| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87885-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80769-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13780-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93530-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92928-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50444-0.025ml | |||
![Immunocytochemistry/Immunofluorescence: LEDGF Antibody [NBP1-87885] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87885-img0008.jpg "Immunocytochemistry/Immunofluorescence: LEDGF Antibody [NBP1-87885] - Staining of human cell line U-2 OS shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Semaphorin 4C Antibody [NBP1-80769] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/Semaphorin-4C-Antibody-Immunohistochemistry-Paraffin-NBP1-80769-img0007.jpg "Immunohistochemistry-Paraffin: Semaphorin 4C Antibody [NBP1-80769] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.")
![Immunohistochemistry-Paraffin: Semaphorin 4C Antibody [NBP1-80769] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Semaphorin-4C-Antibody-Immunohistochemistry-Paraffin-NBP1-80769-img0004.jpg)
![Immunohistochemistry-Paraffin: Semaphorin 4C Antibody [NBP1-80769] - Staining of human duodenum shows moderate positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/Semaphorin-4C-Antibody-Immunohistochemistry-Paraffin-NBP1-80769-img0005.jpg)
![Immunohistochemistry-Paraffin: Semaphorin 4C Antibody [NBP1-80769] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Semaphorin-4C-Antibody-Immunohistochemistry-Paraffin-NBP1-80769-img0006.jpg)
![Immunohistochemistry-Paraffin: Semaphorin 4C Antibody [NBP1-80769] - Staining of human placenta shows moderate membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Semaphorin-4C-Antibody-Immunohistochemistry-Paraffin-NBP1-80769-img0008.jpg)
![Western Blot: xCT Antibody (A7C6-R) [NBP3-33104] - Western blot analysis of xCT on different lysates with Mouse anti-xCT antibody (NBP3-33104) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-288202414191525.jpg "Western Blot: xCT Antibody (A7C6-R) [NBP3-33104] - Western blot analysis of xCT on different lysates with Mouse anti-xCT antibody (NBP3-33104) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: xCT Antibody (A7C6-R) [NBP3-33104] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-xCT antibody (NBP3-33104) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33104) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-298202413585586.jpg)
![Immunocytochemistry/ Immunofluorescence: xCT Antibody (A7C6-R) [NBP3-33104] - Immunocytochemistry analysis of HT-29 cells labeling xCT with Mouse anti-xCT antibody (NBP3-33104) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-xCT antibody (NBP3-33104) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-2920241114813.jpg)
![Flow Cytometry: xCT Antibody (A7C6-R) [NBP3-33104] - Flow cytometric analysis of A549 cells labeling xCT. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-33104, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-29202416372152.jpg)
![Western Blot: SLC31A1/CTR1 Antibody [NBP3-12348] - 1:1000 antibody dilution in DiluObuffer. Labesl monomeric, dimeric and multimeric Human CTR1.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-Western-Blot-NBP3-12348-img0001.jpg "Western Blot: SLC31A1/CTR1 Antibody [NBP3-12348] - 1:1000 antibody dilution in DiluObuffer. Labesl monomeric, dimeric and multimeric Human CTR1.")
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody [NBP3-12348] - Mouse Lung. Mouse liver. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-Immunohistochemistry-Paraffin-NBP3-12348-img0005.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody [NBP3-12348] - Mouse Liver. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 5X magnification.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-Immunohistochemistry-Paraffin-NBP3-12348-img0002.jpg)
![Immunohistochemistry-Paraffin: SLC31A1/CTR1 Antibody [NBP3-12348] - Mouse liver. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification.](http://images.novusbio.com/fullsize/SLC31A1-CTR1-Antibody-Immunohistochemistry-Paraffin-NBP3-12348-img0003.jpg)
![Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1470] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48655_rabbit-melanocortin-1-r-mc1r-mab-hl1470-western-blot-2592025864.png "Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1470] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1470] (NBP3-48655) diluted at 1:50000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48655_rabbit-melanocortin-1-r-mc1r-mab-hl1470-western-blot-141120251831322.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Melanocortin-1 R/MC1R antibody [HL1470] (NBP3-48655) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48655_rabbit-melanocortin-1-r-mc1r-mab-hl1470-western-blot-141120251828111.jpg)
![<b>Independent Antibodies Validation. </b>Various tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1470] (NBP3-48655) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48655_rabbit-melanocortin-1-r-mc1r-mab-hl1470-western-blot-141120251831212.jpg)
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Non-transfected (-) and transfected (+) unboiled A375 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1470] (NBP3-48655) diluted at 1:5000 and competitor's antibody (Highly Cited Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48655_rabbit-melanocortin-1-r-mc1r-mab-hl1470-western-blot-141120251836367.jpg)
![Immunohistochemistry-Paraffin: PMCH Antibody [NBP2-13780] - Staining of human cerebral cortex, hypothalamus, liver and testis using Anti-PMCH antibody NBP2-13780 (A) shows similar protein distribution across tissues to independent antibody NBP2-57697 (B).](http://images.novusbio.com/fullsize/PMCH-Antibody-Immunohistochemistry-Paraffin-NBP2-13780-img0012.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PMCH Antibody [NBP2-13780] - Staining of human cerebral cortex, hypothalamus, liver and testis using Anti-PMCH antibody NBP2-13780 (A) shows similar protein distribution across tissues to independent antibody NBP2-57697 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PMCH Antibody [NBP2-13780] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/PMCH-Antibody-Immunohistochemistry-NBP2-13780-img0013.jpg)
![Immunohistochemistry-Paraffin: PMCH Antibody [NBP2-13780] - Staining of human hypothalamus shows strong cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/PMCH-Antibody-Immunohistochemistry-Paraffin-NBP2-13780-img0002.jpg)
![Immunohistochemistry-Paraffin: PMCH Antibody [NBP2-13780] - Staining of rat hypothalamus shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/PMCH-Antibody-Immunohistochemistry-Paraffin-NBP2-13780-img0007.jpg)
![Immunohistochemistry: PMCH Antibody [NBP2-13780] - Staining of mouse brain shows strong positivity in of neurons in the hypothalamus](http://images.novusbio.com/fullsize/PMCH-Antibody-Immunohistochemistry-Paraffin-NBP2-13780-img0009.jpg)
![Immunohistochemistry-Paraffin: PMCH Antibody [NBP2-13780] - Staining of human cerebral cortex shows no positivity in neurons.](http://images.novusbio.com/fullsize/PMCH-Antibody-Immunohistochemistry-Paraffin-NBP2-13780-img0010.jpg)
![Western Blot: MYRIP Antibody [NB100-1278] - Staining of Human Amygdala lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. (0.3ug/ml)](http://images.novusbio.com/fullsize/MYRIP-Antibody-Western-Blot-NB100-1278-img0001.jpg "Western Blot: MYRIP Antibody [NB100-1278] - Staining of Human Amygdala lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. (0.3ug/ml)")
![Immunohistochemistry-Paraffin: MYRIP Antibody [NB100-1278] - staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MYRIP-Antibody-Immunohistochemistry-Paraffin-NB100-1278-img0004.jpg)
![Immunohistochemistry-Paraffin: MYRIP Antibody [NB100-1278] - staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MYRIP-Antibody-Immunohistochemistry-Paraffin-NB100-1278-img0002.jpg)
![Immunohistochemistry-Paraffin: MYRIP Antibody [NB100-1278] - staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MYRIP-Antibody-Immunohistochemistry-Paraffin-NB100-1278-img0003.jpg)
![Western Blot: MT-ND4 Antibody [NBP2-93530] - Analysis of extracts of various cell lines, using MT-ND4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Western-Blot-NBP2-93530-img0005.jpg "Western Blot: MT-ND4 Antibody [NBP2-93530] - Analysis of extracts of various cell lines, using MT-ND4 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3min.")
![Immunocytochemistry/Immunofluorescence: MT-ND4 Antibody [NBP2-93530] - Analysis of L929 cells using MT-ND4 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/MT-ND4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93530-img0001.jpg)
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-93530] -Analysis of rat kidney using MT-ND4 Rabbit pAb at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp2-93530_rabbit-polyclonal-mt-nd4-antibody-immunohistochemistry-paraffin-247202491744..jpg)
![Immunohistochemistry-Paraffin: MT-ND4 Antibody [NBP2-93530] -Analysis of Rat ovary using MT-ND4 Rabbit pAb at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp2-93530_rabbit-polyclonal-mt-nd4-antibody-immunohistochemistry-paraffin-247202491923..jpg)
![Simple Western: ATP5A Antibody [NBP2-92928] - ATP5A Antibodies (NBP2-92928), ProteinSimple Western Blot on Jess Instrument. One microgram of human brain tissue lysate was tested with the antibodies diluted 1:10 or 1:20 times. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-92928_rabbit-polyclonal-atp5a-antibody-simple-western-2042023113534.jpg "Simple Western: ATP5A Antibody [NBP2-92928] - ATP5A Antibodies (NBP2-92928), ProteinSimple Western Blot on Jess Instrument. One microgram of human brain tissue lysate was tested with the antibodies diluted 1:10 or 1:20 times. Image from verified customer review.")
![Western Blot: ATP5A Antibody [NBP2-92928] - Analysis of extracts of various cell lines, using ATP5A at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-92928-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5A Antibody - BSA Free [NBP2-92928] - Confocal immunofluorescence analysis of Hela cells using ATP5A Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92928_rabbit-polyclonal-atp5a-antibody-4220251430450.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5A Antibody - BSA Free [NBP2-92928] - Confocal immunofluorescence analysis of U-2OS cells using ATP5A Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92928_rabbit-polyclonal-atp5a-antibody-4220251429052.jpg)
![Western Blot: LRAT Antibody (M34-P1F10) [NBP2-50444] - Shows detection of LRAT in an overexpression lysate.](http://images.novusbio.com/fullsize/LRAT-Antibody-M34-P1F10-Western-Blot-NBP2-50444-img0002.jpg "Western Blot: LRAT Antibody (M34-P1F10) [NBP2-50444] - Shows detection of LRAT in an overexpression lysate.")
![Immunocytochemistry/Immunofluorescence: LRAT Antibody (M34-P1F10) [NBP2-50444]](http://images.novusbio.com/fullsize/LRAT-Antibody-M34-P1F10-Immunocytochemistry-Immunofluorescence-NBP2-50444-img0001.jpg)
![Immunohistochemistry: LRAT Antibody (M34-P1F10) [NBP2-50444]](http://images.novusbio.com/fullsize/LRAT-Antibody-M34-P1F10-Immunohistochemistry-NBP2-50444-img0003.jpg)
![Immunohistochemistry: LRAT Antibody (M34-P1F10) [NBP2-50444] - Staining of normal, primary and metastatic colorectal tumour tissue sections using anti-LRAT [M34-P1F10]. No antigen retrieval step was required.](http://images.novusbio.com/fullsize/LRAT-Antibody-M34-P1F10-Immunohistochemistry-NBP2-50444-img0004.jpg)
![Western Blot: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319] - analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S96-55 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/GABA-A-R-beta-1-Antibody-S96-55-Western-Blot-NBP1-48319-img0004.jpg "Western Blot: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319] - analysis of Human Cell line lysates showing detection of GABA A Receptor protein using Mouse Anti-GABA A Receptor Monoclonal Antibody, Clone S96-55 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: GABA-A R beta 1 Antibody (S96-55) [NBP1-48319] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-GABA A Receptor Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Positive.](http://images.novusbio.com/fullsize/GABA-A-R-beta-1-Antibody-S96-55-Immunocytochemistry-Immunofluorescence-NBP1-48319-img0003.jpg)
![Western Blot: LEDGF Antibody (PSH02-55) [NBP3-32860] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: THP-1 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32860) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-288202412442993.jpg "Western Blot: LEDGF Antibody (PSH02-55) [NBP3-32860] - Western blot analysis of LEDGF on different lysates with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: 293T cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: THP-1 cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 60 kDa Observed band size: 75 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32860) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32860) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-298202414333682.jpg)
![Immunohistochemistry: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunofluorescence analysis of paraffin-embedded human testis tissue labeling LEDGF with Rabbit anti-LEDGF antibody (NBP3-32860) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32860, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-29820241420980.jpg)
![Immunocytochemistry/ Immunofluorescence: LEDGF Antibody (PSH02-55) [NBP3-32860] - Immunocytochemistry analysis of SH-SY5Y cells labeling LEDGF with Mouse anti-LEDGF antibody (NBP3-32860) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-LEDGF antibody (NBP3-32860) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32860_rabbit-ledgf-mab-psh02-55-29202410234271.jpg)