| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-148SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76798SS | |||
| Azide and BSA Free | NBP2-80879 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB400-140SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92925-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50194-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54678-25ul | |||
![Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - NPC proteins in mouse lungs. Western blot of wild type (W) littermates, NPC1 (Mut) or NPC2 (Mut) mutant mouse lungs using anti-NPC1 or -NPC2 antibody. beta-actin used as a loading control. 30 ug protein/lane. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0067084), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Western-Blot-NB400-148-img0015.jpg "Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - NPC proteins in mouse lungs. Western blot of wild type (W) littermates, NPC1 (Mut) or NPC2 (Mut) mutant mouse lungs using anti-NPC1 or -NPC2 antibody. beta-actin used as a loading control. 30 ug protein/lane. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0067084), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - NPC1 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Immunocytochemistry-Immunofluorescence-NB400-148-img0010.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Staining of human brain, cortex, neurons and astrocytes.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB400-148-img0011.jpg)
![Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Protein expression analysis of normal and NPC-deficient cells after HIV-1 infection. Cells were uninfected or infected with VSVG-HIV-1 and harvested 96 h post-infection. NPC2, NPC1, and beta-actin protein expression was detected via Western blotting in uninfected and infected cells. Gag expression was also detected in the infected cells. Image collected and cropped by CiteAb from the following publication (//virologyj.biomedcentral.com/articles/10.1186/1743-422X-9-31), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Western-Blot-NB400-148-img0016.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Immunocytochemical staining with corresponding colocalization analysis of ALIX (green) and NPC-1 (red) in fibroblasts exposed to LLOMe for 15 min. Nuclei are stained with DAPI (blue) and merged images show colocalization in yellow (n = 5). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32409651/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Niemann-Pick C1 Antibody - BSA Free-Immunocytochemistry-Immunofluorescence-NB400-148-img0017.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NPC1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human NPC1 Polyclonal Antibody (Catalog # NB400-148) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for NPC1 at approximately 240-260 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Knockout-Validated-NB400-148-img0014.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - HeLa lysate, BHK lysate, A431 lysate tested at 1:1000. Image from verified customer review.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody---BSA-Free-Knockdown-Validated-NB400-148-img0012.jpg)
![Immunocytochemistry/ Immunofluorescence: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - ESCRT complex recruited to LLOMe-damaged lysosomes promotes cell survival.Human fibroblasts were exposed to 1 mM LLOMe & when indicated, the cell permeable calcium chelator BAPTA-AM (1 µM) was added 10 min prior to LLOMe exposure. Immunocytochemical staining with corresponding colocalization analysis of a CHMP4B (green) & LAMP2 (red), b ALIX (green) & NPC-1 (red), & c CHMP4B (green) & LAMP2 (red) in fibroblasts exposed to LLOMe for 15 min. Nuclei are stained with DAPI (blue) & merged images show colocalization in yellow (n = 5). d Caspase-3 like activity in cells exposed to LLOMe for 8 h (n = 3). e Quantification of apoptotic & necrotic cells using Annexin V/PI staining in fibroblasts exposed to LLOMe for 6 h (n = 3). f Immunoblot of LAMP2 in digitonin-extracted cytosolic fractions from cells exposed to LLOMe for 2 h (n = 8) with corresponding quantitative analysis. *p < 0.05, **p < 0.01 & ***p < 0.001 compared to control (a–b) or LLOMe only (c–f). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32409651), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-148_rabbit-polyclonal-niemann-pick-c1-antibody-310202416165630.jpg)
![Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Expression of proteins involved in extracellular lipid uptake & intracellular cholesterol trafficking following JQ1 (0.4 µM) administration to HepG2 cells for 48 hours. (A) Representative Western blot (left panel) & densitometric analysis (right panel) of SR-B1. n = 6 independent experiments. (B) Immunofluorescence analysis of SR-B1 (green). Nuclei were counterstained with DAPI. n = 3 different experiments. Scale bar: 50 µm. (C) Representative Western blot (left panel) & densitometric analysis (right panel) of LDLr. n = 5 independent experiments. (D) LDLr immunofluorescence (green). Nuclei were counterstained with DAPI. n = 3 different experiments. (E–F) Representative Western blots & densitometric analysis of NPC1 & TMEM97. Tubulin was chosen as loading control. n = 6 independent experiments. Data represent means ± SD. Statistical analysis was performed by using unpaired Student’s t test. ** p < 0.01; *** p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32075110), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-148_rabbit-polyclonal-niemann-pick-c1-antibody-310202416163786.jpg)
![Western Blot: Niemann-Pick C1 Antibody - BSA Free [NB400-148] - Effects of p75NTR modulation by LM11A-31 on cholesterol metabolism & secretion. (A–D) Representative Western blot & densitometric analysis of HMGCR, ABCA1, NPC1, & ApoE in U373 cells treated with vehicle (Ctrl) & LM11A-31 (0.1 µM) for 48 h. n = 4 different experiments. Vinculin served as a housekeeping protein to normalize protein loading. (E) Quantification of ApoE amount (µg/mL) by ELISA assay in culture medium from vehicle- & LM11A-31-treated U373 cells. n = 3 different experiments. (F) Cholesterol quantification (total cholesterol, free cholesterol, & cholesteryl esters) in the culture medium of U373 cells treated with vehicle (Ctrl) & LM11A-31 (0.1 µM) for 48 h. n = 3 different experiments. Data represent means ± SD. Statistical analysis was carried out by using unpaired Student’s t test. *** p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35563230), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-148_rabbit-polyclonal-niemann-pick-c1-antibody-31020241616058.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Knockout-Validated-NBP2-76798-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti- Niemann-Pick C1 Antibody at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0006.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Total protein from human HeLa, Hek293, mouse 3T3 and Rat PC12 cell lines were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Western-Blot-NBP2-76798-img0005.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Niemann-Pick Type C1/NPC1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (NBP2-76798) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic puncta.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunohistochemistry-Paraffin-NBP2-76798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Niemann-Pick C1 Antibody (1318A) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0004.jpg)
![Western Blot: CYP46A1 Antibody [NB400-140] - Blocking experiment using NB 400-140 in HEK293 cells overexpressing CYP46A1.](http://images.novusbio.com/fullsize/CYP46A1-Antibody-Western-Blot-NB400-140-img0011.jpg "Western Blot: CYP46A1 Antibody [NB400-140] - Blocking experiment using NB 400-140 in HEK293 cells overexpressing CYP46A1.")
![Immunocytochemistry/Immunofluorescence: CYP46A1 Antibody [NB400-140] - CYP46A1 antibody was tested in SH-SY5Y cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/CYP46A1-Antibody-Immunocytochemistry-Immunofluorescence-NB400-140-img0013.jpg)
![Western Blot: CYP46A1 Antibody [NB400-140] - Detection of Cyp46 in human brain lysates.](http://images.novusbio.com/fullsize/CYP46A1-Antibody-Western-Blot-NB400-140-img0005.jpg)
![Western Blot: CYP46A1 Antibody [NB400-140] - Analysis of Cyp-46 in HEK293 cells overexpressing CYP46A1 or transfected with an empty vector using NB400-140.](http://images.novusbio.com/fullsize/CYP46A1-Antibody-Western-Blot-NB400-140-img0010.jpg)
![Western Blot: CYP46A1 Antibody [NBP2-16066] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16066_rabbit-polyclonal-cyp46a1-antibody-91020242014662.jpg "Western Blot: CYP46A1 Antibody [NBP2-16066] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: CYP46A1 Antibody [NBP2-16066] - CYP46A1 antibody [N1C1] detects CYP46A1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.CYP46A1 stained by CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16066_rabbit-polyclonal-cyp46a1-antibody-910202420104468.jpg)
![Immunohistochemistry-Paraffin: CYP46A1 Antibody [NBP2-16066] - CYP46A1 antibody [N1C1] detects CYP46A1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse hippocampus.CYP46A1 stained by CYP46A1 antibody [N1C1] (NBP2-16066) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-16066_rabbit-polyclonal-cyp46a1-antibody-910202419593547.jpg)
![Western Blot: CYP46A1 Antibody [NBP2-92925] - Analysis of extracts of various cell lines, using CYP46A1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/CYP46A1-Antibody-Western-Blot-NBP2-92925-img0002.jpg "Western Blot: CYP46A1 Antibody [NBP2-92925] - Analysis of extracts of various cell lines, using CYP46A1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Western Blot: CYP46A1 Antibody (N8-P6E4-H8) [NBP2-50194] - The left hand lane contains control cell lysate while the right hand lane contains lysate prepared from cells over-expressing the protein. ~15 micrograms of protein were loaded per well. No bands were detected with the negative control lysate, while a band migrating at the expected molecular weight was observed in the over-expression lysate.](http://images.novusbio.com/fullsize/CYP46A1-Antibody-N8-P6E4-H8-Western-Blot-NBP2-50194-img0002.jpg "Western Blot: CYP46A1 Antibody (N8-P6E4-H8) [NBP2-50194] - The left hand lane contains control cell lysate while the right hand lane contains lysate prepared from cells over-expressing the protein. ~15 micrograms of protein were loaded per well. No bands were detected with the negative control lysate, while a band migrating at the expected molecular weight was observed in the over-expression lysate.")
![Immunohistochemistry: CYP46A1 Antibody (N8-P6E4-H8) [NBP2-50194] - Shows strong cytoplasmic staining in metastatic colorectal cancer (A) and primary colorectal (B) compared to normal colon mucosa (C).](http://images.novusbio.com/fullsize/CYP46A1-Antibody-N8-P6E4-H8-Immunohistochemistry-NBP2-50194-img0001.jpg)
![Western Blot: Niemann-Pick C1 Antibody [NBP2-26214] - Staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-Western-Blot-NBP2-26214-img0001.jpg "Western Blot: Niemann-Pick C1 Antibody [NBP2-26214] - Staining of HEK293 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Recombinant Human CYP46A1 Protein [H00010858-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00010858-Q01-1.jpg "Recombinant Human CYP46A1 Protein [H00010858-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody [NBP2-54678] - Staining of human gall bladder shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-Immunohistochemistry-Paraffin-NBP2-54678-img0001.jpg "Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody [NBP2-54678] - Staining of human gall bladder shows strong cytoplasmic positivity in glandular cells.")
![Western Blot: CYP46A1 Overexpression Lysate (Adult Normal) [NBL1-09694] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CYP46A1.](http://images.novusbio.com/fullsize/CYP46A1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09694-img0002.jpg "Western Blot: CYP46A1 Overexpression Lysate (Adult Normal) [NBL1-09694] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CYP46A1.")