| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12915-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37281-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42200-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61746-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52520-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12913-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-42190-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97747-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09006-0.025mg | |||
![Western Blot: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Analysis of extracts of various cell lines, using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-8T4D3-Western-Blot-NBP3-15454-img0002.jpg "Western Blot: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Analysis of extracts of various cell lines, using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Immunofluorescence analysis of Hep G2 cells using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-8T4D3-Immunocytochemistry-Immunofluorescence-NBP3-15454-img0001.jpg)
![Western Blot: Sonic Hedgehog/Shh Antibody (8T4D3) [NBP3-15454] - Analysis of extracts of various cell lines, using Sonic Hedgehog/Shh (Shh) Rabbit mAb (NBP3-15454) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Sonic-Hedgehog-Shh-Antibody-8T4D3-Western-Blot-NBP3-15454-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog/Shh Antibody (8T4D3) [Sonic Hedgehog/Shh] - Confocal imaging of paraffin-embedded Mouse kidney using Sonic Hedgehog/Shh Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-15454_rabbit-sonic-hedgehog-shh-mab-8t4d3-522025171899.jpg)
![Immunocytochemistry/ Immunofluorescence: Sonic Hedgehog/Shh Antibody (8T4D3) [Sonic Hedgehog/Shh] - Confocal imaging of Hep G2 cells using Sonic Hedgehog/Shh Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15454_rabbit-sonic-hedgehog-shh-mab-8t4d3-52202517192951.jpg)
![Western Blot: SHANK1 Antibody (N22/21) [NBP2-12915] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK1 protein using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (NBP2-12915). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (NBP2-12915) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/SHANK1-Antibody-N22-21-Western-Blot-NBP2-12915-img0004.jpg "Western Blot: SHANK1 Antibody (N22/21) [NBP2-12915] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK1 protein using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (NBP2-12915). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (NBP2-12915) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: SHANK1 Antibody (N22/21) [NBP2-12915] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (NBP2-12915). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (NBP2-12915) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/SHANK1-Antibody-N22-21-Immunocytochemistry-Immunofluorescence-NBP2-12915-img0006.jpg)
![Immunohistochemistry: SHANK1 Antibody (N22/21) [NBP2-12915] - Immunohistochemistry analysis using Mouse Anti-SHANK1 Monoclonal Antibody, Clone S22-21 (NBP2-12915). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-SHANK1 Monoclonal Antibody (NBP2-12915) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining (upper layer aggregations of staining).](http://images.novusbio.com/fullsize/SHANK1-Antibody-N22-21-Immunohistochemistry-NBP2-12915-img0005.jpg)
![Western Blot: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Western blot analysis using GABBR2 mAb against HEK293 (1) and GABBR2 (AA: 319-483)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Western-Blot-NBP2-37281-img0003.jpg "Western Blot: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Western blot analysis using GABBR2 mAb against HEK293 (1) and GABBR2 (AA: 319-483)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Immunofluorescence analysis of Hela cells using GABBR2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Immunocytochemistry-Immunofluorescence-NBP2-37281-img0004.jpg)
![Immunohistochemistry: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Immunohistochemical analysis of paraffin-embedded cerebellum tissues using GABBR2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Immunohistochemistry-NBP2-37281-img0005.jpg)
![Western Blot: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Western blot analysis using GABBR2 mAb against human GABBR2 (AA: 319-483) recombinant protein. (Expected MW is 44.9 kDa)](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-Western-Blot-NBP2-37281-img0002.jpg)
![ELISA: GABA-B R2 Antibody (5G7F7) [NBP2-37281] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/GABA-B-R2-Antibody-5G7F7-ELISA-NBP2-37281-img0001.jpg)
![Western Blot: Neuroligin 3/NLGN3 Antibody (S110-29) [NBP2-42200] - Western Blot analysis of Mouse Brain Membrane showing detection of ~110 kDa Neuroligin 3/NLGN3 protein using Mouse Anti-Neuroligin 3/NLGN3 Monoclonal Antibody, Clone S110-29 (NBP2-42200). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 3/NLGN3 Monoclonal Antibody (NBP2-42200) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa.](http://images.novusbio.com/fullsize/Neuroligin-3-NLGN3-Antibody-S110-29-Western-Blot-NBP2-42200-img0003.jpg "Western Blot: Neuroligin 3/NLGN3 Antibody (S110-29) [NBP2-42200] - Western Blot analysis of Mouse Brain Membrane showing detection of ~110 kDa Neuroligin 3/NLGN3 protein using Mouse Anti-Neuroligin 3/NLGN3 Monoclonal Antibody, Clone S110-29 (NBP2-42200). Lane 1: Molecular Weight Ladder. Lane 2: Mouse Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Neuroligin 3/NLGN3 Monoclonal Antibody (NBP2-42200) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa.")
![Immunocytochemistry/Immunofluorescence: Neuroligin 3/NLGN3 Antibody (S110-29) [NBP2-42200] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 3/NLGN3 Monoclonal Antibody, Clone S110-29 (NBP2-42200). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Neuroligin 3/NLGN3 Monoclonal Antibody (NBP2-42200) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Neuroligin 3/NLGN3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Neuroligin-3-NLGN3-Antibody-S110-29-Immunocytochemistry-Immunofluorescence-NBP2-42200-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Neuroligin 3/NLGN3 Antibody (S110-29) [NBP2-42200] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Neuroligin 3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction, Synapse . Magnification: 60X.](http://images.novusbio.com/fullsize/Neuroligin-3-NLGN3-Antibody-S110-29-Immunocytochemistry-Immunofluorescence-NBP2-42200-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Neuroligin 3/NLGN3 Antibody (S110-29) [NBP2-42200] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Neuroligin 3/NLGN3 Monoclonal Antibody, Clone S110-29 (NBP2-42200). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Neuroligin 3/NLGN3 Monoclonal Antibody (NBP2-42200) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction, Synapse . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Neuroligin 3/NLGN3 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Neuroligin-3-NLGN3-Antibody-S110-29-Immunocytochemistry-Immunofluorescence-NBP2-42200-img0005.jpg)
![Western Blot: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis using GRIN2B mAb against human GRIN2B (AA: extra 27-163) recombinant protein. (Expected MW is 41 kDa)](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-Western-Blot-NBP2-61746-img0005.jpg "Western Blot: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis using GRIN2B mAb against human GRIN2B (AA: extra 27-163) recombinant protein. (Expected MW is 41 kDa)")
![Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis of SK-N-SH cells using GRIN2B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-Immunocytochemistry-Immunofluorescence-NBP2-61746-img0004.jpg)
![Flow Cytometry: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis of SK-N-SH cells using GRIN2B mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-Flow-Cytometry-NBP2-61746-img0002.jpg)
![Western Blot: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis using GRIN2B mAb against HEK293 (1) and GRIN2B (AA: extra 27-163)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-Western-Blot-NBP2-61746-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Analysis of Hela cells using GRIN2B mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-Immunocytochemistry-Immunofluorescence-NBP2-61746-img0003.jpg)
![ELISA: NMDAR2B Antibody (6E9A8) [NBP2-61746] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-6E9A8-ELISA-NBP2-61746-img0001.jpg)
![Western Blot: MeCP2 Antibody (5H12) [NBP2-60787] - Western blot of mouse whole brain lysate showing specificimmunolabeling of the MeCP2 protein at ~75 kDa.](http://images.novusbio.com/fullsize/MeCP2-Antibody-5H12-Western-Blot-NBP2-60787-img0001.jpg "Western Blot: MeCP2 Antibody (5H12) [NBP2-60787] - Western blot of mouse whole brain lysate showing specificimmunolabeling of the MeCP2 protein at ~75 kDa.")
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787] - Immunofluorescence of a section of rat cerebellum labeled with anti-MeCP2 (1:500, red), colabeled with anti-GFAP (1:5000, green), and DAPI staining of nuclear DNA. The anti-GFAP labels the astrocytic cells and the processes of Bergmann glia in the molecular layer.](http://images.novusbio.com/fullsize/MeCP2-Antibody-5H12-Immunocytochemistry-Immunofluorescence-NBP2-60787-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787] - Rat hippocampal section showing specific nuclear MeCP2immunolabeling in red and FOX3 in green.](http://images.novusbio.com/fullsize/MeCP2-Antibody-5H12-Immunocytochemistry-Immunofluorescence-NBP2-60787-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MeCP2 Antibody (5H12) [NBP2-60787] - Immunofluorescence of a section of rat cerebellum showing specific labeling of MeCP2 (1:1000, red) in nuclei of neurons and specific labeling of GFAP (1:5000, green) in the network of astroglial cells and projections of Bergmann glia, and Hoechst staining of nuclear DNA.](http://images.novusbio.com/fullsize/MeCP2-Antibody-5H12-Immunocytochemistry-Immunofluorescence-NBP2-60787-img0003.jpg)
![Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against human NOS1 (AA: 17-153) recombinant protein. (Expected MW is 40.3 kDa)](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Western-Blot-NBP2-52520-img0003.jpg "Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against human NOS1 (AA: 17-153) recombinant protein. (Expected MW is 40.3 kDa)")
![Immunocytochemistry/Immunofluorescence: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis of HeLa cells using NOS1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Immunofluorescence-NBP2-52520-img0002.jpg)
![Flow Cytometry: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis of HeLa cells using NOS1 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Flow-Cytometry-NBP2-52520-img0001.jpg)
![Western Blot: nNOS Antibody (2E11G6) [NBP2-52520] - Analysis using NOS1 mAb against HEK293 (1) and NOS1 (AA: 17-153)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-Western-Blot-NBP2-52520-img0004.jpg)
![ELISA: nNOS Antibody (2E11G6) [NBP2-52520] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/nNOS-Antibody-2E11G6-ELISA-NBP2-52520-img0005.jpg)
![Western Blot: SHANK2 Antibody (N23b/49) [NBP2-12913] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody (NBP2-12913) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/SHANK2-Antibody-N23b-49-Western-Blot-NBP2-12913-img0003.jpg "Western Blot: SHANK2 Antibody (N23b/49) [NBP2-12913] - Western Blot analysis of Rat brain membrane lysate showing detection of SHANK2 protein using Mouse Anti-SHANK2 Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-SHANK2 Monoclonal Antibody (NBP2-12913) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 (pan) Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SHANK2 (pan) Monoclonal Antibody (NBP2-12913) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SHANK2 (pan) Antibody (D) Composite.](http://images.novusbio.com/fullsize/SHANK2-Antibody-N23b-49-Immunocytochemistry-Immunofluorescence-NBP2-12913-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SHANK2 Antibody (N23b/49) [NBP2-12913] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SHANK2 (pan) Monoclonal Antibody, Clone S23b-49 (NBP2-12913). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SHANK2 (pan) Monoclonal Antibody (NBP2-12913) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SHANK2 (pan) Antibody. (D) Composite.](http://images.novusbio.com/fullsize/SHANK2-Antibody-N23b-49-Immunocytochemistry-Immunofluorescence-NBP2-12913-img0005.jpg)
![Western Blot: Lgi1 Antibody (S283-7) [NBP2-42190] - Western Blot analysis of Rat Brain Membrane showing detection of ~60 kDa Lgi1 protein using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Load: 10 ug. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~60 kDa.](http://images.novusbio.com/fullsize/Lgi1-Antibody-S283-7-Western-Blot-NBP2-42190-img0003.jpg "Western Blot: Lgi1 Antibody (S283-7) [NBP2-42190] - Western Blot analysis of Rat Brain Membrane showing detection of ~60 kDa Lgi1 protein using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Load: 10 ug. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~60 kDa.")
![Immunocytochemistry/Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Lgi1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Lgi1-Antibody-S283-7-Immunocytochemistry-Immunofluorescence-NBP2-42190-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LGI1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Golgi Apparatus, Endoplasmic Reticulum. Magnification: 60X.](http://images.novusbio.com/fullsize/Lgi1-Antibody-S283-7-Immunocytochemistry-Immunofluorescence-NBP2-42190-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Lgi1 Monoclonal Antibody (NBP2-42190) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Junction, Golgi Apparatus, Endoplasmic Reticulum. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Lgi1 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/Lgi1-Antibody-S283-7-Immunocytochemistry-Immunofluorescence-NBP2-42190-img0005.jpg)
![Western Blot: ADNP Antibody [NBP2-97747] - Anti-ADNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/ADNP-Antibody-Western-Blot-NBP2-97747-img0003.jpg "Western Blot: ADNP Antibody [NBP2-97747] - Anti-ADNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Hela Whole Cell Lysate. Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane.")
![Immunocytochemistry/Immunofluorescence: ADNP Antibody [NBP2-97747] - Immunofluorescence staining of ADNP in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human ADNP polyclonal antibody (dilution ratio 1:300) at 4C overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG Secondary antibody (red). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-97747-img0001.jpg)
![Immunoprecipitation: ADNP Antibody [NBP2-97747] - ADNP was immunoprecipitated using: Lane A: 0.5 mg U-251MG Whole Cell Lysate. 4 ul anti-ADNP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-ADNP rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 122 kDa. Observed band size: 150 kDa.](http://images.novusbio.com/fullsize/ADNP-Antibody-Immunoprecipitation-NBP2-97747-img0004.jpg)
![Immunohistochemistry-Frozen: RBFOX3/NeuN Antibody (PD01-45) [NBP3-32644] - Immunofluorescence analysis of frozen mouse hippocampus tissue labeling RBFOX3/NeuN with Mouse anti-RBFOX3/NeuN antibody (NBP3-32644).The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32644, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32644_mouse-rbfox3-neun-mab-pd01-45-2682024159391.jpg "Immunohistochemistry-Frozen: RBFOX3/NeuN Antibody (PD01-45) [NBP3-32644] - Immunofluorescence analysis of frozen mouse hippocampus tissue labeling RBFOX3/NeuN with Mouse anti-RBFOX3/NeuN antibody (NBP3-32644).The tissues were blocked in 3% BSA for 30 minutes at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32644, green) at 1/50 dilution overnight at 4℃, washed with PBS. Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).")
![Western Blot: RBFOX3/NeuN Antibody (PD01-45) [NBP3-32644] - Western blot analysis of RBFOX3/NeuN on mouse cerebellum tissue lysates with Mouse anti-RBFOX3/NeuN antibody (NBP3-32644) at 1/1,000 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 34 kDa Observed band size: 45/50 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32644) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32644_mouse-rbfox3-neun-mab-pd01-45-27820241633121.jpg)
![Immunohistochemistry: RBFOX3/NeuN Antibody (PD01-45) [NBP3-32644] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-RBFOX3/NeuN antibody (NBP3-32644) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32644) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32644_mouse-rbfox3-neun-mab-pd01-45-298202414115415.jpg)
![Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Western-Blot-NBP3-09006-img0002.jpg "Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of F2C (NBP3-09006) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing microtubule staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09006, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09006-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Anti-Alpha Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of F2C (NBP3-09006). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP3-09006 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of axons and a few neuronal cell bodies may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunohistochemistry-Paraffin-NBP3-09006-img0001.jpg)
![Flow Cytometry: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HepG2 (A), HeLa (B) and Hek293 (C) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F2C (NBP3-09006, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Flow-Cytometry-NBP3-09006-img0003.jpg)