| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44489 | |||
| BSA Free | NBP2-14783-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08901 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20818 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33124 | |||
| IHC-Prediluted | NBP2-48367 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26573-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48364 | |||
| Azide and BSA Free | NBP3-11532 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50050SS | |||
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Analysis in human tonsil and skeletal muscle tissues using NBP2-14783 antibody. Corresponding PTPRC RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/antibody/nbp2-14783_mouse-monoclonal-cd45-antibody-cl0160-immunohistochemistry-paraffin-39202411351..jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Analysis in human tonsil and skeletal muscle tissues using NBP2-14783 antibody. Corresponding PTPRC RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: CD45 Antibody (CL0160) [NBP2-14783] - Western Blot analysis using CD45 antibody (CL0160) [NBP2-14783]. Lane 1: Marker [kDa]. Lane 2: Human cell line Jurkat. Lane 3: Human cell line MCF-7. Observed molecular weight ~230 kDa.](http://images.novusbio.com/fullsize/CD45-Antibody-CL0160-Western-Blot-NBP2-14783-img0007.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] - Staining of human tonsil using CD45 antibody (CL0160) [NBP2-14783] shows strong positivity in a majority of lymphoid cells](http://images.novusbio.com/fullsize/CD45-Antibody-CL0160-Immunohistochemistry-Paraffin-NBP2-14783-img0005.jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Staining of human lymph node shows moderate to strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/antibody/nbp2-14783_mouse-monoclonal-cd45-antibody-cl0160-immunohistochemistry-paraffin-39202411445..jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Staining of human duodenum shows moderate membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/antibody/nbp2-14783_mouse-monoclonal-cd45-antibody-cl0160-immunohistochemistry-paraffin-39202411549..jpg)
![Immunohistochemistry-Paraffin: CD45 Antibody (CL0160) [NBP2-14783] -Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-14783_mouse-monoclonal-cd45-antibody-cl0160-immunohistochemistry-paraffin-39202411643..jpg)
![Western Blot: CD45RA Antibody (K4B5) [NBP3-07159] - Western Blot Analysis of human Spleen tissue lysates using CD45RA Mouse Monoclonal Antibody (K4B5)](http://images.novusbio.com/fullsize/CD45RA-Antibody-K4B5-Western-Blot-NBP3-07159-img0004.jpg "Western Blot: CD45RA Antibody (K4B5) [NBP3-07159] - Western Blot Analysis of human Spleen tissue lysates using CD45RA Mouse Monoclonal Antibody (K4B5)")
![Immunohistochemistry-Paraffin: CD45RA Antibody (K4B5) [NBP3-07159] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD45RA Mouse Monoclonal Antibody (K4B5).](http://images.novusbio.com/fullsize/CD45RA-Antibody-K4B5-Immunohistochemistry-Paraffin-NBP3-07159-img0003.jpg)
![Flow Cytometry: CD45RA Antibody (K4B5) [NBP3-07159] - Flow Cytometric Analysis of Jurkat cells using CD45RA Mouse Monoclonal Antibody (K4B5) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD45RA-Antibody-K4B5-Flow-Cytometry-NBP3-07159-img0001.jpg)
![Immunohistochemistry-Paraffin: CD45RA Antibody (K4B5) [NBP3-07159] - Formalin-fixed, paraffin-embedded human Spleen stained with CD45RA Mouse Monoclonal Antibody (K4B5).](http://images.novusbio.com/fullsize/CD45RA-Antibody-K4B5-Immunohistochemistry-Paraffin-NBP3-07159-img0002.jpg)
![Western Blot: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Western blot analysis of extracts of Mouse brain, using IP3R1 Rabbit mAb (NBP3-16414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/IP3R1-Antibody-4Z1B6-Western-Blot-NBP3-16414-img0005.jpg "Western Blot: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Western blot analysis of extracts of Mouse brain, using IP3R1 Rabbit mAb (NBP3-16414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Immunofluorescence analysis of mouse brain using IP3R1 Rabbit mAb (NBP3-16414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IP3R1-Antibody-4Z1B6-Immunohistochemistry-NBP3-16414-img0004.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Immunohistochemistry of paraffin-embedded rat brain using IP3R1 Rabbit mAb (NBP3-16414) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IP3R1-Antibody-4Z1B6-Immunohistochemistry-Paraffin-NBP3-16414-img0001.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Immunohistochemistry of paraffin-embedded mouse brain using IP3R1 Rabbit mAb (NBP3-16414) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IP3R1-Antibody-4Z1B6-Immunohistochemistry-Paraffin-NBP3-16414-img0002.jpg)
![Immunohistochemistry: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Immunofluorescence analysis of rat brain using IP3R1 Rabbit mAb (NBP3-16414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IP3R1-Antibody-4Z1B6-Immunohistochemistry-NBP3-16414-img0003.jpg)
![Western Blot: xCT Antibody (A7C6-R) [NBP3-33104] - Western blot analysis of xCT on different lysates with Mouse anti-xCT antibody (NBP3-33104) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-288202414191525.jpg "Western Blot: xCT Antibody (A7C6-R) [NBP3-33104] - Western blot analysis of xCT on different lysates with Mouse anti-xCT antibody (NBP3-33104) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: xCT Antibody (A7C6-R) [NBP3-33104] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-xCT antibody (NBP3-33104) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33104) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-298202413585586.jpg)
![Immunocytochemistry/ Immunofluorescence: xCT Antibody (A7C6-R) [NBP3-33104] - Immunocytochemistry analysis of HT-29 cells labeling xCT with Mouse anti-xCT antibody (NBP3-33104) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-xCT antibody (NBP3-33104) at 1/200 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-2920241114813.jpg)
![Flow Cytometry: xCT Antibody (A7C6-R) [NBP3-33104] - Flow cytometric analysis of A549 cells labeling xCT. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-33104, 1ug/ml) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33104_mouse-xct-mab-a7c6-r-29202416372152.jpg)
![Immunocytochemistry/Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204] - Staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red). Cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/AHNAK-Antibody-EM-09-Immunocytochemistry-Immunofluorescence-NBP2-62204-img0002.jpg "Immunocytochemistry/Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204] - Staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red). Cell nuclei stained with DAPI (blue).")
![Immunohistochemistry-Frozen: AHNAK Antibody (EM-09) [NBP2-62204] - Staining (frozen sections) of murine tongue by anti-AHNAK1 antibody (EM-09; red). Actin filaments were decorated by phalloidin (green), cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/AHNAK-Antibody-EM-09-Immunohistochemistry-Frozen-NBP2-62204-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: AHNAK Antibody (EM-09) [NBP2-62204] - Staining of AHNAK1 in human primary fibroblasts using anti-AHNAK1 (EM-09; green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/AHNAK-Antibody-EM-09-Immunocytochemistry-Immunofluorescence-NBP2-62204-img0001.jpg)
![Western Blot: NRG1 Antibody [NBP2-19587] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/NRG1-Antibody-Western-Blot-NBP2-19587-img0001.jpg "Western Blot: NRG1 Antibody [NBP2-19587] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: beta-Catenin Antibody [NBP2-29476] - Western Blot of beta-Catenin (p120) in human brain 1) absence and 2) presence of immunizing peptide and 3) Mouse brain lysate using beta-Catenin PAb.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Western-Blot-NBP2-29476-img0006.jpg "Western Blot: beta-Catenin Antibody [NBP2-29476] - Western Blot of beta-Catenin (p120) in human brain 1) absence and 2) presence of immunizing peptide and 3) Mouse brain lysate using beta-Catenin PAb.")
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP2-29476] - Immunohistochemistry-Paraffin: [NBP2-29476] - Formalin-fixed, paraffin-embedded human breast ductal carcinoma stained with beta- Catenin antibody (1:100), peroxidase-conjugate and Warp Red chromogen (20X). Note specific membrane staining in ductal carcinoma.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP2-29476-img0003.jpg)
![Flow Cytometry: beta-Catenin Antibody [NBP2-29476] - Flow Cytometric Analysis of PFA fixed HeLa cells using beta-Catenin PAb followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Flow-Cytometry-NBP2-29476-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP2-29476] - Formalin-fixed, paraffin-embedded human breast lobular carcinoma stained with beta- Catenin antibody (1:100), peroxidase-conjugate and Warp Red chromogen (20X). Note specific cytoplasmic staining in lobular carcinoma.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-Immunohistochemistry-Paraffin-NBP2-29476-img0002.jpg)
![Immunohistochemistry: c-Fos Antibody (14C10) [NBP3-26573] - Image of c-Fos Antibody (14C10) diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-2722024845324.jpg "Immunohistochemistry: c-Fos Antibody (14C10) [NBP3-26573] - Image of c-Fos Antibody (14C10) diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.")
![Immunohistochemistry: c-Fos Antibody (14C10) [NBP3-26573] - Image of c-Fos Antibody (14C10) diluted at 1:81 and staining in paraffin-embedded human adrenal gland tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-2722024845328.jpg)
![Western Blot: c-Fos Antibody (14C10) [NBP3-26573] - Positive Western Blot detected in: HepG2 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate, 293 whole cell lysate, NIH/3T3 whole cell lysate. All lanes: c-Fos Antibody at 0.81ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 41, 29, 37 KDa Observed band size: 62 KDa](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-282202411351611.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody (14C10) [NBP3-26573] - Staining of HepG2 cells with c-Fos Antibody (14C10) at 1:27, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-28220241249368.jpg)
![Flow Cytometry: c-Fos Antibody (14C10) [NBP3-26573] - Overlay histogram showing Hela cells stained with c-Fos Antibody (14C10) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26573_rabbit-c-fos-mab-14c10-282202414183668.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SPM610) [NBP2-44570] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Antibody (SPM610)](http://images.novusbio.com/fullsize/CD34-Antibody-SPM610-Immunohistochemistry-Paraffin-NBP2-44570-img0002.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (SPM610) [NBP2-44570] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Antibody (SPM610)")
![Immunohistochemistry-Paraffin: CD34 Antibody (SPM610) [NBP2-44570] - Human Angiosarcoma stained with CD34 Monoclonal Antibody (SPM610)](http://images.novusbio.com/fullsize/CD34-Antibody-SPM610-Immunohistochemistry-Paraffin-NBP2-44570-img0001.jpg)
![Western Blot: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of different neuronal tissue lysates using mouse mAb to Calbindin D-28K, dilution 1:5,000: [1] protein standard, [2] rat cerebellum, [3] pig hippocampus, and [4] cow cerebellum. Bands at about 26kDa corresponds to the calbindin protein, heavily expressed in the cerebellum but a very minor component of hippocampus.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Western-Blot-NBP2-50050-img0004.jpg "Western Blot: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of different neuronal tissue lysates using mouse mAb to Calbindin D-28K, dilution 1:5,000: [1] protein standard, [2] rat cerebellum, [3] pig hippocampus, and [4] cow cerebellum. Bands at about 26kDa corresponds to the calbindin protein, heavily expressed in the cerebellum but a very minor component of hippocampus.")
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Adult mouse cortex (Left) and cerebellum (Right) sections were stained with NBP2-50050 in red, and co-stained with rabbit polyclonal anti-RBFOX3/NeuN antibody (NBP1-92716, green). Calbindin is expressed in a subset of interneurons in the cortex (Left) and prominently expressed in the dendrites of Purkinje cells in the cerebellum molecular layer (Right). RBFOX3/NeuN expresses in most neurons; as a result, cells positive for Calbindin appear to be yellow. Insets are high magnification images of the boxed area. Blue is Dapi nucleus staining that labels DNA.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Immunocytochemistry-Immunofluorescence-NBP2-50050-img0003.jpg)
![Immunohistochemistry Free-Floating: Calbindin D-28K Antibody (5A9) [NBP2-50050] - Analysis of rat brain cerebellum section stained with mouse mAb to Calbindin D-28K, dilution 1:2,000 (Red), and costained with rabbit GFAP pAb, dilution 1:5,000 (Green). DAPI staining of nuclear DNA (Blue). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24hrs, cut to 45uM, and free-floating sections were stained with the above antibodies. The Calbindin D-28K antibody prominently labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia in the molecular layer and astroglia in the granular and white matter layers of cerebellum.](http://images.novusbio.com/fullsize/Calbindin-D-28K-Antibody-5A9-Immunohistochemistry-Free-Floating-NBP2-50050-img0006.jpg)
![Western Blot: Brg1 Antibody [NBP2-22234] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Brg1-Antibody-Western-Blot-NBP2-22234-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Brg1 Antibody [NBP2-22234] - Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), Jurkat (50 ug), and mouse NIH3T3 (50 ug) cells. Antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: Brg1 Antibody [NBP2-22234] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- BRG1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunohistochemistry-NBP2-22234-img0014.jpg)
![Immunohistochemistry: Brg1 Antibody [NBP2-22234] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- BRG1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunohistochemistry-NBP2-22234-img0013.jpg)
![Immunoprecipitation: Brg1 Antibody [NBP2-22234] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22234 used for IP at 6 ug/mg lysate. BRG1 was also immunoprecipitated by rabbit anti-BRG1 antibody NB100-2594. For blotting immunoprecipitated BRG1, NBP2-22234 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Brg1-Antibody-Immunoprecipitation-NBP2-22234-img0002.jpg)