| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47948-0.1mg | |||
| IHC-Prediluted | NBP2-48101 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-13963-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48364 | |||
| Azide and BSA Free | NBP3-11532 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NB600-939-0.1mg | |||
| HRP | NB120-7339-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-20224-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21163-100ul | |||
| BSA Free | NBP3-21163-25ul | |||
![Western Blot: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Western blot analysis of extracts of Mouse brain, using IP3R1 Rabbit mAb (NBP3-16414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/IP3R1-Antibody-4Z1B6-Western-Blot-NBP3-16414-img0005.jpg "Western Blot: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Western blot analysis of extracts of Mouse brain, using IP3R1 Rabbit mAb (NBP3-16414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Immunofluorescence analysis of mouse brain using IP3R1 Rabbit mAb (NBP3-16414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IP3R1-Antibody-4Z1B6-Immunohistochemistry-NBP3-16414-img0004.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Immunohistochemistry of paraffin-embedded rat brain using IP3R1 Rabbit mAb (NBP3-16414) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IP3R1-Antibody-4Z1B6-Immunohistochemistry-Paraffin-NBP3-16414-img0001.jpg)
![Immunohistochemistry-Paraffin: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Immunohistochemistry of paraffin-embedded mouse brain using IP3R1 Rabbit mAb (NBP3-16414) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IP3R1-Antibody-4Z1B6-Immunohistochemistry-Paraffin-NBP3-16414-img0002.jpg)
![Immunohistochemistry: IP3R1 Antibody (4Z1B6) [NBP3-16414] - Immunofluorescence analysis of rat brain using IP3R1 Rabbit mAb (NBP3-16414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/IP3R1-Antibody-4Z1B6-Immunohistochemistry-NBP3-16414-img0003.jpg)
![Western Blot: KCNN2 Antibody [NBP2-84112] - Host: Rabbit. Target Name: KCNN2. Sample Type: Human Fetal Muscle. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84112-img0004.jpg "Western Blot: KCNN2 Antibody [NBP2-84112] - Host: Rabbit. Target Name: KCNN2. Sample Type: Human Fetal Muscle. Antibody Dilution: 1.0ug/ml")
![Immunohistochemistry: KCNN2 Antibody [NBP2-84112] - Sample Type: Rhesus macaque spinal cord. Primary Antibody Dilution: 1:300. Secondary Antibody: Donkey anti Rabbit 488. Secondary Antibody Dilution: 1:500. Color/Signal Descriptions: Green: KCNN2. Gene Name: KCNN2. Submitted by: Timur Mavlyutov, Ph. D., Department of Pharmacology, University of Wisconsin Medical School, 1300 University Avenue, Madison, WI 53706](http://images.novusbio.com/fullsize/KCNN2-Antibody-Immunohistochemistry-NBP2-84112-img0006.jpg)
![Western Blot: KCNN2 Antibody [NBP2-84112] - WB Suggested Anti-KCNN2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Muscle](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84112-img0001.jpg)
![Western Blot: KCNN2 Antibody [NBP2-84112] - Host: Rabbit. Target Name: KCNN2. Sample Type: Human Fetal Heart. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84112-img0002.jpg)
![Western Blot: KCNN2 Antibody [NBP2-84112] - Host: Rabbit. Target Name: KCNN2. Sample Type: Human Fetal Lung. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84112-img0003.jpg)
![Western Blot: KCNN2 Antibody [NBP2-84112] - Host: Rabbit. Target: KCNN2. Positive control (+): Human liver (LI). Negative control (-): HeLa (HL). Antibody concentration: 1ug/ml](http://images.novusbio.com/fullsize/KCNN2-Antibody-Western-Blot-NBP2-84112-img0005.jpg)
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody [NLS1040] - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/Melanocortin-1-R-MC1R-Antibody-Immunohistochemistry-Paraffin-NLS1040-img0002.jpg "Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody [NLS1040] - Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE)")
![Immunohistochemistry-Paraffin: Melanocortin-1 R/MC1R Antibody [NLS1040] - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)](http://images.novusbio.com/fullsize/Melanocortin-1-R-MC1R-Antibody-Immunohistochemistry-Paraffin-NLS1040-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799 + KRT19/800) [NBP2-44829] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Cytokeratin 19 Antibody (KRT19/799 + KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-+-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44829-img0006.jpg "Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799 + KRT19/800) [NBP2-44829] - Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with Cytokeratin 19 Antibody (KRT19/799 + KRT19/800)")
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799 + KRT19/800) [NBP2-44829] - Human Bladder Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799 + KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-+-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44829-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799 + KRT19/800) [NBP2-44829] - Human Cervical Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799 + KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-+-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44829-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799 + KRT19/800) [NBP2-44829] - Human Endometrial Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799 + KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-+-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44829-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799 + KRT19/800) [NBP2-44829] - Human Ovarian Carcinoma stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799 + KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-+-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44829-img0004.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (KRT19/799 + KRT19/800) [NBP2-44829] - Human Pancreas stained with Cytokeratin 19 Monoclonal Antibody (KRT19/799 + KRT19/800)](http://images.novusbio.com/fullsize/Cytokeratin-19-Antibody-KRT19-799-+-KRT19-800-Immunohistochemistry-Paraffin-NBP2-44829-img0005.jpg)
![Western Blot: alpha Endosulfine Antibody [NBP2-13963] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/alpha-Endosulfine-Antibody-Western-Blot-NBP2-13963-img0003.jpg "Western Blot: alpha Endosulfine Antibody [NBP2-13963] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: alpha Endosulfine Antibody [NBP2-13963] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize/alpha-Endosulfine-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-13963-img0006.jpg)
![Immunohistochemistry-Paraffin: alpha Endosulfine Antibody [NBP2-13963] - Staining of human esophagus shows moderate cytoplasmic and nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/alpha-Endosulfine-Antibody-Immunohistochemistry-Paraffin-NBP2-13963-img0005.jpg)
![Western Blot: Spectrin beta 2 Antibody [NB100-40828] - Detection of mouse SPTBN1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN1 antibody NB100-40828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Western-Blot-NB100-40828-img0005.jpg "Western Blot: Spectrin beta 2 Antibody [NB100-40828] - Detection of mouse SPTBN1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN1 antibody NB100-40828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry: Spectrin beta 2 Antibody [NB100-40828] - Detection of human SPTBN1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- SPTBN1 (NB100-40828). Detection: DAB](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Immunohistochemistry-NB100-40828-img0006.jpg)
![Western Blot: Spectrin beta 2 Antibody [NB100-40828] - Detection of human SPTBN1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SPTBN1 antibody NB100-40828 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Western-Blot-NB100-40828-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Spectrin beta 2 Antibody [NB100-40828] - Detection of human Spectrin beta 2 (SPTBN1) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Spectrin beta 2 antibody NB100-40828 used for IP at 3 ug per reaction. SPTBN1 was also immunoprecipitated by rabbit anti-Spectrin beta 2 antibody NB100-40829. For blotting immunoprecipitated SPTBN1, NB100-40828 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/Spectrin-beta-2-Antibody-Immunoprecipitation-NB100-40828-img0007.jpg)
![Immunohistochemistry-Paraffin: CD34 Antibody (SPM610) [NBP2-44570] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Antibody (SPM610)](http://images.novusbio.com/fullsize/CD34-Antibody-SPM610-Immunohistochemistry-Paraffin-NBP2-44570-img0002.jpg "Immunohistochemistry-Paraffin: CD34 Antibody (SPM610) [NBP2-44570] - Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Antibody (SPM610)")
![Immunohistochemistry-Paraffin: CD34 Antibody (SPM610) [NBP2-44570] - Human Angiosarcoma stained with CD34 Monoclonal Antibody (SPM610)](http://images.novusbio.com/fullsize/CD34-Antibody-SPM610-Immunohistochemistry-Paraffin-NBP2-44570-img0001.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ug per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight (TM) 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block for30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.](http://images.novusbio.com/fullsize/alpha 2-Macroglobulin Antibody-Western Blot-NB600-938-img0010.jpg "Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ug per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight (TM) 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block for30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.")
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Analysis using the Biotin conjugate of NB600-938. Detection of Lane 1: . Lane 2: none. Load: 100 ug per lane. Primary antibody:alpha 2-Macroglobulin biotin conjugated antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Western-Blot-NB600-938-img0006.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Detection of Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of 1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat 1 hr RT.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Western-Blot-NB600-938-img0007.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody [NB600-938] - Lane 1: Alpha-2-Macroglobulin. Lane 2: None. Load: 50 ng per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 163 kDa, 163 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-Western-Blot-NB600-938-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: TRPM7 Antibody [NBP1-20224] - PC12 cells at a concentration of 30 ug/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG , DAPI counterstained.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-20224-img0005.jpg "Immunocytochemistry/Immunofluorescence: TRPM7 Antibody [NBP1-20224] - PC12 cells at a concentration of 30 ug/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG , DAPI counterstained.")
![Immunocytochemistry/Immunofluorescence: TRPM7 Antibody [NBP1-20224] - IF on PC12 cells at a concentration of 30 ug/ml using Rabbit antibody to 4th cytoplasmic loop of human TRPM7 (LTRPC7): IgG , DAPI counterstained appearing in blue.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-20224-img0004.jpg)
![Western Blot: GAPDH Antibody (RM114) [NBP2-77402] - Western blot of A431 cells, using NBP2-77402, showed a band of GAPDH at the predicted MW (~36 kDa).](http://images.novusbio.com/fullsize/GAPDH-Antibody-RM114-Western-Blot-NBP2-77402-img0002.jpg "Western Blot: GAPDH Antibody (RM114) [NBP2-77402] - Western blot of A431 cells, using NBP2-77402, showed a band of GAPDH at the predicted MW (~36 kDa).")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody (RM114) [NBP2-77402] - Immunocytochemical staining of HeLa cells, using NBP2-77402 (red). Nuclei have been labeled with DAPI (blue).](http://images.novusbio.com/fullsize/nbp2-77402_rabbit-monoclonal-gapdh-antibody-rm114-immunocytochemistry-immunofluorescence-199202502434.png)
![Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1470] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48655_rabbit-melanocortin-1-r-mc1r-mab-hl1470-western-blot-2592025864.png "Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MC1 Receptor antibody [HL1470] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1470] (NBP3-48655) diluted at 1:50000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48655_rabbit-melanocortin-1-r-mc1r-mab-hl1470-western-blot-141120251831322.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Melanocortin-1 R/MC1R antibody [HL1470] (NBP3-48655) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-48655_rabbit-melanocortin-1-r-mc1r-mab-hl1470-western-blot-141120251828111.jpg)
![<b>Independent Antibodies Validation. </b>Various tissue extracts (50 ug) was separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1470] (NBP3-48655) diluted at 1:1000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated with and does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48655_rabbit-melanocortin-1-r-mc1r-mab-hl1470-western-blot-141120251831212.jpg)
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Non-transfected (-) and transfected (+) unboiled A375 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membranes were blotted with Melanocortin-1 R/MC1R antibody [HL1470] (NBP3-48655) diluted at 1:5000 and competitor's antibody (Highly Cited Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-48655_rabbit-melanocortin-1-r-mc1r-mab-hl1470-western-blot-141120251836367.jpg)