| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92178-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77179-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74910 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37736-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38759-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-24559-25ul | |||
| Azide and BSA Free | NBP3-43869 | |||
![Immunocytochemistry/Immunofluorescence: Neurokinin B Antibody [NBP1-92178] - Staining of human cell line U-2 OS shows localization to vesicles.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92178-img0004.jpg "Immunocytochemistry/Immunofluorescence: Neurokinin B Antibody [NBP1-92178] - Staining of human cell line U-2 OS shows localization to vesicles.")
![Immunohistochemistry-Paraffin: Neurokinin B Antibody [NBP1-92178] - Staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/Neurokinin-B-Antibody-Immunohistochemistry-Paraffin-NBP1-92178-img0002.jpg)
![Immunohistochemistry-Paraffin: Neurokinin B Antibody [NBP1-92178] -Staining of human Placenta shows weak cytoplasmic positivity in syncytiotrophoblast.](http://images.novusbio.com/fullsize/antibody/nbp1-92178_rabbit-polyclonal-neurokinin-b-antibody-immunohistochemistry-paraffin-22120258449..png)
![Immunohistochemistry-Paraffin: Neurokinin B Antibody [NBP1-92178] -Staining of human Liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp1-92178_rabbit-polyclonal-neurokinin-b-antibody-immunohistochemistry-paraffin-22120258539..png)
![Immunocytochemistry/Immunofluorescence: Neurokinin B Antibody [NBP1-92178] -Staining of Mouse brain shows moderate positivity in cells in molecular layer in the cerebellum.](http://images.novusbio.com/fullsize/antibody/nbp1-92178_rabbit-polyclonal-neurokinin-b-antibody-immunocytochemistry-immunofluorescence-22120258647..png)
![Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-77179] - Analysis in EL4 cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 ug/ml.Red: ZEB2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202419333477.gif)
![Western Blot: ZEB2 Antibody [NBP1-77179] - Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, and then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0012.jpg)
![Western Blot: ZEB2 Antibody [NBP1-77179] - The protein levels of EMT-TFs were compared between carcinoma cell lines and non- or less-malignant tumor cell lines by using Western-blot analysis. The soft agar colony transformation or migration assay was referred to distinguish malignant degree of the experimental cancer cell lines (data not shown). Malignant degree: prostate cancer cell lines, P69 < M12; breast cancer cell lines, MCF7 < MD-MBA-231; lung cancer cell lines, A549 < H1299; liver cancer cell lines, HepG2 < SMMC7721. 'kD' (kilo-dalton) means the molecular weight of protein. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0013.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415392525.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415334920.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415395947.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-31020241535644.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Functional domains of Fbxo45 for ubiquitination of Zeb2A. Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, & then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. B. Bacterially expressed GST or GST–Fbxo45, -Fbxo45 delta F-box, -Fbxo45 delta mid, or -Fbxo45 delta SPRY fusion proteins & Glutathione-Sepharose beads were incubated with lysates of HeLa cells transfected with Zeb2, Snai1 or Snai2, respectively. The proteins associated with GST–tagged Fbxo45 forms, bound on the Glutathione-Sepharose beads were washed five times with the RIPA buffer before immunoblotting. C. Lysates from 293T cells transfected Flag-Zeb2 with HA-Fbxo45, -Fbxo45 delta mid, -Fbxo45 delta F-box or -Fbxo45 delta SPRY were immunoprecipitated with anti-Flag or anti-HA antibodies, & immunoprecipitates were resolved by SDS-PAGE for Western-blot analysis. D. U2OS cells transfected with Flag-Zeb2 & HA-Fbxo45, -Fbxo45 delta F-box or -Fbxo45 delta SPRY were stained using the primary antibodies of anti-Flag M2 or anti-HA, & the second antibodies of Alexa Fluor 568 anti-mouse or Alexa Fluor 488 anti-Rabbit, respectively. Scale: 25μm. E-I. Zeb2 ubiquitination assays by using IP expriments under different conditions: Flag-Zeb2 with or without HA-Ub (E); Flag-Zeb2 with HA-Ub WT, K48R or K63R (F); Flag-Zeb2 & HA-Ub K48-only with or without myc-Fbox45 (G); Flag-Zeb2 & HA-Ub K48-only with siRNA control, siRNAs for Fbxo45 or siRNAs for Pam (H); Flag-Zeb2 & HA-Ub with HA-Fbxo45, -Fbxo45 delta F-box or -Fbxo45 delta SPRY (I). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202416163752.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 is a direct target of miR-27a* that mediates EMT processesA, C. The expression levels of miR-27a* (A) & Fbxo45 mRNA (C) in P69 & M12 were shown according to the data of miRNA transcriptome sequencing & DGE sequencing, respectively [46]. TPM means transcripts per million. B. Real-time PCR in delta delta Ct methods were used to confirm the expression levels of miR-27a* in P69 & M12. One representative experiment out of three was shown. D. Fbxo45 mRNA levels in stable cell lines miR-27a*-overexpressing P69 & miR-27a*-silencing M12 (zipmiR-27a*). P69 or M12 stably infected with the same lentiviral empty vector was used as a control (ctrl). E. Fbxo45 3′-UTR wide-type or mutant form at the position where is complementary to the 5′ seed region of miR-27a* was subcloned into psiCheck2 vector. 293T cells were transfected with psiCheck2 containing WT or mutant Fbxo45 3′-UTR & miR-27a* or non-specific control vector. The Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. Data are the mean±S.E.M. of three independent experiments. F-G. Immunoblot for Fbxo45, core EMT-TFs (Zeb1, Zeb2, Twist1) & their associated EMT markers (Fibronectin, N-cadherin, Laminin C2, Vimentin & E-cadherin) in stable cell lines P69-miR-27a* or M12-zipmiR-27a* (F); Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20μM of MG132 for 4 h before harvested (G). GAPDH is for a loading control. H. Immunblot for Fbxo45, N-cadherin, E-cadherin & five core EMT-TFs in stable cell lines PC3-ctrl, PC3-miR-27a* & PC3-zipmiR-27a*. alpha -tubulin is for a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202416175234.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 is a direct target of miR-27a* that mediates EMT processesA, C. The expression levels of miR-27a* (A) & Fbxo45 mRNA (C) in P69 & M12 were shown according to the data of miRNA transcriptome sequencing & DGE sequencing, respectively [46]. TPM means transcripts per million. B. Real-time PCR in delta delta Ct methods were used to confirm the expression levels of miR-27a* in P69 & M12. One representative experiment out of three was shown. D. Fbxo45 mRNA levels in stable cell lines miR-27a*-overexpressing P69 & miR-27a*-silencing M12 (zipmiR-27a*). P69 or M12 stably infected with the same lentiviral empty vector was used as a control (ctrl). E. Fbxo45 3′-UTR wide-type or mutant form at the position where is complementary to the 5′ seed region of miR-27a* was subcloned into psiCheck2 vector. 293T cells were transfected with psiCheck2 containing WT or mutant Fbxo45 3′-UTR & miR-27a* or non-specific control vector. The Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. Data are the mean±S.E.M. of three independent experiments. F-G. Immunoblot for Fbxo45, core EMT-TFs (Zeb1, Zeb2, Twist1) & their associated EMT markers (Fibronectin, N-cadherin, Laminin C2, Vimentin & E-cadherin) in stable cell lines P69-miR-27a* or M12-zipmiR-27a* (F); Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20μM of MG132 for 4 h before harvested (G). GAPDH is for a loading control. H. Immunblot for Fbxo45, N-cadherin, E-cadherin & five core EMT-TFs in stable cell lines PC3-ctrl, PC3-miR-27a* & PC3-zipmiR-27a*. alpha -tubulin is for a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-31020241616011.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunocytochemistry of ZEB2 in Jurkat cells with ZEB2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202420285893.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202420272496.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2A.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Western-Blot-NBP2-45602-img0008.jpg "Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDKN2A.")
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HepG2 cells.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0003.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Carcinoma of Human liver tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0007.jpg)
![Western Blot: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: Human; HeLa: Human; HT29: Human; A549: Human; COS7: monkey; Jurkat: human; MDCK: Canine; PC12: Rat; MCF7: human)](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Western-Blot-NBP2-45602-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY CDKN2A.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of HeLa cells.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunofluorescence-NBP2-45602-img0002.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0004.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Adenocarcinoma of Human endometrium tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0005.jpg)
![Immunohistochemistry: p16INK4a/CDKN2A Antibody (OTI4C11) [NBP2-45602] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/p16INK4a-CDKN2A-Antibody-OTI4C11-Immunohistochemistry-NBP2-45602-img0006.jpg)
![Western Blot: ZEB1 Antibody (3G6) [NBP2-46398] - Cross-reactivity test with ZEB2: Analysis of HEK293T cells were transfected with plasmids overexpressing either ZEB2 (left) or ZEB1 (right).](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Western-Blot-NBP2-46398-img0007.jpg "Western Blot: ZEB1 Antibody (3G6) [NBP2-46398] - Cross-reactivity test with ZEB2: Analysis of HEK293T cells were transfected with plasmids overexpressing either ZEB2 (left) or ZEB1 (right).")
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0006.jpg)
![Western Blot: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Western-Blot-NBP2-46398-img0008.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0001.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0002.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0003.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0004.jpg)
![Immunohistochemistry: ZEB1 Antibody (3G6) [NBP2-46398] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ZEB1-Antibody-3G6-Immunohistochemistry-NBP2-46398-img0005.jpg)
![Western Blot: Brg1 Antibody (4N9C4) [NBP3-15773] - Analysis of lysates from Rat brain using BRG1/SMARCA4 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-15773_rabbit-brg1-mab-4n9c4-western-blot-612025113812..png "Western Blot: Brg1 Antibody (4N9C4) [NBP3-15773] - Analysis of lysates from Rat brain using BRG1/SMARCA4 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.")
![Western Blot: Brg1 Antibody (4N9C4) [NBP3-15773] - Analysis of lysates from Mouse brain using BRG1/SMARCA4 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-15773_rabbit-brg1-mab-4n9c4-western-blot-612025113921..png)
![Immunohistochemistry-Paraffin: Brg1 Antibody (4N9C4) [NBP3-15773] - Analysis of paraffin-embedded Human cervix cancer using BRG1/SMARCA4 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15773_rabbit-brg1-mab-4n9c4-immunohistochemistry-paraffin-612025114050..png)
![Immunohistochemistry-Paraffin: Brg1 Antibody (4N9C4) [NBP3-15773] -Analysis of paraffin-embedded Human colon carcinoma using BRG1/SMARCA4 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15773_rabbit-brg1-mab-4n9c4-immunohistochemistry-paraffin-612025114146..png)
![Immunohistochemistry: Brg1 Antibody (4N9C4) [Brg1] - Immunohistochemistry analysis of paraffin-embedded Human colon using Brg1 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15773_rabbit-brg1-mab-4n9c4-52202517304945.jpg)
![Western Blot: Brg1 Antibody (4N9C4) [Brg1] - Western blot analysis of lysates from HeLa cells using Brg1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15773_rabbit-brg1-mab-4n9c4-5220251735276.jpg)
![Immunohistochemistry: Brg1 Antibody (4N9C4) [Brg1] - Immunohistochemistry analysis of paraffin-embedded Human endometrium cancer using Brg1 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15773_rabbit-brg1-mab-4n9c4-5220251733515.jpg)
![Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (4N9C4) [Brg1] - Confocal imaging of NIH-3T3 cells using Brg1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15773_rabbit-brg1-mab-4n9c4-52202517333818.jpg)
![Immunohistochemistry: Brg1 Antibody (4N9C4) [Brg1] - Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma using Brg1 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15773_rabbit-brg1-mab-4n9c4-52202517352751.jpg)
![Immunohistochemistry: Brg1 Antibody (4N9C4) [Brg1] - Immunohistochemistry analysis of paraffin-embedded Human placenta using Brg1 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15773_rabbit-brg1-mab-4n9c4-52202517304934.jpg)
![Western Blot: Ankyrin Brain Antibody [NBP2-33863] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Western-Blot-NBP2-33863-img0003.jpg "Western Blot: Ankyrin Brain Antibody [NBP2-33863] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue")
![Immunocytochemistry/Immunofluorescence: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cell line RH-30 shows localization to plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-33863-img0008.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0012.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0004.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Analysis in human cerebral cortex and skin tissues using NBP2-33863 antibody. Corresponding ANK2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0009.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0010.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0011.jpg)
![Immunohistochemistry-Paraffin: Ankyrin Brain Antibody [NBP2-33863] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/Ankyrin-Brain-Antibody-Immunohistochemistry-Paraffin-NBP2-33863-img0013.jpg)
![Western Blot: Regucalcin Antibody [NBP1-80850] - Analysis using Anti-RGN antibody NBP1-80850 (A) shows similar pattern to independent antibody NBP1-80849 (B).](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Western-Blot-NBP1-80850-img0021.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Regucalcin Antibody [NBP1-80850] - Analysis using Anti-RGN antibody NBP1-80850 (A) shows similar pattern to independent antibody NBP1-80849 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Regucalcin Antibody [NBP1-80850] - Staining of human cell line Hep G2 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80850-img0008.jpg)
![Immunohistochemistry: Regucalcin Antibody [NBP1-80850] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/nbp1-80850_rabbit-polyclonal-regucalcin-antibody-13520231550351.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: Regucalcin Antibody [NBP1-80850] - Staining of human adrenal gland, kidney, liver and tonsil using Anti-RGN antibody NBP1-80850 (A) shows similar protein distribution across tissues to independent antibody NBP1-80849 (B).](http://images.novusbio.com/fullsize/nbp1-80850_rabbit-polyclonal-regucalcin-antibody-13520231550350.jpg)
![Immunohistochemistry: Regucalcin Antibody [NBP1-80850] - Staining of human adrenal gland shows strong positivity in glandular cells.](http://images.novusbio.com/fullsize/nbp1-80850_rabbit-polyclonal-regucalcin-antibody-13520231550354.jpg)
![Immunohistochemistry: Regucalcin Antibody [NBP1-80850] - Staining of human liver shows strong positivity in hepatocytes.](http://images.novusbio.com/fullsize/nbp1-80850_rabbit-polyclonal-regucalcin-antibody-13520231550355.jpg)
![Immunohistochemistry: Regucalcin Antibody [NBP1-80850] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/nbp1-80850_rabbit-polyclonal-regucalcin-antibody-13520231550352.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: Regucalcin Antibody [NBP1-80850] - Immunohistochemistry analysis in human adrenal gland and tonsil tissues using NBP1-80850 antibody. Corresponding RGN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/nbp1-80850_rabbit-polyclonal-regucalcin-antibody-13520231550353.jpg)
![Western Blot: NDUFA8 Antibody [NBP1-82661] - Analysis using Anti-NDUFA8 antibody NBP1-82661 (A) shows similar pattern to independent antibody NBP1-82662 (B).](http://images.novusbio.com/fullsize/NDUFA8-Antibody-Western-Blot-NBP1-82661-img0007.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NDUFA8 Antibody [NBP1-82661] - Analysis using Anti-NDUFA8 antibody NBP1-82661 (A) shows similar pattern to independent antibody NBP1-82662 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NDUFA8 Antibody [NBP1-82661] - Staining of human liver.](http://images.novusbio.com/fullsize/NDUFA8-Antibody-Immunohistochemistry-Paraffin-NBP1-82661-img0010.jpg)
![Western Blot: NDUFA8 Antibody [NBP1-82661] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).](http://images.novusbio.com/fullsize/NDUFA8-Antibody-Western-Blot-NBP1-82661-img0005.jpg)
![Immunohistochemistry-Paraffin: NDUFA8 Antibody [NBP1-82661] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NDUFA8-Antibody-Immunohistochemistry-Paraffin-NBP1-82661-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: NDUFA8 Antibody [NBP1-82661] - Staining of human cerebral cortex, colon, kidney and liver using Anti-NDUFA8 antibody NBP1-82661 (A) shows similar protein distribution across tissues to independent antibody NBP1-82662 (B).](http://images.novusbio.com/fullsize/NDUFA8-Antibody-Immunohistochemistry-Paraffin-NBP1-82661-img0006.jpg)
![Immunohistochemistry-Paraffin: NDUFA8 Antibody [NBP1-82661] - Staining of human kidney.](http://images.novusbio.com/fullsize/NDUFA8-Antibody-Immunohistochemistry-Paraffin-NBP1-82661-img0008.jpg)
![Immunohistochemistry-Paraffin: NDUFA8 Antibody [NBP1-82661] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/NDUFA8-Antibody-Immunohistochemistry-Paraffin-NBP1-82661-img0009.jpg)
![Immunohistochemistry-Paraffin: NDUFA8 Antibody [NBP1-82661] - Staining of human colon.](http://images.novusbio.com/fullsize/NDUFA8-Antibody-Immunohistochemistry-Paraffin-NBP1-82661-img0011.jpg)
![Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP2-38759] - Analysis in human fallopian tube and skeletal muscle tissues. Corresponding ASRGL1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ASRGL1-Antibody-Immunohistochemistry-Paraffin-NBP2-38759-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP2-38759] - Analysis in human fallopian tube and skeletal muscle tissues. Corresponding ASRGL1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP2-38759] - Staining of human cervix, uterine, endometrium, fallopian tube and skeletal muscle using Anti-ASRGL1 antibody NBP2-38759 (A) shows similar protein distribution across tissues to independent antibody NBP1-89133 (B).](http://images.novusbio.com/fullsize/ASRGL1-Antibody-Immunohistochemistry-Paraffin-NBP2-38759-img0008.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ASRGL1 Antibody [NBP2-38759] - Analysis in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ASRGL1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/ASRGL1-Antibody-Western-Blot-NBP2-38759-img0007.jpg)
![Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP2-38759] - Staining of human endometrium shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ASRGL1-Antibody-Immunohistochemistry-Paraffin-NBP2-38759-img0012.jpg)
![Western Blot: ASRGL1 Antibody [NBP2-38759] - Analysis in control (vector only transfected HEK293T lysate) and ASRGL1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/ASRGL1-Antibody-Western-Blot-NBP2-38759-img0006.jpg)
![Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP2-38759] - Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ASRGL1-Antibody-Immunohistochemistry-Paraffin-NBP2-38759-img0010.jpg)
![Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP2-38759] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ASRGL1-Antibody-Immunohistochemistry-Paraffin-NBP2-38759-img0011.jpg)
![Immunohistochemistry-Paraffin: ASRGL1 Antibody [NBP2-38759] - Staining of human cervix, uterine shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ASRGL1-Antibody-Immunohistochemistry-Paraffin-NBP2-38759-img0013.jpg)
![Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249422625.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249374983.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249374923.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249422673.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249393552.jpg)
![Western Blot: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-212024941465.jpg)
![Immunohistochemistry-Paraffin: ZEB1 Antibody (HL2245) - Azide and BSA Free [NBP3-25767] - ZEB1 antibody [HL2245] detects ZEB1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. ZEB1 stained by ZEB1 antibody [HL2245] (NBP3-25767) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25767_rabbit-zeb1-mab-hl2245-azide-and-bsa-free-2120249415991.jpg)