| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33498-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74344 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08936 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21180-25ul | |||
| Azide and BSA Free | NBP3-44262 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41264-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73526 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97494-0.2mg | |||
| BSA Free | NBP1-97494-0.025mg | |||
![Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Analysis in human bone marrow and cerebral cortex tissues. Corresponding Cathepsin G RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Analysis in human bone marrow and cerebral cortex tissues. Corresponding Cathepsin G RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0007.jpg)
![Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0001.jpg)
![Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0005.jpg)
![Immunohistochemistry-Paraffin: Cathepsin G Antibody [NBP2-33498] - Staining of human rectum shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Cathepsin-G-Antibody-Immunohistochemistry-Paraffin-NBP2-33498-img0006.jpg)
![Western Blot: SPR Antibody (4F5) [NBP2-03256] Analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Western-Blot-NBP2-03256-img0013.jpg "Western Blot: SPR Antibody (4F5) [NBP2-03256] Analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: SPR Antibody (4F5) [NBP2-03256] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunocytochemistry-Immunofluorescence-NBP2-03256-img0012.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human pancreas tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0011.jpg)
![Flow Cytometry: SPR Antibody (4F5) [NBP2-03256] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SPR antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Flow-Cytometry-NBP2-03256-img0002.jpg)
![Western Blot: SPR Antibody (4F5) [NBP2-03256] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Western-Blot-NBP2-03256-img0003.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0004.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0005.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0006.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0007.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0008.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human Kidney tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0009.jpg)
![Immunohistochemistry-Paraffin: SPR Antibody (4F5) [NBP2-03256] - Staining of paraffin-embedded Human liver tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Immunohistochemistry-Paraffin-NBP2-03256-img0010.jpg)
![Flow Cytometry: SPR Antibody (4F5) [NBP2-03256] - Analysis of Jurkat cells, using anti-SPR antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPR-Antibody-4F5-Flow-Cytometry-NBP2-03256-img0001.jpg)
![Western Blot: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Western-Blot-NBP2-74344-img0002.jpg "Western Blot: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR.")
![Immunocytochemistry/Immunofluorescence: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74344-img0005.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Human liver tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0012.jpg)
![Flow Cytometry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-SPR antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74344-img0004.jpg)
![Western Blot: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Western-Blot-NBP2-74344-img0001.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0006.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0007.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0008.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0009.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0010.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Human Kidney tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0011.jpg)
![Immunohistochemistry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Staining of paraffin-embedded Human pancreas tissue using anti-SPR mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74344-img0013.jpg)
![Flow Cytometry: SPR Antibody (OTI4F5) - Azide and BSA Free [NBP2-74344] - Analysis of Jurkat cells, using anti-SPR antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPR-Antibody-OTI4F5-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74344-img0003.jpg)
![Western Blot: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis using HMGB1 antibody. Human ThP1 cell lysate in the (1) absence and (2) presence of immunizing peptide, as well as (3) mouse NIH 3T3 cell lysate were probed using HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Western-Blot-NBP2-27117-img0008.jpg "Western Blot: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis using HMGB1 antibody. Human ThP1 cell lysate in the (1) absence and (2) presence of immunizing peptide, as well as (3) mouse NIH 3T3 cell lysate were probed using HMGB1 antibody at 5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody [NBP2-27117] - Analysis of decalcified, FFPE tissue sections from the paws of mice with collagen-induced arthritis using the HMGB1 antibody. The antibody was used at 5 ug/ml. Data courtesy of Dr Ulf Andersson, Karolinska Institute.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Immunohistochemistry-Paraffin-NBP2-27117-img0007.jpg)
![Flow Cytometry: HMGB1/HMG-1 Antibody [NBP2-27117] - Intracellular analysis using HMGB1 antibody. Human monocytes were probed using 0.5 ug of HMGB1 antibody (red) and 0.5 ug of isotype control antibody (green). NBP2-27117 and APC secondary this antibody were used for this test.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Flow-Cytometry-NBP2-27117-img0006.jpg)
![Simple Western: HMGB1/HMG-1 Antibody [NBP2-27117] - Lane view shows a specific band for HMGB1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-Simple-Western-NBP2-27117-img0005.jpg)
![Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Western-Blot-NBP3-07191-img0005.jpg "Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human tonsil stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0003.jpg)
![Western Blot: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Western Blot Analysis of human A375 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Western-Blot-NBP3-07191-img0004.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human melanoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0001.jpg)
![Immunohistochemistry-Paraffin: Vimentin Antibody (VIM/1937R) [NBP3-07191] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).](http://images.novusbio.com/fullsize/Vimentin-Antibody-VIM-1937R-Immunohistochemistry-Paraffin-NBP3-07191-img0002.jpg)
![Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - KO Validation in HeLa Cells.Loading: 10 u of HeLa WT cell lysates or HMGB1/HMG-1 KO cell lysates. Antibodies: HMGB1/HMG-1 (0.5 u/mL) and beta-actin (1 u/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-271120241932699.gif "<b>Genetic Strategies Validation. </b>Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - KO Validation in HeLa Cells.Loading: 10 u of HeLa WT cell lysates or HMGB1/HMG-1 KO cell lysates. Antibodies: HMGB1/HMG-1 (0.5 u/mL) and beta-actin (1 u/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation in Mouse Tissues.Loading: 15 ul of lysate Antibodies: HMGB1/HMG-1 , 1 ug/mL , 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420405564.gif)
![Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation in Rat Tissues.Loading: 15 ug of lysate Antibodies: HMGB1/HMG-1, 1 u/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-271120242074672.gif)
![Western Blot: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation in Human, Mouse and Rat Cell Lines.Loading: 15 ug of lysate Antibodies: HMGB1/HMG-1, 1 u/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420392765.gif)
![Immunocytochemistry/ Immunofluorescence: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in HL60 Cells.Immunofluorescent analysis of 4% paraformaldehyde-fixed HL60 cells labeling HMGB1/HMG-1 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI antibody (blue).](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202419432287.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in Human Stomach Tissue.Immunohistochemical analysis of paraffin-embedded human stomach tissue using anti-HMGB1/HMG-1 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420312979.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in Mouse Spleen Tissue.Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-HMGB1/HMG-1 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420392762.jpg)
![Immunohistochemistry: HMGB1/HMG-1 Antibody - BSA Free [NBP2-41264] - Validation of HMGB1/HMG-1 in Rat Spleen Tissue. Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-HMGB1/HMG-1 antibody at 1 ul/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp2-41264_rabbit-polyclonal-hmgb1-hmg-1-antibody-2711202420333866.jpg)
![Western Blot: PON1 Antibody (OTI2D4) [NBP2-03706] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PON1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PON1.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Western-Blot-NBP2-03706-img0004.jpg "Western Blot: PON1 Antibody (OTI2D4) [NBP2-03706] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PON1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PON1.")
![Immunocytochemistry/Immunofluorescence: PON1 Antibody (OTI2D4) [NBP2-03706] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PON1.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Immunocytochemistry-Immunofluorescence-NBP2-03706-img0008.jpg)
![Immunohistochemistry-Paraffin: PON1 Antibody (OTI2D4) [NBP2-03706] - Staining of paraffin-embedded Human prostate tissue using anti-PON1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Immunohistochemistry-Paraffin-NBP2-03706-img0007.jpg)
![Flow Cytometry: PON1 Antibody (OTI2D4) [NBP2-03706] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PON1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Flow-Cytometry-NBP2-03706-img0003.jpg)
![Immunohistochemistry-Paraffin: PON1 Antibody (OTI2D4) [NBP2-03706] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PON1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Immunohistochemistry-Paraffin-NBP2-03706-img0005.jpg)
![Immunohistochemistry-Paraffin: PON1 Antibody (OTI2D4) [NBP2-03706] - Staining of paraffin-embedded Human endometrium tissue using anti-PON1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Immunohistochemistry-Paraffin-NBP2-03706-img0006.jpg)
![Flow Cytometry: PON1 Antibody (OTI2D4) [NBP2-03706] - Analysis of Hela cells, using anti-PON1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Flow-Cytometry-NBP2-03706-img0001.jpg)
![Flow Cytometry: PON1 Antibody (OTI2D4) [NBP2-03706] - Analysis of Jurkat cells, using anti-PON1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Flow-Cytometry-NBP2-03706-img0002.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Analysis of extracts of HepG2 cells, using Alpha-2-Macroglobulin (alpha 2-Macroglobulin) Rabbit mAb (NBP3-16875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-7V6M10-Western-Blot-NBP3-16875-img0007.jpg "Western Blot: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Analysis of extracts of HepG2 cells, using Alpha-2-Macroglobulin (alpha 2-Macroglobulin) Rabbit mAb (NBP3-16875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Human cervix cancer tissue using Alpha-2-Macroglobulin (A2M) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-immunohistochemistry-paraffin-1110202485424..jpg)
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Human tonsil tissue using Alpha-2-Macroglobulin (A2M) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-immunohistochemistry-paraffin-1110202485548..jpg)
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Mouse brain tissue using Alpha-2-Macroglobulin (A2M) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-immunohistochemistry-paraffin-111020248578..jpg)
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Rat spleen tissue using Alpha-2-Macroglobulin (A2M) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-immunohistochemistry-paraffin-111020248589..jpg)
![Immunocytochemistry/ Immunofluorescence: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Immunofluorescence analysis of paraffin-embedded human liver using Alpha-2-Macroglobulin ) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-5220251136127.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Immunofluorescence analysis of paraffin-embedded mouse liver using Alpha-2-Macroglobulin ) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-52202511365123.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Immunofluorescence analysis of paraffin-embedded rat liver using Alpha-2-Macroglobulin ) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-52202511383776.jpg)
![Immunohistochemistry: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Alpha-2-Macroglobulin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-52202511365143.jpg)
![Western Blot: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PON1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PON1.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Azide-and-BSA-Free-Western-Blot-NBP2-73526-img0001.jpg "Western Blot: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PON1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PON1.")
![Immunocytochemistry/Immunofluorescence: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PON1.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-73526-img0005.jpg)
![Immunohistochemistry: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - Staining of paraffin-embedded Human endometrium tissue using anti-PON1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73526-img0007.jpg)
![Flow Cytometry: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PON1 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73526-img0004.jpg)
![Immunohistochemistry: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PON1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73526-img0006.jpg)
![Immunohistochemistry: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - Staining of paraffin-embedded Human prostate tissue using anti-PON1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73526-img0008.jpg)
![Flow Cytometry: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - Analysis of Hela cells, using anti-PON1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73526-img0002.jpg)
![Flow Cytometry: PON1 Antibody (OTI2D4) - Azide and BSA Free [NBP2-73526] - Analysis of Jurkat cells, using anti-PON1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PON1-Antibody-OTI2D4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-73526-img0003.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) [NBP1-97494] - Crystallin AB Antibody (1B6.1-3G4) Lane 1: MW marker, Lane 2: alpha-Crystallin, Lane 3: alpha A Crystallin, Lane 4: alpha B Crystallin, Lane 5: alpha-Crystallin.](http://images.novusbio.com/fullsize/Crystallin-AB-Antibody-1B6-1-3G4-Western-Blot-NBP1-97494-img0004.jpg "Western Blot: AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) [NBP1-97494] - Crystallin AB Antibody (1B6.1-3G4) Lane 1: MW marker, Lane 2: alpha-Crystallin, Lane 3: alpha A Crystallin, Lane 4: alpha B Crystallin, Lane 5: alpha-Crystallin.")
![Immunohistochemistry-Paraffin: AlphaB Crystallin/CRYAB Antibody (1B6.1-3G4) [NBP1-97494] - Crystallin AB Antibody (1B6.1-3G4) Human Heart tissue stained with alpha B-Crystallin, mAb (1B6.1-3G4) at 10ug/ml.](http://images.novusbio.com/fullsize/Crystallin-AB-Antibody-1B6-1-3G4-Immunohistochemistry-Paraffin-NBP1-97494-img0003.jpg)
![Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024158265.jpg "Immunoprecipitation: Vimentin Antibody (SC60-05) [NBP3-33094] - Vimentin was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-33094 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-33094 at 1/10,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-33094 in HeLa cell lysate Lane 3: NBP3-33094 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 5 seconds")
![Immunohistochemistry-Frozen: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunofluorescence analysis of frozen mouse hippocampus tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094).The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33094, green) at 1/100 dilution overnight at 4℃, washed with PBS. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-2682024159390.jpg)
![Immunohistochemistry: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33094) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-298202414161822.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (SC60-05) [NBP3-33094] - Immunocytochemistry analysis of C6 cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202410234261.jpg)
![Flow Cytometry: Vimentin Antibody (SC60-05) [NBP3-33094] - Flow cytometric analysis of HeLa (positive, red) and MCF7 (negative, green) cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33094) at 1/1,000 dilution, compared with Rabbit IgG Isotype Control (HeLa black, MCF7 light green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-29202416453228.jpg)
![Western Blot: Vimentin Antibody (SC60-05) [NBP3-33094] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33094) at 1/20,000 dilution. Lane 1: HeLa cell lysate (10 µg/Lane) Lane 2: HEK-293 cell lysate (10 µg/Lane) Lane 3: Jurkat cell lysate (10 µg/Lane) Lane 4: C2C12 cell lysate (10 µg/Lane) Lane 5: RAW264.7 cell lysate (10 µg/Lane) Lane 6: C6 cell lysate (10 µg/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/20,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33094_rabbit-vimentin-mab-sc60-05-3920248453527.jpg)