| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38661-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49153-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21188-25ul | |||
| Azide and BSA Free | NBP3-44147 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15136-25ul | |||
| Azide and BSA Free | NBP3-44185 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77370-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61828-0.025ml | |||
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta-III Tubulin antibody [GT1338] detects beta-III Tubulin protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta-III Tubulin protein stained by beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:500. Red: NeuN, stained by NeuN antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Immunocytochemistry-Immunofluorescence-NBP3-13505-img0002.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (NBP3-13505) diluted by 1:1000.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0001.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0003.jpg)
![beta Tubulin 3/ Tuj1 antibody [GT1338] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13505_mouse-beta-iii-tubulin-mab-gt1338-immunohistochemistry-paraffin-2611202518393116.jpg)
![Western Blot: Synapsin II Antibody [NBP3-13445] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody (NBP3-13445) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Synapsin-II-Antibody-Western-Blot-NBP3-13445-img0002.jpg "Western Blot: Synapsin II Antibody [NBP3-13445] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody (NBP3-13445) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Synapsin II Antibody [NBP3-13445] - Rat brain. Synapsin II stained by Synapsin II antibody (NBP3-13445) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Synapsin-II-Antibody-Immunohistochemistry-Paraffin-NBP3-13445-img0005.jpg)
![Western Blot: Synapsin II Antibody [NBP3-13445] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Synapsin II antibody (NBP3-13445) diluted at 1:500000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Synapsin-II-Antibody-Western-Blot-NBP3-13445-img0001.jpg)
![Immunohistochemistry-Paraffin: Synapsin II Antibody [NBP3-13445] - Mouse brain. Synapsin II stained by Synapsin II antibody (NBP3-13445) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Synapsin-II-Antibody-Immunohistochemistry-Paraffin-NBP3-13445-img0004.jpg)
![Synapsin II antibody detects Synapsin II protein at Synapse by immunofluorescent analysis.Sample: DIV10 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Synapsin II stained by Synapsin II antibody (NBP3-13445) diluted at 1:500.Red: Tau, a marker, stained by Tau antibody [GT287] diluted at 1:500.](http://images.novusbio.com/fullsize/nbp3-13445_rabbit-synapsin-ii-pab-immunocytochemistry-immunofluorescence-2611202518283516.jpg)
![Immunohistochemistry-Paraffin: DAB1 Antibody [NBP2-38661] - Analysis in human small intestine and placenta tissues using NBP2-38661 antibody. Corresponding DAB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DAB1-Antibody-Immunohistochemistry-Paraffin-NBP2-38661-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: DAB1 Antibody [NBP2-38661] - Analysis in human small intestine and placenta tissues using NBP2-38661 antibody. Corresponding DAB1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: DAB1 Antibody [NBP2-38661] - Staining of human cell line RT4 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DAB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38661-img0002.jpg)
![Immunohistochemistry-Paraffin: DAB1 Antibody [NBP2-38661] - Staining of human small intestine shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/DAB1-Antibody-Immunohistochemistry-Paraffin-NBP2-38661-img0007.jpg)
![Immunohistochemistry-Paraffin: DAB1 Antibody [NBP2-38661] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/DAB1-Antibody-Immunohistochemistry-Paraffin-NBP2-38661-img0008.jpg)
![Immunohistochemistry-Paraffin: DAB1 Antibody [NBP2-38661] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/DAB1-Antibody-Immunohistochemistry-Paraffin-NBP2-38661-img0009.jpg)
![Immunohistochemistry-Paraffin: DAB1 Antibody [NBP2-38661] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/DAB1-Antibody-Immunohistochemistry-Paraffin-NBP2-38661-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: PTPRD Antibody [NBP2-49153] - Staining of human cell line RH-30 shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PTPRD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-49153-img0003.jpg "Immunocytochemistry/Immunofluorescence: PTPRD Antibody [NBP2-49153] - Staining of human cell line RH-30 shows localization to vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: PTPRD Antibody [NBP2-49153] - Staining of mouse brain shows moderate cytoplasmic positivity in mitral cell layer in olfactory bulb.](http://images.novusbio.com/fullsize/PTPRD-Antibody-Immunohistochemistry-Paraffin-NBP2-49153-img0012.jpg)
![Immunohistochemistry-Paraffin: PTPRD Antibody [NBP2-49153] - Staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells in medulla.](http://images.novusbio.com/fullsize/PTPRD-Antibody-Immunohistochemistry-Paraffin-NBP2-49153-img0008.jpg)
![Immunohistochemistry-Paraffin: PTPRD Antibody [NBP2-49153] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/PTPRD-Antibody-Immunohistochemistry-Paraffin-NBP2-49153-img0009.jpg)
![Immunohistochemistry-Paraffin: PTPRD Antibody [NBP2-49153] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/PTPRD-Antibody-Immunohistochemistry-Paraffin-NBP2-49153-img0010.jpg)
![Immunohistochemistry-Paraffin: PTPRD Antibody [NBP2-49153] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/PTPRD-Antibody-Immunohistochemistry-Paraffin-NBP2-49153-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: OTP Antibody (CL11222) [NBP3-15136] - Staining of MCF7 cells using the Anti-OTP monoclonal antibody, showing specific staining in nuclear bodies and in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively.](http://images.novusbio.com/fullsize/OTP-Antibody-CL11222-Immunocytochemistry-Immunofluorescence-NBP3-15136-img0009.jpg "Immunocytochemistry/Immunofluorescence: OTP Antibody (CL11222) [NBP3-15136] - Staining of MCF7 cells using the Anti-OTP monoclonal antibody, showing specific staining in nuclear bodies and in the nucleoplasm in green. Microtubule and nuclear probes are visualized in red and blue, respectively.")
![Immunohistochemistry-Paraffin: OTP Antibody (CL11222) [NBP3-15136] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/OTP-Antibody-CL11222-Immunohistochemistry-Paraffin-NBP3-15136-img0008.jpg)
![Immunohistochemistry-Paraffin: OTP Antibody (CL11222) [NBP3-15136] - Staining of human hypothalamus shows moderate to strong nuclear positivity in a subset of neurons.](http://images.novusbio.com/fullsize/OTP-Antibody-CL11222-Immunohistochemistry-Paraffin-NBP3-15136-img0003.jpg)
![Immunohistochemistry-Paraffin: OTP Antibody (CL11222) [NBP3-15136] - Staining of human lung tumor (typical carcinoid) shows strong nuclear positivity in tumor cells.](http://images.novusbio.com/fullsize/OTP-Antibody-CL11222-Immunohistochemistry-Paraffin-NBP3-15136-img0005.jpg)
![Immunohistochemistry-Paraffin: OTP Antibody (CL11222) [NBP3-15136] - Staining of rat hypothalamus shows strong nuclear positivity in a subset of neurons.](http://images.novusbio.com/fullsize/OTP-Antibody-CL11222-Immunohistochemistry-Paraffin-NBP3-15136-img0007.jpg)
![Immunohistochemistry-Paraffin: OTP Antibody (CL11222) [NBP3-15136] - Staining of human lymph node shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/OTP-Antibody-CL11222-Immunohistochemistry-Paraffin-NBP3-15136-img0006.jpg)
![Western Blot: SRPX2 Antibody [NBP1-77370] - Human lung tissue lysate with SRPX2 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/SRPX2-Antibody-Western-Blot-NBP1-77370-img0001.jpg "Western Blot: SRPX2 Antibody [NBP1-77370] - Human lung tissue lysate with SRPX2 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry: SRPX2 Antibody - BSA Free [NBP1-77370] - Immunohistochemistry of SRPX2 in rat lung tissue with SRPX2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77370_rabbit-polyclonal-srpx2-antibody-271120241945120.jpg)
![Immunocytochemistry/ Immunofluorescence: SRPX2 Antibody - BSA Free [NBP1-77370] - Immunofluorescence of SRPX2 in human lung tissue with SRPX2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77370_rabbit-polyclonal-srpx2-antibody-2711202419261848.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846] - Staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-68846-img0006.jpg "Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846] - Staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Glutamate Dehydrogenase Antibody [NBP1-68846] - (3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Immunohistochemistry-Paraffin-NBP1-68846-img0002.jpg)
![Western Blot: Glutamate Dehydrogenase Antibody [NBP1-68846] - Staining of Human (A) and Mouse (B) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glutamate-Dehydrogenase-Antibody-Western-Blot-NBP1-68846-img0004.jpg)
![Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Western blot analysis of extracts of various cell lines, using Muscarinic AChR M2 (Muscarinic Acetylcholine Receptor M2/CHRM2) Rabbit mAb (NBP3-16576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-3T5W8-Western-Blot-NBP3-16576-img0004.jpg "Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Western blot analysis of extracts of various cell lines, using Muscarinic AChR M2 (Muscarinic Acetylcholine Receptor M2/CHRM2) Rabbit mAb (NBP3-16576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Analysis of paraffin-embedded Human esophageal cancer using Muscarinic AChR M2 (Muscarinic AChR M2 (ACM2)) Rabbit mAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16576_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-3t5w8-immunohistochemistry-paraffin-3152024111317..jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Human tonsil tissue using Muscarinic AChR M2 (ACM2) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16576_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-3t5w8-immunohistochemistry-paraffin-3152024111413..jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Mouse kidney tissue using Muscarinic AChR M2 (ACM2) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16576_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-3t5w8-immunohistochemistry-paraffin-315202411152..jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Mouse testis tissue using Muscarinic AChR M2 (ACM2) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16576_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-3t5w8-immunohistochemistry-paraffin-315202411165..jpg)
![Immunohistochemistry: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Muscarinic AChR M2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16576_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-3t5w8-52202511402786.jpg)
![Western Blot: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis using GRM5 mAb against human GRM5 (AA: extra 458-580) recombinant protein. (Expected MW is 40.1 kDa)](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-Western-Blot-NBP2-61828-img0005.jpg "Western Blot: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis using GRM5 mAb against human GRM5 (AA: extra 458-580) recombinant protein. (Expected MW is 40.1 kDa)")
![Immunocytochemistry/Immunofluorescence: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis of Hela cells using GRM5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-Immunocytochemistry-Immunofluorescence-NBP2-61828-img0002.jpg)
![Immunohistochemistry: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis of paraffin-embedded stomach cancer tissues using GRM5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-Immunohistochemistry-NBP2-61828-img0004.jpg)
![Western Blot: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis using GRM5 mAb against HEK293 (1) and GRM5 (AA: extra 458-580)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-Western-Blot-NBP2-61828-img0006.jpg)
![Immunohistochemistry: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis of paraffin-embedded bladder cancer tissues using GRM5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-Immunohistochemistry-NBP2-61828-img0003.jpg)
![ELISA: mGluR5 Antibody (1A11D10) [NBP2-61828] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-ELISA-NBP2-61828-img0001.jpg)
![Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (JE38-35) [NBP3-32617] - Western blot analysis of Muscarinic Acetylcholine Receptor M2/CHRM2 on different lysates with Rabbit anti-Muscarinic Acetylcholine Receptor M2/CHRM2 antibody (NBP3-32617) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: U-87 MG cell lysate Lane 4: Neuro-2a cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32617) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32617_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-27820241631631.jpg "Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (JE38-35) [NBP3-32617] - Western blot analysis of Muscarinic Acetylcholine Receptor M2/CHRM2 on different lysates with Rabbit anti-Muscarinic Acetylcholine Receptor M2/CHRM2 antibody (NBP3-32617) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: U-87 MG cell lysate Lane 4: Neuro-2a cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32617) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (JE38-35) [NBP3-32617] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Muscarinic Acetylcholine Receptor M2/CHRM2 antibody (NBP3-32617) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32617) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32617_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-29820241435280.jpg)
![Immunocytochemistry/ Immunofluorescence: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (JE38-35) [NBP3-32617] - Immunocytochemistry analysis of SH-SY5Y cells labeling Muscarinic Acetylcholine Receptor M2/CHRM2 with Rabbit anti-Muscarinic Acetylcholine Receptor M2/CHRM2 antibody (NBP3-32617) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Muscarinic Acetylcholine Receptor M2/CHRM2 antibody (NBP3-32617) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃.Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32617_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-29202410484825.jpg)
![Flow Cytometry: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (JE38-35) [NBP3-32617] - Flow cytometric analysis of SH-SY5Y cells labeling Muscarinic Acetylcholine Receptor M2/CHRM2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32617, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32617_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-392024884617.jpg)