| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-20225-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71782 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38030-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52520-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05166SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50031SS | |||
![Western Blot: KCNN3 Antibody [NBP2-85137] - Host: Rabbit. Target Name: KCNN3. Sample Tissue: Human THP-1 Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/KCNN3-Antibody-Western-Blot-NBP2-85137-img0004.jpg "Western Blot: KCNN3 Antibody [NBP2-85137] - Host: Rabbit. Target Name: KCNN3. Sample Tissue: Human THP-1 Whole Cell. Antibody Dilution: 1ug/ml")
![Immunohistochemistry: KCNN3 Antibody [NBP2-85137] - Sample Type: Rhesus macaque spinal cord. Primary Antibody Dilution: 1:300. Secondary Antibody: Donkey anti Rabbit 488. Secondary Antibody Dilution: 1:500. Color/Signal Descriptions: Green: KCNN3. Gene Name: KCNN3. Submitted by: Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School](http://images.novusbio.com/fullsize/KCNN3-Antibody-Immunohistochemistry-NBP2-85137-img0006.jpg)
![Western Blot: KCNN3 Antibody [NBP2-85137] - WB Suggested Anti-KCNN3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Daudi cell lysateKCNN3 is supported by BioGPS gene expression data to be expressed in Daudi](http://images.novusbio.com/fullsize/KCNN3-Antibody-Western-Blot-NBP2-85137-img0002.jpg)
![Western Blot: KCNN3 Antibody [NBP2-85137] - KCNN3 antibody - C-terminal region validated by WB using Mouse kidney, Whole brain lysate at 2 ug/ml.](http://images.novusbio.com/fullsize/KCNN3-Antibody-Western-Blot-NBP2-85137-img0003.jpg)
![Western Blot: KCNN3 Antibody [NBP2-85137] - Host: Rabbit. Target Name: KCNN3. Sample Tissue: Human 293T Whole Cell. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/KCNN3-Antibody-Western-Blot-NBP2-85137-img0005.jpg)
![Immunohistochemistry-Paraffin: KCNN3 Antibody [NBP2-85137] - Rabbit Anti-KCNN3 Antibody. Formalin Fixed Paraffin Embedded Tissue: Human Adult heart. Observed Staining: Membrane. Primary Antibody Concentration: 1:600. Secondary Antibody: Donkey anti-Rabbit-Cy2/3. Secondary Antibody Concentration: 1:200. Magnificatio](http://images.novusbio.com/fullsize/KCNN3-Antibody-Immunohistochemistry-Paraffin-NBP2-85137-img0001.jpg)
![Western Blot: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (NBP3-13688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRK2-p-Ser670-Antibody-HL1035-Western-Blot-NBP3-13688-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (NBP3-13688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - GRK2 (phospho Ser670) antibody [HL1035] detects GRK2 (phospho Ser670) protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. GRK2 (phospho Ser670) stained by GRK2 (phospho Ser670) antibody [HL1035] (NBP3-13688) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GRK2-p-Ser670-Antibody-HL1035-Immunohistochemistry-Paraffin-NBP3-13688-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (NBP3-13688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRK2-p-Ser670-Antibody-HL1035-Western-Blot-NBP3-13688-img0001.jpg)
![Western Blot: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (NBP3-13688) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRK2-p-Ser670-Antibody-HL1035-Western-Blot-NBP3-13688-img0003.jpg)
![Western Blot: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (NBP3-13688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRK2-p-Ser670-Antibody-HL1035-Western-Blot-NBP3-13688-img0004.jpg)
![Western Blot: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - Various tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1035] (NBP3-13688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13688_rabbit-grk2-p-ser-670-mab-hl1035-9102024201574.jpg)
![Western Blot: Neuropeptide Y Antibody [NBP3-13163] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Neuropeptide Y antibody (NBP3-13163) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-Western-Blot-NBP3-13163-img0004.jpg "Western Blot: Neuropeptide Y Antibody [NBP3-13163] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Neuropeptide Y antibody (NBP3-13163) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Neuropeptide Y Antibody [NBP3-13163] - Neuropeptide Y antibody detects Neuropeptide Y protein by immunofluorescent analysis. Sample: DIV10 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Neuropeptide Y stained by Neuropeptide Y antibody (NBP3-13163) diluted at 1:500. Red: Tau, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13163-img0003.jpg)
![Immunohistochemistry-Paraffin: Neuropeptide Y Antibody [NBP3-13163] - Neuropeptide Y antibody detects Neuropeptide Y protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Neuropeptide Y antibody (NBP3-13163) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-Immunohistochemistry-Paraffin-NBP3-13163-img0001.jpg)
![Western Blot: TRPV4 Antibody [NBP1-20225] - WB on mouse brain lysate (reduced, denatured) using Rabbit antibody to TRPV4 at 1 : 1000 dilution.](http://images.novusbio.com/fullsize/TRPV4-Antibody-Western-Blot-NBP1-20225-img0003.jpg "Western Blot: TRPV4 Antibody [NBP1-20225] - WB on mouse brain lysate (reduced, denatured) using Rabbit antibody to TRPV4 at 1 : 1000 dilution.")
![Immunohistochemistry-Paraffin: TRPV4 Antibody [NBP1-20225] - IHC on paraffin sections of rat kidney: Animal was perfused with perfuser at 100 mmHg with 300 ml PBS and then 4%PFA. Tissues were extracted and further fixed for 2 hours at room temperature before being embedded in paraffin. HIER: 1 mM EDTA, pH 8 for 20 min. Detection was done using HRP polymer following manufactures instruction Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)](http://images.novusbio.com/fullsize/TRPV4-Antibody-Immunohistochemistry-Paraffin-NBP1-20225-img0002.jpg)
![Immunohistochemistry-Paraffin: TRPV4 Antibody [NBP1-20225] - IHC on paraffin sections of rat kidney: Animal was perfused with perfuser at 100 mmHg with 300 ml PBS and then 4%PFA. Tissues were extracted and further fixed for 2 hours at room temperature before being embedded in paraffin. HIER: 1 mM EDTA, pH 8 for 20 min. Detection was done using HRP polymer following manufactures instruction Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer)](http://images.novusbio.com/fullsize/TRPV4-Antibody-Immunohistochemistry-Paraffin-NBP1-20225-img0001.jpg)
![Western Blot: APPL Antibody (OTI4B11) [NBP2-46536] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APPL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APPL1.](http://images.novusbio.com/fullsize/APPL-Antibody-OTI4B11-Western-Blot-NBP2-46536-img0001.jpg "Western Blot: APPL Antibody (OTI4B11) [NBP2-46536] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY APPL1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-APPL1.")
![Immunohistochemistry-Paraffin: APPL Antibody (OTI4B11) [NBP2-46536] - Staining of paraffin-embedded Human lymphoma tissue using anti-APPL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/APPL-Antibody-OTI4B11-Immunohistochemistry-Paraffin-NBP2-46536-img0004.jpg)
![Immunohistochemistry-Paraffin: APPL Antibody (OTI4B11) [NBP2-46536] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-APPL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/APPL-Antibody-OTI4B11-Immunohistochemistry-Paraffin-NBP2-46536-img0002.jpg)
![Immunohistochemistry-Paraffin: APPL Antibody (OTI4B11) [NBP2-46536] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-APPL1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/APPL-Antibody-OTI4B11-Immunohistochemistry-Paraffin-NBP2-46536-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP3-38030] - Immunofluorescence analysis of SH-SY5Y cells using PAC1R Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-1012202411531046.jpg "Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP3-38030] - Immunofluorescence analysis of SH-SY5Y cells using PAC1R Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: PAC1R Antibody [NBP3-38030] - Western blot analysis of lysates from Mouse heart , using PAC1R Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-10122024120621.jpg)
![Western Blot: PAC1R Antibody [NBP3-38030] - Western blot analysis of lysates from U-87MG cells, using PAC1R Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-1012202411535820.jpg)
![Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP3-38030] - Immunofluorescence analysis of NIH/3T3 cells using PAC1R Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-10122024120660.jpg)
![Immunocytochemistry/ Immunofluorescence: PAC1R Antibody [NBP3-38030] - Immunofluorescence analysis of C6 cells using PAC1R Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38030_rabbit-pac1r-pab-1012202411535868.jpg)
![Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against human NOS1 (AA: 17-153) recombinant protein. (Expected MW is 40.3 kDa)](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Western-Blot-NBP2-52520-img0003.jpg "Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against human NOS1 (AA: 17-153) recombinant protein. (Expected MW is 40.3 kDa)")
![Immunocytochemistry/Immunofluorescence: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis of HeLa cells using NOS1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Immunofluorescence-NBP2-52520-img0002.jpg)
![Flow Cytometry: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis of HeLa cells using NOS1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Flow-Cytometry-NBP2-52520-img0001.jpg)
![Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against HEK293 (1) and NOS1 (AA: 17-153)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Western-Blot-NBP2-52520-img0004.jpg)
![ELISA: nNOS Antibody (2E11G6) [NBP2-52520] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-ELISA-NBP2-52520-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Testing of tissue from human cerebral cortex, applying ultra thin sections using a technique called array tomography (antibody: green, DAPI-stained nuclei: blue). Image shows labeling of NPY-positive processes in the neutrophil. Image courtesy of Dr. Nancy O'Rourke, Stanford University.](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-8-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05166-img0002.jpg "Immunocytochemistry/Immunofluorescence: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Testing of tissue from human cerebral cortex, applying ultra thin sections using a technique called array tomography (antibody: green, DAPI-stained nuclei: blue). Image shows labeling of NPY-positive processes in the neutrophil. Image courtesy of Dr. Nancy O'Rourke, Stanford University.")
![Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Paraffin embedded mouse brain tissue (interneurons in the cortex) diluted 1/1000](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-8-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05166-img0005.jpg)
![Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Paraffin embedded mouse brain tissue (fibres in the hilus of the hippocampus) diluted 1/1000](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-8-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05166-img0003.jpg)
![Immunohistochemistry-Paraffin: Neuropeptide Y Antibody (8) - BSA Free [NBP1-05166] - Enlarged section of a paraffin embedded mouse brain tissue (fibres in the hilus of the hippocampus) diluted 1/1000](http://images.novusbio.com/fullsize/Neuropeptide-Y-Antibody-8-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05166-img0004.jpg)
![Immunohistochemistry: CNPase Antibody [NB100-1935] - A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunohistochemistry-NB100-1935-img0001.jpg "Immunohistochemistry: CNPase Antibody [NB100-1935] - A tissue section through an adult mouse brain showing CNPase (brown staining) in white matter tracts and the granule cell layer of the cerebellum.")
![CNPase Antibody [NB100-1935] - CNPase-positive oligodendrocytes (brown) in cerebellar white matter (adult mouse). Right: Western.](http://images.novusbio.com/fullsize/CNPase-Antibody-NB100-1935-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: TRPA1 Antibody [NB100-98841] - IF on vagal nodose ganglia. Image from verified customer review.](http://images.novusbio.com/fullsize/TRPA1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-98841-img0001.jpg "Immunocytochemistry/Immunofluorescence: TRPA1 Antibody [NB100-98841] - IF on vagal nodose ganglia. Image from verified customer review.")
![Western Blot: CNPase Antibody (1H10) [NBP2-50031] - Western blot analysis of different tissue lysates using mouse mAb to CNP, NBP2-50031, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. A doublet of bands at 46 and 48kDa correspond to the major isotypes of the CNP protein.](http://images.novusbio.com/fullsize/CNPase-Antibody-1H10-Western-Blot-NBP2-50031-img0004.jpg "Western Blot: CNPase Antibody (1H10) [NBP2-50031] - Western blot analysis of different tissue lysates using mouse mAb to CNP, NBP2-50031, dilution 1:2,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord. A doublet of bands at 46 and 48kDa correspond to the major isotypes of the CNP protein.")
![Immunocytochemistry/Immunofluorescence: CNPase Antibody (1H10) [NBP2-50031] - Mixed neuron-glial cell cultures stained with NBP2-50031 (red) and polyclonal antibody against GFAP (green, NB300-141). NBP2-50031 stains strongly in oligodendrocytes, whereas GFAP labels only the intermediate filaments in astrocytes. Blue is DNA staining.](http://images.novusbio.com/fullsize/CNPase-Antibody-1H10-Immunocytochemistry-Immunofluorescence-NBP2-50031-img0003.jpg)
![Immunohistochemistry Free-Floating: CNPase Antibody (1H10) [NBP2-50031] - Analysis of rat cerebellum section stained with mouse mAb to CNP, NBP2-50031, dilution 1:500 in green and costained with chicken pAb to NF-M, dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The CNP antibody specifically stains oligodendrocytes, cells that create the myelin sheath around axons. NF-M antibody labels axons of neuronal cells.](http://images.novusbio.com/fullsize/CNPase-Antibody-1H10-Immunocytochemistry-Immunofluorescence-NBP2-50031-img0005.jpg)
![Western Blot: SMPD1 Antibody [NBP2-22365] - Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: A431 whole cell lysate |Lysates/proteins at 20 ug per lane. Secondary: IgG (HRP) Goat Anti-Rabbit (1:10000) Predicted band size: 70kD Blocking/Dilution buffer: 5% NFDM/TBST.](http://images.novusbio.com/fullsize/SMPD1-Antibody-Western-Blot-NBP2-22365-img0004.jpg "Western Blot: SMPD1 Antibody [NBP2-22365] - Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: A431 whole cell lysate |Lysates/proteins at 20 ug per lane. Secondary: IgG (HRP) Goat Anti-Rabbit (1:10000) Predicted band size: 70kD Blocking/Dilution buffer: 5% NFDM/TBST.")
![Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365] - Immunohistochemical analysis of paraffin-embedded human breast tissue using SMPD1 antibody (1:500) after HIER using EDTA buffer pH9.0 prior to staining performed on the Leica(R) BOND RXm. An undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody..](http://images.novusbio.com/fullsize/SMPD1-Antibody-Immunohistochemistry-Paraffin-NBP2-22365-img0006.jpg)
![Flow Cytometry: SMPD1 Antibody [NBP2-22365] - Indirect Flow Cytometric analysis of K562 cells (right histogram) using SMPD1 antibody compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.](http://images.novusbio.com/fullsize/SMPD1-Antibody-Flow-Cytometry-NBP2-22365-img0007.jpg)
![Western Blot: SMPD1 Antibody [NBP2-22365] - Lane 1: human cerebellum lysate Lane 2: HepG2 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MCF-7 whole cell lysate Lysates/proteins at 20ug/lane. Secondary: IgG, (H+L) (HRP) goat anti-rabbit (1:10,000). Predicted band size: 70kD |Blocking/Dilution buffer: 5% NFDM/TBST.](http://images.novusbio.com/fullsize/SMPD1-Antibody-Western-Blot-NBP2-22365-img0002.jpg)
![Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365] - Immunohistochemistry analysis of formalin fixed paraffin embedded human testis tissue using SMPD1 antibody (1:500) after HIER using EDTA buffer pH9.0 prior to staining performed on the Leica(R) BOND RXm. An undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody](http://images.novusbio.com/fullsize/SMPD1-Antibody-Immunohistochemistry-Paraffin-NBP2-22365-img0005.jpg)