| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59409-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61842-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47607-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61659-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61121-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97471-0.2mg | |||
| BSA Free | NBP1-97471-0.025mg | |||
![Western Blot: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Western blot analysis of various lysates, using Aquaporin-4Rabbit mAb at 1:900 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-912202414154378.jpg "Western Blot: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Western blot analysis of various lysates, using Aquaporin-4Rabbit mAb at 1:900 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-31120251744416.jpg)
![Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-31120251704787.jpg)
![Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-3112025173144.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Human lung cancer tissue using Aquaporin-4 (AQP4) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-immunohistochemistry-paraffin-1352025122932..png)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Western blot analysis of various lysates using Enolase 2/Neuron-specific Enolase Rabbit mAb at 1:10000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Negative control (NC): U-937Exposure time: 15s.](http://images.novusbio.com/fullsize/nbp3-15659_rabbit-enolase-2-neuron-specific-enolase-mab-9b4y4-52202517315564.jpg "Western Blot: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Western blot analysis of various lysates using Enolase 2/Neuron-specific Enolase Rabbit mAb at 1:10000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Negative control (NC): U-937Exposure time: 15s.")
![Immunocytochemistry/ Immunofluorescence: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Confocal imaging of Neuro-2a cells using Enolase 2/Neuron-specific Enolase Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15659_rabbit-enolase-2-neuron-specific-enolase-mab-9b4y4-5220251733389.jpg)
![Immunohistochemistry: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Enolase 2/Neuron-specific Enolase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15659_rabbit-enolase-2-neuron-specific-enolase-mab-9b4y4-52202517361231.jpg)
![Immunohistochemistry: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Enolase 2/Neuron-specific Enolase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15659_rabbit-enolase-2-neuron-specific-enolase-mab-9b4y4-52202517333854.jpg)
![Immunohistochemistry: Enolase 2/Neuron-specific Enolase Antibody (9B4Y4) [Enolase 2/Neuron-specific Enolase] - Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using Enolase 2/Neuron-specific Enolase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15659_rabbit-enolase-2-neuron-specific-enolase-mab-9b4y4-52202517361234.jpg)
![Western Blot: ALDH5A1 Antibody (17F5) [NBP2-59409] - Figure c: Downregulation of the ALDH5A1 protein product SSADH in TG1-miR. Western blot analysis. SSADH MW, 57 kDa; Actin MW, 42 kDa. Mean +/- SD, n = 3 independent biological samples. Figure d: Decreased ALDH5A1 mRNA levels in TG1-miR compared to TG1. Q-PCR assays. Mean +/- SD, n = 3 independent biological samples. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s00401-016-1659-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ALDH5A1-Antibody-17F5-Western-Blot-NBP2-59409-img0005.jpg "<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: ALDH5A1 Antibody (17F5) [NBP2-59409] - Figure c: Downregulation of the ALDH5A1 protein product SSADH in TG1-miR. Western blot analysis. SSADH MW, 57 kDa; Actin MW, 42 kDa. Mean +/- SD, n = 3 independent biological samples. Figure d: Decreased ALDH5A1 mRNA levels in TG1-miR compared to TG1. Q-PCR assays. Mean +/- SD, n = 3 independent biological samples. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s00401-016-1659-5), licensed under a CC-BY license.")
![<b>Biological Strategies Validation and Orthogonal Strategies Validation.</b>Immunocytochemistry/Immunofluorescence: ALDH5A1 Antibody (17F5) [NBP2-59409] - Analysis of ALDH5A1 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDH5A1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/ALDH5A1-Antibody-17F5-Immunocytochemistry-Immunofluorescence-NBP2-59409-img0002.jpg)
![Immunohistochemistry: ALDH5A1 Antibody (17F5) [NBP2-59409] - SSADH immunoreactive cells are enriched in proliferative/non-differentiated GBM territories (PHIGH/D-) and rare in non-proliferative/differentiated (PLOW/D+) tumor territories of patients' GBM, as revealed by immunohistochemical staining. HES: hematoxylin and eosin staining. Scale bar 100 um. Image collected and cropped by CiteAb from the following publication (link.springer.com/10.1007/s00401-016-1659-5), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ALDH5A1-Antibody-17F5-Immunohistochemistry-NBP2-59409-img0004.jpg)
![Western Blot: ALDH5A1 Antibody (17F5) [NBP2-59409] - Analysis in HepG2 cell lysates (40ug), resolved by SDS-PAGE.](http://images.novusbio.com/fullsize/ALDH5A1-Antibody-17F5-Western-Blot-NBP2-59409-img0001.jpg)
![Western Blot: ALDH5A1 Antibody (17F5) [NBP2-59409] - The HepG2 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH5A1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/ALDH5A1-Antibody-17F5-Western-Blot-NBP2-59409-img0003.jpg)
![Western Blot: GSAP Antibody [NBP2-81894] - Analysis of GSAP in EL4 cell lysate with GSAP antibody at 0.25 ug/ml in (A) the absence and (B) presence of blocking peptide.](http://images.novusbio.com/fullsize/GSAP-Antibody-Western-Blot-NBP2-81894-img0001.jpg "Western Blot: GSAP Antibody [NBP2-81894] - Analysis of GSAP in EL4 cell lysate with GSAP antibody at 0.25 ug/ml in (A) the absence and (B) presence of blocking peptide.")
![Immunocytochemistry/ Immunofluorescence: GSAP Antibody - BSA Free [NBP2-81894] - Immunofluorescence of GSAP in mouse brain tissue with GSAP antibody at 20 ug/mL.Red: GSAP Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-81894_rabbit-polyclonal-gsap-antibody-271120241932698.gif)
![Immunocytochemistry/ Immunofluorescence: GSAP Antibody - BSA Free [NBP2-81894] - Immunofluorescence of GSAP in Human Brain cells with GSAP antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81894_rabbit-polyclonal-gsap-antibody-271120242016946.jpg)
![Immunohistochemistry: GSAP Antibody - BSA Free [NBP2-81894] - Immunohistochemistry of GSAP in human brain tissue with GSAP antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp2-81894_rabbit-polyclonal-gsap-antibody-2711202420285841.jpg)
![Immunohistochemistry: GSAP Antibody - BSA Free [NBP2-81894] - Immunohistochemistry of GSAP in mouse brain tissue with GSAP antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-81894_rabbit-polyclonal-gsap-antibody-271120242045679.gif)
![Western Blot: APP Antibody (3A8S2) [NBP3-15560] - Western blot analysis of extracts of various cell lines, using APP antibody (NBP3-15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/APP-Antibody-3A8S2-Western-Blot-NBP3-15560-img0006.jpg "Western Blot: APP Antibody (3A8S2) [NBP3-15560] - Western blot analysis of extracts of various cell lines, using APP antibody (NBP3-15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: APP Antibody (3A8S2) [NBP3-15560] - Immunofluorescence analysis of HeLa cells using APP Rabbit mAb (NBP3-15560) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/APP-Antibody-3A8S2-Immunocytochemistry-Immunofluorescence-NBP3-15560-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: APP Antibody (3A8S2) [NBP3-15560] - Western blot analysis of extracts from normal (control) and APP knockout (KO) 293T cells, using APP antibody (NBP3-15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/APP-Antibody-3A8S2-Knockout-Validated-NBP3-15560-img0001.jpg)
![Immunoprecipitation: APP Antibody (3A8S2) [NBP3-15560] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug APP antibody (NBP3-15560). Western blot was performed from the immunoprecipitate using APP antibody (NBP3-15560) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/APP-Antibody-3A8S2-Immunoprecipitation-NBP3-15560-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: APP Antibody (3A8S2) [APP] - Confocal imaging of paraffin-embedded 5XFAD Mouse brain and Mouse brain using [KO Validated] APP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15560_rabbit-app-mab-3a8s2-52202517304935.jpg)
![Western Blot: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis using GRM3 mAb against HEK293 (1) and GRM3 (AA: extra 433-576)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-Western-Blot-NBP2-61842-img0005.jpg "Western Blot: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis using GRM3 mAb against HEK293 (1) and GRM3 (AA: extra 433-576)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis of paraffin-embedded stomach cancer tissues using GRM3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-Immunohistochemistry-NBP2-61842-img0004.jpg)
![Western Blot: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis using GRM3 mAb against human GRM3 (AA: extra 433-576) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-Western-Blot-NBP2-61842-img0006.jpg)
![Immunohistochemistry: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis of paraffin-embedded bladder cancer tissues using GRM3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-Immunohistochemistry-NBP2-61842-img0003.jpg)
![ELISA: mGluR3 Antibody (6H10C3) [NBP2-61842] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-ELISA-NBP2-61842-img0001.jpg)
![ELISA: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis of SH-SY5Y cells using GRM3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-ELISA-NBP2-61842-img0002.jpg)
![Western Blot: CACNB2 Antibody (N8b/1) [NBP1-47607] - Western Blot analysis of Human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (NBP1-47607). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (NBP1-47607) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/CACNB2-Antibody-N8b-1-Western-Blot-NBP1-47607-img0006.jpg "Western Blot: CACNB2 Antibody (N8b/1) [NBP1-47607] - Western Blot analysis of Human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (NBP1-47607). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (NBP1-47607) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: CACNB2 Antibody (N8b/1) [NBP1-47607] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav beta 2 Monoclonal Antibody, Clone S8B-1 (NBP1-47607). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav beta 2 Monoclonal Antibody (NBP1-47607) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav beta 2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/CACNB2-Antibody-N8b-1-Immunocytochemistry-Immunofluorescence-NBP1-47607-img0008.jpg)
![Immunohistochemistry: CACNB2 Antibody (N8b/1) [NBP1-47607] - Immunohistochemistry analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (NBP1-47607). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (NBP1-47607) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All nuclei. Some nuclei also staining higher up in epidermis.](http://images.novusbio.com/fullsize/CACNB2-Antibody-N8b-1-Immunohistochemistry-NBP1-47607-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CACNB2 Antibody (N8b/1) [NBP1-47607] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive. Bright dottiness located throughout cytoplasm and in nuclei.. Image courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/CACNB2-Antibody-N8b-1-Immunocytochemistry-Immunofluorescence-NBP1-47607-img0003.jpg)
![Western Blot: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis using NEFM mAb against human NEFM (AA: 779-916) recombinant protein. (Expected MW is *** kDa)](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Western-Blot-NBP2-61659-img0004.jpg "Western Blot: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis using NEFM mAb against human NEFM (AA: 779-916) recombinant protein. (Expected MW is *** kDa)")
![Immunocytochemistry/Immunofluorescence: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Hela cells using NEFM mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Immunocytochemistry-Immunofluorescence-NBP2-61659-img0003.jpg)
![Flow Cytometry: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Raji cells using NF-M mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Flow-Cytometry-NBP2-61659-img0007.jpg)
![Western Blot: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis using NEFM mAb against HEK293 (1) and NEFM (AA: 779-916)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Western-Blot-NBP2-61659-img0005.jpg)
![Flow Cytometry: NF-M Antibody (1B6B12) [NBP2-61659] - Analysis of Hela cells using NEFM mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-Flow-Cytometry-NBP2-61659-img0002.jpg)
![ELISA: NF-M Antibody (1B6B12) [NBP2-61659] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-ELISA-NBP2-61659-img0001.jpg)
![ELISA: NF-M Antibody (1B6B12) [NBP2-61659] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).](http://images.novusbio.com/fullsize/NF-M-Antibody-1B6B12-ELISA-NBP2-61659-img0006.jpg)
![Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Immunohistochemistry analysis of rat brain injected with Type 1 mouse alpha synuclein PFFs (NBP2-61596). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 mouse alpha synuclein PFFs (NBP2-61596) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-61596-img0003.jpg "Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Immunohistochemistry analysis of rat brain injected with Type 1 mouse alpha synuclein PFFs (NBP2-61596). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 mouse alpha synuclein PFFs (NBP2-61596) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.")
![In vitro assay: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Active alpha synuclein aggregate seeds the formation of new alpha Synuclein aggregates from the pool of active monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate is combined with 100 uM of active alpha Synuclein monomer, as compared to active alpha Synuclein aggregate and active alpha Synuclein monomer alone. Thioflavin T excitation maximum = 450 nm; emission maximum = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-61596-img0002.jpg)
![Electron Microscopy: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TEM of Type 1 mouse alpha synuclein Pre-formed Fibrils (NBP2-61596). Fibrils were sonicated and image was taken at 100kx magnification.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-61596-img0005.jpg)
![Electron Microscopy: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TEM of Type 1 mouse alpha synuclein Pre-formed Fibrils (NBP2-61596). Image was taken at 100kx magnification.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-61596-img0004.jpg)
![Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TH fluorescence in mouse brain. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-61596_recombinant-mouse-alpha-synuclein-active-pre-formed-fibrils-type-1-protein-immunohistochemistry-205202512497..jpg)
![Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Western-Blot-NBP2-61121-img0004.jpg "Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Phospho serine 129 antibody (NBP2-61121) was used to detect phosphorylated alpha synuclein in primary mouse hippocampal neurons treated with 100 nM sonicated mouse alpha synuclein PFFs (A). Phosphorylated alpha synuclein was visible in perinucleus and neurites compared to untreated control (B). Read the protocol at pabmabs.com/?p=7901. Image courtesy of Trine Rasmussen, Simon Molgaard Jensen at Aarhus University.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0006.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunohistochemistry analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunohistochemistry-NBP2-61121-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 g/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:500 for 24 hours at 4C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/alpha-Synuclein [p Ser129] Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0007.jpg)
![Western Blot: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-OTI1B8-Azide-and-BSA-Free-Western-Blot-NBP2-70854-img0001.jpg "Western Blot: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti")
![Immunohistochemistry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - Staining of paraffin-embedded Human Kidney tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-OTI1B8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70854-img0004.jpg)
![Flow Cytometry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Glutathione Synthetase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-OTI1B8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70854-img0002.jpg)
![Immunohistochemistry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-OTI1B8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70854-img0003.jpg)
![Immunohistochemistry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - Staining of paraffin-embedded Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-OTI1B8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70854-img0005.jpg)
![Western Blot: iNOS Antibody [NBP1-97471] - Rat brain extract, probed with iNOS, pAb.](http://images.novusbio.com/fullsize/iNOS-Antibody-Western-Blot-NBP1-97471-img0001.jpg "Western Blot: iNOS Antibody [NBP1-97471] - Rat brain extract, probed with iNOS, pAb.")