| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59341-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22420-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12913-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59379-25ug | |||
![Western Blot: Filamin A Antibody (3X8Y9) [NBP3-16266] - Western blot analysis of extracts of various cell lines, using Filamin A Rabbit mAb (NBP3-16266) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Filamin-A-Antibody-3X8Y9-Western-Blot-NBP3-16266-img0004.jpg "Western Blot: Filamin A Antibody (3X8Y9) [NBP3-16266] - Western blot analysis of extracts of various cell lines, using Filamin A Rabbit mAb (NBP3-16266) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: Filamin A Antibody (3X8Y9) [NBP3-16266] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Filamin A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16266_rabbit-filamin-a-mab-3x8y9-52202511392272.jpg)
![Immunohistochemistry: Filamin A Antibody (3X8Y9) [NBP3-16266] - Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue tissue using Filamin A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16266_rabbit-filamin-a-mab-3x8y9-52202511361268.jpg)
![Immunohistochemistry: Filamin A Antibody (3X8Y9) [NBP3-16266] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using Filamin A Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16266_rabbit-filamin-a-mab-3x8y9-52202511361276.jpg)
![Immunohistochemistry: Filamin A Antibody (3X8Y9) [NBP3-16266] - Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Filamin A Rabbit mAb at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16266_rabbit-filamin-a-mab-3x8y9-5220251242539.jpg)
![Western Blot: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Analysis of extracts of various cell lines, using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-8T4D3-Western-Blot-NBP3-15454-img0002.jpg "Western Blot: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Analysis of extracts of various cell lines, using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Immunofluorescence analysis of Hep G2 cells using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-8T4D3-Immunocytochemistry-Immunofluorescence-NBP3-15454-img0001.jpg)
![Western Blot: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Analysis of extracts of various cell lines, using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-8T4D3-Western-Blot-NBP3-15454-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog/Shh Antibody (8T4D3) [Sonic Hedgehog/Shh] - Confocal imaging of paraffin-embedded Mouse kidney using Sonic Hedgehog/Shh Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15454_rabbit-sonic-hedgehog-shh-mab-8t4d3-522025171899.jpg)
![Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog/Shh Antibody (8T4D3) [Sonic Hedgehog/Shh] - Confocal imaging of Hep G2 cells using Sonic Hedgehog/Shh Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15454_rabbit-sonic-hedgehog-shh-mab-8t4d3-52202517192951.jpg)
![Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Western Blot analysis of Human thyroid lysate showing detection of SLC5A5/Sodium Iodide Symporter protein using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-14F-Western-Blot-NBP2-59341-img0009.jpg "Western Blot: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Western Blot analysis of Human thyroid lysate showing detection of SLC5A5/Sodium Iodide Symporter protein using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000.")
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-14F-Immunohistochemistry-NBP2-59341-img0012.jpg)
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-14F-Immunohistochemistry-NBP2-59341-img0010.jpg)
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Tissue: Trachea. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-14F-Immunohistochemistry-NBP2-59341-img0011.jpg)
![Immunohistochemistry: SLC5A5/Sodium Iodide Symporter Antibody (14F) [NBP2-59341] - Immunohistochemistry analysis using Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody, Clone 14F (NBP2-59341). Tissue: Thyroid. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-SLC5A5/Sodium Iodide Symporter Monoclonal Antibody (NBP2-59341) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/SLC5A5-Sodium-Iodide-Symporter-Antibody-14F-Immunohistochemistry-NBP2-59341-img0013.jpg)
![Western Blot: SYT7 Antibody (S275-14) [NBP2-22420] - Western Blot analysis of Rat brain lysates showing detection of SYT7 protein using Mouse Anti-SYT7 Monoclonal Antibody, Clone S275-14 (NBP2-22420). Primary Antibody: Mouse Anti-SYT7 Monoclonal Antibody (NBP2-22420) at 1:100, 1:250, 1:500, and 1:1000.](http://images.novusbio.com/fullsize/SYT7-Antibody-S275-14-Western-Blot-NBP2-22420-img0001.jpg "Western Blot: SYT7 Antibody (S275-14) [NBP2-22420] - Western Blot analysis of Rat brain lysates showing detection of SYT7 protein using Mouse Anti-SYT7 Monoclonal Antibody, Clone S275-14 (NBP2-22420). Primary Antibody: Mouse Anti-SYT7 Monoclonal Antibody (NBP2-22420) at 1:100, 1:250, 1:500, and 1:1000.")
![Immunocytochemistry/Immunofluorescence: SYT7 Antibody (S275-14) [NBP2-22420] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Synaptotagmin-7 Monoclonal Antibody, Clone S275-14 (NBP2-22420). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Synaptotagmin-7 Monoclonal Antibody (NBP2-22420) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Synaptotagmin-7 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SYT7-Antibody-S275-14-Immunocytochemistry-Immunofluorescence-NBP2-22420-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SYT7 Antibody (S275-14) [NBP2-22420] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-7 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/SYT7-Antibody-S275-14-Immunocytochemistry-Immunofluorescence-NBP2-22420-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: SYT7 Antibody (S275-14) [NBP2-22420] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Synaptotagmin-7 Monoclonal Antibody, Clone S275-14 (NBP2-22420). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Synaptotagmin-7 Monoclonal Antibody (NBP2-22420) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasmic Vesicle, Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Synaptotagmin-7 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/SYT7-Antibody-S275-14-Immunocytochemistry-Immunofluorescence-NBP2-22420-img0004.jpg)
![Western Blot: SHANK2 Antibody (N23b/49) [NBP2-12913] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody (NBP2-12913) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/SHANK2-Antibody-N23b-49-Western-Blot-NBP2-12913-img0003.jpg "Western Blot: SHANK2 Antibody (N23b/49) [NBP2-12913] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody (NBP2-12913) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 (pan) Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK2 (pan) Monoclonal Antibody (NBP2-12913) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK2 (pan) Antibody (D) Composite.](http://images.novusbio.com/fullsize/SHANK2-Antibody-N23b-49-Immunocytochemistry-Immunofluorescence-NBP2-12913-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 (pan) Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK2 (pan) Monoclonal Antibody (NBP2-12913) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK2 (pan) Antibody. (D) Composite.](http://images.novusbio.com/fullsize/SHANK2-Antibody-N23b-49-Immunocytochemistry-Immunofluorescence-NBP2-12913-img0005.jpg)
![Western Blot: CLCN3 Antibody (S258-5) [NBP2-59379] - Western Blot analysis of Rat Brain Membrane showing detection of ~90 kDa CIcn3 protein using Mouse Anti-CIcn3 Monoclonal Antibody, Clone S258-5 (NBP2-59379). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-CIcn3 Monoclonal Antibody (NBP2-59379) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa.](http://images.novusbio.com/fullsize/CLCN3-Antibody-S258-5-Western-Blot-NBP2-59379-img0005.jpg "Western Blot: CLCN3 Antibody (S258-5) [NBP2-59379] - Western Blot analysis of Rat Brain Membrane showing detection of ~90 kDa CIcn3 protein using Mouse Anti-CIcn3 Monoclonal Antibody, Clone S258-5 (NBP2-59379). Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-CIcn3 Monoclonal Antibody (NBP2-59379) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~90 kDa.")
![Immunocytochemistry/Immunofluorescence: CLCN3 Antibody (S258-5) [NBP2-59379] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Clcn3 Monoclonal Antibody, Clone S258-5 (NBP2-59379). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Clcn3 Monoclonal Antibody (NBP2-59379) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Clcn3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/CLCN3-Antibody-S258-5-Immunocytochemistry-Immunofluorescence-NBP2-59379-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CLCN3 Antibody (S258-5) [NBP2-59379] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Clcn3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane, Endosome, Endosome membrane, Cytoplasmic Vesicle, Secretory Vesicle Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/CLCN3-Antibody-S258-5-Immunocytochemistry-Immunofluorescence-NBP2-59379-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CLCN3 Antibody (S258-5) [NBP2-59379] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Clcn3 Monoclonal Antibody, Clone S258-5 (NBP2-59379). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Clcn3 Monoclonal Antibody (NBP2-59379) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Membrane, Endosome, Endosome membrane, Cytoplasmic Vesicle, Secretory Vesicle Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Clcn3 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/CLCN3-Antibody-S258-5-Immunocytochemistry-Immunofluorescence-NBP2-59379-img0007.jpg)
![Western Blot: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Syntenin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Syntenin 1.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-OTI2H6-Azide-and-BSA-Free-Western-Blot-NBP2-74418-img0002.jpg "Western Blot: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Syntenin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Syntenin 1.")
![Immunohistochemistry: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Syntenin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-OTI2H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74418-img0004.jpg)
![Western Blot: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-Syntenin 1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-OTI2H6-Azide-and-BSA-Free-Western-Blot-NBP2-74418-img0001.jpg)
![Immunohistochemistry: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Syntenin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-OTI2H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74418-img0003.jpg)
![Immunohistochemistry: Syntenin 1 Antibody (OTI2H6) - Azide and BSA Free [NBP2-74418] - Staining of paraffin-embedded Human liver tissue using anti-Syntenin 1 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Syntenin-1-Antibody-OTI2H6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74418-img0005.jpg)
![Western Blot: HDAC6 Antibody (236) [NBP3-05295] - Western blotting analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 ug/ml), followed by HRP-conjugated anti-mouse secondary antibody.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Western-Blot-NBP3-05295-img0004.jpg "Western Blot: HDAC6 Antibody (236) [NBP3-05295] - Western blotting analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295 in HEK-293T cell line under reducing conditions. PVDF membrane was blocked in 5% milk, and incubated with primary antibody (1 ug/ml), followed by HRP-conjugated anti-mouse secondary antibody.")
![Immunocytochemistry/Immunofluorescence: HDAC6 Antibody (236) [NBP3-05295] - Immunocytochemistry staining of HDAC6 in formaldehyde-fixed and Triton-permeabilized HEK-293T cells (A) and SH-SY5Y cells (B) by mouse monoclonal antibody NBP3-05295, followed by anti-mouse Alexa Fluor 488 (green), DNA indicated by DAPI (blue). In SH-SY5Y cells tubulin was stained by a rabbit polyclonal antibody, followed by anti-rabbit Alexa Fluor 647 (red, colocalization yellow).](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Immunocytochemistry-Immunofluorescence-NBP3-05295-img0001.jpg)
![ELISA: HDAC6 Antibody (236) [NBP3-05295] - ELISA analysis of human HDAC6 by mouse monoclonal antibody NBP3-05295. Plate was coated with recombinant human HDAC6, then blocked with BSA, and exposed to dilution series of anti-HDAC6 primary antibody, followed by HRP-conjugated anti-mouse secondary antibody, and colorimetric signal of processed OPD substrate was measured at 492 nm.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-ELISA-NBP3-05295-img0002.jpg)
![Immunoprecipitation: HDAC6 Antibody (236) [NBP3-05295] - Immunoprecipitation of recombinant human HDAC6 by mouse monoclonal antibodies 178 and NBP3-05295 using protein G-coated Dynabeads. Eluted proteins were processed by SDS PAAGE under reducing conditions and visualized by Coomassie Brilliant Blue.](http://images.novusbio.com/fullsize/HDAC6-Antibody-236-Immunoprecipitation-NBP3-05295-img0003.jpg)
![Flow Cytometry: Mouse Monoclonal HDAC6 Antibody (236) [NBP3-05295] - Separation of K562 cells stained using anti-human HDAC6 (236) purified antibody (concentration in sample 5.33 ug/ml, GAM APC, red-filled) from K562 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).](http://images.novusbio.com/fullsize/antibody/nbp3-05295_mouse-monoclonal-hdac6-antibody-236-flow-cytometry-1112202412584..jpg)
![Western Blot: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Western blot analysis of Cytokeratin 19 on different lysates with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 40 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32247) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32247_mouse-cytokeratin-19-mab-a3d1-r-278202415242660.jpg "Western Blot: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Western blot analysis of Cytokeratin 19 on different lysates with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/1,000 dilution. Lane 1: SK-Br-3 cell lysate Lane 2: MCF7 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 40 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32247) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32247) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32247_mouse-cytokeratin-19-mab-a3d1-r-298202413585528.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Immunocytochemistry analysis of MCF7 cells labeling Cytokeratin 19 with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Cytokeratin 19 antibody (NBP3-32247) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32247_mouse-cytokeratin-19-mab-a3d1-r-29202410334065.jpg)
![Flow Cytometry: Cytokeratin 19 Antibody (A3D1-R) [NBP3-32247] - Flow cytometric analysis of MCF7 cells labeling Cytokeratin 19. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32247, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32247_mouse-cytokeratin-19-mab-a3d1-r-39202488462.jpg)
![Immunocytochemistry/Immunofluorescence: Nav1.7 Antibody [NBP2-75581] - Staining NaV1.7 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Nav1.7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75581-img0004.jpg "Immunocytochemistry/Immunofluorescence: Nav1.7 Antibody [NBP2-75581] - Staining NaV1.7 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: Nav1.7 Antibody [NBP2-75581] - Analysis of paraffin-embedded mouse colon tissue using anti-NaV1.7 beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Nav1.7-Antibody-Immunohistochemistry-Paraffin-NBP2-75581-img0006.jpg)
![Flow Cytometry: Nav1.7 Antibody [NBP2-75581] - Analysis of SH-SY5Y cells with NaV1.7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/Nav1.7-Antibody-Flow-Cytometry-NBP2-75581-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Nav1.7 Antibody [NBP2-75581] - Staining NaV1.7 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Nav1.7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75581-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Nav1.7 Antibody [NBP2-75581] - Staining NaV1.7 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Nav1.7-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-75581-img0003.jpg)
![Immunohistochemistry-Paraffin: Nav1.7 Antibody [NBP2-75581] - Analysis of paraffin-embedded human liver cancer tissue using anti-NaV1.7 beta antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Nav1.7-Antibody-Immunohistochemistry-Paraffin-NBP2-75581-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Immunofluorescence staining of PFA-fixed HeLa cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-mouse IgG-CF488 (green).](http://images.novusbio.com/fullsize/CCR5-Antibody-12D1-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47759-img0002.jpg "Immunocytochemistry/Immunofluorescence: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Immunofluorescence staining of PFA-fixed HeLa cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-mouse IgG-CF488 (green).")
![Immunohistochemistry-Paraffin: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Human Stomach tissue stained with CCR5 Antibody (12D1).](http://images.novusbio.com/fullsize/CCR5-Antibody-12D1-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47759-img0001.jpg)
![Flow Cytometry: CCR5 Antibody (12D1) - Azide and BSA Free [NBP2-47759] - Flow Cytometric Analysis of MCF-7 cells using CCR5 Mouse Monoclonal Antibody (12D1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CCR5-Antibody-12D1-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47759-img0003.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1507) - Azide and BSA Free [NBP2-54542] - beta-Catenin Antibody (CTNNB1/1507) [NBP2-54542] - Formalin-fixed, paraffin-embedded Mouse Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1507).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1507-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54542-img0008.jpg "Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1507) - Azide and BSA Free [NBP2-54542] - beta-Catenin Antibody (CTNNB1/1507) [NBP2-54542] - Formalin-fixed, paraffin-embedded Mouse Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1507).")
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1507) - Azide and BSA Free [NBP2-54542] - beta-Catenin Antibody (CTNNB1/1507) [NBP2-54542] - Formalin-fixed, paraffin-embedded human Pancreas stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1507).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1507-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54542-img0005.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1507) - Azide and BSA Free [NBP2-54542] - beta-Catenin Antibody (CTNNB1/1507) [NBP2-54542] - Formalin-fixed, paraffin-embedded human Tonsil stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1507).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1507-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54542-img0006.jpg)
![Immunohistochemistry-Paraffin: beta-Catenin Antibody (CTNNB1/1507) - Azide and BSA Free [NBP2-54542] - beta-Catenin Antibody (CTNNB1/1507) [NBP2-54542] - Formalin-fixed, paraffin-embedded Rat Colon stained with Beta-Catenin Monoclonal Antibody (CTNNB1/1507).](http://images.novusbio.com/fullsize/beta-Catenin-Antibody-CTNNB1-1507-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54542-img0007.jpg)