| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42189-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87694-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81028-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54665-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54590-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-46768-0.1mg | |||
![Western Blot: SHANK3 Antibody (S367-51) [NBP2-42189] - Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (NBP2-42189). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (NBP2-42189) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S367-51-Western-Blot-NBP2-42189-img0003.jpg "Western Blot: SHANK3 Antibody (S367-51) [NBP2-42189] - Western Blot analysis of Monkey COS cells transfected with HA-tagged Shank1 showing detection of ~190 kDa SHANK1/SHANK3 protein using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (NBP2-42189). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with HA-tagged Shank1. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (NBP2-42189) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~190 kDa.")
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S367-51) [NBP2-42189] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody, Clone S367-51 (NBP2-42189). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody (NBP2-42189) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1/SHANK3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S367-51-Immunocytochemistry-Immunofluorescence-NBP2-42189-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody (S367-51) [NBP2-42189] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK1/SHANK3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm, Nucleus. Magnification: 60X.](http://images.novusbio.com/fullsize/SHANK3-Antibody-S367-51-Immunocytochemistry-Immunofluorescence-NBP2-42189-img0002.jpg)
![Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB100-1606] - Western blot showing a single band at the correct MW (47 kDa).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-Western-Blot-NB100-1606-img0003.jpg "Western Blot: Enolase 2/Neuron-specific Enolase Antibody [NB100-1606] - Western blot showing a single band at the correct MW (47 kDa).")
![Immunocytochemistry/ Immunofluorescence: CXXC1 Antibody (SR2392) [NBP3-27690] - Immunocytochemistry analysis of CXXC1 (green) in Hela using NBP3-27690, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-27690_rabbit-cxxc1-mab-sr2392-75202410133465.jpg "Immunocytochemistry/ Immunofluorescence: CXXC1 Antibody (SR2392) [NBP3-27690] - Immunocytochemistry analysis of CXXC1 (green) in Hela using NBP3-27690, and DAPI (blue)")
![Immunohistochemistry-Paraffin: CXXC1 Antibody (SR2392) [NBP3-27690] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.](http://images.novusbio.com/fullsize/nbp3-27690_rabbit-cxxc1-mab-sr2392-75202410133415.jpg)
![Western Blot: CXXC1 Antibody (SR2392) [NBP3-27690] - Western blot analysis of CGBP in C6, 3T3, Hela lysates](http://images.novusbio.com/fullsize/nbp3-27690_rabbit-cxxc1-mab-sr2392-752024120319.jpg)
![Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody [NBP1-87694] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87694-img0006.jpg "Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody [NBP1-87694] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [NBP1-87694] - Staining of human colon shows strong nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/KAT3B-p300-Antibody-Immunohistochemistry-Paraffin-NBP1-87694-img0005.jpg)
![Western Blot: SHANK3 Antibody [NBP3-13162] - Various tissue extracts (75 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Shank3 antibody (NBP3-13162) diluted at 1:1000.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Western-Blot-NBP3-13162-img0001.jpg "Western Blot: SHANK3 Antibody [NBP3-13162] - Various tissue extracts (75 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Shank3 antibody (NBP3-13162) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: SHANK3 Antibody [NBP3-13162] - SHANK3 antibody detects SHANK3 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SHANK3 protein stained by SHANK3 antibody (NBP3-13162) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13162-img0002.jpg)
![Immunohistochemistry-Frozen: SHANK3 Antibody [NBP3-13162] - SHANK3 antibody detects SHANK3 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: SHANK3 protein stained by SHANK3 antibody (NBP3-13162) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SHANK3-Antibody-Immunohistochemistry-Frozen-NBP3-13162-img0003.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81028] - Staining of human cerebellum, lymph node, prostate and testis using Anti-MeCP2 antibody NBP1-81028 (A) shows similar protein distribution across tissues to independent antibody NBP1-81027 (B).](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81028-img0012.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81028] - Staining of human cerebellum, lymph node, prostate and testis using Anti-MeCP2 antibody NBP1-81028 (A) shows similar protein distribution across tissues to independent antibody NBP1-81027 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81028] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81028-img0015.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81028] - Staining of human cerebellum shows strong nuclear positivity in cells in granular layer.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81028-img0010.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81028] - Staining of human lymph node shows strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81028-img0013.jpg)
![Immunohistochemistry-Paraffin: MeCP2 Antibody [NBP1-81028] - Staining of human prostate shows moderate to strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/MeCP2-Antibody-Immunohistochemistry-Paraffin-NBP1-81028-img0014.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-54665] - Staining in human kidney and lymph node tissues using anti-ATP1B1 antibody. Corresponding ATP1B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-54665-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-54665] - Staining in human kidney and lymph node tissues using anti-ATP1B1 antibody. Corresponding ATP1B1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP2-54665] - Analysis in human cell lines Caco-2 and SK-MEL-30 using anti-ATP1B1 antibody. Corresponding ATP1B1 RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Western-Blot-NBP2-54665-img0007.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-54665] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-54665-img0003.jpg)
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP2-54665] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP2-54665-img0004.jpg)
![Western Blot: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Western blot analysis of Jumonji/JARID2 on different lysates with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: MDA-MB-231 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: HepG2 cell lysate (20 ug/Lane) Lane 8: HUVEC cell lysate (20 ug/Lane) Lane 9: HeLa cell lysate (20 ug/Lane) Lane 10: HEK-293 cell lysate (20 ug/Lane) Lane 11: Human brain tissue lysate (40 ug/Lane) Predicted band size: 139 kDa Observed band size: 139 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32486) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-278202416285253.jpg "Western Blot: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Western blot analysis of Jumonji/JARID2 on different lysates with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (20 ug/Lane) Lane 2: SH-SY5Y cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (20 ug/Lane) Lane 4: Jurkat cell lysate (20 ug/Lane) Lane 5: MDA-MB-231 cell lysate (20 ug/Lane) Lane 6: A549 cell lysate (20 ug/Lane) Lane 7: HepG2 cell lysate (20 ug/Lane) Lane 8: HUVEC cell lysate (20 ug/Lane) Lane 9: HeLa cell lysate (20 ug/Lane) Lane 10: HEK-293 cell lysate (20 ug/Lane) Lane 11: Human brain tissue lysate (40 ug/Lane) Predicted band size: 139 kDa Observed band size: 139 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32486) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32486) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-298202414304155.jpg)
![Immunocytochemistry/ Immunofluorescence: Jumonji/JARID2 Antibody (PSH01-32) [NBP3-32486] - Immunocytochemistry analysis of NCCIT cells labeling Jumonji/JARID2 with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Jumonji/JARID2 antibody (NBP3-32486) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32486_rabbit-jumonji-jarid2-mab-psh01-32-29202410484844.jpg)
![Immunohistochemistry-Frozen: Substance P Antibody (NC1/34) [NB100-65219] - Opioid R/OPRM1 (green) and Substance P (red) were detected in frozen mouse spinal cord and DRG tissue by fluorescent multiplex IHC. Mu Opioid R/OPRM1 Antibody (NB100-1620), used at 1:100, and Substance P Antibody (NB100-65219), used at 1:50, were applied to the tissue and simultaneously incubated at 4C overnight. Anti-Rabbit Alexa Fluor(R) 488-conjugated secondary antibody and anti-Rat NL557-conjugated secondary antibody (NL013) were applied to the tissue, and simultaneously incubated at room temperature, protected from light for 1 hour. Coverslips were applied to the tissue slide along with mounting media containing DAPI (blue) and imaged using a 4X objective lens.](http://images.novusbio.com/fullsize/Substance-P-Antibody-NC1-34-Immunohistochemistry-Frozen-NB100-65219-img0001.jpg "Immunohistochemistry-Frozen: Substance P Antibody (NC1/34) [NB100-65219] - Opioid R/OPRM1 (green) and Substance P (red) were detected in frozen mouse spinal cord and DRG tissue by fluorescent multiplex IHC. Mu Opioid R/OPRM1 Antibody (NB100-1620), used at 1:100, and Substance P Antibody (NB100-65219), used at 1:50, were applied to the tissue and simultaneously incubated at 4C overnight. Anti-Rabbit Alexa Fluor(R) 488-conjugated secondary antibody and anti-Rat NL557-conjugated secondary antibody (NL013) were applied to the tissue, and simultaneously incubated at room temperature, protected from light for 1 hour. Coverslips were applied to the tissue slide along with mounting media containing DAPI (blue) and imaged using a 4X objective lens.")
![Western Blot: MeCP2 [p Ser80] Antibody [NBP3-23406] - Western blot of adult mouse brain tissue lysate. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (Ser-80) (lanes 1 & 2) or anti-MeCP2 (C-terminus) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23406_rabbit-mecp2-p-ser80-pab-19920231252361.jpg "Western Blot: MeCP2 [p Ser80] Antibody [NBP3-23406] - Western blot of adult mouse brain tissue lysate. The blot lanes were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4) then probed with rabbit polyclonals anti-MeCP2 (Ser-80) (lanes 1 & 2) or anti-MeCP2 (C-terminus) (lanes 3 & 4).")
![Immunocytochemistry/Immunofluorescence: MeCP2 [p Ser80] Antibody [NBP3-23406] - Immunocytochemical labeling of MeCP2 phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with MeCP2 (Ser-80) rabbit polyclonal antibody (NBP3-23406) in the absence (left) or presence (right) of blocking peptide. The antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23406_rabbit-mecp2-p-ser80-pab-19920231915413.jpg)
![Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (SPM347) [NBP2-53158] - Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (SPM347).](http://images.novusbio.com/fullsize/Enolase-2-Neuron-specific-Enolase-Antibody-SPM347-Immunohistochemistry-Paraffin-NBP2-53158-img0001.jpg "Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (SPM347) [NBP2-53158] - Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (SPM347).")