| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34215-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59329-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81588-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-39000-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83162-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90250-25ul | |||
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Analysis in human cerebral cortex and pancreas tissues using NBP2-34215 antibody. Corresponding Synaptotagmin 1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Analysis in human cerebral cortex and pancreas tissues using NBP2-34215 antibody. Corresponding Synaptotagmin 1 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human cerebellum, liver, pancreas and testis using Anti-Synaptotagmin 1 antibody NBP2-34215 (A) shows similar protein distribution across tissues to independent antibody NBP1-87362 (B).](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0017.jpg)
![Western Blot: Synaptotagmin 1 Antibody [NBP2-34215] - Analysis in control (vector only transfected HEK293T lysate) and SYT1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Western-Blot-NBP2-34215-img0011.jpg)
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human cerebral cortex shows high expression.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0008.jpg)
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human liver.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0013.jpg)
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human testis.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0014.jpg)
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human cerebral cortex using Anti-SYT1 antibody NBP2-34215.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0015.jpg)
![Immunohistochemistry-Paraffin: Synaptotagmin 1 Antibody [NBP2-34215] - Staining of human pancreas using Anti-SYT1 antibody NBP2-34215.](http://images.novusbio.com/fullsize/Synaptotagmin-1-Antibody-Immunohistochemistry-Paraffin-NBP2-34215-img0016.jpg)
![Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of Rat brain, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-2B2F10-Western-Blot-NBP3-15706-img0004.jpg "Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of Rat brain, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of various cell lines, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-2B2F10-Western-Blot-NBP3-15706-img0005.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Immunohistochemistry of paraffin-embedded mouse brain using Syntaxin 1A Rabbit mAb (NBP3-15706) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-2B2F10-Immunohistochemistry-Paraffin-NBP3-15706-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Syntaxin 1A Antibody (2B2F10) [Syntaxin 1A] - Immunofluorescence analysis of paraffin-embedded rat brain using Syntaxin 1A Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15706_rabbit-syntaxin-1a-mab-2b2f10-522025173456.jpg)
![Immunocytochemistry/ Immunofluorescence: Syntaxin 1A Antibody (2B2F10) [Syntaxin 1A] - Immunofluorescence analysis of paraffin-embedded mouse brain using Syntaxin 1A Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15706_rabbit-syntaxin-1a-mab-2b2f10-522025173352.jpg)
![Western Blot: VGLUT1 Antibody (S28-9) [NBP2-59329] - Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (NBP2-59329). Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (NBP2-59329) at 1:1000.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-S28-9-Western-Blot-NBP2-59329-img0005.jpg "Western Blot: VGLUT1 Antibody (S28-9) [NBP2-59329] - Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT1 protein using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (NBP2-59329). Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (NBP2-59329) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: VGLUT1 Antibody (S28-9) [NBP2-59329] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (NBP2-59329). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (NBP2-59329) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-S28-9-Immunocytochemistry-Immunofluorescence-NBP2-59329-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: VGLUT1 Antibody (S28-9) [NBP2-59329] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT1 Monoclonal Antibody, Clone S28-9 (NBP2-59329). Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-VGLUT1 Monoclonal Antibody (NBP2-59329) at 1:100. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) VGLUT1 Antibody. (D) Hoechst (E) Composite.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-S28-9-Immunocytochemistry-Immunofluorescence-NBP2-59329-img0007.jpg)
![Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining in human cerebral cortex and skeletal muscle tissues . Corresponding SYT7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SYT7-Antibody-Immunohistochemistry-Paraffin-NBP1-81588-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining in human cerebral cortex and skeletal muscle tissues . Corresponding SYT7 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: SYT7 Antibody [NBP1-81588] - Analysis in control (vector only transfected HEK293T lysate) and SYT7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/SYT7-Antibody-Western-Blot-NBP1-81588-img0015.jpg)
![Western Blot: SYT7 Antibody [NBP1-81588] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/SYT7-Antibody-Western-Blot-NBP1-81588-img0009.jpg)
![Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/SYT7-Antibody-Immunohistochemistry-Paraffin-NBP1-81588-img0010.jpg)
![Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/SYT7-Antibody-Immunohistochemistry-Paraffin-NBP1-81588-img0011.jpg)
![Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining of human prostate shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SYT7-Antibody-Immunohistochemistry-Paraffin-NBP1-81588-img0012.jpg)
![Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/SYT7-Antibody-Immunohistochemistry-Paraffin-NBP1-81588-img0013.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249383145.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-212024943559.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Drosophila tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249403847.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249371547.jpg)
![Western Blot: Synaptotagmin 1 Antibody (HL1654) - Azide and BSA Free [NBP3-25706] - Human tissue extract (5 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-2120249415950.jpg)
![Synaptotagmin 1 antibody [HL1654] detects Synaptotagmin 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Synaptotagmin 1 stained by Synaptotagmin 1 antibody [HL1654] (NBP3-25706) diluted at 1:250.Red: beta Tubulin 3/ Tuj1, a neural marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25706_rabbit-synaptotagmin-1-mab-hl1654-azide-and-bsa-free-immunohistochemistry-paraffin-14112025183373.jpg)
![Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4A expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Western-Blot-H00006810-M04-img0011.jpg "Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4A expression in NIH/3T3 (Cat # L018V1).")
![Immunocytochemistry/Immunofluorescence: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of monoclonal antibody to STX4A on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Immunocytochemistry-Immunofluorescence-H00006810-M04-img0007.jpg)
![Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4A expression in HeLa (Cat # L013V1).](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Western-Blot-H00006810-M04-img0009.jpg)
![Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4A expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Western-Blot-H00006810-M04-img0010.jpg)
![Western Blot: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Analysis of STX4 expression in transfected 293T cell line by STX4A monoclonal antibody (M04), clone 6D1. Lane 1: STX4 transfected lysate (Predicted MW: 34.2 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Western-Blot-H00006810-M04-img0012.jpg)
![Sandwich ELISA: Syntaxin 4 Antibody (6D1) [H00006810-M04] - Detection limit for recombinant GST tagged STX4A is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Syntaxin-4-Antibody-6D1-Sandwich-ELISA-H00006810-M04-img0008.jpg)
![Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (NBP3-25742) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-2120249393566.jpg "Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (NBP3-25742) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Frozen: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - VGAT antibody [HL1615] detects VGAT protein at cell membrane by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: VGAT stained by VGAT antibody [HL1615] (NBP3-25742) diluted at 1:100. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-212024941475.jpg)
![Immunohistochemistry-Paraffin: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - VGAT antibody [HL1615] detects VGAT protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. VGAT stained by VGAT antibody [HL1615] (NBP3-25742) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-2120249415931.jpg)
![Western Blot: VIAAT/SLC32A1/VGAT Antibody (HL1615) - Azide and BSA Free [NBP3-25742] - Boiled and unboiled mouse tissue extract (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (NBP3-25742) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25742_rabbit-viaat-slc32a1-vgat-mab-hl1615-azide-and-bsa-free-212024943286.jpg)
![Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-288202412493458.jpg "Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32961) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-298202414161869.jpg)
![Immunocytochemistry/ Immunofluorescence: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunocytochemistry analysis of SH-SY5Y cells labeling SNAP25 with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202410484855.jpg)
![Flow Cytometry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Flow cytometric analysis of SH-SY5Y cells labeling SNAP25. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32961, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202416372172.jpg)
![Western Blot: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of SNAP23 expression in transfected 293T cell line by SNAP23 monoclonal antibody (M01), clone 2F5-3D4.Lane 1: SNAP23 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Western-Blot-H00008773-M01-img0009.jpg "Western Blot: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of SNAP23 expression in transfected 293T cell line by SNAP23 monoclonal antibody (M01), clone 2F5-3D4.Lane 1: SNAP23 transfected lysate (Predicted MW: 23.4 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of monoclonal antibody to SNAP23 on formalin-fixed paraffin-embedded human spleen. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Immunohistochemistry-Paraffin-H00008773-M01-img0006.jpg)
![Western Blot: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - SNAP23 expression in Raw 264.7.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Western-Blot-H00008773-M01-img0001.jpg)
![Immunoprecipitation: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Analysis of SNAP23 transfected lysate using anti-SNAP23 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SNAP23 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Immunoprecipitation-H00008773-M01-img0007.jpg)
![Sandwich ELISA: SNAP23 Antibody (2F5-3D4) [H00008773-M01] - Detection limit for recombinant GST tagged SNAP23 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/SNAP23-Antibody-2F5-3D4-Sandwich-ELISA-H00008773-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-39000] - Analysis in human cerebral cortex and pancreas tissues. Corresponding SLC17A7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-39000-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-39000] - Analysis in human cerebral cortex and pancreas tissues. Corresponding SLC17A7 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-39000] - Staining of human cerebellum, cerebral cortex, pancreas and testis using Anti-VGLUT1 antibody NBP2-39000 (A) shows similar protein distribution across tissues to independent antibody NBP2-33597 (B).](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-39000-img0011.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-39000] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-39000-img0013.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-39000] - Staining of human cerebral cortex shows positivity in neuropil.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-39000-img0003.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-39000] - Staining of human cerebellum shows moderate cytoplasmic positivity in cells in granular layer.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-39000-img0008.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-39000] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-39000-img0010.jpg)
![Immunohistochemistry-Paraffin: VGLUT1 Antibody [NBP2-39000] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/VGLUT1-Antibody-Immunohistochemistry-Paraffin-NBP2-39000-img0012.jpg)
![Western Blot: SNAP23 Antibody [NBP1-83162] - Analysis in human cell line HepG2.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Western-Blot-NBP1-83162-img0006.jpg "Western Blot: SNAP23 Antibody [NBP1-83162] - Analysis in human cell line HepG2.")
![Immunocytochemistry/Immunofluorescence: SNAP23 Antibody [NBP1-83162] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83162-img0008.jpg)
![Immunohistochemistry-Paraffin: SNAP23 Antibody [NBP1-83162] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/SNAP23-Antibody-Immunohistochemistry-Paraffin-NBP1-83162-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Piccolo Antibody [NBP1-90250] - Staining of human cell line U-251 MG shows localization to nuclear speckles & plasma membrane. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Piccolo-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90250-img0004.jpg "Immunocytochemistry/Immunofluorescence: Piccolo Antibody [NBP1-90250] - Staining of human cell line U-251 MG shows localization to nuclear speckles & plasma membrane. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Piccolo Antibody [NBP1-90250] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/Piccolo-Antibody-Immunohistochemistry-Paraffin-NBP1-90250-img0006.jpg)
![Immunohistochemistry-Paraffin: Piccolo Antibody [NBP1-90250] - Staining of human testis shows weak cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Piccolo-Antibody-Immunohistochemistry-Paraffin-NBP1-90250-img0008.jpg)
