forebrain lysates from wild type (Control) and a1-knockout (a1-K/O) animals showing specific immunolabeling of the ~51k a1-subunit of the GABAA-R. The labeling was absent from a lysate prepared from a1-knockout animals.")
![Western Blot: GABA-A R beta 1 Antibody [NB300-197] - Mouse whole brain lysates showing specificimmunolabeling of the ~55 kDa beta1-subunit of the GABAA-R.](http://images.novusbio.com/fullsize/GABA-A-R-beta-1-Antibody-Western-Blot-NB300-197-img0005.jpg "Western Blot: GABA-A R beta 1 Antibody [NB300-197] - Mouse whole brain lysates showing specificimmunolabeling of the ~55 kDa beta1-subunit of the GABAA-R.")
![Western Blot: GABA-A R beta 1 Antibody [NB300-197] - Rat cerebellar (Cb) and hippocampal (Hipp) lysates showing specific immunolabeling of the ~55k beta 1-subunit of the GABA-A R beta 1 Antibody.](http://images.novusbio.com/fullsize/GABA-A-R-beta-1-Antibody-Western-Blot-NB300-197-img0004.jpg)
![Western Blot: DARPP32 Antibody [NB300-304] - Detection of DARPP protein in rat hippocampal lysate using NB 300-304.](http://images.novusbio.com/fullsize/DARPP32-Antibody-Western-Blot-NB300-304-img0001.jpg "Western Blot: DARPP32 Antibody [NB300-304] - Detection of DARPP protein in rat hippocampal lysate using NB 300-304.")
![Immunocytochemistry/Immunofluorescence: DARPP-32 Antibody [NB300-304] - Staining of cultured CD1 mouse striatal cells showing specific labeling of DARPP-32 in green and Tuj1 in red. Cells and photo courtesy of QBMCellScience.](http://images.novusbio.com/fullsize/DARPP-32-Antibody-Immunocytochemistry-Immunofluorescence-NB300-304-img0003.jpg)
![Western Blot: GABARAP Antibody [NB300-158] - Analysis of GABARAP in brain lysate using this antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/GABARAP-Antibody-Western-Blot-NB300-158-img0002.jpg "Western Blot: GABARAP Antibody [NB300-158] - Analysis of GABARAP in brain lysate using this antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: mGluR8 Antibody [NLS925] - Anti-GRM8 / MGLUR8 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/mGluR8-Antibody-Immunohistochemistry-Paraffin-NLS925-img0005.jpg "Immunohistochemistry-Paraffin: mGluR8 Antibody [NLS925] - Anti-GRM8 / MGLUR8 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: mGluR8 Antibody [NLS925] - Analysis of anti-GRM8 / MGLUR8 antibody with brain, neurons.](http://images.novusbio.com/fullsize/mGluR8-Antibody-Immunohistochemistry-Paraffin-NLS925-img0002.jpg)
![Immunohistochemistry-Paraffin: mGluR8 Antibody [NLS925] - Analysis of anti-GRM8 / MGLUR8 antibody with human brain, neurons and glia at 7 ug/ml](http://images.novusbio.com/fullsize/mGluR8-Antibody-Immunohistochemistry-Paraffin-NLS925-img0004.jpg)
![Immunohistochemistry-Paraffin: mGluR4 Antibody [NLS4713] - Anti-MGLUR4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 19 ug/ml.](http://images.novusbio.com/fullsize/mGluR4-Antibody-Immunohistochemistry-Paraffin-NLS4713-img0002.jpg "Immunohistochemistry-Paraffin: mGluR4 Antibody [NLS4713] - Anti-MGLUR4 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 19 ug/ml.")
![Immunohistochemistry-Paraffin: mGluR4 Antibody [NLS4713] - Analysis of anti-MGLUR4 antibody with human brain, cortex, neurons at dilution 19 ug/ml.](http://images.novusbio.com/fullsize/mGluR4-Antibody-Immunohistochemistry-Paraffin-NLS4713-img0001.jpg)
![Immunohistochemistry-Paraffin: mGluR8 Antibody [NLS922] - Anti-GRM8 / MGLUR8 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 6 ug/ml.](http://images.novusbio.com/fullsize/mGluR8-Antibody-Immunohistochemistry-Paraffin-NLS922-img0002.jpg "Immunohistochemistry-Paraffin: mGluR8 Antibody [NLS922] - Anti-GRM8 / MGLUR8 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 6 ug/ml.")
![Immunohistochemistry-Paraffin: mGluR8 Antibody [NLS922] - Analysis of anti-GRM8 / MGLUR8 antibody with human brain, cortex at dilution 6 ug/ml.](http://images.novusbio.com/fullsize/mGluR8-Antibody-Immunohistochemistry-Paraffin-NLS922-img0001.jpg)
![Western Blot: iNOS Antibody (PSH03-69) [NBP3-32473] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32473) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32473) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-278202415295526.jpg "Western Blot: iNOS Antibody (PSH03-69) [NBP3-32473] - Western blot analysis of iNOS on different lysates with Rabbit anti-iNOS antibody (NBP3-32473) at 1/1,000 dilution. Lane 1: RAW264.7 cell lysate Lane 2: RAW264.7 treated with 1ug/mL LPS for 24 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 131 kDa Observed band size: 131 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32473) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: iNOS Antibody (PSH03-69) [NBP3-32473] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-iNOS antibody (NBP3-32473) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32473) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-298202414304151.jpg)
![Immunocytochemistry/ Immunofluorescence: iNOS Antibody (PSH03-69) [NBP3-32473] - Immunocytochemistry analysis of RAW264.7 cells treated with or without 1μg/mL LPS for 6 hours labeling iNOS with Rabbit anti-iNOS antibody (NBP3-32473) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-iNOS antibody (NBP3-32473) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32473_rabbit-inos-mab-psh03-69-29202410334018.jpg)
![Immunohistochemistry-Frozen: Nitrotyrosine Antibody (EM-30) [NBP1-96130] - Human tumoral colon epithelium with anti-nitrotyrosine antibody (EM-30).](http://images.novusbio.com/fullsize/Nitrotyrosine-Antibody-EM-30-Immunohistochemistry-Frozen-NBP1-96130-img0001.jpg "Immunohistochemistry-Frozen: Nitrotyrosine Antibody (EM-30) [NBP1-96130] - Human tumoral colon epithelium with anti-nitrotyrosine antibody (EM-30).")
![Immunohistochemistry-Paraffin: NPY4R Antibody [NLS1069] - Anti-NPY4R / PPYR1 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/NPY4R-Antibody-Immunohistochemistry-Paraffin-NLS1069-img0004.jpg "Immunohistochemistry-Paraffin: NPY4R Antibody [NLS1069] - Anti-NPY4R / PPYR1 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: NPY4R Antibody [NLS1069] - Analysis of anti-NPY4R antibody with brain at 4 ug/ ml.](http://images.novusbio.com/fullsize/NPY4R-Antibody-Immunohistochemistry-Paraffin-NLS1069-img0002.jpg)
![Immunohistochemistry-Paraffin: NPY4R Antibody [NLS1069] - Analysis of anti-NPY4R antibody with human brain.](http://images.novusbio.com/fullsize/NPY4R-Antibody-Immunohistochemistry-Paraffin-NLS1069-img0001.jpg)
![Immunohistochemistry-Paraffin: NPY4R Antibody [NLS1069] - Analysis of anti-NPY4R / PPYR1 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/NPY4R-Antibody-Immunohistochemistry-Paraffin-NLS1069-img0003.jpg)
![Western Blot: CaMKIV Antibody [NBP1-78002] - Shows detection of a band ~52 kDa corresponding to CaM Kinase IV (arrowhead) in various preparations: lane 1 - rat brain lysate, lane 2 - Jurkat cell lysate. Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively). Approximately 35 ug of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase IV was similarly detected on lysates from mouse brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1,000 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.](http://images.novusbio.com/fullsize/CaMKIV-Antibody-Western-Blot-NBP1-78002-img0003.jpg "Western Blot: CaMKIV Antibody [NBP1-78002] - Shows detection of a band ~52 kDa corresponding to CaM Kinase IV (arrowhead) in various preparations: lane 1 - rat brain lysate, lane 2 - Jurkat cell lysate. Specific reactivity is blocked in both lysates when antibody is preincubated with immunizing peptide (lanes 3 and 4 respectively). Approximately 35 ug of each lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. CaM Kinase IV was similarly detected on lysates from mouse brain (not shown). After blocking the membrane was probed with the primary antibody diluted to 1:1,000 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.")
![Immunohistochemistry-Paraffin: CaMKIV Antibody [NBP1-78002] - Antibody was diluted 1:500 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/CaMKIV-Antibody-Immunohistochemistry-Paraffin-NBP1-78002-img0002.jpg)
