| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74053 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50538-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83196-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-83197-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-93596SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70166 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92639-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59322-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-59632 | |||
![Western Blot: SELENBP1 Antibody (3H1) [NBP2-45979] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SELENBP1.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-3H1-Western-Blot-NBP2-45979-img0008.jpg "Western Blot: SELENBP1 Antibody (3H1) [NBP2-45979] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SELENBP1.")
![Immunohistochemistry: SELENBP1 Antibody (3H1) [NBP2-45979] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SELENBP1-Antibody-3H1-Immunohistochemistry-NBP2-45979-img0007.jpg)
![Immunohistochemistry: SELENBP1 Antibody (3H1) [NBP2-45979] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SELENBP1-Antibody-3H1-Immunohistochemistry-NBP2-45979-img0001.jpg)
![Immunohistochemistry: SELENBP1 Antibody (3H1) [NBP2-45979] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SELENBP1-Antibody-3H1-Immunohistochemistry-NBP2-45979-img0002.jpg)
![Immunohistochemistry: SELENBP1 Antibody (3H1) [NBP2-45979] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SELENBP1-Antibody-3H1-Immunohistochemistry-NBP2-45979-img0003.jpg)
![Immunohistochemistry: SELENBP1 Antibody (3H1) [NBP2-45979] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SELENBP1-Antibody-3H1-Immunohistochemistry-NBP2-45979-img0004.jpg)
![Immunohistochemistry: SELENBP1 Antibody (3H1) [NBP2-45979] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SELENBP1-Antibody-3H1-Immunohistochemistry-NBP2-45979-img0005.jpg)
![Immunohistochemistry: SELENBP1 Antibody (3H1) [NBP2-45979] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SELENBP1-Antibody-3H1-Immunohistochemistry-NBP2-45979-img0006.jpg)
![Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/p53-Antibody-Pab-DO-1-Western-Blot-NBP2-50538-img0001.jpg "Western Blot: p53 Antibody (Pab DO-1) [NBP2-50538] - B) Western Blot analyses of RPMI-8402 and NALM-6 cell lines treated for 24 h with AZD-1775 (185 nM) and PF-00477736 (25 and 250 nM respectively). B-actin was used for loading normalization. For relative quantification of each protein see Figure S3A and for whole western blot images see Figure S6. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/11/11/1654) licensed under a CC-BY license.")
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ reduces HIF-1 alpha through different mechanisms in MCF-7 & MDA-MB-231. (A) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. After 24 hours, cells were treated with DCQ (5 μM) for 6 hours under hypoxia. Whole cell lysates were prepared, & blots were probed against indicated antibodies. (B) MCF-7 cells were transfected with siRNA against p53 & ctrl siRNA (scrambled sequence) using lipofectamine 2000. Transfected cells were treated with DCQ (5 μM) for 6 hours under hypoxia. The extent of DNA fragmentation was determined by TUNEL assay 24 hours later using flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (C) Whole cell lysates were prepared after pretreating MCF-7 & MDA-MB-231 with the proteasome inhibitor MG132 (3 μM) then treated with DCQ, & blots were probed for HIF-1 alpha . Results are from three independent experiments. (D) Whole cell lysates of MCF-7 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-AKT, mTOR, p-mTOR & GAPDH. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-310202416114753.jpg)
![Western Blot: p53 Antibody (Pab DO-1) - BSA Free [NBP2-50538] - DCQ induces DNA damage & apoptosis via reactive oxygen species. (A) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for p-H2AX, p53, p-p53, p21, HIF-1 alpha & GAPDH. Results are representative of three independent experiments. (B) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 25 min incubation with 10 μM CM-H2DCFDA dye. Cells were washed with PBS & treated with DCQ for 1 hour under normoxia or hypoxia, after which cells were harvested & the amount of DCF fluorescence was analyzed by flow cytometry. Each percentage is the average ± SE of three independent experiments. (C) MDA-MB-231 & MCF-7 cells were pretreated with 1 mM Vitamin E or DTT for 2 hours followed by 6 hours treatment with DCQ under normoxia or hypoxia. After 24 hours, the extent of DNA fragmentation was determined by TUNEL assay & measured by flow cytometry. One-way ANOVA was used to compare DCQ-treated versus control & statistical significance of p < 0.05 is indicated by *. (D) Whole cell lysates of MCF-7 & MDA-MB-231 were prepared after 6 hours of exposure to DCQ (5 μM) under normoxia or hypoxia, & blots were probed for HIF-1 alpha & GAPDH. Results are representative of three independent experiments. Image collected & cropped by CiteAb from the following publication (//molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-13-12), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-31020241612246.jpg)
![Flow Cytometry: Mouse Monoclonal p53 Antibody (Pab DO-1) [NBP2-50538] - P53 expression in monocyte subset of Human PBMCs post IFN gamma treatment. Image from a verified customer review. The customer used the Alexa Fluor® 647-conjugated version of NBP2-50538, which is listed under NBP2-50538AF647.](http://images.novusbio.com/fullsize/antibody/nbp2-50538_mouse-p53-mab-pab-do-1-bsa-free-flow-cytometry-152025141858..png)
![Western Blot: SELENBP1 Antibody (OTI3H1) - Azide and BSA Free [NBP2-74053] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SELENBP1.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-OTI3H1-Azide-and-BSA-Free-Western-Blot-NBP2-74053-img0001.jpg "Western Blot: SELENBP1 Antibody (OTI3H1) - Azide and BSA Free [NBP2-74053] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SELENBP1.")
![Immunohistochemistry: SELENBP1 Antibody (OTI3H1) - Azide and BSA Free [NBP2-74053] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SELENBP1-Antibody-OTI3H1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74053-img0007.jpg)
![Immunohistochemistry: SELENBP1 Antibody (OTI3H1) - Azide and BSA Free [NBP2-74053] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SELENBP1-Antibody-OTI3H1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74053-img0002.jpg)
![Immunohistochemistry: SELENBP1 Antibody (OTI3H1) - Azide and BSA Free [NBP2-74053] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SELENBP1-Antibody-OTI3H1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74053-img0003.jpg)
![Immunohistochemistry: SELENBP1 Antibody (OTI3H1) - Azide and BSA Free [NBP2-74053] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SELENBP1-Antibody-OTI3H1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74053-img0004.jpg)
![Immunohistochemistry: SELENBP1 Antibody (OTI3H1) - Azide and BSA Free [NBP2-74053] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SELENBP1-Antibody-OTI3H1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74053-img0005.jpg)
![Immunohistochemistry: SELENBP1 Antibody (OTI3H1) - Azide and BSA Free [NBP2-74053] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SELENBP1-Antibody-OTI3H1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74053-img0006.jpg)
![Immunohistochemistry: SELENBP1 Antibody (OTI3H1) - Azide and BSA Free [NBP2-74053] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SELENBP1-Antibody-OTI3H1-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74053-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: SELENBP1 Antibody [NBP1-83196] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoli fibrillar center.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-83196-img0005.jpg "Immunocytochemistry/Immunofluorescence: SELENBP1 Antibody [NBP1-83196] - Immunofluorescent staining of human cell line HeLa shows localization to nucleoli fibrillar center.")
![Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83196] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83196-img0017.jpg)
![Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83196] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83196-img0006.jpg)
![Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83196] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83196-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83196] - Staining in human liver and pancreas tissues using anti-SELENBP1 antibody. Corresponding SELENBP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83196-img0008.jpg)
![Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83196] - Staining of human fallopian tube.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83196-img0010.jpg)
![Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83196] - Staining of human colon.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83196-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83196] - Analysis in human colon and tonsil tissues. Corresponding SELENBP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83196-img0012.jpg)
![Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83196] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83196-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83196] - Staining of human colon, fallopian tube, liver and tonsil using Anti-SELENBP1 antibody NBP1-83196 (A) shows similar protein distribution across tissues to independent antibody NBP1-83197 (B).](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83196-img0015.jpg)
![Immunohistochemistry: SELENBP1 Antibody [NBP1-83196] - Staining of human Fallopian tube shows weak to moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-NBP1-83196-img0016.jpg)
![Western Blot: SELENBP1 Antibody [NBP1-83197] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Western-Blot-NBP1-83197-img0018.jpg "Western Blot: SELENBP1 Antibody [NBP1-83197] - Analysis in human cell line SK-BR-3.")
![Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83197] - Staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83197-img0017.jpg)
![Western Blot: SELENBP1 Antibody [NBP1-83197] - Analysis in control (vector only transfected HEK293T lysate) and SELENBP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Western-Blot-NBP1-83197-img0008.jpg)
![Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83197] - Staining of human liver shows high expression.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83197-img0005.jpg)
![Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83197] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83197-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83197] - Staining in human liver and pancreas tissues using anti-SELENBP1 antibody. Corresponding SELENBP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83197-img0007.jpg)
![Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83197] - Staining of human fallopian tube.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83197-img0010.jpg)
![Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83197] - Staining of human colon.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83197-img0011.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83197] - Analysis in human colon and tonsil tissues. Corresponding SELENBP1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83197-img0012.jpg)
![Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83197] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83197-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-83197] - Staining of human colon, fallopian tube, liver and tonsil using Anti-SELENBP1 antibody NBP1-83197 (A) shows similar protein distribution across tissues to independent antibody NBP1-83196 (B).](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-83197-img0015.jpg)
![Immunohistochemistry: SELENBP1 Antibody [NBP1-83197] - Staining of human Fallopian tube shows weak to moderate nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-NBP1-83197-img0016.jpg)
![Western Blot: SELENBP1 Antibody [NBP1-54805] - Antibody Titration: 1 ug/ml Human heart.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Western-Blot-NBP1-54805-img0006.jpg "Western Blot: SELENBP1 Antibody [NBP1-54805] - Antibody Titration: 1 ug/ml Human heart.")
![Immunohistochemistry: SELENBP1 Antibody [NBP1-54805] - Human Adult heart Observed Staining: Cytoplasmic,Membrane (weak) Primary Antibody Concentration: 1 : 600 Secondary Antibody: Donkey anti-Rabbit-Cy2/3 Secondary Antibody Concentration: 1 : 200 Magnification: 20X Exposure Time: 0.5 2.0 sec.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-NBP1-54805-img0005.jpg)
![Western Blot: SELENBP1 Antibody [NBP1-54805] - Titration: 0.2-1 ug/ml, Positive Control: SH-SYSY cell lysate.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Western-Blot-NBP1-54805-img0003.jpg)
![Western Blot: SELENBP1 Antibody [NBP1-54805] - Antibody Titration: 1 ug/ml Human liver.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Western-Blot-NBP1-54805-img0007.jpg)
![Immunohistochemistry-Paraffin: SELENBP1 Antibody [NBP1-54805] - SELENBP1 in colon mucosa (Mouse), dilution 1:500, 10x, 20x and 40x. Image from verfied customer review.](http://images.novusbio.com/fullsize/SELENBP1-Antibody-Immunohistochemistry-Paraffin-NBP1-54805-img0004.jpg)
![Western Blot: SIAH1/2 Antibody (8G7H12) [NB110-93596] - Anti-SINA/SIAH monoclonal antibodies recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila. SINA monoclonal antibody 8G7H12 is highly speci](http://images.novusbio.com/fullsize/SIAH1-2-Antibody-8G7H12-Western-Blot-NB110-93596-img0002.jpg "Western Blot: SIAH1/2 Antibody (8G7H12) [NB110-93596] - Anti-SINA/SIAH monoclonal antibodies recognize both Drosophila SINA and human SIAH. There are two SINA-like E3 ligases, SINA and SINAH, in Drosophila. SINA monoclonal antibody 8G7H12 is highly speci")
![Western Blot: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiALOX5.(1:2000)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Western-Blot-NBP2-46513-img0003.jpg "Western Blot: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiALOX5.(1:2000)")
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Adenocarcinoma of Human ovary tissue using antiALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0014.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human colon tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0005.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human Kidney tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,(1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0007.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Carcinoma of Human kidney tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min (1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0010.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human liver tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0015.jpg)
![Western Blot: CYTB Antibody [NBP2-92639] - Analysis of extracts of various cell lines, using CYTB at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/CYTB-Antibody-Western-Blot-NBP2-92639-img0004.jpg "Western Blot: CYTB Antibody [NBP2-92639] - Analysis of extracts of various cell lines, using CYTB at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: CYTB Antibody [NBP2-92639] - Analysis of L929 cells using CYTB . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CYTB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92639-img0001.jpg)
![Immunohistochemistry: CYTB Antibody - Azide and BSA Free [NBP2-92639] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using CYTB Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-92639_rabbit-polyclonal-cytb-antibody-4220251430441.jpg)
![Immunohistochemistry: CYTB Antibody - Azide and BSA Free [NBP2-92639] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using CYTB Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-92639_rabbit-polyclonal-cytb-antibody-422025142903.jpg)
![Immunohistochemistry: CYTB Antibody - Azide and BSA Free [NBP2-92639] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using CYTB Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-92639_rabbit-polyclonal-cytb-antibody-42202514251882.jpg)
![Western Blot: CACNA1G Antibody (S178A-9) [NBP2-59322] - Western Blot analysis of Rat brain membrane lysate showing detection of CACNA1G Calcium Channel protein using Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Primary Antibody: Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody (NBP2-59322) at 1:1000.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-S178A-9-Western-Blot-NBP2-59322-img0005.jpg "Western Blot: CACNA1G Antibody (S178A-9) [NBP2-59322] - Western Blot analysis of Rat brain membrane lysate showing detection of CACNA1G Calcium Channel protein using Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Primary Antibody: Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody (NBP2-59322) at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CACNA1G Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-CACNA1G Monoclonal Antibody (NBP2-59322) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) CACNA1G Antibody (D) Composite.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-S178A-9-Immunocytochemistry-Immunofluorescence-NBP2-59322-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav3.1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-S178A-9-Immunocytochemistry-Immunofluorescence-NBP2-59322-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CACNA1G Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CACNA1G Monoclonal Antibody (NBP2-59322) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) CACNA1G Antibody. (D) Composite.](http://images.novusbio.com/fullsize/CACNA1G-Antibody-S178A-9-Immunocytochemistry-Immunofluorescence-NBP2-59322-img0007.jpg)
![Western Blot: Mouse Monoclonal CACNA1G Antibody (S178A-9) [NBP2-59322] - Western blot using lysates from human primary astrocytes or IGR39 melanoma cell line. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-59322_mouse-monoclonal-cacna1g-antibody-s178a-9-western-blot-1122023151422..png)
![Western Blot: p53 Antibody (TP53/1799R) [NBP2-59631] - Western blot analysis of HeLa cell lysate using p53 antibody (TP53/1799R).](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Western-Blot-NBP2-59631-img0005.jpg "Western Blot: p53 Antibody (TP53/1799R) [NBP2-59631] - Western blot analysis of HeLa cell lysate using p53 antibody (TP53/1799R).")
![Immunohistochemistry-Paraffin: p53 Antibody (TP53/1799R) [NBP2-59631] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained withp53 Recombinant Rabbit Monoclonal Antibody (TP53/1799R)](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Immunohistochemistry-Paraffin-NBP2-59631-img0002.jpg)
![Flow Cytometry: p53 Antibody (TP53/1799R) [NBP2-59631] - Flow Cytometric Analysis of HeLa cells using p53 Recombinant Rabbit Monoclonal Antibody (TP53/1799R) followed by goat anti-rabbit IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Flow-Cytometry-NBP2-59631-img0004.jpg)
![Western Blot: p53 Antibody (TP53/1799R) [NBP2-59631] - Western Blot Analysis of HeLa cell lysate using p53 Antibody (TP53/1799R).](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Western-Blot-NBP2-59631-img0003.jpg)
![Protein Array: p53 Antibody (TP53/1799R) [NBP2-59631] - containing more than 19,000 full-length human proteins. Z- and S- Score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary) produces when binding to a particular protein on the HuProt array. Z-scores are described in units of standard deviations above the mean value of all signals generated on that array. If targets on HuProt are arranged in descending order of the Z-score, the S-score is the difference between the Z-score. S-score therefore represents the relative target specificity of an Ab to its intended target. An Ab is considered to specific to its intended target, if the Ab has an S-score of at least 2.5. For example, if Ab binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Ab to protein X is equal to 29](http://images.novusbio.com/fullsize/p53-Antibody-TP53-1799R-Protein-Array-NBP2-59631-img0001.jpg)
![Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/p53-Antibody-Western-Blot-NBP2-19667-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: p53 Antibody [NBP2-19667] - p53 antibody [N1], N-term detects p53 protein at nucleus by immunofluorescent analysis.Samples: HCT 116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p53 protein stained by p53 antibody [N1], N-term.Blue: Hoechst 33342 staining.Scale bar = 10 um.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: p53 Antibody [NBP2-19667] - Confocal immunofluorescence analysis of paraformaldehyde-fixed U2OS, using p53 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/p53-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-19667-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: p53 Antibody [NBP2-19667] - Immunoprecipitation of p53 protein. HCT116 lysates with 30uM cisplatin treatment for 24 hours were subjected to immunoprecipitation using (B) normal rabbit IgG or (C) 2. 5 ug of anti-p53 antibody. (A) Input, 20ug of HCT116 lysates. The precipitated protein was detected by NBP2-19667. anti-Rabbit IgG Kit was used in the Western blot.](http://images.novusbio.com/fullsize/p53-Antibody-Immunoprecipitation-NBP2-19667-img0007.jpg)
![Whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody [N1], N-term (NBP2-19667) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-19667_rabbit-polyclonal-p53-antibody-western-blot-2611202518354313.jpg)