| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33074 | |||
| IHC-Prediluted | NBP2-44976 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56709SS | |||
| Azide and BSA Free | NBP2-80613 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80607 | |||
| BSA Free | NB100-1830SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-203SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-71656SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59770SS | |||
| Azide and BSA Free | NBP2-80894 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-204SS | |||
![Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Western Blot Analysis of human HeLa cell lysate using p53 Antibody (BP53-12).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Western-Blot-NBP2-29453-img0010.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - p53 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using a 1 ug/mL mouse anti-p53 monoclonal (NBP2-29453, Novus Biologicals) for 1 hour at room temperature followed by the appropriate HRP polymer reagent, anti-mouse IgG VisUCyte HRP polymer (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Immunohistochemistry-Paraffin-NBP2-29453-img0008.jpg)
![Western Blot: p53 Antibody (BP53-12) [NBP2-29453] - Analysis of p53 1) A431 2) MCF7 and 3) HEK293 lysate probed with p53 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Western-Blot-NBP2-29453-img0001.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (BP53-12) [NBP2-29453] - Formalin-paraffin human colon carcinoma stained with p53 Ab (BP53-12).](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Immunohistochemistry-Paraffin-NBP2-29453-img0003.jpg)
![Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Simple Western lane view shows a specific band for p53 in 0.2 mg/ml of MDA-MB-231 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Simple-Western-NBP2-29453-img0004.jpg)
![Simple Western: p53 Antibody (BP53-12) [NBP2-29453] - Electropherogram image of the corresponding Simple western lane view. p53 antibody was used at 10 ug/ml dilution on MDA-MB-231 lysate(s) respectively.](http://images.novusbio.com/fullsize/p53-Antibody-BP53-12-Simple-Western-NBP2-29453-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Methanol-fixed MEF cells](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser536]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82088-img0009.jpg "Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Methanol-fixed MEF cells")
![Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Paraffin-embedded human breast carcinoma tissue.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser536]-Antibody-Immunohistochemistry-Paraffin-NB100-82088-img0007.jpg)
![Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Paraffin-embedded human Lung carcinoma tissue](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser536]-Antibody-Immunohistochemistry-Paraffin-NB100-82088-img0008.jpg)
![Western Blot: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Western Blot analysis of various cells using NB100-82088 diluted at 1:2000](http://images.novusbio.com/fullsize/nb100-82088_rabbit-polyclonal-rela-nfkb-p65-p-ser536-antibody-23420241545352.jpg)
![Immunocytochemistry/ Immunofluorescence: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Immunofluorescence staining of methanol-fixed Hela cells using NB100-82088.](http://images.novusbio.com/fullsize/nb100-82088_rabbit-polyclonal-rela-nfkb-p65-p-ser536-antibody-103202523464512.jpg)
![Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser536] Antibody [NB100-82088] - Analysis of paraffin-embedded human breast carcinoma tissue using NB100-82088 incubated with blocking peptide (right)](http://images.novusbio.com/fullsize/nb100-82088_rabbit-polyclonal-rela-nfkb-p65-p-ser536-antibody-103202523464555.jpg)
![Western Blot: Caspase-3 Antibody (31A893) [NB100-56709] - Curcumin Analogs Induce Apoptosis in Cancerous Cells by Several Pathways. E6-1 cells were treated for 24 hours with or without the broad spectrum caspase inhibitor ZVAD(oMe)-FMK and the studied compounds, lysed and subjected to Western blot analysis. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-01230-4) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Western-Blot-NB100-56709-img0009.jpg "<b>Biological Strategies Validation. </b>Western Blot: Caspase-3 Antibody (31A893) [NB100-56709] - Curcumin Analogs Induce Apoptosis in Cancerous Cells by Several Pathways. E6-1 cells were treated for 24 hours with or without the broad spectrum caspase inhibitor ZVAD(oMe)-FMK and the studied compounds, lysed and subjected to Western blot analysis. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-017-01230-4) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A893) [NB100-56709] - Tissue section of normal human breast using 5 ug/mL concentration of . Very strong diffused as well as granular immunopositivity of Caspase 3 was observed specifically in the cytoplasmic of ductal /acinar epithelial cells.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Immunohistochemistry-Paraffin-NB100-56709-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Caspase-3 Antibody (31A893) [NB100-56709] - Left panel: Untreated Jurkat cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with at 10 ug/mL overnight at 4C and detected with an anti-Mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Immunocytochemistry-Immunofluorescence-NB100-56709-img0008.jpg)
![Western Blot: Caspase-3 Antibody (31A893) [NB100-56709] - Analysis for human Caspase-3 using HL60 lysates with Caspase-3 Antibody (31A893) at 2 ug/mL dilution. NB100-56709 only detects a 32 kD Caspase-3 corresponding to pro-Caspase-3.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Western-Blot-NB100-56709-img0005.jpg)
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: Caspase-3 Antibody (31A893) [NB100-56709] - Analysis of Caspase-3 in Jurkat cells. Cells were treated with 2 uM staurosporine for different time periods. Caspase-3 activation is detected in Western blots by the presence of Caspase-3 cleavage fragments. These antibodies detect both pro (full-length) and active (cleaved) Caspase-3, depending on the treatment time points. A basal level of endogenous active Caspase-3 may be see in untreated Jurkat cells.](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Western-Blot-NB100-56709-img0006.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A893) [NB100-56709] - Caspase-3 was detected in immersion fixed paraffin-embedded sections of human bladder tissue using 5 ug/mL of mouse monoclonal Caspase-3 Antibody (31A893) (NB100-56709, Novus Biologicals), for 1 hour at room temperature followed by anti-mouse IgG VisUCyte HRP polymer (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize/Caspase-3-Antibody-31A893-Immunohistochemistry-Paraffin-NB100-56709-img0007.jpg)
![Western Blot: Caspase-3 Antibody (31A893) - BSA Free [NB100-56709] - Lemongrass extract is dependent on the production of oxidative stress to induce apoptosis. (E) E6-1 cells treated for 3 hrs with LG with or without NAC, lysed, & subjected to SDS-PAGE. Cells then transferred to a PVDF membrane & probed for the specific proteins. Bands visualized with a chemiluminescence reagent. Statistical calculations performed using Two-Way ANOVA multiple comparison for (A) & One-Way ANOVA multiple comparison for (B–C). **p < 0.01 vs. Control, ****p < 0.0001 vs. Control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22502), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56709_mouse-monoclonal-caspase-3-antibody-31a893-31020241535657.jpg)
![Western Blot: Caspase-3 Antibody (31A893) - BSA Free [NB100-56709] - Functioning FADD protein is required to induce apoptosis in cancer cells treated with lemongrass extract(A) U-937 & (B) DN FADD Jurkat cells were treated for 12 hours & 48 hours, respectively, with the specified treatments, lysed, & subjected to SDS-PAGE. Cells were then transferred to a PVDF membrane & probed for the specific proteins. Bands were visualized with a chemiluminescence reagent. (C) DN FADD Jurkat cells were treated for 48 hours with the specified doses & stained with Annexin V & PI. Results were obtained using image-based cytometry with the Y-axis representative of percent of cells positive for Annexin V (green), PI (red), Annexin V & PI (yellow), or negative for both Annexin V & PI (blue). Values are expressed as a mean ± SD from three independent experiments. (D) DN FADD Jurkat cells were treated with H2DCFDA following treatments with paraquat (PQ), LG, or WT for 3 hours. Results were obtained using the image-based cytometry with the Y-axis representative of percent of cells positive for DCF. Values are expressed as a mean ± SD from three independent experiments. Statistical calculations were performed using Two-Way ANOVA multiple comparison for (C) & One-Way ANOVA multiple comparison for (D). ***p < 0.001 vs. Control, ****p < 0.0001 vs. Control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22502), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56709_mouse-monoclonal-caspase-3-antibody-31a893-310202415525230.jpg)
![Western Blot: Caspase-3 Antibody (31A893) - BSA Free [NB100-56709] - Functioning FADD protein is required to induce apoptosis in cancer cells treated with lemongrass extract(A) U-937 & (B) DN FADD Jurkat cells were treated for 12 hours & 48 hours, respectively, with the specified treatments, lysed, & subjected to SDS-PAGE. Cells were then transferred to a PVDF membrane & probed for the specific proteins. Bands were visualized with a chemiluminescence reagent. (C) DN FADD Jurkat cells were treated for 48 hours with the specified doses & stained with Annexin V & PI. Results were obtained using image-based cytometry with the Y-axis representative of percent of cells positive for Annexin V (green), PI (red), Annexin V & PI (yellow), or negative for both Annexin V & PI (blue). Values are expressed as a mean ± SD from three independent experiments. (D) DN FADD Jurkat cells were treated with H2DCFDA following treatments with paraquat (PQ), LG, or WT for 3 hours. Results were obtained using the image-based cytometry with the Y-axis representative of percent of cells positive for DCF. Values are expressed as a mean ± SD from three independent experiments. Statistical calculations were performed using Two-Way ANOVA multiple comparison for (C) & One-Way ANOVA multiple comparison for (D). ***p < 0.001 vs. Control, ****p < 0.0001 vs. Control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.22502), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-56709_mouse-monoclonal-caspase-3-antibody-31a893-310202415535124.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0012.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.")
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Analysis of Calcium Sensing Receptor expression in rat kidney tissue.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0011.jpg)
![Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Image shows a specific band for Calcium Sensing Receptor in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Simple-Western-NB100-1830-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241003248.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on Hek293 cells with Calcium-sensing R/CaSR Antibody (HL 1499) NB100-1830PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-1772024959417.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Analysis of FFPE human brain using Calcium-sensing R/CaSR antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxid](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241044639.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on HEK293 cells with Calcium-sensing R/CaSR Antibody [HL 1499] NB100-1830F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-177202495941.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using calcium sensing receptor antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720249362617.jpg)
![Western Blot: HIP1 Antibody (4B10) [NB300-203] - Western Blot on HeLa whole cell extracts.](http://images.novusbio.com/fullsize/HIP1-Antibody-4B10-Western-Blot-NB300-203-img0003.jpg "Western Blot: HIP1 Antibody (4B10) [NB300-203] - Western Blot on HeLa whole cell extracts.")
![Western Blot: RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - Inhibits TNF-induced NF-kB activation: KBM-5 cells were incubated with various concentrations of peptides for 1 hr and treated with 0.1nM TNF for 30min. Nuclear extracts were prepared and NF-kB activation was analyzed by EMSA (Please refer toTakada et al, 2004 for further details). PTD-p65-P6 suppresses TNF-induced NF-kB activation by 25% at 100 uM and completely at 150 uM, where as the control peptide, PTD or p65-P6 without PTD did not have any effect.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529--p-Ser536]-Inhibitor-Peptide-Set-Western-Blot-NBP2-29321-img0015.jpg "Western Blot: RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - Inhibits TNF-induced NF-kB activation: KBM-5 cells were incubated with various concentrations of peptides for 1 hr and treated with 0.1nM TNF for 30min. Nuclear extracts were prepared and NF-kB activation was analyzed by EMSA (Please refer toTakada et al, 2004 for further details). PTD-p65-P6 suppresses TNF-induced NF-kB activation by 25% at 100 uM and completely at 150 uM, where as the control peptide, PTD or p65-P6 without PTD did not have any effect.")
![Western Blot: RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - Effect of various p65-inhibitor peptides on binding of purified recombinant p65 protein to the DNA in vitro. Recombinant p65 protein (100 ng/sample) was incubated for 30 minutes with 50uM of various peptides in 0.025 ml and then assayed for DNA binding activity by EMSA (Please refer to Takada et al, 2004 (3) for further details).](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser529--p-Ser536]-Inhibitor-Peptide-Set-Western-Blot-NBP2-29321-img0013.jpg)
![Functional (Inhibition): RelA/NFkB p65 [p Ser529, p Ser536] Inhibitor Peptide Set [NBP2-29321] - TLR5/NF-kB/SEAPorter HEK 293 (NBP2-26277) cells were plated in 96-well plates at 5 x 10^4 cells/well for 16 h. Cells were preincubated with different concentrations (0, 1, 5, 10, 25 and 50 uM) of Inhibitory Peptide (NBP2-29321) and Control Peptide (NBP2-29334) for 1 h. Cells were then stimulated with 1 ng/ml Flagellin (NBP2-25289) for 24 h. Secreted alkaline phosphatase (SEAP) was analyzed using SEAPorter Assay Kit (NBP2-25285). *p < 0.05 versus control peptide at the corresponding concentrations (Mann-Whitney U test).](http://images.novusbio.com/fullsize/RelA-NFkB-p65-p-Ser529-p-Ser536-Inhibitor-Peptide-Set-Functional-Inhibition-NBP2-29321-img0012.jpg)
![Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Western-Blot-NBP1-71656-img0009.jpg "Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.")
![Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1E8-1D9) [NBP1-71656] - OPA1 antibody was tested in ARPE-19 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Immunocytochemistry-Immunofluorescence-NBP1-71656-img0007.jpg)
![Immunohistochemistry: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 on mouse skin using NBP1-71656.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Immunohistochemistry-NBP1-71656-img0008.jpg)
![Simple Western: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Image shows a specific band for OPA1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-Simple-Western-NBP1-71656-img0010.jpg)
![Simple Western: OPA1 Antibody (1E8-1D9) - BSA Free [NBP1-71656] - Image shows a specific band for OPA1 in 0.1 ug/uL mouse hippocampus tissue lysate. Primary antibody dilution: 1:200. Image from verified customer review.](http://images.novusbio.com/fullsize/OPA1-Antibody-1E8-1D9-BSA-Free-Simple-Western-NBP1-71656-img0011.jpg)
![Western Blot: OPA1 Antibody (1284B) [NBP2-59770] - Total protein from Mouse Neuro2A cells, Human HeLa cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-OPA1 in blocking buffer, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Western-Blot-NBP2-59770-img0001.jpg "Western Blot: OPA1 Antibody (1284B) [NBP2-59770] - Total protein from Mouse Neuro2A cells, Human HeLa cells and Rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-OPA1 in blocking buffer, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1284B) [NBP2-59770] - A431 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with OPA1 Antibody [1284B] conjugated to Biotin (NBP2-59770B) at 5 ug/ml for 1 hour at room temperature then detected with Streptavidin conjugated to DyLight 550. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Immunocytochemistry-Immunofluorescence-NBP2-59770-img0022.jpg)
![Immunohistochemistry-Paraffin: OPA1 Antibody (1284B) [NBP2-59770] - IHC analysis of a formalin fixed paraffin embedded (FFPE) tissue section of mouse testes using OPA1 antibody at 1:1000 dilution. The primary antibody bound to OPA1 antigens in the tissue section was deteced using an HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This OPA1 antibody generated a diffused cytoplasmic staining of OPA1 protein in the tubular epithelial cells, the spermatids/spermatocytes and Leydig's cells.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Immunohistochemistry-Paraffin-NBP2-59770-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1284B) [NBP2-59770] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-OPA1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/OPA1-Antibody-1284B-Immunocytochemistry-Immunofluorescence-NBP2-59770-img0002.jpg)
![Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using NB100-82086](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunohistochemistry-Paraffin-NB100-82086-img0009.jpg "Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using NB100-82086")
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Staining of methanol-fixed Hela cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunocytochemistry-Immunofluorescence-NB100-82086-img0014.jpg)
![Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Human breast carcinoma tissue.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunohistochemistry-Paraffin-NB100-82086-img0010.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Western Blot analysis of various cells using NB100-82086 diluted at 1:1000](http://images.novusbio.com/fullsize/nb100-82086_rabbit-polyclonal-rela-nfkb-p65-p-ser276-antibody-23420241545358.jpg)
![Immunohistochemistry-Paraffin: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Analysis of paraffin- embedded human breast carcinoma tissue using NB100-82086 incubated with blocking peptide (right)](http://images.novusbio.com/fullsize/nb100-82086_rabbit-polyclonal-rela-nfkb-p65-p-ser276-antibody-103202523464543.jpg)
![Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with TNF using NB100-82086](http://images.novusbio.com/fullsize/nb100-82086_rabbit-polyclonal-rela-nfkb-p65-p-ser276-antibody-11320251555922.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NB100-82086] - Lane 1: HEK293. Lane 2: Lentivirus mediated NFkB overexpression. We image submitted by a verified customer review.](http://images.novusbio.com/fullsize/antibody/nb100-82086_rabbit-polyclonal-rela-nfkb-p65-p-ser276-antibody-western-blot-1132025204015..jpg)
![Western Blot: DRP1 Antibody (3B5) [H00010059-M01] - DNM1L monoclonal antibody (M01), clone 3B5 Analysis of DNM1L expression in K-562.](http://images.novusbio.com/fullsize/DRP1-Antibody-3B5-Western-Blot-H00010059-M01-img0008.jpg "Western Blot: DRP1 Antibody (3B5) [H00010059-M01] - DNM1L monoclonal antibody (M01), clone 3B5 Analysis of DNM1L expression in K-562.")
![Immunohistochemistry-Paraffin: DRP1 Antibody (3B5) [H00010059-M01] - Analysis of monoclonal antibody to DNM1L on formalin-fixed paraffin-embedded human leiomyosarcoma tissue. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/DRP1-Antibody-3B5-Immunohistochemistry-Paraffin-H00010059-M01-img0006.jpg)
![Western Blot: DRP1 Antibody (3B5) [H00010059-M01] - Analysis of DNM1L expression in transfected 293T cell line by DNM1L monoclonal antibody (M01), clone 3B5.Lane 1: DNM1L transfected lysate(79 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/DRP1-Antibody-3B5-Western-Blot-H00010059-M01-img0009.jpg)
![ELISA: DRP1 Antibody (3B5) [H00010059-M01] - Detection limit for recombinant GST tagged DNM1L is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/DRP1-Antibody-3B5-Sandwich-ELISA-H00010059-M01-img0007.jpg)
![Western Blot: HIP1 Antibody (1B11) [NB300-204] - HeLa whole cell extracts.](http://images.novusbio.com/fullsize/HIP1-Antibody-1B11-Western-Blot-NB300-204-img0002.jpg "Western Blot: HIP1 Antibody (1B11) [NB300-204] - HeLa whole cell extracts.")
![Immunocytochemistry/Immunofluorescence: HIP1 Antibody (1B11) [NB300-204] - Human pluripotent embryonal carcinoma cells stained with HIP1 antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/HIP1-Antibody-1B11-Immunocytochemistry-Immunofluorescence-NB300-204-img0003.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - PG-4 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NBP2-15336-img0021.jpg "Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - PG-4 whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Analysis of methanol-fixed A549, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15336-img0001.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Detects ALDH1A1 protein by Western blot analysis. A. 50 ug rat liver lysate/extract 10 % SDS-PAGE Dilution: 1:5000](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Western-Blot-NBP2-15336-img0012.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Detects ALDH1A1 protein by Western blot analysis. A. 50 ug mouse liver lysate/extract 10 % SDS-PAGE Dilution: 1:5000](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Western-Blot-NBP2-15336-img0015.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH1A1 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-Western-Blot-NBP2-15336-img0018.jpg)
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody [NBP2-15336] - Human gastric cancer, using ALDH1A1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/ALDH1A1-Antibody-Immunohistochemistry-Paraffin-NBP2-15336-img0002.jpg)
