| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70508 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71097 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50033SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43907 | |||
| BSA Free | NBP2-52866-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43957 | |||
| BSA Free | NBP2-59778-25ul | |||
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NBP1-31258-img0009.jpg "Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.")
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Paraffin-embedded endometrial, using antibody at 1:150 dilution.](http://images.novusbio.com/fullsize/GPER-GPR30-Antibody-Immunohistochemistry-Paraffin-NBP1-31258-img0008.jpg)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - GPER/GPR30 antibody [C2C3], C-term detects GPER/GPR30 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.GPER/GPR30 stained by GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-91020242001028.jpg)
![Immunocytochemistry/Immunofluorescence: GPER/GPR30 Antibody [NBP1-31258] - DIV11 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GPR30 stained by GPR30 antibody [C2C3], C-term diluted at 1:100.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [11710] diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunocytochemistry-immunofluorescence-16122024114732..png)
![Immunohistochemistry-Paraffin: GPER/GPR30 Antibody [NBP1-31258] - Mouse tissues.GPR30 stained by GPR30 antibody [C2C3], C-term diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/antibody/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunohistochemistry-paraffin-1612202411499..png)
![<b>Orthogonal Strategies Validation. </b>Boiled and unboiled AsPC-1 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-western-blot-1411202518352213.jpg)
![<b>Orthogonal Strategies Validation. </b>Boiled and unboiled various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-western-blot-1411202518344912.jpg)
![GPER/GPR30 antibody [C2C3], C-term detects GPER/GPR30 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.Green: GPER/GPR30 stained by GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunocytochemistry-immunofluorescence-1411202518434418.jpg)
![<b>Orthogonal Strategies Validation. </b>Non-transfected (-) and transfected (+) bolied and unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPER/GPR30 antibody [C2C3], C-term (NBP1-31258) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-western-blot-1411202518344921.jpg)
![GPER/GPR30 antibody detects GPER/GPR30 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GPER/GPR30 stained by GPER/GPR30 antibody (NBP1-31258) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-31258_rabbit-polyclonal-gper-gpr30-antibody-immunocytochemistry-immunofluorescence-141120251841523.jpg)
![Western Blot: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Western-Blot-NBP2-46400-img0008.jpg "Western Blot: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Immunohistochemistry-NBP2-46400-img0007.jpg)
![Western Blot: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Western-Blot-NBP2-46400-img0009.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Immunohistochemistry-NBP2-46400-img0003.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Immunohistochemistry-NBP2-46400-img0004.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Immunohistochemistry-NBP2-46400-img0005.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Immunohistochemistry-NBP2-46400-img0006.jpg)
![Immunofluorescence: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY beta-Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Immunofluorescence-NBP2-46400-img0001.jpg)
![Immunofluorescence: beta-Catenin Antibody (OTI9C1) [NBP2-46400] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Immunofluorescence-NBP2-46400-img0002.jpg)
![Western Blot: L1CAM Antibody (OTI2A6) [NBP2-02149] - Analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Western-Blot-NBP2-02149-img0012.jpg "Western Blot: L1CAM Antibody (OTI2A6) [NBP2-02149] - Analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunocytochemistry-Immunofluorescence-NBP2-02149-img0015.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded pancreas tissue using anti-L1CAMmouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunohistochemistry-Paraffin-NBP2-02149-img0010.jpg)
![Flow Cytometry: L1CAM Antibody (OTI2A6) [NBP2-02149] - Analysis of Jurkat cells, using anti-L1CAM antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Flow-Cytometry-NBP2-02149-img0002.jpg)
![Western Blot: L1CAM Antibody (OTI2A6) [NBP2-02149] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Western-Blot-NBP2-02149-img0003.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded endometrium tissue within the normal limits using anti-L1CAM mouse monoclonal antibody. Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunohistochemistry-Paraffin-NBP2-02149-img0017.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-L1CAMmouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunohistochemistry-Paraffin-NBP2-02149-img0004.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-L1CAM mouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunohistochemistry-Paraffin-NBP2-02149-img0005.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded breast tissue using anti-L1CAM mouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunohistochemistry-Paraffin-NBP2-02149-img0006.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded colon tissue using anti-L1CAM mouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunohistochemistry-Paraffin-NBP2-02149-img0007.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded Kidney tissue using anti-L1CAM mouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunohistochemistry-Paraffin-NBP2-02149-img0008.jpg)
![Immunohistochemistry-Paraffin: L1CAM Antibody (OTI2A6) [NBP2-02149] - Staining of paraffin-embedded Ovary tissue using anti-L1CAM mouse monoclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunohistochemistry-Paraffin-NBP2-02149-img0009.jpg)
![Flow Cytometry: L1CAM Antibody (OTI2A6) [NBP2-02149] - Analysis of Hela cells, using anti-L1CAM antibody, (Red) compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Flow-Cytometry-NBP2-02149-img0001.jpg)
![Immunoprecipitation: L1CAM Antibody (OTI2A6) [NBP2-02149] - Immunoprecipitation of L1CAM by using TrueMab monoclonal anti-L1CAM antibody (Negative control: IP without adding anti-L1CAM antibody). For each experiment, 500ul of DDK tagged L1CAM overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-L1CAM antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.](http://images.novusbio.com/fullsize/L1CAM-Antibody-OTI2A6-Immunoprecipitation-NBP2-02149-img0018.jpg)
![Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of Mouse brain, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Western-Blot-NBP3-15842-img0008.jpg "Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of Mouse brain, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts of HeLa cells, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Western-Blot-NBP3-15842-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Western blot analysis of extracts from normal (control) and beta-Catenin knockout (KO) 293T cells, using beta-Catenin antibody (NBP3-15842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Knockout-Validated-NBP3-15842-img0001.jpg)
![Immunoprecipitation: beta-Catenin Antibody (9L6X0) [NBP3-15842] - Immunoprecipitation analysis of 600ug extracts of Mouse brain using 3ug beta-Catenin antibody (NBP3-15842). Western blot was performed from the immunoprecipitate using beta-Catenin (NBP3-15842) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-9L6X0-Immunoprecipitation-NBP3-15842-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Human colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517284937.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human liver using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517184515.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517162378.jpg)
![Western Blot: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Western blot analysis of lysates from NIH/3T3 cells, using [KO Validated] beta-Catenin Rabbit mAb at 1:1000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517223995.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Western blot analysis of lysates from wild type(WT) and beta-Catenin knockout (KO) 293T(KO) cells, using [KO Validated] beta-Catenin Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517202968.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-522025171893.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Mouse colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517223986.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human solitary fibrous tumor tissue using [KO Validated] beta-Catenin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M Tris-EDTA buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517172874.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Human prostate cancer tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-5220251718979.jpg)
![Immunohistochemistry: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using [KO Validated] beta-Catenin Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517273553.jpg)
![Immunocytochemistry/ Immunofluorescence: beta-Catenin Antibody (6A3P2) [beta-Catenin] - Confocal imaging of paraffin-embedded Rat colon tissue using [KO Validated] beta-Catenin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15842_rabbit-beta-catenin-mab-6a3p2-52202517284993.jpg)
![Western Blot: N-Cadherin Antibody (6F9G8) [NBP3-15654] - Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (NBP3-15654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](http://images.novusbio.com/fullsize/N-Cadherin-Antibody-6F9G8-Knockout-Validated-NBP3-15654-img0001.jpg "<b>Genetic Strategies Validation. </b>Western Blot: N-Cadherin Antibody (6F9G8) [NBP3-15654] - Western blot analysis of extracts from normal (control) and N-Cadherin knockout (KO) HeLa cells, using N-Cadherin antibody (NBP3-15654) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Rat heart tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517311049.jpg)
![Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Confocal imaging of paraffin-embedded rat heart using [KO Validated] N-Cadherin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517352755.jpg)
![Western Blot: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Western blot analysis of lysates from C2C12 cells using [KO Validated] N-Cadherin Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517304949.jpg)
![Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-522025173458.jpg)
![Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Human liver tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517344234.jpg)
![Immunocytochemistry/ Immunofluorescence: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Confocal imaging of paraffin-embedded Mouse heart using [KO Validated] N-Cadherin Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. High pressure antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517311076.jpg)
![Immunohistochemistry: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using [KO Validated] N-Cadherin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517361268.jpg)
![Western Blot: N-Cadherin Antibody (6F9G8) [N-Cadherin] - Western blot analysis of lysates from C6 cells using [KO Validated] N-Cadherin Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15654_rabbit-n-cadherin-mab-6f9g8-52202517311081.jpg)
![Western Blot: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Western-Blot-NBP2-70508-img0002.jpg "Western Blot: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY beta-Catenin.")
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY beta-Catenin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70508-img0008.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0007.jpg)
![Western Blot: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Western-Blot-NBP2-70508-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70508-img0009.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0003.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0004.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0005.jpg)
![Immunohistochemistry: beta-Catenin Antibody (OTI9C1) - Azide and BSA Free [NBP2-70508] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-OTI9C1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70508-img0006.jpg)
![Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Western-Blot-NBP2-67129-img0011.jpg "Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0009.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse heart tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0008.jpg)
![Flow Cytometry: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of Jurkat cells with EDG1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Flow-Cytometry-NBP2-67129-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0003.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0004.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human kidney tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0005.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human liver tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0006.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse brain tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0007.jpg)
![Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on HT-29 cell lysates with Rabbit anti-Beta-Catenin antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Western-Blot-NBP2-67307-img0009.jpg "Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on HT-29 cell lysates with Rabbit anti-Beta-Catenin antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Usage of Beta Catenin antibody in ICC of HT-29 cells](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Immunocytochemistry-NBP2-67307-img0004.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling Beta-Catenin with Rabbit anti-Beta-Catenin antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Immunohistochemistry-Paraffin-NBP2-67307-img0010.jpg)
![Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on different cell lysates using anti- Beta-Catenin antibody at 1/1,000 dilution. Positive control: Lane 1: SW480 Lane 2: HT29 Lane 3: A431](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Western-Blot-NBP2-67307-img0007.jpg)
![Western Blot: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of Beta-Catenin on different lysates with Rabbit anti-Beta-Catenin antibody at 1/500 dilution. Lane 1: SW480 cell lysate Lane 2: A431 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 85 kDa Observed band size: 85 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Western-Blot-NBP2-67307-img0008.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of mouse small intestine tissue using anti-b-Catenin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Immunohistochemistry-Paraffin-NBP2-67307-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of mouse colon tissue using anti- Beta-Catenin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Immunohistochemistry-Paraffin-NBP2-67307-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (SA30-04) [NBP2-67307] - Analysis of human colon cancer tissue using anti- Beta-Catenin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-SA30-04-Immunohistochemistry-Paraffin-NBP2-67307-img0006.jpg)
![Western Blot: ALDH1L1 Antibody (2E7) [NBP2-50033] - Analysis of different tissue and cell lysates using mouse mAb to ALDH1L1, NBP2-50033, dilution 1:5,000 in green: [1] protein standard (red), rat tissue lysates: [2] heart, [3] liver, [4] kidney,[5] lung, [6] brain, and [7] spinal cord; mouse tissue lysates: [8] brain, and [9] spinal cord; cell lysates: [10] NIH-3T3, and [11] HEK293. The band at 100kDa mark corresponds to ALDH1L1 protein.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-2E7-Western-Blot-NBP2-50033-img0003.jpg "Western Blot: ALDH1L1 Antibody (2E7) [NBP2-50033] - Analysis of different tissue and cell lysates using mouse mAb to ALDH1L1, NBP2-50033, dilution 1:5,000 in green: [1] protein standard (red), rat tissue lysates: [2] heart, [3] liver, [4] kidney,[5] lung, [6] brain, and [7] spinal cord; mouse tissue lysates: [8] brain, and [9] spinal cord; cell lysates: [10] NIH-3T3, and [11] HEK293. The band at 100kDa mark corresponds to ALDH1L1 protein.")
![Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to aldehyde dehydrogenase 1L1 (ALDH1L1), NBP2-50033, dilution 1:1,000 in red, and costained with chicken pAb to GFAP, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. NBP2-50033 antibody produces cytoplasmic staining of glial cells, while the GFAP antibody labels the intermediate filament cytoskeleton in astrocytes and other glial cells. Some astrocytic cells express both ALDH1L1 and GFAP and appear yellow.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-2E7-Immunocytochemistry-Immunofluorescence-NBP2-50033-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033] - Neuron-glia cell mixed cultures stained with NBP2-50033 (red) and chicken polyclonal antibody against Vimentin (NB300-223, green). Blue is a DNA stain. NBP2-50033 stains astrocytic cell bodies and processes. The fibroblastic cells contain only Vimentin and so are green, while astrocytes contain either Vimentin and ALDH1L1, so appearing golden, or predominantly ALDH1L1, in which case they appear red.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-2E7-Immunocytochemistry-Immunofluorescence-NBP2-50033-img0001.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in human endometrium and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in human endometrium and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866] - Analysis of extracts from RH-30 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-ZEB1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensi](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Western-Blot-NBP2-52866-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in RH30 cell line with Anti-ZEB1 monoclonal antibody, showing nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunocytochemistry-Immunofluorescence-NBP2-52866-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Analysis in human endometrium and liver tissues using AMAb90510 antibody. Corresponding ZEB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0013.jpg)
![Western Blot: ZEB1 Antibody (CL0151) [NBP2-52866] - Lane 1: Marker [kDa] Lane 2: Human cell line RH-30 Lane 3: Human cell line CACO-2](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Western-Blot-NBP2-52866-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining in A549 cell line with Anti-ZEB1 monoclonal antibody, showing nuclear (without nucleoli) staining in green. Microtubule probes are visualized in red (where available). Antibody stianing shown in green.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunocytochemistry-Immunofluorescence-NBP2-52866-img0001.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0009.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human endometrium shows strong nuclear positivity in stromal cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0010.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (CL0151) [NBP2-52866] - Staining of human smooth muscle shows strong nuclear positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/ZEB1-Antibody-CL0151-Immunohistochemistry-Paraffin-NBP2-52866-img0011.jpg)
![Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778] - Profilin 1 Antibody (5216) [NBP2-59778] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-PFN1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-5216-Immunohistochemistry-Paraffin-NBP2-59778-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778] - Profilin 1 Antibody (5216) [NBP2-59778] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-PFN1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human testis shows weak immunoreactivity in basal cells of the seminiferous ducts.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-5216-Immunohistochemistry-Paraffin-NBP2-59778-img0005.jpg)
![Western Blot: Profilin 1 Antibody (5216) [NBP2-59778] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-5216-Western-Blot-NBP2-59778-img0006.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human fallopian tube shows cytoplasmic immunoreactivity in epithelial cells.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-5216-Immunohistochemistry-Paraffin-NBP2-59778-img0001.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human tonsil shows moderate to strong immunoreactivity in lymphoid cells.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-5216-Immunohistochemistry-Paraffin-NBP2-59778-img0002.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human liver shows strong immunoreactivity in circulating lymphoid cells.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-5216-Immunohistochemistry-Paraffin-NBP2-59778-img0003.jpg)
![Immunohistochemistry-Paraffin: Profilin 1 Antibody (5216) [NBP2-59778] - Staining of human kidney shows strong membranous combined with moderate cytoplasmic immunoreactivity in renal tubules.](http://images.novusbio.com/fullsize/Profilin-1-Antibody-5216-Immunohistochemistry-Paraffin-NBP2-59778-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Profilin 1 Antibody (CL3524) [NBP2-59778] - Lysates of HAP1 WT and PFN1 KO were prepared, and 50 ug of protein were processed for immunoblot with Profilin 1 antibody (NBP2-59778). The Ponceau stained transfer of the blot is shown. Antibody dilution used: NBP2-59778 at 1/1000. Predicted band size: 15 kDa. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/Profilin-1-Antibody-CL3524-Knockout-Validated-NBP2-59778-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: TAX1BP1 Antibody [NBP1-86662] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/TAX1BP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86662-img0005.jpg "Immunocytochemistry/Immunofluorescence: TAX1BP1 Antibody [NBP1-86662] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: TAX1BP1 Antibody [NBP1-86662] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/TAX1BP1-Antibody-Immunohistochemistry-Paraffin-NBP1-86662-img0007.jpg)
![Immunohistochemistry-Paraffin: TAX1BP1 Antibody [NBP1-86662] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TAX1BP1-Antibody-Immunohistochemistry-Paraffin-NBP1-86662-img0006.jpg)
![Immunohistochemistry-Paraffin: TAX1BP1 Antibody [NBP1-86662] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TAX1BP1-Antibody-Immunohistochemistry-Paraffin-NBP1-86662-img0008.jpg)