| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74822 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Atto594 | NBP3-14765-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-82375SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25148SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88204-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81768-25ul | |||
![Western Blot: C9orf72 Antibody (779) [NBP3-13507] - C9orf72 Antibody (GT779) [NBP3-13507] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [779] (NBP3-13507) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/C9orf72-Antibody-GT779-Western-Blot-NBP3-13507-img0013.jpg "<b>Genetic Strategies Validation. </b>Western Blot: C9orf72 Antibody (779) [NBP3-13507] - C9orf72 Antibody (GT779) [NBP3-13507] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [779] (NBP3-13507) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse brain. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/C9orf72-Antibody-GT779-Immunohistochemistry-Paraffin-NBP3-13507-img0011.jpg)
![Western Blot: C9orf72 Antibody (GT779) [NBP3-13507] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody (NBP3-13507) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/C9orf72-Antibody-GT779-Western-Blot-NBP3-13507-img0003.jpg)
![Western Blot: C9orf72 Antibody (GT779) [NBP3-13507] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (NBP3-13507) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/C9orf72-Antibody-GT779-Western-Blot-NBP3-13507-img0007.jpg)
![Immunohistochemistry-Paraffin: C9orf72 Antibody (GT779) [NBP3-13507] - Human colon cancer. C9orf72 antibody [779] (NBP3-13507) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/C9orf72-Antibody-GT779-Immunohistochemistry-Paraffin-NBP3-13507-img0002.jpg)
![Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse hippocampus. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/C9orf72-Antibody-GT779-Immunohistochemistry-Paraffin-NBP3-13507-img0004.jpg)
![Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse hippocampus. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/C9orf72-Antibody-GT779-Immunohistochemistry-Paraffin-NBP3-13507-img0005.jpg)
![Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse hippocampus. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/C9orf72-Antibody-GT779-Immunohistochemistry-Paraffin-NBP3-13507-img0006.jpg)
![Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse brain. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/C9orf72-Antibody-GT779-Immunohistochemistry-Paraffin-NBP3-13507-img0009.jpg)
![Immunohistochemistry-Paraffin: C9orf72 Antibody (779) [NBP3-13507] - Mouse brain. C9orf72 stained by C9orf72 antibody [779] (NBP3-13507) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/C9orf72-Antibody-GT779-Immunohistochemistry-Paraffin-NBP3-13507-img0010.jpg)
![Immunoprecipitation: C9orf72 Antibody (779) [NBP3-13507] - C9orf72 Antibody (GT779) [NBP3-13507] - Immunoprecipitation of C9orf72 protein from SK-N-SH whole cell extracts using 5 ug of C9orf72 antibody [779] (NBP3-13507). Western blot analysis was performed using C9orf72 antibody [779] (NBP3-13507). EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/C9orf72-Antibody-GT779-Immunoprecipitation-NBP3-13507-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: C9orf72 Antibody (779) [NBP3-13507] - C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunofluorescent analysis.Sample: DIV10 rat E18 primary hippocampal neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: C9orf72 stained by C9orf72 antibody [GT779] (NBP3-13507) diluted at 1:500.Red: Tau, a Tau marker, stained by Tau antibody diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13507_mouse-c9orf72-mab-gt779-910202420134070.jpg)
![Western Blot: C9orf72 Antibody (779) [NBP3-13507] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (NBP3-13507) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13507_mouse-c9orf72-mab-gt779-910202420134055.jpg)
![Western Blot: USP9x Antibody (2G7) [NBP2-03824] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-USP9x monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Western-Blot-NBP2-03824-img0009.jpg "Western Blot: USP9x Antibody (2G7) [NBP2-03824] Analysis of extracts (35ug) from 9 different cell lines by usin g anti-USP9x monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Human prostate tissue using anti-USP9x mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-03824-img0008.jpg)
![Western Blot: USP9x Antibody (2G7) [NBP2-03824] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY USP9x (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP9x.](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Western-Blot-NBP2-03824-img0001.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-USP9x mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-03824-img0002.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-USP9x mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-03824-img0003.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-USP9x mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-03824-img0004.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-USP9x mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-03824-img0005.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Human Kidney tissue using anti-USP9x mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-03824-img0006.jpg)
![Immunohistochemistry-Paraffin: USP9x Antibody (2G7) [NBP2-03824] - Staining of paraffin-embedded Human liver tissue using anti-USP9x mouse monoclonal antibody.](http://images.novusbio.com/fullsize/USP9x-Antibody-2G7-Immunohistochemistry-Paraffin-NBP2-03824-img0007.jpg)
![Western Blot: HSP27 Antibody [NB120-5579] - Analysis of 50ug of the indicated whole cell lysates per well.](http://images.novusbio.com/fullsize/HSP27-Antibody-Western-Blot-NB120-5579-img0003.jpg "Western Blot: HSP27 Antibody [NB120-5579] - Analysis of 50ug of the indicated whole cell lysates per well.")
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in MCF-7 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0007.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NB120-5579] - Both normal and cancer biopsies of deparaffinized human Breast carcinoma tissue.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunohistochemistry-Paraffin-NB120-5579-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with HSP27 polyclonal antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in C6 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody [NB120-5579] - Analysis of Heat Shock Protein 27 in HeLa Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a Heat Shock Protein 27 polyclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Heat Shock Protein 27 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunocytochemistry-Immunofluorescence-NB120-5579-img0006.jpg)
![Immunohistochemistry-Paraffin: HSP27 Antibody [NB120-5579] - Both normal and cancer biopsies of deparaffinized human Skeletal muscle tissue.](http://images.novusbio.com/fullsize/HSP27-Antibody-Immunohistochemistry-Paraffin-NB120-5579-img0008.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Western blot analysis of lysates from wild type (WT) and HMGB1/HMG-1 knockout (KO) HeLa cells using [KO Validated] HMGB1/HMG-1 Rabbit mAb at 1:6000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.5s.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517352784.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Western blot analysis of lysates from wild type (WT) and HMGB1/HMG-1 knockout (KO) HeLa cells using [KO Validated] HMGB1/HMG-1 Rabbit mAb at 1:6000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.5s.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1C4T5) [NBP3-15755] - Confocal imaging of NIH-3T3 cells using [KO Validated] HMGB1 Rabbit mAb (dilution 1:100) (Red). The cells were counterstained with alpha-Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-immunocytochemistry-immunofluorescence-1962023104824.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Rat liver using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517344263.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517311012.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517304984.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517311085.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Mouse colon using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-5220251734575.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody (1C4T5) [HMGB1/HMG-1] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using [KO Validated] HMGB1/HMG-1 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-52202517352740.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (1C4T5) [NBP3-15755] - Western blot analysis of various lysates using [KO Validated] HMGB1/HMG-1 Rabbit mAb at 1:6000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 0.5s.](http://images.novusbio.com/fullsize/nbp3-15755_rabbit-hmgb1-hmg-1-mab-1c4t5-8220252010416.jpg)
![Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Western-Blot-NBP2-54789-img0005.jpg "Western Blot: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Lane 1: Molecular Weight Ladder (MW). Lane 2: Active Alpha Synuclein Protein Aggregate.")
![Immunocytochemistry/Immunofluorescence: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Aggregate at 4 ug/ml (D-F), but not when treated with control Alpha Synuclein Protein Aggregate at 4 ug/ml (A-C). Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body incluscions. Magnification: 20x.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunocytochemistry-Immunofluorescence-NBP2-54789-img0004.jpg)
![Immunohistochemistry: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Immunohistochemistry analysis of rat brain injected with Type 1 human alpha synuclein PFFs (NBP2-54789). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 human alpha synuclein PFFs (NBP2-54789) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30-days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the striatum close to an injection site.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-54789-img0007.jpg)
![In vitro assay: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate (NBP2-54789) is combined with 100 uM of active alpha Synuclein monomer (NBP2-54788), as compared to active alpha Synuclein aggregate (NBP2-54789) and active alpha Synuclein monomer (NBP2-54788) alone. Thioflavin T ex = 450 nm, em = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-54789-img0006.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0008.jpg)
![Product Image: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - Toxicity results comparing Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 2) and Active Human Recombinant alpha-Synuclein Pre-formed Fibrils (Type 1) (Catalog No. NBP2-54789). Data was graphed after live cell imaging results were obtained using the following procedure: After 8 days in vitro, primary rat mixed cortical neuron cells were washed with 1X PBS and treated with 500 g/ml of Type 1 and Type 2 alpha-Synuclein Proteins for 20 hours at 37C. Following treatements, cells were washed with 2X PBS and incubated with a staining solution (2.0 M Cell Event + 2.5 M Ethidium homodimer + 2.5 g/ml Hoechst 33342 in sterile HBSS) for 30 minutes at 37C. The addition of the Type 2 alpha-Synuclein Proteins resulted in a significant increase in cell death.](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Product-Image-NBP2-54789-img0010.jpg)
![Electron Microscopy: Recombinant Human alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-54789] - TEM of Type 1 alpha-Synuclein Pre-formed Fibrils (PFFs) (NBP2-54789)](http://images.novusbio.com/fullsize/Recombinant-Human-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-54789-img0009.jpg)
![Western Blot: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2,000 dilution.Positive control:Lane 1: 293 Lane 2: MCF-7Lane 3: SK-Br-3 Lane 4: Mouse kidney](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Western-Blot-NBP2-75440-img0009.jpg "Western Blot: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2,000 dilution.Positive control:Lane 1: 293 Lane 2: MCF-7Lane 3: SK-Br-3 Lane 4: Mouse kidney")
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Staining STUB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunocytochemistry-Immunofluorescence-NBP2-75440-img0004.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded rat epididymis tissue using anti-STUB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunohistochemistry-Paraffin-NBP2-75440-img0008.jpg)
![Flow Cytometry: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of SH-SY-5Y cells with STUB1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Flow-Cytometry-NBP2-75440-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Staining STUB1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunocytochemistry-Immunofluorescence-NBP2-75440-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Staining STUB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunocytochemistry-Immunofluorescence-NBP2-75440-img0003.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded human colon cancer tissue using anti-STUB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunohistochemistry-Paraffin-NBP2-75440-img0005.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded human placenta tissue using anti-STUB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunohistochemistry-Paraffin-NBP2-75440-img0006.jpg)
![Immunohistochemistry-Paraffin: CHIP/STUB1 Antibody (JG38-22) [NBP2-75440] - Analysis of paraffin-embedded mouse kidney tissue using anti-STUB1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-JG38-22-Immunohistochemistry-Paraffin-NBP2-75440-img0007.jpg)
![Western Blot: Mucolipin 1 Antibody [NB110-82375] - Total protein from mouse brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Mucolipin 1 (NB110-82375) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Western-Blot-NB110-82375-img0005.jpg "Western Blot: Mucolipin 1 Antibody [NB110-82375] - Total protein from mouse brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Mucolipin 1 (NB110-82375) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).")
![Simple Western: Mucolipin 1 Antibody [NB110-82375] - Lane view shows a specific band for Mucolipin 1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Mucolipin-1-Antibody-Simple-Western-NB110-82375-img0004.jpg)
![Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Knockout-Validated-NBP2-25149-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of tissue or whole cell lysates using mouse mAb to HSP27, NBP2-25149, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong single band at ~27 kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific and unreactive with rodent HSP27. The blot was simultaneously probed with rabbit pAb to HSP-60, dilution 1:5,000 in red. A strong 60 kDa band is present in all preparations.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Western-Blot-NBP2-25149-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of HeLa cells stained with mouse HSP27 mAb, dilution 1:2000 (Green), and costained with chicken HSP60 pAb, dilution 1:2000 (Red). DAPI staining of nuclear DNA (Blue). The HSP27 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0008.jpg)
![Flow Cytometry: Hsp27 Antibody (6H11) [NBP2-25149] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Flow-Cytometry-NBP2-25149-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp27 Antibody (6H11) [NBP2-25149] - HeLa cells stained with NBP2-25149 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25149 reveals strong cytoplasmic staining and penetrates into the Actin rich ruffled margins, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0003.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Analysis of lysates from different cell lines probed with mouse mAb to high mobility group protein B1, (HMGB1), NBP2-25148, dilution 1:2,000: [1] protein standard, [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, and [6] SH-SY5Y. The 25kDa band revealed by MCA-1F3 antibody, corresponds to HMGB1 protein.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1F3-Western-Blot-NBP2-25148-img0009.jpg "Western Blot: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Analysis of lysates from different cell lines probed with mouse mAb to high mobility group protein B1, (HMGB1), NBP2-25148, dilution 1:2,000: [1] protein standard, [2] NIH-3T3, [3] C6, [4] HEK293, [5] HeLa, and [6] SH-SY5Y. The 25kDa band revealed by MCA-1F3 antibody, corresponds to HMGB1 protein.")
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Analysis of of HeLa cells stained with mouse mAb to HMGB1,NBP2-25148, dilution 1:2,000 in red and costained with rabbit pAb to GAPDH, dilution 1:2,000 in green. MCA-1F3 antibody stains the chromatin binding protein HMGB1, which is localized in the nuclei.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP2-25148-img0010.jpg)
![Flow Cytometry: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using HMGB1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1F3-Flow-Cytometry-NBP2-25148-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - HeLa cells grown in tissue culture and stained with NBP2-25148 (red), chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25148 reveals strong nuclear staining which overlaps with the DNA stain.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP2-25148-img0008.jpg)
![Simple Western: HMGB1/HMG-1 Antibody (1F3) [NBP2-25148] - Simple Western lane view shows a specific band for HMGB1 in 0.05 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1F3-Simple-Western-NBP2-25148-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Calpain 2 Antibody [NBP1-88204] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88204-img0007.jpg "Immunocytochemistry/Immunofluorescence: Calpain 2 Antibody [NBP1-88204] - Staining of human cell line U-251 MG shows localization to cytosol. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Analysis in human lung and liver tissues. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0008.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0014.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0011.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human cervix, uterine shows weak to moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0012.jpg)
![Immunohistochemistry-Paraffin: Calpain 2 Antibody [NBP1-88204] - Staining of human kidney shows moderate cytoplasmic positivity in cells in glomeruli and in distal tubules.](http://images.novusbio.com/fullsize/Calpain-2-Antibody-Immunohistochemistry-Paraffin-NBP1-88204-img0013.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/nbp1-88204_rabbit-polyclonal-calpain-2-antibody-652025162443.jpg)
![Western Blot: PSMA1 Antibody [NBP1-81768] - Analysis using Anti-PSMA1 antibody NBP1-81768 (A) shows similar pattern to independent antibody NBP2-37908 (B).](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Western-Blot-NBP1-81768-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PSMA1 Antibody [NBP1-81768] - Analysis using Anti-PSMA1 antibody NBP1-81768 (A) shows similar pattern to independent antibody NBP2-37908 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PSMA1 Antibody [NBP1-81768] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81768-img0007.jpg)
![Immunohistochemistry-Paraffin: PSMA1 Antibody [NBP1-81768] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-81768-img0011.jpg)
![Western Blot: PSMA1 Antibody [NBP1-81768] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Western-Blot-NBP1-81768-img0006.jpg)
![Immunohistochemistry-Paraffin: PSMA1 Antibody [NBP1-81768] - Staining of human testis shows distinct cytoplasmic and nuclear positivity in cells in seminiferus ducts and Leydig cells.](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-81768-img0008.jpg)
![Immunohistochemistry-Paraffin: PSMA1 Antibody [NBP1-81768] - Staining of human cervix, uterine shows moderate to strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-81768-img0010.jpg)
![Immunohistochemistry-Paraffin: PSMA1 Antibody [NBP1-81768] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/Proteasome-20S-C2-Antibody-Immunohistochemistry-Paraffin-NBP1-81768-img0012.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining in human epididymis and pancreas tissues using anti-GSS antibody. Corresponding GSS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0006.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining in human epididymis and pancreas tissues using anti-GSS antibody. Corresponding GSS RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human cerebral cortex, colon, epididymis and testis using Anti-GSS antibody NBP2-57177 (A) shows similar protein distribution across tissues to independent antibody NBP2-30465 (B).](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Glutathione Synthetase Antibody [NBP2-57177] - Analysis using Anti-GSS antibody NBP2-57177 (A) shows similar pattern to independent antibody NBP2-30465 (B).](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Western-Blot-NBP2-57177-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human cell line U-2 OS shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57177-img0007.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human testis.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0012.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0005.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human colon.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0010.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0011.jpg)
![Immunohistochemistry-Paraffin: Glutathione Synthetase Antibody [NBP2-57177] - Staining of human epididymis using Anti-GSS antibody NBP2-57177.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP2-57177-img0013.jpg)