| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93205-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94362-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92798-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12602-50ul | |||
![Western Blot: SPAG16 Antibody [NBP2-93205] - Analysis of extracts of mouse testis, using SPAG16 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/SPAG16-Antibody-Western-Blot-NBP2-93205-img0003.jpg "Western Blot: SPAG16 Antibody [NBP2-93205] - Analysis of extracts of mouse testis, using SPAG16 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Western Blot: Nudel Antibody (9C8T10) [NBP3-16275] - Western blot analysis of extracts of various cell lines, using Nudel Rabbit mAb (NBP3-16275) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/Nudel-Antibody-9C8T10-Western-Blot-NBP3-16275-img0001.jpg "Western Blot: Nudel Antibody (9C8T10) [NBP3-16275] - Western blot analysis of extracts of various cell lines, using Nudel Rabbit mAb (NBP3-16275) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: Myt1 Antibody [NBP2-94362] - Analysis of extracts of various cell lines, using Myt1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.](http://images.novusbio.com/fullsize/Myt1-Antibody-Western-Blot-NBP2-94362-img0001.jpg "Western Blot: Myt1 Antibody [NBP2-94362] - Analysis of extracts of various cell lines, using Myt1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.")
![Western Blot: PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5) [NBP3-16930] - Western blot analysis of extracts of various cell lines, using PAK1/2/3 Rabbit mAb (NBP3-16930) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PAK1-2-3-p-Ser141-p-Ser139-p-Ser144-Antibody-1T7A5-Western-Blot-NBP3-16930-img0001.jpg "Western Blot: PAK1/2/3 [p Ser141, p Ser139, p Ser144] Antibody (1T7A5) [NBP3-16930] - Western blot analysis of extracts of various cell lines, using PAK1/2/3 Rabbit mAb (NBP3-16930) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37C for 30 minutes after serum-starvation overnight Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: PPP2R2A Antibody (9E5N2) [NBP3-33476] - Western blot analysis of lysates from HeLa cells, using PPP2R2A Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33476_rabbit-ppp2r2a-mab-9e5n2-912202415161827.jpg "Western Blot: PPP2R2A Antibody (9E5N2) [NBP3-33476] - Western blot analysis of lysates from HeLa cells, using PPP2R2A Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Western Blot: alpha Adaptin Antibody [NBP2-92798] - Analysis of extracts of various cell lines, using alpha Adaptin at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/alpha-Adaptin-Antibody-Western-Blot-NBP2-92798-img0003.jpg "Western Blot: alpha Adaptin Antibody [NBP2-92798] - Analysis of extracts of various cell lines, using alpha Adaptin at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Western Blot: PAK1 [p Thr84] Antibody [NBP2-29511] - Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~68k PAK1 protein phosphorylated at Thr84 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.](http://images.novusbio.com/fullsize/PAK1-[p-Thr84]-Antibody-Western-Blot-NBP2-29511-img0002.jpg "Western Blot: PAK1 [p Thr84] Antibody [NBP2-29511] - Western blot of mouse hippocampal lysate showing specific immunolabeling of the ~68k PAK1 protein phosphorylated at Thr84 (control). Phosphospecificity is shown in the second lane. The blot is identical to the control except that the lysate was incubated in lambda-Ptase (800 units/1mg protein for 30min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.")
![Western Blot: FANCI [p Ser559] Antibody [NBP2-60781] - Western blot of Hela cell lysate treated with UV (~254nm) for20' showing specific immunolabeling of the ~150 kDa FANCIprotein phosphorylated at Ser559 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).](http://images.novusbio.com/fullsize/FANCI-[p-Ser559]-Antibody-Western-Blot-NBP2-60781-img0003.jpg "Western Blot: FANCI [p Ser559] Antibody [NBP2-60781] - Western blot of Hela cell lysate treated with UV (~254nm) for20' showing specific immunolabeling of the ~150 kDa FANCIprotein phosphorylated at Ser559 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).")
![Western Blot: FANCI [p Ser559] Antibody [NBP2-60781] - Western blot of HeLa cell lysates that had been treated withUV (~254 nm) for 0' or 20' showing specific immunolabeling of the~150 kDa FANCI protein phosphorylated at Ser559.](http://images.novusbio.com/fullsize/FANCI-[p-Ser559]-Antibody-Western-Blot-NBP2-60781-img0001.jpg)
![Western Blot: FANCI [p Ser556] Antibody [NBP2-60780] - Western blot of Hela cell lysate treated with UV (~254nm) for20' showing specific immunolabeling of the ~150 kDa FANCIprotein phosphorylated at Ser559 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).](http://images.novusbio.com/fullsize/FANCI-[p-Ser556]-Antibody-Western-Blot-NBP2-60780-img0004.jpg "Western Blot: FANCI [p Ser556] Antibody [NBP2-60780] - Western blot of Hela cell lysate treated with UV (~254nm) for20' showing specific immunolabeling of the ~150 kDa FANCIprotein phosphorylated at Ser559 in the first lane (-).Phosphospecificity is shown in the second lane (+) whereimmunolabeling is completely eliminated by blot treatment withlambda phosphatase (Lambda-Ptase, 1200 units for 30 min).")
![Western Blot: FANCI [p Ser556] Antibody [NBP2-60780] - Western blot of HeLa cell lysates that had been treated withUV (~254 nm) for 0' or 20' showing specific immunolabeling of the~150 kDa FANCI protein phosphorylated at Ser559.](http://images.novusbio.com/fullsize/FANCI-[p-Ser556]-Antibody-Western-Blot-NBP2-60780-img0002.jpg)
![Western Blot: PLK5 Antibody [NBP3-12602] - Lane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa(We are unsure as to the identity of these extra bands.)](http://images.novusbio.com/fullsize/PLK5-Antibody-Western-Blot-NBP3-12602-img0001.jpg "Western Blot: PLK5 Antibody [NBP3-12602] - Lane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:36 kDaObserved band size:36 kDa(We are unsure as to the identity of these extra bands.)")
![Immunoprecipitation: PLK5 Antibody [NBP3-12602] - Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-PLK5 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PLK5 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 36 kDaObserved band size :36 kDa](http://images.novusbio.com/fullsize/PLK5-Antibody-Immunoprecipitation-NBP3-12602-img0002.jpg)
![Western Blot: Tau [p Ser199] Antibody (JE46-95) [NBP3-32803] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32803) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Human brain tissue lysate, the membrane treated with pp for 1 hour Lane 5: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lane 6: Rat brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 35-70 kDa Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32803) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32803_rabbit-tau-p-ser199-mab-je46-95-288202412461637.jpg "Western Blot: Tau [p Ser199] Antibody (JE46-95) [NBP3-32803] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32803) at 1/1,000 dilution. Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: Human brain tissue lysate, the membrane treated with pp for 1 hour Lane 5: Mouse brain tissue lysate, the membrane treated with pp for 1 hour Lane 6: Rat brain tissue lysate, the membrane treated with pp for 1 hour Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 35-70 kDa Exposure time: 3 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32803) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: Tau [p Ser356] Antibody (JE46-86) [NBP3-32807] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32807) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-250 kDa Exposure time: 3 minutes 19 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32807) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32807_rabbit-tau-p-ser356-mab-je46-86-28820241246167.jpg "Western Blot: Tau [p Ser356] Antibody (JE46-86) [NBP3-32807] - Western blot analysis of Tau on different lysates with Rabbit anti-Tau antibody (NBP3-32807) at 1/1,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 79 kDa Observed band size: 50-250 kDa Exposure time: 3 minutes 19 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32807) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")