| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54578-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50033SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50057SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-29623SS | |||
| Azide and BSA Free | NBP2-80957 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-155SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-60735SS | |||
| BSA Free | NBP2-60735 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44055 | |||
| BSA Free | NBP2-52874-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76798SS | |||
| Azide and BSA Free | NBP2-80879 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80849-25ul | |||
![Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Western Blot Analysis of human spleen tissue lysate using B220/CD45R antibody (PTPRC/1783R).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Western-Blot-NBP2-53303-img0006.jpg "Western Blot: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Western Blot Analysis of human spleen tissue lysate using B220/CD45R antibody (PTPRC/1783R).")
![Immunocytochemistry/Immunofluorescence: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Paraformaldehyde-fixed Raji cells stained with B220/CD45R Antibody (PTPRC/1783R) followed by Goat anti-rabbit IgG-CF488 (Green). Counterstain is Phalloidin (red).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Immunocytochemistry-Immunofluorescence-NBP2-53303-img0003.jpg)
![Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - FFPE section of human lung cancer. 10X magnification. IHC-P image submitted by a verified customer review.](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Immunohistochemistry-Paraffin-NBP2-53303-img0004.jpg)
![Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Flow Cytometric Analysis of PFA-fixed Raji cells. B220/CD45R antibody (PTPRC/1783R) followed by goat anti-rabbit IgG-CF488 (blue); Isotype Control (red).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Flow-Cytometry-NBP2-53303-img0005.jpg)
![Immunohistochemistry-Paraffin: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Formalin-fixed paraffin-embedded human Tonsil stained with CD45RB Recombinant Rabbit Monoclonal Antibody (PTPRC/1783R).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Immunohistochemistry-Paraffin-NBP2-53303-img0001.jpg)
![Flow Cytometry: B220/CD45R Antibody (PTPRC/1783R) [NBP2-53303] - Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells using B220/CD45R Antibody (PTPRC/1783R) followed by Goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/B220-CD45R-Antibody-PTPRC-1783R-Flow-Cytometry-NBP2-53303-img0002.jpg)
![Western Blot: ALDH1L1 Antibody (2E7) [NBP2-50033] - Analysis of different tissue and cell lysates using mouse mAb to ALDH1L1, NBP2-50033, dilution 1:5,000 in green: [1] protein standard (red), rat tissue lysates: [2] heart, [3] liver, [4] kidney,[5] lung, [6] brain, and [7] spinal cord; mouse tissue lysates: [8] brain, and [9] spinal cord; cell lysates: [10] NIH-3T3, and [11] HEK293. The band at 100kDa mark corresponds to ALDH1L1 protein.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-2E7-Western-Blot-NBP2-50033-img0003.jpg "Western Blot: ALDH1L1 Antibody (2E7) [NBP2-50033] - Analysis of different tissue and cell lysates using mouse mAb to ALDH1L1, NBP2-50033, dilution 1:5,000 in green: [1] protein standard (red), rat tissue lysates: [2] heart, [3] liver, [4] kidney,[5] lung, [6] brain, and [7] spinal cord; mouse tissue lysates: [8] brain, and [9] spinal cord; cell lysates: [10] NIH-3T3, and [11] HEK293. The band at 100kDa mark corresponds to ALDH1L1 protein.")
![Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033] - Analysis of cortical neuron-glial cell culture from E20 rat stained with mouse mAb to aldehyde dehydrogenase 1L1 (ALDH1L1), NBP2-50033, dilution 1:1,000 in red, and costained with chicken pAb to GFAP, dilution 1:5,000 in green. The blue is Hoechst staining of nuclear DNA. NBP2-50033 antibody produces cytoplasmic staining of glial cells, while the GFAP antibody labels the intermediate filament cytoskeleton in astrocytes and other glial cells. Some astrocytic cells express both ALDH1L1 and GFAP and appear yellow.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-2E7-Immunocytochemistry-Immunofluorescence-NBP2-50033-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: ALDH1L1 Antibody (2E7) [NBP2-50033] - Neuron-glia cell mixed cultures stained with NBP2-50033 (red) and chicken polyclonal antibody against Vimentin (NB300-223, green). Blue is a DNA stain. NBP2-50033 stains astrocytic cell bodies and processes. The fibroblastic cells contain only Vimentin and so are green, while astrocytes contain either Vimentin and ALDH1L1, so appearing golden, or predominantly ALDH1L1, in which case they appear red.](http://images.novusbio.com/fullsize/ALDH1L1-Antibody-2E7-Immunocytochemistry-Immunofluorescence-NBP2-50033-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP2-50057] - Analysis of HeLa cells stained with rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000 in red, and mouse mAb to tubulin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. HeLa cells were kept in fetal bovine serum (FBS) free media for 36 hours. Then the cells were treated with PBS (A), as a control, or stimulated with 20% FBS (B) for 30 min. c-FOS antibody labels only the nuclei of stimulated cells. Mouse hippocampus (C) or olfactory bulb sections (D) stained with NBP2-50057, dilution 1:20,000 in red, and mouse mAb to NF-L, dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.](http://images.novusbio.com/fullsize/c-Fos-Antibody-Western-Blot-NBP2-50057-img0001.jpg "Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NBP2-50057] - Analysis of HeLa cells stained with rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000 in red, and mouse mAb to tubulin, dilution 1:10,000, in green. The blue is DAPI staining of nuclear DNA. HeLa cells were kept in fetal bovine serum (FBS) free media for 36 hours. Then the cells were treated with PBS (A), as a control, or stimulated with 20% FBS (B) for 30 min. c-FOS antibody labels only the nuclei of stimulated cells. Mouse hippocampus (C) or olfactory bulb sections (D) stained with NBP2-50057, dilution 1:20,000 in red, and mouse mAb to NF-L, dilution 1:5,000, in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The c-FOS antibody stains only nuclei of spontaneously active neurons. NF-L is expressed in axons of neuronal cells.")
![Western Blot: c-Fos Antibody [NBP2-50057] - Analysis of cell lysates using rabbit pAb to c-FOS, NBP2-50057, dilution 1:5,000, in green, and mouse mAb to GAPDH, dilution 1:5,000, in red, used as a loading control. [1] protein standard (red), [2] HeLa cells grown in FBS free media, [3] HeLa cells stimulated with 20% FBS for 2 hours after being in FBS free media for 36 hours, [4] rat cortical neurons, [5] rat cortical neurons treated with membrane depolarization buffer for 5 hours. Multiple bands at 50-65 kDa in stimulated or treated cell lysates, correspond to different isoforms of the c-FOS protein](http://images.novusbio.com/fullsize/c-Fos-Antibody-Western-Blot-NBP2-50057-img0002.jpg)
![Western Blot: SOX2 Antibody (4G8) [NBP2-29623] - Western blot detection of SOX2 in A. HEK293 cell lysate B. NCCIT cell lysate and C. Full length recombinant protein.](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Western-Blot-NBP2-29623-img0001.jpg "Western Blot: SOX2 Antibody (4G8) [NBP2-29623] - Western blot detection of SOX2 in A. HEK293 cell lysate B. NCCIT cell lysate and C. Full length recombinant protein.")
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human lung cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a punctate immunopositivity of SOX2 protein in the cytoplasm and the staining was mainly localized in the perinuclear region of the cells [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0013.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC-P analysis of SOX2 protein in a section of human kidney using 5 ug/ml concentration of SOX2 antibody (clone 4G8). The representative image shows distinct nuclear and cytoplasmic staining pattern in cells of different types of renal tubules and blood vessels in the kidney section [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0002.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC-P analysis of SOX2 protein in a section of human renal cell carcinoma using 5 ug/ml concentration of SOX2 antibody (clone 4G8). Granular nuclear and cytoplasmic staining pattern was observed in the carcinoma cells, whereas, a weak to negligible staining was observed in the stroma areas [Magnification 40X]..](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0003.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human liver using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The liver section depicted a diffused cytoplasmic staining in the hepatocytes [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0004.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human brain using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The representative photomicrograph depicts a cytoplasmic and nuclear immunopositivity of SOX2 protein in brain [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0006.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded section of normal human colon tissue using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. A weak and diffused cytoplasmic staining was observed in majority of the cells in mucosal layer of colon [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0007.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human bladder cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a cytoplasmic and nuclear immunepositivity for SOX2 protein [Magnification 10X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0008.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human breast cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a cytoplasmic and nuclear immunepositivity, whereas the stroma was largely negative for SOX2 protein [Magnification 10X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0009.jpg)
![Immunohistochemistry-Paraffin: SOX2 Antibody (4G8) [NBP2-29623] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human uterine cancer using mouse monoclonal SOX2 antibody (clone 4G8) at 5 ug/ml concentration. The cancer cells developed a punctate staining in the cytoplasm and nuclei, whereas the stroma was very weakly positive for SOX2 protein [Magnification 40X].](http://images.novusbio.com/fullsize/SOX2-Antibody-4G8-Immunohistochemistry-Paraffin-NBP2-29623-img0010.jpg)
![Western Blot: Neurofibromin 1 Antibody [NB300-155] - Western Blot of (A, B, C above).](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Western-Blot-NB300-155-img0004.jpg "Western Blot: Neurofibromin 1 Antibody [NB300-155] - Western Blot of (A, B, C above).")
![Immunohistochemistry: Neurofibromin 1 Antibody [NB300-155] - NF1 protein labeling displays positive signal in the maturing and the hypertrophic cartilages but only faint labeling in the proliferative cartilage.](http://images.novusbio.com/fullsize/Neurofibromin-1-Antibody-Immunohistochemistry-NB300-155-img0003.jpg)
![SDS-Page: Recombinant Human DRP1 Protein [H00010059-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-DRP1-Protein-SDS-Page-H00010059-P01-img0002.jpg "SDS-Page: Recombinant Human DRP1 Protein [H00010059-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: HMGB1/HMG-1 Antibody (1D5) [H00003146-M02] - Analysis of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody (M02), clone 1D5.Lane 1: HMGB1 transfected lysate(24.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1D5-Western-Blot-H00003146-M02-img0015.jpg "Western Blot: HMGB1/HMG-1 Antibody (1D5) [H00003146-M02] - Analysis of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody (M02), clone 1D5.Lane 1: HMGB1 transfected lysate(24.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: HMGB1/HMG-1 Antibody (1D5) [H00003146-M02] - Analysis of monoclonal antibody to HMGB1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1D5-Immunocytochemistry-Immunofluorescence-H00003146-M02-img0011.jpg)
![Immunohistochemistry-Paraffin: HMGB1/HMG-1 Antibody (1D5) [H00003146-M02] - Analysis of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human hepatocellular carcinoma tissue. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1D5-Immunohistochemistry-Paraffin-H00003146-M02-img0013.jpg)
![Western Blot: HMGB1/HMG-1 Antibody (1D5) [H00003146-M02] - HMGB1 monoclonal antibody (M02), clone 1D5 Analysis of HMGB1 expression in K-562.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1D5-Western-Blot-H00003146-M02-img0014.jpg)
![Sandwich ELISA Capture: HMGB1/HMG-1 Antibody (1D5) [H00003146-M02] - Detection limit for recombinant GST tagged HMGB1/HMG-1 is 0.1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HMGB1-HMG-1-Antibody-1D5-Sandwich-ELISA-Capture-H00003146-M02-img0016.jpg)
![Western Blot: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Western-Blot-NBP2-80831-img0001.jpg "Western Blot: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Total protein from HeLa and Neuro2A cells treated with or without 50 uM chloroquine (CQ) for 24 hours was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF (Polyvinylidene difluoride) membrane and blocked in 5% non-fat milk in TBST (Tris-buffered saline, 0.1% Tween 20). The membrane was probed with 12.0 ug/ml anti-LC3B Antibody (1251B) [Catalog # NBP2-60735] in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Image from the standard format of this antibody.")
![Immunocytochemistry/Immunofluorescence: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - HeLa cells were treated with 50uM CQ (Chloroquine) overnight, fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti- [Catalog # NBP2-60735] at 2 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-80831-img0002.jpg)
![Immunohistochemistry: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Analysis of a FFPE (Formalin-Fixed Paraffin-Embedded) tissue section of mouse brain using anti-LC3B antibody (clone 1251B) [Catalog # NBP2-60735] at 5ug/ml concentration (1:200 dilution). The primary antibody binding to LC3 in cells was detected using HRP](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Immunohistochemistry-NBP2-80831-img0003.jpg)
![<b>Biological Strategies Validation. </b>Simple Western: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 50uM Chloroquine for 18 hours, loaded at 0.2 mg/mL. A specific band was detected for LC3B at approximately 17 kDa (as indicated) using 5 ug/mL of Rabbit Anti-Human/Mouse/Rat anti-LC3B Monoclonal Antibody (1251B) [Catalog # NBP2-60735]. This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Simple-Western-NBP2-80831-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: LC3B Antibody (1251B) - Azide and BSA Free [NBP2-80831] - Western blot analysis of HeLa human cervical epithelial carcinoma parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50uM Chloroquine for 18 hours. PVDF (polyvinylidene difluoride) membrane was probed with 0.1 ug/mL of anti-LC3B monoclonal antibody (Catalog # NBP2-60735) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for LC3B at approximately 15 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. Image from the standard format of this antibody.](http://images.novusbio.com/fullsize/LC3B-Antibody-1251B-Azide-and-BSA-Free-Knockout-Validated-NBP2-80831-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Immunofluorescence of HeLa wildtype (WT) and FUS knockout (KO) cells labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio on coverslips. Cells were stained with Mouse Anti-FUS Monoclonal Antibody (Catalog #NBP2-52874) at 1:1000, followed by the corresponding Alexa Fluor 555-coupled Secondary Antibody. Acquisition of the green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative image of the red (grayscale) channel is shown. WT and KO cells are outlined with yellow and magenta dashed line, respectively. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0021.jpg "<b>Genetic Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Immunofluorescence of HeLa wildtype (WT) and FUS knockout (KO) cells labelled with a green or a far-red fluorescent dye, respectively. WT and KO cells were mixed and plated to a 1:1 ratio on coverslips. Cells were stained with Mouse Anti-FUS Monoclonal Antibody (Catalog #NBP2-52874) at 1:1000, followed by the corresponding Alexa Fluor 555-coupled Secondary Antibody. Acquisition of the green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative image of the red (grayscale) channel is shown. WT and KO cells are outlined with yellow and magenta dashed line, respectively. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Western Blot: FUS Antibody (CL0190) [NBP2-52874] - Western blot using lysates of HeLa parental cell line (WT) and FUS knockout HeLa cell line (KO), collected in RIPA Buffer. Lysates were prepared using 30 ug of protein and the nitrocellulose membrane was probed with Mouse Anti-FUS Monoclonal Antibody (Catalog #NBP2-52874) at 1:5000 O/N at 4C, followed by peroxidase-conjugated Anti-Mouse Secondary Antibody and ECL detection. A specific band at ~70 kDa was detected in the FUS WT cell line but is not observed in the FUS KO cell line. The Ponceau stained transfer of the blot is shown to confirm equal protein loading. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Western-Blot-NBP2-52874-img0023.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining in human cerebral cortex and liver tissues. Corresponding FUS RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunohistochemistry-Paraffin-NBP2-52874-img0020.jpg)
![<b>Genetic Strategies Validation. </b>Knockdown Validated: FUS Antibody (CL0190) [NBP2-52874] - Analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-FUS monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Western-Blot-NBP2-52874-img0014.jpg)
![Western Blot: FUS Antibody (CL0190) [NBP2-52874] - Lane 1: Marker [kDa], Lane 2: Human cell line HeLa cytoplasmic fraction , Lane 3: Human cell line HeLa membrane fraction , Lane 4: Human cell line HeLa nuclear fraction , Lane 5: Human cell line HeLa chromatin fraction , Lane 6: Human cell line HeLa cytos](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Western-Blot-NBP2-52874-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in U2OS cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0005.jpg)
![Western Blot: FUS Antibody (CL0190) [NBP2-52874] - Lane 1: Marker [kDa]Lane 2: Human cell line HeLaLane 3: Human cell line A-431Lane 4: Human cell line MCF-7Lane 5: Human cell line U2-OSLane 6: Human cell line Hep-G2](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Western-Blot-NBP2-52874-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in A431 cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in HeLa cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in MCF7 cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: FUS Antibody (CL0190) [NBP2-52874] - Staining in U251 cell line with Anti-FUS monoclonal antibody, showing clear nuclear (without nucleoli) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunocytochemistry-Immunofluorescence-NBP2-52874-img0004.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human endometrium shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunohistochemistry-Paraffin-NBP2-52874-img0016.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunohistochemistry-Paraffin-NBP2-52874-img0017.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunohistochemistry-Paraffin-NBP2-52874-img0018.jpg)
![Immunohistochemistry-Paraffin: FUS Antibody (CL0190) [NBP2-52874] - Staining of human liver shows only very weak nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/FUS-Antibody-CL0190-Immunohistochemistry-Paraffin-NBP2-52874-img0019.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Knockout-Validated-NBP2-76798-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti- Niemann-Pick C1 Antibody at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0006.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Total protein from human HeLa, Hek293, mouse 3T3 and Rat PC12 cell lines were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Western-Blot-NBP2-76798-img0005.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Niemann-Pick Type C1/NPC1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (NBP2-76798) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic puncta.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunohistochemistry-Paraffin-NBP2-76798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Niemann-Pick C1 Antibody (1318A) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0004.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Analysis in human adrenal gland and tonsil tissues using NBP1-80849 antibody. Corresponding RGN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0024.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Analysis in human adrenal gland and tonsil tissues using NBP1-80849 antibody. Corresponding RGN RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human adrenal gland, kidney, liver and tonsil using Anti-RGN antibody NBP1-80849 (A) shows similar protein distribution across tissues to independent antibody NBP1-80850 (B).](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0023.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: Regucalcin Antibody [NBP1-80849] - Analysis using Anti-RGN antibody NBP1-80849 (A) shows similar pattern to independent antibody NBP1-80850 (B).](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Western-Blot-NBP1-80849-img0019.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0027.jpg)
![Western Blot: Regucalcin Antibody [NBP1-80849] - Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate. Recommended dilution: 1:250 - 1:500](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Western-Blot-NBP1-80849-img0004.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human adrenal gland shows strong positivity in glandular cells.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0025.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human liver shows strong positivity in hepatocytes.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0026.jpg)
![Immunohistochemistry-Paraffin: Regucalcin Antibody [NBP1-80849] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/Regucalcin-Antibody-Immunohistochemistry-Paraffin-NBP1-80849-img0028.jpg)
![ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62767] - Samples were spiked with high concentrations of Mouse HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-62767_mouse-hmgb1-hmg-1-elisa-kit-colorimetric-132202416173468.jpg "ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62767] - Samples were spiked with high concentrations of Mouse HMGB1/HMG-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse HMGB1/HMG-1 ELISA Kit (Colorimetric) [NBP2-62767] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-62767_mouse-hmgb1-hmg-1-elisa-kit-colorimetric-4320249115619.jpg)