Non-species specific IgG2b Isotype Control (PLRV219)

• Reactivity: All-NA, Hu(-), Mu(-), Po(-), Rt(-)
• Applications: WB, ELISA, Flow, ICC/IF, IHC, IP
• Clone: PLRV219
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Non-species specific IgG2b Isotype Control (PLRV219) Summary

• Description: This Mouse IgG2B (PLRV219) immunoglobulin is useful as an isotype control for Mouse IgG2B antibody.
• Specificity: This mouse IgG2b monoclonal antibody (clone PLRV219) reacts with undefined epitope on a plant pathogen.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified
• Endotoxin Note: Endotoxin level < 0.1 EU/ug.

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Useful as an isotype-matched control in WB, IP, IHC-P, IHC-Fr and IF (surface or intracellular) for Flow. Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Non-species specific IgG2b Isotype Control (PLRV219)

• IgG2B

Background

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. In general, non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.