| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-82364SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44831 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03221-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61842-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61121-0.025ml | |||
![Western Blot: TRPM2 Antibody [NB110-82364] - Detection of TRPM2 in mouse brain membrane lysates using NB110-82364.](http://images.novusbio.com/fullsize/TRPM2-Antibody-Western-Blot-NB110-82364-img0002.jpg "Western Blot: TRPM2 Antibody [NB110-82364] - Detection of TRPM2 in mouse brain membrane lysates using NB110-82364.")
![Immunocytochemistry/Immunofluorescence: TRPM2 Antibody [NB110-82364] - TRPM2 antibody was tested in Neuro2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/TRPM2-Antibody-Immunocytochemistry-Immunofluorescence-NB110-82364-img0004.jpg)
![Western Blot: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (SPM576).](http://images.novusbio.com/fullsize/Vimentin-Antibody-SPM576-Azide-and-BSA-Free-Western-Blot-NBP2-34809-img0001.jpg "Western Blot: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (SPM576).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Formalin-paraffin human melanoma stained with Vimentin MAb (SPM576).](http://images.novusbio.com/fullsize/Vimentin-Antibody-SPM576-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34809-img0002.jpg)
![Western Blot: Glutamate Receptor 6 Antibody (4B4L9) [NBP3-16867] - Western blot analysis of extracts of Mouse brain, using Glutamate Receptor 6 Rabbit mAb (NBP3-16867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Glutamate-Receptor-6-Antibody-4B4L9-Western-Blot-NBP3-16867-img0004.jpg "Western Blot: Glutamate Receptor 6 Antibody (4B4L9) [NBP3-16867] - Western blot analysis of extracts of Mouse brain, using Glutamate Receptor 6 Rabbit mAb (NBP3-16867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western Blot: Glutamate Receptor 6 Antibody (4B4L9) [NBP3-16867] - Western blot analysis of extracts of 293T cells, using Glutamate Receptor 6 Rabbit mAb (NBP3-16867) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Glutamate-Receptor-6-Antibody-4B4L9-Western-Blot-NBP3-16867-img0005.jpg)
![Immunohistochemistry: Glutamate Receptor 6 Antibody (4B4L9) [NBP3-16867] - Immunohistochemistry analysis of Glutamate Receptor 6 in paraffin-embedded human colon carcinoma tissue using Glutamate Receptor 6 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16867_rabbit-glutamate-receptor-6-mab-4b4l9-822025201041.jpg)
![Immunohistochemistry: Glutamate Receptor 6 Antibody (4B4L9) [NBP3-16867] - Immunohistochemistry analysis of Glutamate Receptor 6 in paraffin-embedded human kidney tissue using Glutamate Receptor 6 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16867_rabbit-glutamate-receptor-6-mab-4b4l9-822025208497.jpg)
![Immunohistochemistry: Glutamate Receptor 6 Antibody (4B4L9) [NBP3-16867] - Immunohistochemistry analysis of Glutamate Receptor 6 in paraffin-embedded mouse intestin tissue using Glutamate Receptor 6 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16867_rabbit-glutamate-receptor-6-mab-4b4l9-822025209849.jpg)
![Immunohistochemistry: Glutamate Receptor 6 Antibody (4B4L9) [NBP3-16867] - Immunohistochemistry analysis of Glutamate Receptor 6 in paraffin-embedded rat kidney tissue using Glutamate Receptor 6 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16867_rabbit-glutamate-receptor-6-mab-4b4l9-822025208480.jpg)
![Western Blot: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Western blot analysis of various lysates, using Aquaporin-4Rabbit mAb at 1:900 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-912202414154378.jpg "Western Blot: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Western blot analysis of various lysates, using Aquaporin-4Rabbit mAb at 1:900 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.")
![Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-31120251744416.jpg)
![Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-31120251704787.jpg)
![Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-3112025173144.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Human lung cancer tissue using Aquaporin-4 (AQP4) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-35055_rabbit-aquaporin-4-mab-5a4w4-immunohistochemistry-paraffin-1352025122932..png)
![Western Blot: Kir6.1 Overexpression Lysate (Native) [NBL1-12174] - 1. Negative control, 2. HEK cells overexpressing Kir6.1. 15ug lysate each was loaded onto a 10% SDS-polyacrylamide gel and transferred to a PVDF membrane, and incubated with an anti-Kir6.1 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Kir6-1-Overexpression-Lysate-Native-Western-Blot-NBL1-12174-img0003.jpg "Western Blot: Kir6.1 Overexpression Lysate (Native) [NBL1-12174] - 1. Negative control, 2. HEK cells overexpressing Kir6.1. 15ug lysate each was loaded onto a 10% SDS-polyacrylamide gel and transferred to a PVDF membrane, and incubated with an anti-Kir6.1 antibody. Image from verified customer review.")
![Western Blot: Kir6.1 Overexpression Lysate (Adult Normal) [NBL1-12174] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Kir6.1.](http://images.novusbio.com/fullsize/Kir6-1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12174-img0002.jpg)
![Western Blot: APP Antibody (3A8S2) [NBP3-15560] - Western blot analysis of extracts of various cell lines, using APP antibody (NBP3-15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/APP-Antibody-3A8S2-Western-Blot-NBP3-15560-img0006.jpg "Western Blot: APP Antibody (3A8S2) [NBP3-15560] - Western blot analysis of extracts of various cell lines, using APP antibody (NBP3-15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: APP Antibody (3A8S2) [NBP3-15560] - Immunofluorescence analysis of HeLa cells using APP Rabbit mAb (NBP3-15560) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/APP-Antibody-3A8S2-Immunocytochemistry-Immunofluorescence-NBP3-15560-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: APP Antibody (3A8S2) [NBP3-15560] - Western blot analysis of extracts from normal (control) and APP knockout (KO) 293T cells, using APP antibody (NBP3-15560) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/APP-Antibody-3A8S2-Knockout-Validated-NBP3-15560-img0001.jpg)
![Immunoprecipitation: APP Antibody (3A8S2) [NBP3-15560] - Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug APP antibody (NBP3-15560). Western blot was performed from the immunoprecipitate using APP antibody (NBP3-15560) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/APP-Antibody-3A8S2-Immunoprecipitation-NBP3-15560-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: APP Antibody (3A8S2) [APP] - Confocal imaging of paraffin-embedded 5XFAD Mouse brain and Mouse brain using [KO Validated] APP Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x.](http://images.novusbio.com/fullsize/nbp3-15560_rabbit-app-mab-3a8s2-52202517304935.jpg)
![Immunohistochemistry:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]](http://images.novusbio.com/fullsize/nbp3-44862_mouse-tyrosine-hydroxylase-mab-oti2d4-immunohistochemistry-181202616152120.jpg "Immunohistochemistry:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]")
![Western Blot:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]](http://images.novusbio.com/fullsize/nbp3-44862_mouse-tyrosine-hydroxylase-mab-oti2d4-western-blot-18120261619287.jpg)
![Western Blot:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]](http://images.novusbio.com/fullsize/nbp3-44862_mouse-tyrosine-hydroxylase-mab-oti2d4-western-blot-18120261615213.jpg)
![Immunohistochemistry:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]](http://images.novusbio.com/fullsize/nbp3-44862_mouse-tyrosine-hydroxylase-mab-oti2d4-immunohistochemistry-18120261618187.jpg)
![Immunofluorescence:Tyrosine Hydroxylase Antibody (OTI2D4)[NBP3-44862]](http://images.novusbio.com/fullsize/nbp3-44862_mouse-tyrosine-hydroxylase-mab-oti2d4-immunofluorescence-181202616154110.jpg)
![Western Blot: MT-CO2 Antibody (6H0N5) [NBP3-16283] - Western blot analysis of extracts of various cell lines, using MT-CO2 Rabbit mAb (NBP3-16283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/MT-CO2-Antibody-6H0N5-Western-Blot-NBP3-16283-img0002.jpg "Western Blot: MT-CO2 Antibody (6H0N5) [NBP3-16283] - Western blot analysis of extracts of various cell lines, using MT-CO2 Rabbit mAb (NBP3-16283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunohistochemistry-Paraffin: MT-CO2 Antibody (6H0N5) [NBP3-16283] - Human liver tissue using MTCO2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16283_rabbit-mt-co2-mab-6h0n5-immunohistochemistry-paraffin-68202495546..jpg)
![Immunohistochemistry- MT-CO2 Antibody (6H0N5) [NBP3-16283] -Confocal imaging of HeLa cells using MTCO2 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16283_rabbit-mt-co2-mab-6h0n5-immunohistochemistry-68202495946..jpg)
![Immunohistochemistry: MT-CO2 Antibody (6H0N5) [NBP3-16283] - Immunohistochemistry analysis of paraffin-embedded Human brain tissue using MT-CO2 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16283_rabbit-mt-co2-mab-6h0n5-52202511411478.jpg)
![Immunocytochemistry/ Immunofluorescence: MT-CO2 Antibody (6H0N5) [NBP3-16283] - Confocal imaging of HeLa cells using MT-CO2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16283_rabbit-mt-co2-mab-6h0n5-5220251255544.jpg)
![Immunocytochemistry/ Immunofluorescence: MT-CO2 Antibody (6H0N5) [MT-CO2] - Confocal imaging of paraffin-embedded Human liver tissue using MT-CO2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16283_rabbit-mt-co2-mab-6h0n5-5220251733582.jpg)
![Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Western-Blot-NBP3-03221-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: Vimentin Antibody [NBP3-03221] - Immunohistochemistry of paraffin-embedded human colon using [KO Validated] Vimentin Rabbit pAb (NBP3-03221) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Vimentin-Antibody-Immunohistochemistry-Paraffin-NBP3-03221-img0002.jpg)
![Western Blot: Vimentin Antibody [NBP3-03221] - Western blot analysis of extracts of various cell lines, using Vimentin antibody (NBP3-03221) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Vimentin Antibody-Western Blot-NBP3-03221-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody - Azide and BSA Free [Vimentin] - Immunofluorescence analysis of U2OS cells using [KD Validated] Vimentin Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03221_rabbit-polyclonal-vimentin-antibody-5220251628096.jpg)
![Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Immunohistochemistry analysis of rat brain injected with Type 1 mouse alpha synuclein PFFs (NBP2-61596). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 mouse alpha synuclein PFFs (NBP2-61596) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-61596-img0003.jpg "Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Immunohistochemistry analysis of rat brain injected with Type 1 mouse alpha synuclein PFFs (NBP2-61596). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 mouse alpha synuclein PFFs (NBP2-61596) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.")
![In vitro assay: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Active alpha synuclein aggregate seeds the formation of new alpha Synuclein aggregates from the pool of active monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate is combined with 100 uM of active alpha Synuclein monomer, as compared to active alpha Synuclein aggregate and active alpha Synuclein monomer alone. Thioflavin T excitation maximum = 450 nm; emission maximum = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-61596-img0002.jpg)
![Electron Microscopy: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TEM of Type 1 mouse alpha synuclein Pre-formed Fibrils (NBP2-61596). Fibrils were sonicated and image was taken at 100kx magnification.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-61596-img0005.jpg)
![Electron Microscopy: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TEM of Type 1 mouse alpha synuclein Pre-formed Fibrils (NBP2-61596). Image was taken at 100kx magnification.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-61596-img0004.jpg)
![Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TH fluorescence in mouse brain. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-61596_recombinant-mouse-alpha-synuclein-active-pre-formed-fibrils-type-1-protein-immunohistochemistry-205202512497..jpg)
![Western Blot: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis using GRM3 mAb against HEK293 (1) and GRM3 (AA: extra 433-576)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-Western-Blot-NBP2-61842-img0005.jpg "Western Blot: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis using GRM3 mAb against HEK293 (1) and GRM3 (AA: extra 433-576)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis of paraffin-embedded stomach cancer tissues using GRM3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-Immunohistochemistry-NBP2-61842-img0004.jpg)
![Western Blot: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis using GRM3 mAb against human GRM3 (AA: extra 433-576) recombinant protein. (Expected MW is 42.4 kDa)](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-Western-Blot-NBP2-61842-img0006.jpg)
![Immunohistochemistry: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis of paraffin-embedded bladder cancer tissues using GRM3 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-Immunohistochemistry-NBP2-61842-img0003.jpg)
![ELISA: mGluR3 Antibody (6H10C3) [NBP2-61842] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-ELISA-NBP2-61842-img0001.jpg)
![ELISA: mGluR3 Antibody (6H10C3) [NBP2-61842] - Analysis of SH-SY5Y cells using GRM3 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/mGluR3-Antibody-6H10C3-ELISA-NBP2-61842-img0002.jpg)
![Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Western-Blot-NBP2-61121-img0004.jpg "Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Phospho serine 129 antibody (NBP2-61121) was used to detect phosphorylated alpha synuclein in primary mouse hippocampal neurons treated with 100 nM sonicated mouse alpha synuclein PFFs (A). Phosphorylated alpha synuclein was visible in perinucleus and neurites compared to untreated control (B). Read the protocol at pabmabs.com/?p=7901. Image courtesy of Trine Rasmussen, Simon Molgaard Jensen at Aarhus University.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0006.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunohistochemistry analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunohistochemistry-NBP2-61121-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 g/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:500 for 24 hours at 4C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/alpha-Synuclein [p Ser129] Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0007.jpg)