| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90116-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21835-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54679-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93743-0.02ml | |||
![Western Blot: CHIP/STUB1 Antibody (034) [NBP2-90116] - Anti-CHIP/STUB1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HELA Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Western-Blot-NBP2-90116-img0005.jpg "Western Blot: CHIP/STUB1 Antibody (034) [NBP2-90116] - Anti-CHIP/STUB1 rabbit monoclonal antibody at 1:500 dilution. Lane A: HELA Whole Cell Lysate. Lane B: 293T Whole Cell Lysate. Lane C: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: CHIP/STUB1 Antibody (034) [NBP2-90116] - Flow cytometric analysis of Human STUB1 expression on Hela cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human STUB1, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Flow-Cytometry-NBP2-90116-img0004.jpg)
![Immunoprecipitation: CHIP/STUB1 Antibody (034) [NBP2-90116] - STUB1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-STUB1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-STUB1 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-034-Immunoprecipitation-NBP2-90116-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CHIP/STUB1 Antibody (034) [NBP2-90116] - Immunofluorescence staining of STUB1 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-90116 antibody (dilution ratio 1:300) overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm and Nucleus.](http://images.novusbio.com/fullsize/nbp2-90116_rabbit-monoclonal-chip-stub1-antibody-034-235202514331566.png)
![Western Blot: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33072) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-28820241419150.jpg "Western Blot: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Western blot analysis of UBA3 on different lysates with Mouse anti-UBA3 antibody (NBP3-33072) at 1/500 dilution. Lane 1: HepG2 cell lysate Lane 2: Hela cell lysate Lane 3: 293T cell lysate Lane 4: K-562 cell lysate Lane 5: RAW264.7 cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 52 kDa Observed band size: 52 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33072) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-UBA3 antibody (NBP3-33072) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33072) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-29820241420944.jpg)
![Immunocytochemistry/ Immunofluorescence: UBA3 Antibody (1B5-2-3-R) [NBP3-33072] - Immunocytochemistry analysis of SiHa cells labeling UBA3 with Mouse anti-UBA3 antibody (NBP3-33072) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Mouse anti-UBA3 antibody (NBP3-33072) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33072_mouse-uba3-mab-1b5-2-3-r-29202410334093.jpg)
![Western Blot: RBX1 Antibody [NBP2-20113] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/RBX1-Antibody-Western-Blot-NBP2-20113-img0001.jpg "Western Blot: RBX1 Antibody [NBP2-20113] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 15% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: RBX1 Antibody [NBP2-20113] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with RBX1 antibody (NBP2-20113) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20113_rabbit-polyclonal-rbx1-antibody-910202420104483.jpg)
![Western Blot: CHIP/STUB1 Antibody [NB100-77315] - Detection of Human and Mouse STUB1/CHIP on HeLa whole cell lysate using NB100-77315. STUB1/CHIP was also immunoprecipitated by rabbit anti-STUB1/CHIP antibody NB100-77316.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-Western-Blot-NB100-77315-img0006.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: CHIP/STUB1 Antibody [NB100-77315] - Detection of Human and Mouse STUB1/CHIP on HeLa whole cell lysate using NB100-77315. STUB1/CHIP was also immunoprecipitated by rabbit anti-STUB1/CHIP antibody NB100-77316.")
![Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - Lane 1.: 293T cell lysate Lane 2.: A431 cell lysate Lane 3.: K562 cell lysate Lane 4.: NIH3T3 cell lysate](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-AT4B9-Western-Blot-NBP2-50579-img0003.jpg "Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - Lane 1.: 293T cell lysate Lane 2.: A431 cell lysate Lane 3.: K562 cell lysate Lane 4.: NIH3T3 cell lysate")
![Immunocytochemistry/Immunofluorescence: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - Analysis of 14-3-3 gamma in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human 14-3-3 gamma antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-AT4B9-Immunocytochemistry-Immunofluorescence-NBP2-50579-img0004.jpg)
![Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - The lysates of Hela (40ug) and Mouse brain (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000) Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-AT4B9-Western-Blot-NBP2-50579-img0001.jpg)
![Western Blot: 14-3-3 gamma Antibody (AT4B9) [NBP2-50579] - The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PDVF membrane and probed with anti-human 14-3-3 gamma antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. Recombinant Human YWHAZ Lane 2. Recombinant Human YWHAB Lane 3. Recombinant Human YWHAE Lane 4. Recombinant Human YWHAH Lane 5.: Recombinant Human YWHAG Lane 6.: Recombinant Human SFN Lane 7.: Recombinant Human YWHAQ.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-AT4B9-Western-Blot-NBP2-50579-img0002.jpg)
![Western Blot: RBX1 Antibody (SR1116) [NBP3-21835] - Western blot analysis using NBP3-21835 in MCF7 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21835_rabbit-rbx1-mab-sr1116-57202316112173.jpg "Western Blot: RBX1 Antibody (SR1116) [NBP3-21835] - Western blot analysis using NBP3-21835 in MCF7 cell lysate.")
![Western Blot: CHIP/STUB1 Antibody [H00010273-B01P] - Analysis of STUB1 expression in NIH/3T3.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-Western-Blot-H00010273-B01P-img0003.jpg "Western Blot: CHIP/STUB1 Antibody [H00010273-B01P] - Analysis of STUB1 expression in NIH/3T3.")
![Western Blot: CHIP/STUB1 Antibody [H00010273-B01P] - Analysis of STUB1 expression in PC-12.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-Western-Blot-H00010273-B01P-img0001.jpg)
![Western Blot: CHIP/STUB1 Antibody [H00010273-B01P] - Analysis of STUB1 expression in HepG2.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-Western-Blot-H00010273-B01P-img0002.jpg)
![Western Blot: CHIP/STUB1 Antibody [H00010273-B01P] - Analysis of STUB1 expression in transfected 293T cell line by STUB1 polyclonal antibody. Lane 1: STUB1 transfected lysate(33.33 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CHIP-STUB1-Antibody-Western-Blot-H00010273-B01P-img0004.jpg)
![SDS-Page: RBX1 Recombinant Protein [H00009978-P01]](http://images.novusbio.com/fullsize/RBX1-Recombinant-Protein-SDS-Page-H00009978-P01-img0001.jpg "SDS-Page: RBX1 Recombinant Protein [H00009978-P01]")
![Western Blot: 14-3-3 gamma Antibody [NBP2-54679] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-Western-Blot-NBP2-54679-img0003.jpg "Western Blot: 14-3-3 gamma Antibody [NBP2-54679] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4")
![Immunohistochemistry-Paraffin: 14-3-3 gamma Antibody [NBP2-54679] - Staining of human cerebral cortex shows cytoplasmic and nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-Immunohistochemistry-Paraffin-NBP2-54679-img0005.jpg)
![Western Blot: 14-3-3 gamma Antibody [NBP2-54679] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)](http://images.novusbio.com/fullsize/14-3-3-gamma-Antibody-Western-Blot-NBP2-54679-img0002.jpg)
![Western Blot: UBL5 Antibody [NBP2-86885] - WB Suggested Anti-Ubl5 Antibody. Titration: 1.0 ug/ml. Positive Control: Rat Liver](http://images.novusbio.com/fullsize/UBL5-Antibody-Western-Blot-NBP2-86885-img0003.jpg "Western Blot: UBL5 Antibody [NBP2-86885] - WB Suggested Anti-Ubl5 Antibody. Titration: 1.0 ug/ml. Positive Control: Rat Liver")
![Immunohistochemistry-Paraffin: UBL5 Antibody [NBP2-86885] - Rabbit Anti-Ubl5 antibody. Formalin Fixed Paraffin Embedded Tissue: Human Testis. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20x.](http://images.novusbio.com/fullsize/UBL5-Antibody-Immunohistochemistry-Paraffin-NBP2-86885-img0002.jpg)
![Immunohistochemistry-Paraffin: UBL5 Antibody [NBP2-86885] - Rabbit Anti-Ubl5 antibody. Formalin Fixed Paraffin Embedded Tissue: Human Kidney. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20x.](http://images.novusbio.com/fullsize/UBL5-Antibody-Immunohistochemistry-Paraffin-NBP2-86885-img0001.jpg)
![Immunohistochemistry;RBX1 Antibody[NBP3-30392]Detection of human ROC1 by immunohistochemistry. Sample: FFPE section of human osteosarcoma. Antibody: Affinity purified rabbit anti-ROC1 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-30395_rabbit-rbx1-pab-78202410153536.jpg "Immunohistochemistry;RBX1 Antibody[NBP3-30392]Detection of human ROC1 by immunohistochemistry. Sample: FFPE section of human osteosarcoma. Antibody: Affinity purified rabbit anti-ROC1 used at a dilution of 1:1,000 . Detection: DAB")
![Immunohistochemistry;RBX1 Antibody[NBP3-30395]Detection of human ROC1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-ROC1 used at a dilution of 1:1,000 . Detection: DAB](http://images.novusbio.com/fullsize/nbp3-30395_rabbit-rbx1-pab-78202410153530.jpg)
![Western Blot: NDUFA2 Antibody [NBP2-93743] - Analysis of extracts of various cell lines, using NDUFA2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/NDUFA2-Antibody-Western-Blot-NBP2-93743-img0004.jpg "Western Blot: NDUFA2 Antibody [NBP2-93743] - Analysis of extracts of various cell lines, using NDUFA2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: NDUFA2 Antibody [NBP2-93743] - Analysis of NIH/3T3 cells using NDUFA2 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/NDUFA2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93743-img0001.jpg)