| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48504-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05215-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34413-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47834-0.1mg | |||
| IHC-Prediluted | NBP2-48252 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90342-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48609-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59329-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37525-0.025ml | |||
![Western Blot: Nestin Antibody [NBP2-48504] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NES antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/Nestin-Antibody-Western-Blot-NBP2-48504-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Nestin Antibody [NBP2-48504] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NES antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human liver using Anti-NES antibody NBP2-48504.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0014.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human kidney shows distinct positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0005.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human liver shows low expression as expected.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0008.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0010.jpg)
![Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human heart muscle.](http://images.novusbio.com/fullsize/Nestin-Antibody-Immunohistochemistry-Paraffin-NBP2-48504-img0011.jpg)
![Western Blot: Pannexin-1 Antibody [NBP1-59672] - Human Jurkat cell lysate Antibody Dilution: 1.0 ug/ml.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP1-59672-img0010.jpg "Western Blot: Pannexin-1 Antibody [NBP1-59672] - Human Jurkat cell lysate Antibody Dilution: 1.0 ug/ml.")
![Immunohistochemistry: Pannexin-1 Antibody [NBP1-59672] - Paraffin Embedded Tissue: Human neural cell Cellular Data: Epithelial cells of renal tubule Antibody Concentration: 4.0 - 8.0 ug/ml Magnification: 400X](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-NBP1-59672-img0008.jpg)
![Western Blot: Pannexin-1 Antibody [NBP1-59672] - Tissue: Hela whole cell lysates](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP1-59672-img0004.jpg)
![Western Blot: Pannexin-1 Antibody [NBP1-59672] - PANX1 Antibody Titration: 0.2-1 ug/ml Positive Control: Jurkat cell lysate](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP1-59672-img0001.jpg)
![Western Blot: Pannexin-1 Antibody [NBP1-59672] - Sample Tissue: Human 293T Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Western-Blot-NBP1-59672-img0009.jpg)
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Pannexin-1 Antibody [NBP1-59672] - Mouse Retina and Knockout PANX-1 Mouse Tissue.](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-59672-img0003.jpg)
![Immunohistochemistry: Pannexin-1 Antibody [NBP1-59672] - Human Intestine](http://images.novusbio.com/fullsize/Pannexin-1-Antibody-Immunohistochemistry-NBP1-59672-img0007.jpg)
![Western Blot: NF-H Antibody [NBP1-05215] - Analysis of different tissue lysates using NF-H antibody, dilution 1:10,000 (Green): [1] protein standard (Red), [2] rat brain, [3] rat spinal cord [4] mouse brain, and [5] mouse spinal cord lysate. Strong band at about 220kDa corresponds to the phosphorylated axonal form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected.](http://images.novusbio.com/fullsize/NF-H-Antibody-Western-Blot-NBP1-05215-img0006.jpg "Western Blot: NF-H Antibody [NBP1-05215] - Analysis of different tissue lysates using NF-H antibody, dilution 1:10,000 (Green): [1] protein standard (Red), [2] rat brain, [3] rat spinal cord [4] mouse brain, and [5] mouse spinal cord lysate. Strong band at about 220kDa corresponds to the phosphorylated axonal form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected.")
![Immunocytochemistry/Immunofluorescence: NF-H Antibody [NBP1-05215] - Shows mixed neuron/glial rat brain cultures stained with NBP1-05215 (green) and chicken polyclonal antibody to Glial Fibrillary Acidic Protein (GFAP) NBP1-05198 (red). The neurofilament NF-H antibody binds to phosphorylated axonal forms of NF-H and non-phosphorylated dendritic and perikaryal forms.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-05215-img0004.jpg)
![Immunohistochemistry Free-Floating: NF-H Antibody [NBP1-05215] - Analysis of a mouse hippocampus section stained with NF-H antibody, dilution 1:2,000 (Red), and costained with mouse myelin basic protein mAb (MBP), dilution 1:5,000 (Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with above antibodies. The NF-H antibody labels a network of axons of different neurons, while the MBP antibody stains myelin sheath around these axons.](http://images.novusbio.com/fullsize/NF-H-Antibody-Immunohistochemistry-Free-Floating-NBP1-05215-img0007.jpg)
![Western Blot: NF-H Antibody [NBP1-05215] - Blot of rat spinal cord extract blotted with NBP1-05215. A major band running at 200kDa is NF-H, the major neurofilament subunit protein. A minor band at about 160kDa is the non-phosphorylated dendritic and perilaryal form of this protein.](http://images.novusbio.com/fullsize/NF-H-Antibody-Western-Blot-NBP1-05215-img0005.jpg)
![Western Blot: GFAP Antibody (SPM507) [NBP2-34365] - Western Blot Analysis of human Brain tissue lysate using GFAP Antibody (SPM507).](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM507-Western-Blot-NBP2-34365-img0004.jpg "Western Blot: GFAP Antibody (SPM507) [NBP2-34365] - Western Blot Analysis of human Brain tissue lysate using GFAP Antibody (SPM507).")
![Immunohistochemistry-Paraffin: GFAP Antibody (SPM507) [NBP2-34365] - Formalin-paraffin human brain stained with GFAP Ab (SPM507). Note cytoplasmic staining.](http://images.novusbio.com/fullsize/GFAP-Antibody-SPM507-Immunohistochemistry-Paraffin-NBP2-34365-img0001.jpg)
![Western Blot: NF-H Antibody (NE14) [NBP2-44728] - Western Blot Analysis of human Brain tissue lysate using NF-H Antibody (NE14).](http://images.novusbio.com/fullsize/NF-H-Antibody-NE14-Western-Blot-NBP2-44728-img0005.jpg "Western Blot: NF-H Antibody (NE14) [NBP2-44728] - Western Blot Analysis of human Brain tissue lysate using NF-H Antibody (NE14).")
![Immunohistochemistry-Paraffin: NF-H Antibody (NE14) [NBP2-44728] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NE14).](http://images.novusbio.com/fullsize/NF-H-Antibody-NE14-Immunohistochemistry-Paraffin-NBP2-44728-img0002.jpg)
![Flow Cytometry: NF-H Antibody (NE14) [NBP2-44728] - Flow Cytometric Analysis of HEK293 cells using NF-H Antibody (NE14).followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-H-Antibody-NE14-Flow-Cytometry-NBP2-44728-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-H Antibody (NE14) [NBP2-44728] - Human Cerebellum stained with Neurofilament Monoclonal Antibody (NE14).](http://images.novusbio.com/fullsize/NF-H-Antibody-NE14-Immunohistochemistry-Paraffin-NBP2-44728-img0001.jpg)
![Western Blot: QPRT Antibody (5D11) [H00023475-M01] - QPRT monoclonal antibody (M01), clone 5D11 Analysis of QPRT expression in HepG2.](http://images.novusbio.com/fullsize/QPRT-Antibody-5D11-Western-Blot-H00023475-M01-img0007.jpg "Western Blot: QPRT Antibody (5D11) [H00023475-M01] - QPRT monoclonal antibody (M01), clone 5D11 Analysis of QPRT expression in HepG2.")
![Immunohistochemistry-Paraffin: QPRT Antibody (5D11) [H00023475-M01] - Analysis of monoclonal antibody to QPRT on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/QPRT-Antibody-5D11-Immunohistochemistry-Paraffin-H00023475-M01-img0005.jpg)
![Sandwich ELISA: QPRT Antibody (5D11) [H00023475-M01] - Detection limit for recombinant GST tagged QPRT is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/QPRT-Antibody-5D11-Sandwich-ELISA-H00023475-M01-img0006.jpg)
![Western Blot: Stargazin Antibody (HL2268) - Azide and BSA Free [NBP3-25697] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Stargazin antibody [HL2268] (NBP3-25697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25697_rabbit-stargazin-mab-hl2268-azide-and-bsa-free-2120249413329.jpg "Western Blot: Stargazin Antibody (HL2268) - Azide and BSA Free [NBP3-25697] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Stargazin antibody [HL2268] (NBP3-25697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Stargazin Antibody (HL2268) - Azide and BSA Free [NBP3-25697] - Stargazin antibody [HL2268] detects Stargazin protein at cell membrane and synapse by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Stargazin stained by Stargazin antibody [HL2268] (NBP3-25697) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25697_rabbit-stargazin-mab-hl2268-azide-and-bsa-free-212024939351.jpg)
![Immunohistochemistry-Paraffin: Stargazin Antibody (HL2268) - Azide and BSA Free [NBP3-25697] - Stargazin antibody [HL2268] detects Stargazin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Stargazin stained by Stargazin antibody [HL2268] (NBP3-25697) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25697_rabbit-stargazin-mab-hl2268-azide-and-bsa-free-2120249355571.jpg)
![Immunocytochemistry/Immunofluorescence: Stargazin Antibody (HL2268) - Azide and BSA Free [NBP3-25697] - Stargazin antibody [HL2268] detects Stargazin protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Stargazin stained by Stargazin antibody [HL2268] (NBP3-25697) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25697_rabbit-stargazin-mab-hl2268-azide-and-bsa-free-2120249403837.jpg)
![Western Blot: Stargazin Antibody (HL2268) - Azide and BSA Free [NBP3-25697] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Stargazin antibody [HL2268] (NBP3-25697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25697_rabbit-stargazin-mab-hl2268-azide-and-bsa-free-2120249425417.jpg)
![Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Stargazin antibody [HL2268] (NBP3-25697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](http://images.novusbio.com/fullsize/nbp3-25697_rabbit-stargazin-mab-hl2268-azide-and-bsa-free-western-blot-1411202518314518.jpg)
![Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/nbp1-90342_rabbit-polyclonal-beta-synuclein-antibody-29520251557160.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemical analysis of human placenta and testis tissues. Corresponding SLC2A1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0017.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemical analysis of human placenta and testis tissues. Corresponding SLC2A1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Glut1 Antibody [NBP2-48609] - Immunocytochemistry analysis of human cell line U-251 MG shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48609-img0018.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemical analysis of human cerebral cortex shows strong membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0013.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Staining of human skin shows moderate membranous positivity in epidermal cells.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-NBP2-48609-img0019.jpg)
![Immunohistochemistry: Glut1 Antibody [NBP2-48609] - Immunohistochemistry analysis of mouse brain shows moderate positivity in endothelial cells in the cerebral cortex.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0012.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemistry analysis of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0014.jpg)
![Immunohistochemistry-Paraffin: Glut1 Antibody [NBP2-48609] - Immunohistochemistry analysis of human testis shows no membranous positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Glut1-Antibody-Immunohistochemistry-Paraffin-NBP2-48609-img0016.jpg)
![Western Blot: VGLUT1 Antibody (S28-9) [NBP2-59329] - Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (NBP2-59329). Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (NBP2-59329) at 1:1000.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-S28-9-Western-Blot-NBP2-59329-img0005.jpg "Western Blot: VGLUT1 Antibody (S28-9) [NBP2-59329] - Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (NBP2-59329). Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (NBP2-59329) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: VGLUT1 Antibody (S28-9) [NBP2-59329] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (NBP2-59329). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (NBP2-59329) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-S28-9-Immunocytochemistry-Immunofluorescence-NBP2-59329-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: VGLUT1 Antibody (S28-9) [NBP2-59329] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (NBP2-59329). Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (NBP2-59329) at 1:100. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) VGLUT1 Antibody. (D) Hoechst (E) Composite.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-S28-9-Immunocytochemistry-Immunofluorescence-NBP2-59329-img0007.jpg)
![Western Blot: NF-L Antibody (2G10) [NBP2-37525] - Analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Western-Blot-NBP2-37525-img0002.jpg "Western Blot: NF-L Antibody (2G10) [NBP2-37525] - Analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (2G10) [NBP2-37525] - Analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Immunocytochemistry-Immunofluorescence-NBP2-37525-img0003.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (2G10) [NBP2-37525] - Analysis of brain tissues using NEFL mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Immunohistochemistry-NBP2-37525-img0006.jpg)
![Flow Cytometry: NF-L Antibody (2G10) [NBP2-37525] - Analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Flow-Cytometry-NBP2-37525-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (2G10) [NBP2-37525] - Analysis of lung cancer tissues using NEFL mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Immunohistochemistry-NBP2-37525-img0005.jpg)
![ELISA: NF-L Antibody (2G10) [NBP2-37525] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-ELISA-NBP2-37525-img0001.jpg)
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414262648.jpg "Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glut1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32385, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry: Glut1 Antibody (SA0377) [NBP3-32385] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32385, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-298202414333665.jpg)
![Immunocytochemistry/ Immunofluorescence: Glut1 Antibody (SA0377) [NBP3-32385] - Immunocytochemistry analysis of HeLa cells labeling Glut1 with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/500 dilution.Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202410334092.jpg)
![Flow Cytometry: Glut1 Antibody (SA0377) [NBP3-32385] - Flow cytometric analysis of Jurkat cells labeling Glut1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32385, red) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (blue). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with an iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-29202416372147.jpg)
![Western Blot: Glut1 Antibody (SA0377) [NBP3-32385] - Western blot analysis of Glut1 on different lysates with Rabbit anti-Glut1 antibody (NBP3-32385) at 1/50,000 dilution. Lane 1: HeLa cell lysate (no heat) Lane 2: HT-29 cell lysate (no heat) Lane 3: HepG2 cell lysate (no heat) Lane 4: NIH/3T3 cell lysate (no heat) Lane 5: L-929 cell lysate (no heat) Lane 6: Mouse brain tissue lysate (no heat) Lane 7: Rat brain tissue lysate (no heat) Lysates/proteins at 20 µg/Lane. Predicted band size: 54 kDa Observed band size: 45-60 kDa Exposure time: Lane 1-7 (left): 20 seconds; Lane 1-7 (right): 1 minute 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/50,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32385_rabbit-glut1-mab-sa0377-3920248453511.jpg)