| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59668SS | |||
| Azide and BSA Free | NBP2-80958 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73104 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-22450-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22399-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37261-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NBP2-44311SS | |||
![Western Blot: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - Total protein from Human and Mouse Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Spectrin Beta III in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-1287A-Western-Blot-NBP2-59668-img0004.jpg "Western Blot: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - Total protein from Human and Mouse Brain was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Spectrin Beta III in blocking buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Spectrin beta 3 (1287A) at 5.0ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-1287A-Immunocytochemistry-Immunofluorescence-NBP2-59668-img0002.jpg)
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (1287A) [NBP2-59668] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of human brain using Spectrin beta III antibody at a 1:1,000 dilution. The primary antibody bound to Spectrin beta III was detected with HRP-DAB detection method and the nuclei were counterstained with hematoxylin. This Spectrin beta III antibody generated a specific cytoplasmic staining in all the cells with strongest signal in neuronal cells.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-1287A-Immunohistochemistry-Paraffin-NBP2-59668-img0005.jpg)
![Flow (Intracellular): Spectrin beta 3 Antibody (1287A) [NBP2-59668] - An intracellular stain was performed on HeLa Cells with Spectrin beta 3 (1287A) antibody NBP2-59668 (blue) and a matched isotype control MAB1050 (orange). Cells were fixed with 4% paraformaldehyde, following fixation, cells were permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by rabbit IgG APC-conjugated secondary antibody (F0111, R&D Systems).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-1287A-Flow-Intracellular-NBP2-59668-img0001.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Western-Blot-NBP1-31329-img0020.jpg "Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Mouse brain. AChE stained by AChE antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Immunohistochemistry-Paraffin-NBP1-31329-img0021.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Sample (30 ug of whole cell lysate) A: Raji 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Western-Blot-NBP1-31329-img0009.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Western-Blot-NBP1-31329-img0014.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Western-Blot-NBP1-31329-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Paraformaldehyde-fixed HeLa, using AChE antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31329-img0008.jpg)
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Mouse colon. AChE antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Immunohistochemistry-Paraffin-NBP1-31329-img0010.jpg)
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Rat fore brain. AChE antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Immunohistochemistry-Paraffin-NBP1-31329-img0011.jpg)
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Rat brain. AChE antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Acetylcholinesterase-ACHE-Antibody-Immunohistochemistry-Paraffin-NBP1-31329-img0012.jpg)
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Acetylcholinesterase/ACHE antibody detects Acetylcholinesterase/ACHE protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31329_rabbit-polyclonal-acetylcholinesterase-ache-antibody-91020242091450.jpg)
![Western Blot: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-31329_rabbit-polyclonal-acetylcholinesterase-ache-antibody-910202420104428.jpg)
![Immunohistochemistry-Paraffin: Acetylcholinesterase/ACHE Antibody [NBP1-31329] - Acetylcholinesterase/ACHE antibody detects Acetylcholinesterase/ACHE protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31329_rabbit-polyclonal-acetylcholinesterase-ache-antibody-91020242001048.jpg)
![Acetylcholinesterase/ACHE antibody detects Acetylcholinesterase/ACHE protein by immunofluorescent analysis.Sample: DIV9 rat hippocampal neuron and Glia cell cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Acetylcholinesterase/ACHE stained by Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:500.Red: Tau, an axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31329_rabbit-polyclonal-acetylcholinesterase-ache-antibody-immunocytochemistry-immunofluorescence-1411202518443420.jpg)
![Acetylcholinesterase/ACHE antibody detects Acetylcholinesterase/ACHE protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: Acetylcholinesterase/ACHE protein stained by Acetylcholinesterase/ACHE antibody (NBP1-31329) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-48812_rabbit-opsin-1-medium-wave-mab-hl3435-immunohistochemistry-frozen-14112025185548.jpg)
![Western Blot: VAChT/SLC18A3 Antibody [NB100-91348] - Rat and mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated at 4C overnight.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-Western-Blot-NB100-91348-img0032.jpg "Western Blot: VAChT/SLC18A3 Antibody [NB100-91348] - Rat and mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:2000 incubated at 4C overnight.")
![Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - Rat spinal cord. The animal was perfused at a pressure of 130 mmHg with 300 ml 4% FA before being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-Immunohistochemistry-Paraffin-NB100-91348-img0031.jpg)
![Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-Immunohistochemistry-Paraffin-NB100-91348-img0015.jpg)
![Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-Immunohistochemistry-Paraffin-NB100-91348-img0016.jpg)
![Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-Immunohistochemistry-Paraffin-NB100-91348-img0018.jpg)
![Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-Immunohistochemistry-Paraffin-NB100-91348-img0026.jpg)
![Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-Immunohistochemistry-Paraffin-NB100-91348-img0028.jpg)
![Immunohistochemistry-Paraffin: VAChT/SLC18A3 Antibody [NB100-91348] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to (Vesicular Acetylcholine Transporter) VACHT: whole serum at 1 : 200 dilution incubated overnight at 4C, developed with DAB/Ni and counterstained with hematoxylin.](http://images.novusbio.com/fullsize/VAChT-SLC18A3-Antibody-Immunohistochemistry-Paraffin-NB100-91348-img0029.jpg)
![Western Blot: VGLUT1 Antibody [NBP3-13165] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VGluT1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Western-Blot-NBP3-13165-img0013.jpg "Western Blot: VGLUT1 Antibody [NBP3-13165] - Human tissue extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VGluT1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: VGLUT1 Antibody [NBP3-13165] - VGLUT1 antibody detects VGLUT1 protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGLUT1 stained by VGLUT1 antibody (NBP3-13165) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13165-img0005.jpg)
![Immunohistochemistry-Frozen: VGLUT1 Antibody [NBP3-13165] - VGLUT1 antibody detects VGLUT1 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: VGLUT1 protein stained by VGLUT1 antibody (NBP3-13165) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Frozen-NBP3-13165-img0002.jpg)
![Western Blot: Nudel Antibody (5E11) [NBP2-01086] Analysis of extracts (35ug) from 9 different cell lines by using anti-Nudel monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Western-Blot-NBP2-01086-img0009.jpg "Western Blot: Nudel Antibody (5E11) [NBP2-01086] Analysis of extracts (35ug) from 9 different cell lines by using anti-Nudel monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Nudel Antibody (5E11) [NBP2-01086] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nudel.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunocytochemistry-Immunofluorescence-NBP2-01086-img0008.jpg)
![Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human Ovary tissue using anti-Nudel mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunohistochemistry-Paraffin-NBP2-01086-img0007.jpg)
![Flow Cytometry: Nudel Antibody (5E11) [NBP2-01086] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Nudel antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Flow-Cytometry-NBP2-01086-img0001.jpg)
![Western Blot: Nudel Antibody (5E11) [NBP2-01086] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nudel (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nudel.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Western-Blot-NBP2-01086-img0002.jpg)
![Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Nudel mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunohistochemistry-Paraffin-NBP2-01086-img0003.jpg)
![Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Nudel mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunohistochemistry-Paraffin-NBP2-01086-img0004.jpg)
![Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human Kidney tissue using anti-Nudel mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunohistochemistry-Paraffin-NBP2-01086-img0005.jpg)
![Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human liver tissue using anti-Nudel mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunohistochemistry-Paraffin-NBP2-01086-img0006.jpg)
![Western Blot: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Western blot analysis of extracts of various cell lines, using TDP-43/TARDBP antibody (NBP3-15677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Western-Blot-NBP3-15677-img0007.jpg "Western Blot: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Western blot analysis of extracts of various cell lines, using TDP-43/TARDBP antibody (NBP3-15677) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Immunohistochemistry of paraffin-embedded mouse brain using TDP-43/TARDBP Rabbit mAb (NBP3-15677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Immunohistochemistry-Paraffin-NBP3-15677-img0003.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Immunohistochemistry of paraffin-embedded rat ovary using TDP-43/TARDBP Rabbit mAb (NBP3-15677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Immunohistochemistry-Paraffin-NBP3-15677-img0001.jpg)
![Immunohistochemistry-Paraffin: TDP-43/TARDBP Antibody (4R5L7) [NBP3-15677] - Immunohistochemistry of paraffin-embedded human esophageal using TDP-43/TARDBP Rabbit mAb (NBP3-15677) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TDP-43-TARDBP-Antibody-4R5L7-Immunohistochemistry-Paraffin-NBP3-15677-img0002.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517315540.jpg)
![Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Confocal imaging of HeLa cells using TDP-43/TARDBP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517352713.jpg)
![Immunoprecipitation: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunoprecipitation of TDP-43/TARDBP from 500 ug extracts of K-562 cells was performed using 2 ug of TDP-43/TARDBP Rabbit mAb . Rabbit IgG isotype control was used to precipitate the Control IgG sample. IP samples were eluted with 1X non-reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using TDP-43/TARDBP Rabbit mAb at a dilution of 1:500.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-5220251734597.jpg)
![Chromatin Immunoprecipitation: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Chromatin immunoprecipitation analysis of extracts of K562 cells, using TDP-43/TARDBP Rabbit mAb and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517344289.jpg)
![Immunocytochemistry/ Immunofluorescence: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Confocal imaging of paraffin-embedded Mouse brain tissue using TDP-43/TARDBP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517363742.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Mouse colon using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517361227.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Human colon using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517315511.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Rat spleen using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517311072.jpg)
![Immunohistochemistry: TDP-43/TARDBP Antibody (4R5L7) [TDP-43/TARDBP] - Immunohistochemistry analysis of paraffin-embedded Rat liver using TDP-43/TARDBP Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15677_rabbit-tdp-43-tardbp-mab-4r5l7-52202517304973.jpg)
![Western Blot: HCN1 Antibody (S70) [NBP1-22450] - Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Western-Blot-NBP1-22450-img0011.jpg "Western Blot: HCN1 Antibody (S70) [NBP1-22450] - Western Blot analysis of Rat brain membrane lysate showing detection of HCN1 protein using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT. Localization: Neurons.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0014.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Human Hippocampus](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0001.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: Cerebellum. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT. Localization: Cytoplasmic staining of Purkinje cells.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0012.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0013.jpg)
![Immunohistochemistry: HCN1 Antibody (S70) [NBP1-22450] - Immunohistochemistry analysis using Mouse Anti-HCN1 Monoclonal Antibody, Clone S70 (NBP1-22450). Tissue: Frozen brain section. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN1 Monoclonal Antibody (NBP1-22450) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/HCN1-Antibody-S70-Immunohistochemistry-NBP1-22450-img0015.jpg)
![Western Blot: GluR1 Antibody (S355-1) [NBP2-22399] - Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa GluR1 protein using Mouse Anti-GluR1 Monoclonal Antibody, Clone S355-1 (NBP2-22399). Load: 10 ug. Block: 5% milk + TBST. Primary Antibody: Mouse Anti-GluR1 Monoclonal Antibody (NBP2-22399) at 1:2000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.](http://images.novusbio.com/fullsize/GluR1-Antibody-S355-1-Western-Blot-NBP2-22399-img0004.jpg "Western Blot: GluR1 Antibody (S355-1) [NBP2-22399] - Western Blot analysis of Rat Brain Membrane showing detection of ~100 kDa GluR1 protein using Mouse Anti-GluR1 Monoclonal Antibody, Clone S355-1 (NBP2-22399). Load: 10 ug. Block: 5% milk + TBST. Primary Antibody: Mouse Anti-GluR1 Monoclonal Antibody (NBP2-22399) at 1:2000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~100 kDa.")
![Immunocytochemistry/Immunofluorescence: GluR1 Antibody (S355-1) [NBP2-22399] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluA1/GluR1 Monoclonal Antibody, Clone S355-1 (NBP2-22399). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluA1/GluR1 Monoclonal Antibody (NBP2-22399) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluA1/GluR1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GluR1-Antibody-S355-1-Immunocytochemistry-Immunofluorescence-NBP2-22399-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: GluR1 Antibody (S355-1) [NBP2-22399] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluA1/GluR1 Glutamate Receptor Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction. Magnification: 60X.](http://images.novusbio.com/fullsize/GluR1-Antibody-S355-1-Immunocytochemistry-Immunofluorescence-NBP2-22399-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: GluR1 Antibody (S355-1) [NBP2-22399] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluA1/GluR1 Glutamate Receptor Monoclonal Antibody, Clone S355-1 (NBP2-22399). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluA1/GluR1 Glutamate Receptor Monoclonal Antibody (NBP2-22399) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GluA1/GluR1 Glutamate Receptor Antibody. (D) Composite.](http://images.novusbio.com/fullsize/GluR1-Antibody-S355-1-Immunocytochemistry-Immunofluorescence-NBP2-22399-img0006.jpg)
![Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Western-Blot-NBP2-37261-img0004.jpg "Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mouse mAb against Hela (1), SK-N-SH (2), HepG2 (3), C6 (4), PC-12 (5), PANC-1 (6), NIH/3T3 (7) cell lysate.")
![Immunocytochemistry/Immunofluorescence: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis of MCF-7 cells using BACE1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Immunocytochemistry-Immunofluorescence-NBP2-37261-img0005.jpg)
![Flow Cytometry: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis of Hela cells using BACE1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Flow-Cytometry-NBP2-37261-img0006.jpg)
![Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mAb against human BACE1 (AA: 112-324) recombinant protein. (Expected MW is 49.9 kDa)](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Western-Blot-NBP2-37261-img0002.jpg)
![Western Blot: BACE-1 Antibody (3C1C3) [NBP2-37261] - Analysis using BACE1 mAb against HEK293 (1) and BACE1 (AA: 112-324)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-Western-Blot-NBP2-37261-img0003.jpg)
![ELISA: BACE-1 Antibody (3C1C3) [NBP2-37261] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/BACE-1-Antibody-3C1C3-ELISA-NBP2-37261-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Spectrin beta 3 (3B1NB) conjugated to Alexa Fluor 488 [NBP2-44310AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-3B1NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-44311-img0005.jpg "Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-Spectrin beta 3 (3B1NB) conjugated to Alexa Fluor 488 [NBP2-44310AF488] at 10ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - IHC analysis of a formalin fixed and paraffin embedded tissue section of normal human prostate using 1:200 dilution of Spectrin beta 3 antibody (clone 3B1NB). The antibody generated a specific cytoplasmic staining with strong signal at cell-cell junctions in the glandular epithelium of the prostate section. The cells of fibromuscular stroma showed a diffused signal throughout the sections. Image using the standard format of this product.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-3B1NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-44311-img0006.jpg)
![Flow (Cell Surface): Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - Spectrin B3 antibody (clone 3B1NB) was used to stain HeLa cells (1 x 10^6 cells/ml) at a 1:500 dilution detected using Dylight-488 conjugated goat anti-rat IgG secondary antibody (blue peak). Shown with secondary control (orange peak).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-3B1NB-Azide-Free-Flow-Cell-Surface-NBP2-44311-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody (3B1NB) - Azide Free [NBP2-44311] - A431 cells were fixed for 10 minutes using -20C MeOH. The cells were incubated with anti-Spectrin beta 3 (3B1NB) [NBP2-44311-azide free] at a 1:50 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-3B1NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-44311-img0004.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0021.jpg "Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1ug/mL overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.")
![Immunocytochemistry/Immunofluorescence: beta Amyloid Antibody [NBP1-78007]](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78007-img0015.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Human Heart (formalin-fixed, paraffin-embedded) stained with beta Amyloid antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0024.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Mouse Brain (Lane 1) and Mouse Spinal Chord (Lane 2) were run on a 4-20% gradient gel, Blocked in 1% BSA-TBS-T 30 min RT and probed with Rb-a-Beta Amyloid 1:1000 in 1% BSA-TBS-T o/n 4C. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0010.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Lane 1: HEK293 WCL. Lane 2: Mouse Brain WCL. Lane 3: A-172 WCL. Load: 10.0ug per lane. Primary antibody: Beta Amyloid Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Conjugated Goat Anti Rabbit IgG at 1:40,000 for 30 min at RT. Block for 30 min at RT. Note: observed band higher than predicted molecular weight of 5 kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0018.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate. Lane 2: HeLa Lysate.Lane 3: MCF-7 Lysate.Lane 4: Jurkat Lysate.Lane 5: A431 Lysate.Lane 6: LNCaP Lysate.Lane 7: A-172 Lysate.Lane 8: NIH/3T3 Lysate. Load: 35 ug per lane.Primary antibody: beta Amyloid antibody at 1:5,000 for overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:30,000 for 60 min at RT.Blocking Buffer: 1% Casein-TTBS for 30 min at RT.Predicted MW: ~40-50 kDa. Observed size: ~48 kDa for beta Amyloid.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0019.jpg)
![Western Blot: beta Amyloid Antibody [NBP1-78007] - Western Blot of beta Amyloid antibody. Lane 1: Opal Prestained Molecular Weight Martker. Lane 2: HEK293T Whole Cell Lysate. Lane 3: Mouse Brain Whole Cell Lysate. Lane 4: A-172 Whole Cell Lysate.Lane 5: Daudi Whole Cell Lysate. Load: 10ug/lane. Primary Antibody: Anti-beta Amyloid at 1:1000 overnight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP Conjugated at 1:70,000 for 30min at RT. Block: BlockOut Buffer. Predicted MW: ~40-50kDa. Observed MW: ~48kDa.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Western-Blot-NBP1-78007-img0020.jpg)
![Immunohistochemistry-Paraffin: beta Amyloid Antibody [NBP1-78007] - Human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR-1 staining of human ovarian carcinoma.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-Paraffin-NBP1-78007-img0008.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistical detection of beta Amyloid using beta Amyloid antibody on TG APP23 mouse brain cortex frozen sections. beta Amyloid antibody used at 1/200 and incubated for 2 hours in TBS/BSA/Tween/azide. Fluorescent labelled anti rabbit IgG was then added. Carl Hobbs, King`s College London, United Kingdom](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0022.jpg)
![Immunohistochemistry: beta Amyloid Antibody [NBP1-78007] - Immunohistochemistry with beta Amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C). Staining was performed on Leica Bond system using the standard protocol. Formalin fixed/paraffin embedded tissue sections were subjected to antigen retrieval with E1 (Leica Microsystems) retrieval solution for 20 min and then incubated with rabbit beta Amyloid antibody at 1:100 dilution for 60 minutes. Biotinylated Anti-rabbit secondary antibody was used at 1:200 dilution to detect primary antibody. The reaction was developed using streptavidin-HRP conjugated compact polymer system and visualized with chromogen substrate, 3’3-diamino-benzidine substrate (DAB). The sections were then counterstained with hematoxylin to detect cell nuclei.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-Immunohistochemistry-NBP1-78007-img0023.jpg)
![ELISA: beta Amyloid Antibody [NBP1-78007] - ELISA results of purified beta Amyloid antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1ug of conjugate. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) and TMB ELISA Peroxidase Substrate.](http://images.novusbio.com/fullsize/beta-Amyloid-Antibody-ELISA-NBP1-78007-img0025.jpg)
