| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89768-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03471-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50052SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89767-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43805 | |||
| BSA Free | NBP2-46643-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83277-25ul | |||
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human cerebellum, cerebral cortex, kidney and liver using Anti-GLUL antibody NBP1-89768 (A) shows similar protein distribution across tissues to independent antibody NBP1-89767 (B).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0008.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human cerebellum, cerebral cortex, kidney and liver using Anti-GLUL antibody NBP1-89768 (A) shows similar protein distribution across tissues to independent antibody NBP1-89767 (B).")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining in human cerebral cortex and kidney tissues using NBP1-89768 antibody. Corresponding GLUL RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0012.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0009.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human kidney shows only very weak cytoplasmic positivity in a subset of cells in tubules.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0010.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human cerebellum shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0007.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89768] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89768-img0011.jpg)
![Western Blot: Glutamine Synthetase Antibody [NBP3-03471] - Western blot analysis of extracts of various cell lines, using Glutamine Synthetase antibody (NBP3-03471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Glutamine Synthetase Antibody-Western Blot-NBP3-03471-img0008.jpg "Western Blot: Glutamine Synthetase Antibody [NBP3-03471] - Western blot analysis of extracts of various cell lines, using Glutamine Synthetase antibody (NBP3-03471) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-03471] - Immunohistochemistry of paraffin-embedded rat liver using [KO Validated] Glutamine Synthetase Rabbit pAb (NBP3-03471) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP3-03471-img0006.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-03471] - Immunohistochemistry of paraffin-embedded human esophageal using [KO Validated] Glutamine Synthetase Rabbit pAb (NBP3-03471) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP3-03471-img0004.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP3-03471] - Immunohistochemistry of paraffin-embedded human liver using [KO Validated] Glutamine Synthetase Rabbit pAb (NBP3-03471) at dilution of 1:150 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP3-03471-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody - BSA Free [Glutamine Synthetase] - Immunofluorescence analysis of NIH/3T3 cells using Glutamine Synthetase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03471_rabbit-polyclonal-glutamine-synthetase-antibody-5220251628083.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutamine Synthetase Antibody - BSA Free [Glutamine Synthetase] - Immunofluorescence analysis of PC-12 cells using Glutamine Synthetase Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03471_rabbit-polyclonal-glutamine-synthetase-antibody-5220251632149.jpg)
![Western Blot: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Analysis of Beta III Tubulin on different lysates with Rabbit anti-Beta III Tubulin antibody at 1/500 dilution. Lane 1: PC-12 cell lysate, Lane 2: Hela cell lysateLane 3: NIH/3T3 cell lysate, Lane 4: SH-SY5Y cell lysate. Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa, Observed band size: 50 kDa. Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-SP06-00-Western-Blot-NBP2-67374-img0006.jpg "Western Blot: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Analysis of Beta III Tubulin on different lysates with Rabbit anti-Beta III Tubulin antibody at 1/500 dilution. Lane 1: PC-12 cell lysate, Lane 2: Hela cell lysateLane 3: NIH/3T3 cell lysate, Lane 4: SH-SY5Y cell lysate. Lysates/proteins at 10 ug/Lane. Predicted band size: 50 kDa, Observed band size: 50 kDa. Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Staining Tubulin beta-III in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-SP06-00-Immunocytochemistry-Immunofluorescence-NBP2-67374-img0002.jpg)
![Immunohistochemistry-Paraffin: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Analysis of paraffin-embedded mouse brain tissue using anti-Tubulin beta-III antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-SP06-00-Immunohistochemistry-Paraffin-NBP2-67374-img0003.jpg)
![Flow Cytometry: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Flow cytometric analysis of beta-III Tubulin was done on N2A cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-SP06-00-Flow-Cytometry-NBP2-67374-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Staining Tubulin beta-III in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/beta-III-Tubulin-Antibody-SP06-00-Immunocytochemistry-Immunofluorescence-NBP2-67374-img0001.jpg)
![Western Blot: GAP-43 Antibody (5E8) [NBP2-50052] - Analysis of different tissue and cell lysates using mouse mAb to GAP43, NBP2-50052, dilution 1:2,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. Single band at 43 kDa mark correspond to GAP43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.](http://images.novusbio.com/fullsize/GAP-43-Antibody-5E8-Western-Blot-NBP2-50052-img0005.jpg "Western Blot: GAP-43 Antibody (5E8) [NBP2-50052] - Analysis of different tissue and cell lysates using mouse mAb to GAP43, NBP2-50052, dilution 1:2,000, in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. Single band at 43 kDa mark correspond to GAP43 protein. The protein is expressed in rodent and human neurons and neuronal derived cells but not in C6 cells which are of glial origin.")
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody (5E8) [NBP2-50052] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to GAP43, NBP2-50052, dilution 1:1,000, in red, and costained with chicken pAb to GFAP, dilution 1:2,000, in green. The blue is DAPI staining of nuclear DNA. GAP43 antibody labels protein expressed in the axonal membrane of the neuronal cells, and GFAP antibody stains astroglia.](http://images.novusbio.com/fullsize/GAP-43-Antibody-5E8-Immunocytochemistry-Immunofluorescence-NBP2-50052-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GAP-43 Antibody (5E8) [NBP2-50052] - Mixed rat neuronal cultures stained with NBP2-50052 (green). The GAP43 antibody stains the plasma membrane of neurons and is particularly concentrated in dendrites.](http://images.novusbio.com/fullsize/GAP-43-Antibody-5E8-Immunocytochemistry-Immunofluorescence-NBP2-50052-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NBP1-89767] - Staining of human cell line U-2 OS shows localization to cytosol & mitochondria.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89767-img0008.jpg "Immunocytochemistry/Immunofluorescence: Glutamine Synthetase Antibody [NBP1-89767] - Staining of human cell line U-2 OS shows localization to cytosol & mitochondria.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89767] - Staining of human cerebellum, cerebral cortex, kidney and liver using Anti-GLUL antibody NBP1-89767 (A) shows similar protein distribution across tissues to independent antibody NBP1-89768 (B).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89767-img0011.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89767] - Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89767-img0012.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89767] - Staining of human kidney shows only very weak cytoplasmic positivity in a subset of cells in tubules.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89767-img0013.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89767] - Staining of human cerebellum shows strong cytoplasmic positivity in glial cells.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89767-img0010.jpg)
![Immunohistochemistry-Paraffin: Glutamine Synthetase Antibody [NBP1-89767] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-Immunohistochemistry-Paraffin-NBP1-89767-img0014.jpg)
![Western Blot: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Western-Blot-NBP2-46643-img0006.jpg "Western Blot: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue")
![Immunohistochemistry: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Staining of human pancreas shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Immunohistochemistry-NBP2-46643-img0004.jpg)
![Western Blot: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Western-Blot-NBP2-46643-img0005.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Staining of rat cerebral cortex shows immunoreactivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Immunohistochemistry-NBP2-46643-img0001.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Staining of mouse hippocampal formation shows positivity in a subset of astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Immunohistochemistry-NBP2-46643-img0002.jpg)
![Immunohistochemistry: Glutamine Synthetase Antibody (2999) [NBP2-46643] - Staining of human cerebral cortex shows strong immunoreactivity in astrocytes.](http://images.novusbio.com/fullsize/Glutamine-Synthetase-Antibody-2999-Immunohistochemistry-NBP2-46643-img0003.jpg)
![Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82483] - Analysis in human cerebral cortex and liver tissues. Corresponding OMgp RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82483-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82483] - Analysis in human cerebral cortex and liver tissues. Corresponding OMgp RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82483] - Staining of human caudate nucleus, cerebral cortex, kidney and liver using Anti-OMgp antibody NBP1-82483 (A) shows similar protein distribution across tissues to independent antibody NBP1-82484 (B).](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82483-img0019.jpg)
![Western Blot: OMgp Antibody [NBP1-82483] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/OMgp-Antibody-Western-Blot-NBP1-82483-img0007.jpg)
![Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82483] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82483-img0021.jpg)
![Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82483] - Staining of human kidney.](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82483-img0014.jpg)
![Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82483] - Staining of human caudate nucleus shows moderate to strong positivity in neuronal processes.](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82483-img0016.jpg)
![Immunohistochemistry-Paraffin: OMgp Antibody [NBP1-82483] - Staining of human cerebral cortex shows moderate positivity in neuropil, as well as staining in glial cells.](http://images.novusbio.com/fullsize/OMgp-Antibody-Immunohistochemistry-Paraffin-NBP1-82483-img0018.jpg)
![Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP2-17201] - Rat fore brain. MAG antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-Paraffin-NBP2-17201-img0006.jpg "Immunohistochemistry-Paraffin: MAG/Siglec-4a Antibody [NBP2-17201] - Rat fore brain. MAG antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.")
![Immunohistochemistry-Frozen: MAG/Siglec-4a Antibody [NBP2-17201] - Frozen-sectioned adult mouse cerebellum. Green: MAG protein stained by MAG antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [11710] (NBP2-43559) diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/MAG-Siglec-4a-Antibody-Immunohistochemistry-Frozen-NBP2-17201-img0005.jpg)
![Immunohistochemistry-Paraffin: LEDGF Antibody [NB110-40560] - Detection of human LEDGF/p75 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-LEDGF/p75 antibody (NB110-40560). Secondary: HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunohistochemistry-Paraffin-NB110-40560-img0005.jpg "Immunohistochemistry-Paraffin: LEDGF Antibody [NB110-40560] - Detection of human LEDGF/p75 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-LEDGF/p75 antibody (NB110-40560). Secondary: HRP-conjugated goat anti-rabbit IgG.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: LEDGF Antibody [NB110-40560] - Detection of human LEDGF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LEDGF antibody NB110-40560 used for IP at 3 ug per reaction. LEDGF was also immunoprecipitated by rabbit anti-LEDGF antibody NB110-40561. For blotting immunoprecipitated LEDGF, NB110-40560 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Immunoprecipitation-NB110-40560-img0006.jpg)
![Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-288202414233110.jpg "Western Blot: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Western blot analysis of alpha Tubulin on different lysates with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: Daudi cell lysate Lane 3: Jurkat cell lysate Lane 4: A431 cell lysate Lane 5: K-562 cell lysate Lane 6: 293T cell lysate Lane 7: NIH/3T3 cell lysate Lane 8: PC-12 cell lysate Lane 9: Rat brain tissue lysate Lane 10: Mouse brain tissue lysate Lane 11: Mouse spleen tissue lysate Cell lysates/proteins at 20 ug/Lane. Ttissue lysates/proteins at 40 ug/Lane. Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33140) at 1/5,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241435230.jpg)
![Immunohistochemistry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunofluorescence analysis of paraffin-embedded human tonsil tissue labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33140, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29820241492693.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Immunocytochemistry analysis of Daudi cells labeling alpha Tubulin with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-alpha Tubulin antibody (NBP3-33140) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-29202410484841.jpg)
![Flow Cytometry: alpha Tubulin Antibody (PSH02-95) [NBP3-33140] - Flow cytometric analysis of HeLa cells labeling alpha Tubulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33140, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33140_rabbit-alpha-tubulin-mab-psh02-95-392024884679.jpg)
![Western Blot: LEDGF Antibody [NB110-40561] - Detection of Human LEDGF/p75 on HeLa whole cell lysate using NB110-40561. LEDGF/p75 was also immunoprecipitated by another rabbit anti-LEDGF/p75 antibody.](http://images.novusbio.com/fullsize/LEDGF-Antibody-Western-Blot-NB110-40561-img0002.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: LEDGF Antibody [NB110-40561] - Detection of Human LEDGF/p75 on HeLa whole cell lysate using NB110-40561. LEDGF/p75 was also immunoprecipitated by another rabbit anti-LEDGF/p75 antibody.")
![Immunocytochemistry/Immunofluorescence: CNTF Antibody [NBP1-83277] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/CNTF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83277-img0006.jpg "Immunocytochemistry/Immunofluorescence: CNTF Antibody [NBP1-83277] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277] - Staining of human Skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/CNTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83277-img0010.jpg)
![Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277] - Staining of human Cerebellum shows moderate cytoplasmic positivity in Purkinje cells.](http://images.novusbio.com/fullsize/CNTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83277-img0008.jpg)
![Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277] - Staining of human Cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/CNTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83277-img0009.jpg)
![Immunohistochemistry-Paraffin: CNTF Antibody [NBP1-83277] - Staining of human Skin shows very weak cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CNTF-Antibody-Immunohistochemistry-Paraffin-NBP1-83277-img0011.jpg)
