| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72828 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83349-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90815-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85802-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-19344SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-126SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73104 | |||
![Western Blot: Musashi 1 Antibody (1G8) [NBP2-03434] Analysis of extracts (35ug) from 9 different cell lines by using anti-Musashi 1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Musashi-1-Antibody-1G8-Western-Blot-NBP2-03434-img0008.jpg "Western Blot: Musashi 1 Antibody (1G8) [NBP2-03434] Analysis of extracts (35ug) from 9 different cell lines by using anti-Musashi 1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunocytochemistry/Immunofluorescence: Musashi 1 Antibody (1G8) [NBP2-03434] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Musashi 1.](http://images.novusbio.com/fullsize/Musashi-1-Antibody-1G8-Immunocytochemistry-Immunofluorescence-NBP2-03434-img0007.jpg)
![Immunohistochemistry-Paraffin: Musashi 1 Antibody (1G8) [NBP2-03434] - Staining of paraffin-embedded Human pancreas tissue using anti-Musashi 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Musashi-1-Antibody-1G8-Immunohistochemistry-Paraffin-NBP2-03434-img0006.jpg)
![Flow Cytometry: Musashi 1 Antibody (1G8) [NBP2-03434] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Musashi 1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Musashi-1-Antibody-1G8-Flow-Cytometry-NBP2-03434-img0002.jpg)
![Western Blot: Musashi 1 Antibody (1G8) [NBP2-03434] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Musashi 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Musashi 1.](http://images.novusbio.com/fullsize/Musashi-1-Antibody-1G8-Western-Blot-NBP2-03434-img0003.jpg)
![Immunohistochemistry-Paraffin: Musashi 1 Antibody (1G8) [NBP2-03434] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Musashi 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Musashi-1-Antibody-1G8-Immunohistochemistry-Paraffin-NBP2-03434-img0004.jpg)
![Immunohistochemistry-Paraffin: Musashi 1 Antibody (1G8) [NBP2-03434] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Musashi 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Musashi-1-Antibody-1G8-Immunohistochemistry-Paraffin-NBP2-03434-img0005.jpg)
![Flow Cytometry: Musashi 1 Antibody (1G8) [NBP2-03434] - Analysis of Hela cells, using anti-Musashi 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Musashi-1-Antibody-1G8-Flow-Cytometry-NBP2-03434-img0001.jpg)
![Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Profilin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Profilin 1.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Western-Blot-NBP2-73605-img0004.jpg "Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Profilin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Profilin 1.")
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Profilin 1.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73605-img0008.jpg)
![Immunohistochemistry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Staining of paraffin-embedded Human Kidney tissue using anti-Profilin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73605-img0009.jpg)
![Flow Cytometry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of Jurkat cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73605-img0007.jpg)
![Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of extracts (10ug) from 10 Human tissue by using anti-PFN1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Western-Blot-NBP2-73605-img0001.jpg)
![Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PFN1 monoclonal antibody.(1:200)](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Western-Blot-NBP2-73605-img0002.jpg)
![Western Blot: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Profilin 1 monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Western-Blot-NBP2-73605-img0003.jpg)
![Immunohistochemistry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Staining of paraffin-embedded Human pancreas tissue using anti-Profilin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73605-img0010.jpg)
![Flow Cytometry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - Analysis of Hela cells, using anti-Profilin 1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73605-img0005.jpg)
![Flow Cytometry: Profilin 1 Antibody (OTI1D5) - Azide and BSA Free [NBP2-73605] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Profilin 1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-OTI1D5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73605-img0006.jpg)
![Western Blot: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of Profilin 1 on Hela cell using anti-Profilin 1 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Western-Blot-NBP2-67078-img0008.jpg "Western Blot: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of Profilin 1 on Hela cell using anti-Profilin 1 antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Staining Profilin 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunocytochemistry-Immunofluorescence-NBP2-67078-img0004.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of paraffin-embedded mouse kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunohistochemistry-Paraffin-NBP2-67078-img0007.jpg)
![Flow Cytometry: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of Hela cells with Profilin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Flow-Cytometry-NBP2-67078-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Staining Profilin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunocytochemistry-Immunofluorescence-NBP2-67078-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Staining Profilin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunocytochemistry-Immunofluorescence-NBP2-67078-img0003.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of paraffin-embedded human kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunohistochemistry-Paraffin-NBP2-67078-img0005.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (JA30-13) [NBP2-67078] - Analysis of paraffin-embedded mouse colon tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-JA30-13-Immunohistochemistry-Paraffin-NBP2-67078-img0006.jpg)
![Western Blot: BIRC6 Antibody [NB110-40730] - Dual IAP-targeting ASOs knockdown BIRC6, cIAP1 or survivin proteins and lead to marked suppression of CRPC cell proliferation. Western blotting showing protein levels of BIRC6, cIAP1 and survivin in two CRPC cell lines (A) PC-3 cells and (B) C4-2 cells transfected with Mock or increasing dosages of scrambled ASO (Scrb), dASOs 6w2 and 6w5 for 72 hr. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/2229), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Western-Blot-NB110-40730-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: BIRC6 Antibody [NB110-40730] - Dual IAP-targeting ASOs knockdown BIRC6, cIAP1 or survivin proteins and lead to marked suppression of CRPC cell proliferation. Western blotting showing protein levels of BIRC6, cIAP1 and survivin in two CRPC cell lines (A) PC-3 cells and (B) C4-2 cells transfected with Mock or increasing dosages of scrambled ASO (Scrb), dASOs 6w2 and 6w5 for 72 hr. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/2229), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: BIRC6 Antibody [NB110-40730] - Treatments with dASOs resulted in significant lower viable tumor volume. Representative images of control ASO-6w2- and 6w5-treated PC-3 xenografts using H&E, and IHC staining of BIRC6, cleaved-caspase 3 and Ki-67. 20x magnification. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/2229), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-NB110-40730-img0005.jpg)
![Immunohistochemistry-Paraffin: BIRC6 Antibody [NB110-40730] - FFPE section of human metastatic lymph node. Antibody: Affinity purified rabbitanti-BIRC6/Apollon used at a dilution of 1:250. Detection: DAB](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-Paraffin-NB110-40730-img0003.jpg)
![Immunohistochemistry-Paraffin: BIRC6 Antibody [NB110-40730] - FFPE section of human ovarian tumor. Antibody: Affinity purified rabbit antiBIRC6/Apollon used at a dilution of 1:250. Detection: DAB](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-Paraffin-NB110-40730-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: BIRC6 Antibody [NB110-40730] - Elevated BIRC6 expression is associated with advanced stages of prostate cancer: co-upregulation of other IAP members. Representative images of correlated expressions between BIRC6 and survivin, XIAP and cIAP1. 20x magnification, scale bar, 100 um. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/2229), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/BIRC6-Antibody-Immunohistochemistry-NB110-40730-img0004.jpg)
![Western Blot: LRPPRC Antibody [NBP1-83349] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LRPPRC antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Western-Blot-NBP1-83349-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: LRPPRC Antibody [NBP1-83349] - Analysis in Hep-G2 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-LRPPRC antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: LRPPRC Antibody [NBP1-83349] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/LRPPRC-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83349-img0007.jpg)
![Western Blot: ATPB Antibody [NBP1-90815] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ATPB-Antibody-Western-Blot-NBP1-90815-img0015.jpg "Western Blot: ATPB Antibody [NBP1-90815] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: ATPB Antibody [NBP1-90815] - Staining of human cell line U-2 OS shows localization to mitochondria. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90815-img0007.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human prostate shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0020.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATPB Antibody [NBP1-90815] - Analysis in A-549 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATP5B antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/ATPB-Antibody-Western-Blot-NBP1-90815-img0009.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human kidney, liver, prostate and small intestine using Anti-ATP5B antibody NBP1-90815 (A) shows similar protein distribution across tissues to independent antibody NBP1-90816 (B).](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0016.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human small intestine shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0018.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0019.jpg)
![Immunohistochemistry-Paraffin: ATPB Antibody [NBP1-90815] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes](http://images.novusbio.com/fullsize/ATPB-Antibody-Immunohistochemistry-Paraffin-NBP1-90815-img0021.jpg)
![Western Blot: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of 75 ug of various whole cell lysates and 10 ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Western-Blot-NBP2-22481-img0001.jpg "Western Blot: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of 75 ug of various whole cell lysates and 10 ul of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel.")
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of LIN28 in NCCIT and HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a LIN28 monoclonal antibody at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunocytochemistry-Immunofluorescence-NBP2-22481-img0005.jpg)
![Immunohistochemistry-Paraffin: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis showing staining in the cytoplasm of human seminoma (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunohistochemistry-Paraffin-NBP2-22481-img0015.jpg)
![Flow Cytometry: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (blue histogram) on H9 embryonic stem cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37C. Cells were incubated with a Lin28 monoclonal antibody or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Flow-Cytometry-NBP2-22481-img0013.jpg)
![Chromatin Immunoprecipitation: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis performed using cross-linked chromatin from rat hepatoma cells treated with insulin. IP performed using a multiplex microplate Matrix ChIP assay of LIN28 monoclonal antibody. Chromatin aliquots from cells were used per ChIP pull-down. Quantitative PCR data done in quadruplicate using 1ul of DNA in 2ul SYBR real-time PCR reactions containing primers to amplify -15kb upstream of Egr1 or exon-1 or exon-2-3 of Egr1. Quantitation of immunoprecipitated chromatin is presented as signal relative to the total amount of input chromatin. Results represent the mean +/- SEM. A schematic representation of the rat Egr-1 locus is shown; oxes represent exons (black boxes = translated, white boxes = untranslated), the zigzag line represents an intron, and the straight line represents upstream sequence. Regions amplified by Egr-1 primers are represented by black bars. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Chromatin-Immunoprecipitation-ChIP-NBP2-22481-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (green) in H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Lin28 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunocytochemistry-Immunofluorescence-NBP2-22481-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (green) in HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Lin28 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBST, and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by fluorescence microscopy at 20X magnification.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunocytochemistry-Immunofluorescence-NBP2-22481-img0004.jpg)
![Immunohistochemistry-Paraffin: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis showing staining in the nucleus and cytoplasm of mouse testis tissue (right) compared with a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunohistochemistry-Paraffin-NBP2-22481-img0014.jpg)
![Flow Cytometry: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of Lin28 (blue histogram) on HEL 11.4 induced IPS cells. To generate single cells suspensions, colonies were treated with TrypLE cell dissociation enzyme for 5 minutes at 37C. Cells were incubated with a Lin28 monoclonal antibody or mouse IgG (green histogram) at a dilution of 1:100 for 1 hour on ice, washed with PBS + 5% fetal calf serum (FACS buffer), and incubated with a fluorescein-conjugated secondary antibody at a dilution of 1:200 for 30 minutes on ice. Cells were washed with cold FACS buffer, resuspended in 500ul of FACS buffer containing 10ul of 4% paraformaldehyde.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Flow-Cytometry-NBP2-22481-img0012.jpg)
![Immunoprecipitation: LIN-28A Antibody (14E6-4E6) [NBP2-22481] - Analysis of LIN28 was performed. Antigen-antibody complexes were formed by incubating 700ug of lysate with 5 ug of an LIN28 monoclonal antibody overnight on a rocking platform at 4C. The immune complexes were captured on 50 ul Protein A/G Agarose was loaded as a positive control for detection. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel, transferred to a PVDF membrane, and blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a LIN28 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4C, washed in TBST, and probed with Clean-blot IP Detection Reagent at a dilution of 1:1000 for at least 1 hour.](http://images.novusbio.com/fullsize/LIN-28A-Antibody-14E6-4E6-Immunoprecipitation-NBP2-22481-img0002.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - Wild-type (WT) and Stathmin 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. CRISPR knockout cell lines generated by Edigene](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-30883] - Wild-type (WT) and Stathmin 1 knockout (KO) HeLa cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. CRISPR knockout cell lines generated by Edigene")
![<b>Genetic Strategies Validation. </b>Western Blot: Stathmin 1 Antibody [NBP1-30883] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Stathmin 1 antibody (NBP1-30883). The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-30883] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Stathmin 1 protein stained by Stathmin 1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:10000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30883-img0007.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - Sample(30 ug of whole cell lysate)A:293TB:A431 C:H129912% SDS PAGE, antibody diluted at 1:3000.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0003.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - A. 50 ug mouse brain lysate/extract 12 % SDS-PAGE Stathmin 1 antibody dilution: 1:10000](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0009.jpg)
![Western Blot: Stathmin 1 Antibody [NBP1-30883] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 12 % SDS-PAGE Stathmin 1 antibody dilution: 1:2000](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Western-Blot-NBP1-30883-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Stathmin 1 Antibody [NBP1-30883] - Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Stathmin 1 protein stained by Stathmin 1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody diluted at 1:1000. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-30883-img0006.jpg)
![Immunohistochemistry-Paraffin: Stathmin 1 Antibody [NBP1-30883] - Sample: Paraffin-embedded human breast carcinoma. Stathmin 1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Stathmin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-30883-img0005.jpg)
![Western Blot: Profilin 1 Antibody [NBP1-19344] - Western Blot on HeLa whole cell extracts.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Western-Blot-NBP1-19344-img0005.jpg "Western Blot: Profilin 1 Antibody [NBP1-19344] - Western Blot on HeLa whole cell extracts.")
![Immunohistochemistry: Profilin 1 Antibody [NBP1-19344] - Staining of Profilin in mouse thymus.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Immunohistochemistry-NBP1-19344-img0004.jpg)
![Simple Western: Profilin 1 Antibody [NBP1-19344] - Simple Western lane view shows a specific band for Profilin 1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-Simple-Western-NBP1-19344-img0006.jpg)
![Western Blot: CLOCK Antibody [NB100-126] - Lanes 3-5 contained alpha CLOCK immunoprecipitated mouse DNA (15, 10 and 5 ul of antisera added respectively to the 900 ul of sonicated chromatin sample in the ChIP assay.](http://images.novusbio.com/fullsize/CLOCK-Antibody-Western-Blot-NB100-126-img0002.jpg "Western Blot: CLOCK Antibody [NB100-126] - Lanes 3-5 contained alpha CLOCK immunoprecipitated mouse DNA (15, 10 and 5 ul of antisera added respectively to the 900 ul of sonicated chromatin sample in the ChIP assay.")
![Western Blot: Nudel Antibody (5E11) [NBP2-01086] Analysis of extracts (35ug) from 9 different cell lines by using anti-Nudel monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Western-Blot-NBP2-01086-img0009.jpg "Western Blot: Nudel Antibody (5E11) [NBP2-01086] Analysis of extracts (35ug) from 9 different cell lines by using anti-Nudel monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: Nudel Antibody (5E11) [NBP2-01086] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nudel.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunocytochemistry-Immunofluorescence-NBP2-01086-img0008.jpg)
![Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human Ovary tissue using anti-Nudel mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunohistochemistry-Paraffin-NBP2-01086-img0007.jpg)
![Flow Cytometry: Nudel Antibody (5E11) [NBP2-01086] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Nudel antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Flow-Cytometry-NBP2-01086-img0001.jpg)
![Western Blot: Nudel Antibody (5E11) [NBP2-01086] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nudel (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nudel.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Western-Blot-NBP2-01086-img0002.jpg)
![Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Nudel mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunohistochemistry-Paraffin-NBP2-01086-img0003.jpg)
![Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Nudel mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunohistochemistry-Paraffin-NBP2-01086-img0004.jpg)
![Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human Kidney tissue using anti-Nudel mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunohistochemistry-Paraffin-NBP2-01086-img0005.jpg)
![Immunohistochemistry-Paraffin: Nudel Antibody (5E11) [NBP2-01086] - Staining of paraffin-embedded Human liver tissue using anti-Nudel mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Nudel-Antibody-5E11-Immunohistochemistry-Paraffin-NBP2-01086-img0006.jpg)