| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18568-25ul | |||
| Azide and BSA Free | NBP3-44383 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88182-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61828-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-30599SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88604-25ul | |||
![Immunohistochemistry-Paraffin: L1CAM Antibody (CL12984) [NBP3-18568] - Staining of human kidney shows strong membranous positivity in a subset of cells in tubules.](http://images.novusbio.com/fullsize/L1CAM-Antibody-CL12984-Immunohistochemistry-Paraffin-NBP3-18568-img0008.jpg "Immunohistochemistry-Paraffin: L1CAM Antibody (CL12984) [NBP3-18568] - Staining of human kidney shows strong membranous positivity in a subset of cells in tubules.")
![Immunohistochemistry-Paraffin: L1CAM Antibody (CL12984) [NBP3-18568] - Analysis in human cerebral cortex and testis tissues using NBP3-18568 antibody. Corresponding L1CAM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/L1CAM-Antibody-CL12984-Immunohistochemistry-Paraffin-NBP3-18568-img0001.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (CL12984) [NBP3-18568] - Staining of human ovarian cancer (high grade serous carcinoma) shows moderate membranous positivity in a subset of tumor cells.](http://images.novusbio.com/fullsize/L1CAM-Antibody-CL12984-Immunohistochemistry-Paraffin-NBP3-18568-img0002.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (CL12984) [NBP3-18568] - Staining of human ovarian cancer (clear cell carcinoma) shows moderate membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/L1CAM-Antibody-CL12984-Immunohistochemistry-Paraffin-NBP3-18568-img0003.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (CL12984) [NBP3-18568] - Staining of human ovarian cancer (papillary carcinoma) shows moderate membranous positivity in a subset of tumor cells.](http://images.novusbio.com/fullsize/L1CAM-Antibody-CL12984-Immunohistochemistry-Paraffin-NBP3-18568-img0004.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (CL12984) [NBP3-18568] - Staining of human cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/L1CAM-Antibody-CL12984-Immunohistochemistry-Paraffin-NBP3-18568-img0005.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (CL12984) [NBP3-18568] - Staining of human cerebellum shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/L1CAM-Antibody-CL12984-Immunohistochemistry-Paraffin-NBP3-18568-img0006.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (CL12984) [NBP3-18568] - Staining of human small intestine shows strong positivity in peripheral ganglion and neural fibers.](http://images.novusbio.com/fullsize/L1CAM-Antibody-CL12984-Immunohistochemistry-Paraffin-NBP3-18568-img0007.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (CL12984) [NBP3-18568] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/L1CAM-Antibody-CL12984-Immunohistochemistry-Paraffin-NBP3-18568-img0009.jpg)
![Immunohistochemistry-Paraffin: Caspr2 Antibody [NBP1-88182] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Caspr2-Antibody-Immunohistochemistry-Paraffin-NBP1-88182-img0007.jpg)
![Western Blot: Caspr2 Antibody [NBP1-88182] - Analysis in control (vector only transfected HEK293T lysate) and CNTNAP2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Caspr2-Antibody-Western-Blot-NBP1-88182-img0009.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1333] - Human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1333-img0005.jpg "Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1333] - Human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1333] - Analysis of brain, neurons and glia using this antibody at 6 ug/ ml.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1333-img0001.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1333] - Analysis of anti-CHRM2 / M2 antibody with human brain, glioblastoma.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1333-img0002.jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody [NLS1333] - Human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-Immunohistochemistry-Paraffin-NLS1333-img0004.jpg)
![Western Blot: TMEM59 Antibody [NBP1-32303] - Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000](http://images.novusbio.com/fullsize/TMEM59-Antibody-Western-Blot-NBP1-32303-img0018.jpg "Western Blot: TMEM59 Antibody [NBP1-32303] - Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000")
![Immunohistochemistry-Paraffin: TMEM59 Antibody [NBP1-32303] - Mouse lung. TMEM59 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TMEM59-Antibody-Immunohistochemistry-Paraffin-NBP1-32303-img0014.jpg)
![Western Blot: TMEM59 Antibody [NBP1-32303] - Sample (30 ug of whole cell lysate) A:A43112% SDS PAGE diluted at 1:500](http://images.novusbio.com/fullsize/TMEM59-Antibody-Western-Blot-NBP1-32303-img0020.jpg)
![Western Blot: TMEM59 Antibody [NBP1-32303] - A. 50 ug rat kidney lysate/extract 10 % SDS-PAGETMEM59 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/TMEM59-Antibody-Western-Blot-NBP1-32303-img0017.jpg)
![Immunohistochemistry-Paraffin: TMEM59 Antibody [NBP1-32303] - Human ovarian carcinoma. TMEM59 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TMEM59-Antibody-Immunohistochemistry-Paraffin-NBP1-32303-img0015.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Wild-type (WT) and beta-III Tubulin knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta-III Tubulin antibody [GT1338] detects beta-III Tubulin protein at cytoplasm by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: beta-III Tubulin protein stained by beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:500. Red: NeuN, stained by NeuN antibody diluted at 1:1000. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Immunocytochemistry-Immunofluorescence-NBP3-13505-img0002.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - beta Tubulin 3/ TUJ1 antibody detects beta Tubulin 3/ TUJ1 protein by Western blot analysis. Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta Tubulin 3/ TUJ1 antibody (NBP3-13505) diluted by 1:1000.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0001.jpg)
![Western Blot: beta-III Tubulin Antibody (GT1338) [NBP3-13505] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with beta-III Tubulin antibody [GT1338] (NBP3-13505) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-GT1338-Western-Blot-NBP3-13505-img0003.jpg)
![beta Tubulin 3/ Tuj1 antibody [GT1338] detects beta Tubulin 3/ Tuj1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse E10.5 embryo.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-13505_mouse-beta-iii-tubulin-mab-gt1338-immunohistochemistry-paraffin-2611202518393116.jpg)
![Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Western blot analysis of extracts of various cell lines, using Muscarinic AChR M2 (Muscarinic Acetylcholine Receptor M2/CHRM2) Rabbit mAb (NBP3-16576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Muscarinic-Acetylcholine-Receptor-M2-CHRM2-Antibody-3T5W8-Western-Blot-NBP3-16576-img0004.jpg "Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Western blot analysis of extracts of various cell lines, using Muscarinic AChR M2 (Muscarinic Acetylcholine Receptor M2/CHRM2) Rabbit mAb (NBP3-16576) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Analysis of paraffin-embedded Human esophageal cancer using Muscarinic AChR M2 (Muscarinic AChR M2 (ACM2)) Rabbit mAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-16576_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-3t5w8-immunohistochemistry-paraffin-3152024111317..jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Human tonsil tissue using Muscarinic AChR M2 (ACM2) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16576_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-3t5w8-immunohistochemistry-paraffin-3152024111413..jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Mouse kidney tissue using Muscarinic AChR M2 (ACM2) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16576_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-3t5w8-immunohistochemistry-paraffin-315202411152..jpg)
![Immunohistochemistry-Paraffin: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Mouse testis tissue using Muscarinic AChR M2 (ACM2) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16576_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-3t5w8-immunohistochemistry-paraffin-315202411165..jpg)
![Immunohistochemistry: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (3T5W8) [NBP3-16576] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Muscarinic AChR M2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16576_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-3t5w8-52202511402786.jpg)
![Western Blot: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis using GRM5 mAb against human GRM5 (AA: extra 458-580) recombinant protein. (Expected MW is 40.1 kDa)](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-Western-Blot-NBP2-61828-img0005.jpg "Western Blot: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis using GRM5 mAb against human GRM5 (AA: extra 458-580) recombinant protein. (Expected MW is 40.1 kDa)")
![Immunocytochemistry/Immunofluorescence: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis of Hela cells using GRM5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-Immunocytochemistry-Immunofluorescence-NBP2-61828-img0002.jpg)
![Immunohistochemistry: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis of paraffin-embedded stomach cancer tissues using GRM5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-Immunohistochemistry-NBP2-61828-img0004.jpg)
![Western Blot: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis using GRM5 mAb against HEK293 (1) and GRM5 (AA: extra 458-580)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-Western-Blot-NBP2-61828-img0006.jpg)
![Immunohistochemistry: mGluR5 Antibody (1A11D10) [NBP2-61828] - Analysis of paraffin-embedded bladder cancer tissues using GRM5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-Immunohistochemistry-NBP2-61828-img0003.jpg)
![ELISA: mGluR5 Antibody (1A11D10) [NBP2-61828] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/mGluR5-Antibody-1A11D10-ELISA-NBP2-61828-img0001.jpg)
![Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (JE38-35) [NBP3-32617] - Western blot analysis of Muscarinic Acetylcholine Receptor M2/CHRM2 on different lysates with Rabbit anti-Muscarinic Acetylcholine Receptor M2/CHRM2 antibody (NBP3-32617) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: U-87 MG cell lysate Lane 4: Neuro-2a cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32617) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32617_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-27820241631631.jpg "Western Blot: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (JE38-35) [NBP3-32617] - Western blot analysis of Muscarinic Acetylcholine Receptor M2/CHRM2 on different lysates with Rabbit anti-Muscarinic Acetylcholine Receptor M2/CHRM2 antibody (NBP3-32617) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: U-87 MG cell lysate Lane 4: Neuro-2a cell lysate Lane 5: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 1 minute 17 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32617) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (JE38-35) [NBP3-32617] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Muscarinic Acetylcholine Receptor M2/CHRM2 antibody (NBP3-32617) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32617) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32617_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-29820241435280.jpg)
![Immunocytochemistry/ Immunofluorescence: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (JE38-35) [NBP3-32617] - Immunocytochemistry analysis of SH-SY5Y cells labeling Muscarinic Acetylcholine Receptor M2/CHRM2 with Rabbit anti-Muscarinic Acetylcholine Receptor M2/CHRM2 antibody (NBP3-32617) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Muscarinic Acetylcholine Receptor M2/CHRM2 antibody (NBP3-32617) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃.Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32617_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-29202410484825.jpg)
![Flow Cytometry: Muscarinic Acetylcholine Receptor M2/CHRM2 Antibody (JE38-35) [NBP3-32617] - Flow cytometric analysis of SH-SY5Y cells labeling Muscarinic Acetylcholine Receptor M2/CHRM2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32617, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32617_rabbit-muscarinic-acetylcholine-receptor-m2-chrm2-mab-392024884617.jpg)
![Western Blot: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-28820241423313.jpg "Western Blot: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: PC-12 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: Daudi cell lysate Lane 5: Jurkat cell lysate Lane 6: A431 cell lysate Lane 7: K-562 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 1 minutes 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33139) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33139) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-29820241435223.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33139) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-29202410484898.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-94) [NBP3-33139] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33139, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33139_rabbit-alpha-tubulin-mab-psh02-94-39202488464.jpg)
![Western Blot: PAFAH1B3 Antibody (8C11) [H00005050-M02] - PAFAH1B3 monoclonal antibody (M02), clone 8C11. Analysis of PAFAH1B3 expression in Jurkat.](http://images.novusbio.com/fullsize/PAFAH1B3-Antibody-8C11-Western-Blot-H00005050-M02-img0011.jpg "Western Blot: PAFAH1B3 Antibody (8C11) [H00005050-M02] - PAFAH1B3 monoclonal antibody (M02), clone 8C11. Analysis of PAFAH1B3 expression in Jurkat.")
![Immunocytochemistry/Immunofluorescence: PAFAH1B3 Antibody (8C11) [H00005050-M02] - Analysis of monoclonal antibody to PAFAH1B3 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PAFAH1B3-Antibody-8C11-Immunocytochemistry-Immunofluorescence-H00005050-M02-img0007.jpg)
![Western Blot: PAFAH1B3 Antibody (8C11) [H00005050-M02] - PAFAH1B3 monoclonal antibody (M02), clone 8C11. Analysis of PAFAH1B3 expression in PC-12.](http://images.novusbio.com/fullsize/PAFAH1B3-Antibody-8C11-Western-Blot-H00005050-M02-img0009.jpg)
![Western Blot: PAFAH1B3 Antibody (8C11) [H00005050-M02] - PAFAH1B3 monoclonal antibody (M02), clone 8C11 Analysis of PAFAH1B3 expression in IMR-32.](http://images.novusbio.com/fullsize/PAFAH1B3-Antibody-8C11-Western-Blot-H00005050-M02-img0010.jpg)
![Sandwich ELISA: PAFAH1B3 Antibody (8C11) [H00005050-M02] - Detection limit for recombinant GST tagged PAFAH1B3 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PAFAH1B3-Antibody-8C11-Sandwich-ELISA-H00005050-M02-img0008.jpg)
![Immunohistochemistry-Paraffin: IGSF4B/SynCAM3/CADM3 Antibody [NBP1-88604] - Analysis in human cerebral cortex and pancreas tissues. Corresponding CADM3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/IGSF4B-SynCAM3-CADM3-Antibody-Immunohistochemistry-Paraffin-NBP1-88604-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: IGSF4B/SynCAM3/CADM3 Antibody [NBP1-88604] - Analysis in human cerebral cortex and pancreas tissues. Corresponding CADM3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: IGSF4B/SynCAM3/CADM3 Antibody [NBP1-88604] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/IGSF4B-SynCAM3-CADM3-Antibody-Western-Blot-NBP1-88604-img0005.jpg)
![Immunohistochemistry-Paraffin: IGSF4B/SynCAM3/CADM3 Antibody [NBP1-88604] - Staining of human rectum shows strong positivity in peripheral nerve / ganglion.](http://images.novusbio.com/fullsize/IGSF4B-SynCAM3-CADM3-Antibody-Immunohistochemistry-Paraffin-NBP1-88604-img0014.jpg)
![Immunohistochemistry-Paraffin: IGSF4B/SynCAM3/CADM3 Antibody [NBP1-88604] - Staining of human cerebellum shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/IGSF4B-SynCAM3-CADM3-Antibody-Immunohistochemistry-Paraffin-NBP1-88604-img0011.jpg)
![Immunohistochemistry-Paraffin: IGSF4B/SynCAM3/CADM3 Antibody [NBP1-88604] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/IGSF4B-SynCAM3-CADM3-Antibody-Immunohistochemistry-Paraffin-NBP1-88604-img0012.jpg)
![Immunohistochemistry-Paraffin: IGSF4B/SynCAM3/CADM3 Antibody [NBP1-88604] - Staining of human pancreas shows low positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/IGSF4B-SynCAM3-CADM3-Antibody-Immunohistochemistry-Paraffin-NBP1-88604-img0013.jpg)