| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-266SS | |||
| Azide and BSA Free | NBP2-80869 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92717SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73072 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48575-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48504-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41103-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44499 | |||
| BSA Free | NBP2-59775-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89773-25ul | |||
![Immunohistochemistry: Nestin Antibody (10C2) [NB300-266] - GFAP and Nestin immunoreactivity in retinal sections.Epifluorescent acquisition of p28 Reeler and WT retinas. (A-D) As compared to WT, both GFAP (red) and Nestin (green) immunoreactivities were highly visible in Reeler retinas (AB, GCL). Arrows point at yellow immunoreactivity in the white frame (B) indicating GFAP and Nestin co-expression in cells having long-filaments (activated Muller cells), as compared to wild type (arrowheads in A). Nuclei were DAPI counterstained (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212732) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunohistochemistry-NB300-266-img0020.jpg "Immunohistochemistry: Nestin Antibody (10C2) [NB300-266] - GFAP and Nestin immunoreactivity in retinal sections.Epifluorescent acquisition of p28 Reeler and WT retinas. (A-D) As compared to WT, both GFAP (red) and Nestin (green) immunoreactivities were highly visible in Reeler retinas (AB, GCL). Arrows point at yellow immunoreactivity in the white frame (B) indicating GFAP and Nestin co-expression in cells having long-filaments (activated Muller cells), as compared to wild type (arrowheads in A). Nuclei were DAPI counterstained (blue). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212732) licensed under a CC-BY license.")
![Western Blot: Nestin Antibody (10C2) [NB300-266] - WB analysis of 5 ug lysate from (1) Rat's total brain tissue, and (2) human CNS progenitor cells using Nestin antibody (clone 10C2) at 1:1000 dilution. The antibody did not react with rat, whereas, in lysate from human samples, it generated a doublet band representing the phosphorylated and the non-phosphorylated forms of Nestin protein.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Western-Blot-NB300-266-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) [NB300-266] - Analysis of formalin fixed, 0.1% Triton X-100 permeabilized F9 cells (Mouse teratocarcinoma) using Nestin antibody (clone 10C2) at 1:50 dilution. The primary antibody was detected using Alexa Fluor 488 labeled donkey anti-mouse IgG (H+L) cross-adsorbed secondary antibody (green) and nuclei were counterstained with DAPI (blue). An isotype control set was inclubated with mouse IgG1 isotype control antibody and the above mentioned secondary antibody. Nestin antibody (clone 10C2) generated a specific membrane-cytoplasmic staining of Nestin while the isotype control samples did not show any staining under same assay conditions.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunocytochemistry-Immunofluorescence-NB300-266-img0015.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody (10C2) [NB300-266] - Nestin was detected in immersion fixed paraffin-embedded sections of human kidney using anti-human mouse monoclonal antibody (Catalog # NB300-266) at 1:500 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue).Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunohistochemistry-Paraffin-NB300-266-img0013.jpg)
![Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using Nestin antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Flow-Cytometry-NB300-266-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (10C2) [NB300-266] - Visualization of Nestin in PC-3 cells using NB300-266. Courtesy of Dolnick Lab, Roswell Park Cancer Institute.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Immunocytochemistry-Immunofluorescence-NB300-266-img0006.jpg)
![Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - An intracellular stain was performed on SH-SY5Y cells with Nestin [10C2] Antibody NB300-266C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Flow-Cytometry-NB300-266-img0017.jpg)
![Flow Cytometry: Nestin Antibody (10C2) [NB300-266] - An intracellular stain was performed on HeLa cells with Nestin Antibody (10C2)NB300-266PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/Nestin-Antibody-10C2-Flow-Cytometry-NB300-266-img0016.jpg)
![Immunohistochemistry: Nestin Antibody (10C2) - BSA Free [NB300-266] - Regorafenib inhibits tumour growth and vascularisation in xenograft mice. GC1 were implanted into the right forebrain of mice. The mice then received daily oral vehicle or regorafenib at 30 mg/kg for 45 days. Representative photographs of mice brain tumours treated with regorafenib (right panel) or without regorafenib (left panel) stained by hemalun-eosin (median panel) or with nestin antibody (upper and lower panel). The upper panel shows whole brains of mice stained with nestin antibody. The tumour areas were circled in blue, and each surface was measured in mm^2. The red rectangles indicate the zone of the tumour shown in median (hemalun-eosin staining) and lower (nestin IHC) panel. Scale bars, upper panel 2.5 mm, median and lower panel 100 um. The graph shows the tumour size in mm^2Â (expressed as the mean +/- SEM of 4 mice). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35326702/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb300-266_mouse-monoclonal-nestin-antibody-10c2-20920231412574.jpg)
![Immunohistochemistry: Nestin Antibody (10C2) - BSA Free [NB300-266] - Regorafenib inhibits tumour growth & vascularisation in xenograft mice. GC1 were implanted into the right forebrain of mice. The mice then received daily oral vehicle or regorafenib at 30 mg/kg for 45 days. (A) Representative photographs of mice brain tumours treated with regorafenib (right panel) or without regorafenib (left panel) stained by hemalun–eosin (median panel) or with nestin antibody (upper & lower panel). The upper panel shows whole brains of mice stained with nestin antibody. The tumour areas were circled in blue, & each surface was measured in mm2. The red rectangles indicate the zone of the tumour shown in median (hemalun–eosin staining) & lower (nestin IHC) panel. Scale bars, upper panel 2.5 mm, median & lower panel 100 µm. The graph shows the tumour size in mm2 (expressed as the mean ± SEM of 4 mice). (B) Representative immunohistochemistry photographs of CD31+ vessels in brain tumour areas of 3 different mice treated with regorafenib (right panel) or without regorafenib (control, left panel). Arrows indicate CD31+ blood vessels. Scale bars, 20 µm. The graph shows the number of CD31+ vessels in tumours per mm2 (expressed as mean ± SEM of 4 mice). * p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35326702), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-266_mouse-monoclonal-nestin-antibody-10c2-310202416212379.jpg)
![Western Blot: Nestin Antibody (4D11) [NBP1-92717] - Analysis analysis of tissue and cell lysates with mouse mAB against nestin, NBP1-92717, dilution 1:500 in green: [1] protein standard, [2] embryonic E18 rat brain, [3] C6 rat glioma cells, and [4] SH-SY5Y human neuroblastoma cells.](http://images.novusbio.com/fullsize/Nestin-Antibody-4D11-Western-Blot-NBP1-92717-img0006.jpg "Western Blot: Nestin Antibody (4D11) [NBP1-92717] - Analysis analysis of tissue and cell lysates with mouse mAB against nestin, NBP1-92717, dilution 1:500 in green: [1] protein standard, [2] embryonic E18 rat brain, [3] C6 rat glioma cells, and [4] SH-SY5Y human neuroblastoma cells.")
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (4D11) [NBP1-92717] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to nestin, NBP1-92717, dilution 1:500 in red, and costained with chicken pAb to MAP2, dilution 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.](http://images.novusbio.com/fullsize/Nestin-Antibody-4D11-Immunocytochemistry-Immunofluorescence-NBP1-92717-img0005.jpg)
![Immunohistochemistry: Nestin Antibody (4D11) [NBP1-92717] - Staining in mouse cortex microslice section (from postnatal day 3). IHC image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Nestin-Antibody-4D11-Immunohistochemistry-NBP1-92717-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (4D11) [NBP1-92717] - Mixed cultures of neonatal rat neurons and glia stained with NBP1-92717 (red), chicken antibody to Vimentin (NB300-223, green) and DNA (DAPI stain, blue). Astrocytes and neuronal stem cells stain strongly and specifically in a clearly filamentous fashion with the Nestin (4D11) antibody. The filamentous staining pattern is as expected as both Nestin and Vimentin are components of 10nm filaments. Note that some cells contain Nestin, but do not stain strongly for Vimentin and so appear red. Others contain Vimentin and not Nestin and so appear green - these are likely to be fibroblastic or endothelial cells. Some cells express both proteins and so appear yellowish.](http://images.novusbio.com/fullsize/Nestin-Antibody-4D11-Immunocytochemistry-Immunofluorescence-NBP1-92717-img0004.jpg)
![Western Blot: NT-4 Antibody (2E8) [NBP1-47897] - Neurotrophin 4 antibody (2E8) at 1:5000 + Recombinant Human Neurotrophin 4.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Western-Blot-NBP1-47897-img0028.jpg "Western Blot: NT-4 Antibody (2E8) [NBP1-47897] - Neurotrophin 4 antibody (2E8) at 1:5000 + Recombinant Human Neurotrophin 4.")
![Immunocytochemistry/Immunofluorescence: NT-4 Antibody (2E8) [NBP1-47897] - Staining of A549 cells using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Immunocytochemistry-Immunofluorescence-NBP1-47897-img0015.jpg)
![Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Human Kidney tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Immunohistochemistry-Paraffin-NBP1-47897-img0024.jpg)
![Western Blot: NT-4 Antibody (2E8) [NBP1-47897] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NTF4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTF4.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Western-Blot-NBP1-47897-img0027.jpg)
![Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Immunohistochemistry-Paraffin-NBP1-47897-img0016.jpg)
![Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Immunohistochemistry-Paraffin-NBP1-47897-img0017.jpg)
![Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Immunohistochemistry-Paraffin-NBP1-47897-img0018.jpg)
![Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Immunohistochemistry-Paraffin-NBP1-47897-img0020.jpg)
![Immunohistochemistry-Paraffin: NT-4 Antibody (2E8) [NBP1-47897] - Staining of paraffin-embedded Human colon tissue using anti-NTF4 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/NT-4-Antibody-2E8-Immunohistochemistry-Paraffin-NBP1-47897-img0022.jpg)
![Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of TrkA on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Western-Blot-NBP2-67473-img0010.jpg "Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of TrkA on rat brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473] - Staining TrkA in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunocytochemistry-Immunofluorescence-NBP2-67473-img0008.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of paraffin-embedded mouse liver tissue using anti-TrkA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunohistochemistry-Paraffin-NBP2-67473-img0006.jpg)
![Flow Cytometry: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of SH-SY-5Y cells with TrkA antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Flow-Cytometry-NBP2-67473-img0001.jpg)
![Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of TrkA on different lysates using anti-TrkA antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse brain Lane 2: SH-SY-5Y](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Western-Blot-NBP2-67473-img0007.jpg)
![Western Blot: TrkA Antibody (JJ084-04) [NBP2-67473] - Western blot analysis of TrkA on N2A cell lysates using anti-TrkA antibody at 1/500 dilution.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Western-Blot-NBP2-67473-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473] - Staining TrkA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunocytochemistry-Immunofluorescence-NBP2-67473-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TrkA Antibody (JJ084-04) [NBP2-67473] - Staining TrkA in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunocytochemistry-Immunofluorescence-NBP2-67473-img0003.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of paraffin-embedded human tonsil tissue using anti-TrkA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunohistochemistry-Paraffin-NBP2-67473-img0004.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody (JJ084-04) [NBP2-67473] - Analysis of paraffin-embedded mouse kidney tissue using anti-TrkA antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/TrkA-Antibody-JJ084-04-Immunohistochemistry-Paraffin-NBP2-67473-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Analysis of beta-3 Adrenergic R/ADRB3 Antibody with Adipose tissue.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Immunohistochemistry-Paraffin-NLS4198-img0008.jpg "Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Analysis of beta-3 Adrenergic R/ADRB3 Antibody with Adipose tissue.")
![Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Analysis of anti-ADRB3 antibody with human adipose.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Immunohistochemistry-Paraffin-NLS4198-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Human Brain, Glioblastoma tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Immunohistochemistry-Paraffin-NLS4198-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Human Ovary, Carcinoma tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Immunohistochemistry-Paraffin-NLS4198-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Human small intestine tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Immunohistochemistry-Paraffin-NLS4198-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Human prostate tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Immunohistochemistry-Paraffin-NLS4198-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-3 Adrenergic R/ADRB3 Antibody [NLS4198] - Analysis of beta-3 Adrenergic R/ADRB3 Antibody with adrenal tissue.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Immunohistochemistry-Paraffin-NLS4198-img0007.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human heart muscle, kidney, liver and lymph node using Anti-NES antibody NBP2-48575 (A) shows similar protein distribution across tissues to independent antibody NBP2-48504 (B).](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0010.jpg)
![Western Blot: Nestin Antibody [NBP2-48575] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line SK-MEL-30](http://images.novusbio.com/fullsize/Nestin-Antibody-Western-Blot-NBP2-48575-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Nestin Antibody [NBP2-48575] - Staining of human cell line AF22 shows localization to intermediate filaments. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48575-img0009.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human kidney using Anti-NES antibody NBP2-48575.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0013.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0006.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0011.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0012.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48575] - Staining of human liver using Anti-NES antibody NBP2-48575.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48575-img0014.jpg)
![Western Blot: Nestin Antibody [NBP2-48504] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NES antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Nestin-Antibody-Western-Blot-NBP2-48504-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Nestin Antibody [NBP2-48504] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NES antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human liver using Anti-NES antibody NBP2-48504.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0014.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human kidney shows distinct positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0005.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0008.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0010.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0011.jpg)
![Western Blot: Melanocortin-4 R Antibody [NBP2-41103] - Western blot analysis of MC4R in rat brain tissue lysate with MC4R antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/Melanocortin-4-R-Antibody-Western-Blot-NBP2-41103-img0001.jpg "Western Blot: Melanocortin-4 R Antibody [NBP2-41103] - Western blot analysis of MC4R in rat brain tissue lysate with MC4R antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunohistochemistry-Paraffin: Melanocortin-4 R Antibody [NBP2-41103] - human brain tissue with MC4R antibody at 2.5 ug/mL.](http://images.novusbio.com/fullsize/Melanocortin-4-R-Antibody-Immunohistochemistry-Paraffin-NBP2-41103-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Melanocortin-4 R Antibody [NBP2-41103] - Human Brain cells with MC4R antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/Melanocortin-4-R-Antibody-Immunofluorescence-NBP2-41103-img0003.jpg)
![Immunohistochemistry: Melanocortin-4 R Antibody - BSA Free [NBP2-41103] - Immunohistochemistry of Melanocortin-4 R in human brain tissue with Melanocortin-4 R antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41103_rabbit-polyclonal-melanocortin-4-r-antibody-271120242022912.jpg)
![Immunocytochemistry/ Immunofluorescence: Melanocortin-4 R Antibody - BSA Free [NBP2-41103] - Immunofluorescence of Melanocortin-4 R in Human Brain cells with Melanocortin-4 R antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp2-41103_rabbit-polyclonal-melanocortin-4-r-antibody-2711202419562138.jpg)
![Immunohistochemistry: Melanocortin-4 R Antibody - BSA Free [NBP2-41103] - Immunohistochemistry of Melanocortin-4 R in human brain tissue with Melanocortin-4 R antibody at 2.5 u/mL.](http://images.novusbio.com/fullsize/nbp2-41103_rabbit-polyclonal-melanocortin-4-r-antibody-2711202419531688.jpg)
![Western Blot: beta-3 Adrenergic R/ADRB3 Antibody [H00000155-B01P] - Analysis of ADRB3 expression in transfected 293T cell line by ADRB3 polyclonal antibody. Lane 1: ADRB3 transfected lysate(44.88 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Western-Blot-H00000155-B01P-img0005.jpg "Western Blot: beta-3 Adrenergic R/ADRB3 Antibody [H00000155-B01P] - Analysis of ADRB3 expression in transfected 293T cell line by ADRB3 polyclonal antibody. Lane 1: ADRB3 transfected lysate(44.88 KDa). Lane 2: Non-transfected lysate.")
![Flow Cytometry: beta-3 Adrenergic R/ADRB3 Antibody [H00000155-B01P] - Analysis of negative control 293 cells (Black) and ADRB3 expressing 293 cells (Green) using ADRB3 purified MaxPab mouse polyclonal antibody.](http://images.novusbio.com/fullsize/beta-3-Adrenergic-R-ADRB3-Antibody-Flow-Cytometry-H00000155-B01P-img0003.jpg)
![Western Blot: Nestin Antibody (CL0197) [NBP2-59775] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/Nestin-Antibody-CL0197-Western-Blot-NBP2-59775-img0004.jpg "Western Blot: Nestin Antibody (CL0197) [NBP2-59775] - Lane 1: Marker [kDa] Lane 2: Human cell line U-251 MG")
![Immunocytochemistry/Immunofluorescence: Nestin Antibody (CL0197) [NBP2-59775] - Staining of RH-30 cells using the Anti-NES monoclonal antibody, showing specific staining in intermediate filaments in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/Nestin-Antibody-CL0197-Immunocytochemistry-Immunofluorescence-NBP2-59775-img0005.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775] - Staining of human kidney shows strong positivity in renal glomeruli.](http://images.novusbio.com/fullsize/Nestin-Antibody-CL0197-Immunohistochemistry-Paraffin-NBP2-59775-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Nestin Antibody (CL0197) [NBP2-59775] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-NES monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/Nestin-Antibody-CL0197-Western-Blot-NBP2-59775-img0003.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody (CL0197) [NBP2-59775] - Staining of human cerebral cortex shows strong immunoreactivity in the endothelial cells.](http://images.novusbio.com/fullsize/Nestin-Antibody-CL0197-Immunohistochemistry-Paraffin-NBP2-59775-img0001.jpg)
![Western Blot: FTO Antibody (2Q9K10) [NBP3-16285] - Analysis of extracts of various cell lines, using FTO Rabbit mAb (NBP3-16285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/FTO-Antibody-2Q9K10-Western-Blot-NBP3-16285-img0003.jpg "Western Blot: FTO Antibody (2Q9K10) [NBP3-16285] - Analysis of extracts of various cell lines, using FTO Rabbit mAb (NBP3-16285) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: FTO Antibody (2Q9K10) [NBP3-16285] - Immunofluorescence analysis of U-2 OS cells using FTO Rabbit mAb (NBP3-16285) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FTO-Antibody-2Q9K10-Immunocytochemistry-Immunofluorescence-NBP3-16285-img0002.jpg)
![Immunohistochemistry-Paraffin: FTO Antibody (2Q9K10) [NBP3-16285] - Human bladder cancer using FTO Rabbit mAb (NBP3-16285) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/FTO-Antibody-2Q9K10-Immunohistochemistry-Paraffin-NBP3-16285-img0001.jpg)
![Immunoprecipitation: FTO Antibody (2Q9K10) [NBP3-16285] - Analysis of 300ug extracts of 293T cells using 3ug FTO antibody (NBP3-16285). Western blot was performed from the immunoprecipitate using FTO antibody (NBP3-16285) at a dilition of 1:500.](http://images.novusbio.com/fullsize/FTO-Antibody-2Q9K10-Immunoprecipitation-NBP3-16285-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: FTO Antibody (2Q9K10) [NBP3-16285] - Confocal imaging of U-2 OS cells using FTO Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16285_rabbit-fto-mab-2q9k10-52202511383732.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody [NBP1-89773] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Ghrelin-Obestatin-Antibody-Immunohistochemistry-Paraffin-NBP1-89773-img0014.jpg)
![Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody [NBP1-89773] - Staining of human colon shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Ghrelin-Obestatin-Antibody-Immunohistochemistry-Paraffin-NBP1-89773-img0012.jpg)
![Immunohistochemistry-Paraffin: Ghrelin/Obestatin Antibody [NBP1-89773] - Staining of human duodenum shows weak cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Ghrelin-Obestatin-Antibody-Immunohistochemistry-Paraffin-NBP1-89773-img0013.jpg)
