| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75924SS | |||
| Azide and BSA Free | NBP2-80828 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41287-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35905-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61121-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78393SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78392SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45340 | |||
| Azide and BSA Free | NBP2-34807-0.1mg | |||
![Western Blot: LC3A Antibody (2312D) [NBP2-75924] - Total protein from human HeLa and mouse Neuro2A cells treated with and without 50 uM chloroquine for 24 hours was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LC3A in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/LC3A-Antibody-2312D-Western-Blot-NBP2-75924-img0003.jpg "Western Blot: LC3A Antibody (2312D) [NBP2-75924] - Total protein from human HeLa and mouse Neuro2A cells treated with and without 50 uM chloroquine for 24 hours was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LC3A in blocking buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.")
![Immunohistochemistry-Paraffin: LC3A Antibody (2312D) [NBP2-75924] - This LC3A antibody was detected in immersion fixed paraffin-embedded sections of human brain (frontal cortex) using Rabbit Anti-Human LC3A Monoclonal Antibody (Catalog # NBP2-75924) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei.](http://images.novusbio.com/fullsize/LC3A-Antibody-2312D-Immunohistochemistry-Paraffin-NBP2-75924-img0004.jpg)
![Western Blot: Parkin Antibody [NBP2-41287] - Analysis of PARK2 in human cerebellum tissue lysate with PARK2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/Parkin-Antibody-Western-Blot-NBP2-41287-img0001.jpg "Western Blot: Parkin Antibody [NBP2-41287] - Analysis of PARK2 in human cerebellum tissue lysate with PARK2 antibody at 1 ug/ml.")
![Immunohistochemistry: Parkin Antibody - BSA Free [NBP2-41287] - Immunohistochemistry of Parkin in human brain tissue with Parkin antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41287_rabbit-polyclonal-parkin-antibody-271120241932611.jpg)
![Immunocytochemistry/ Immunofluorescence: Parkin Antibody - BSA Free [NBP2-41287] - Immunofluorescence of Parkin in human brain tissue with Parkin antibody at 20 u/ml.](http://images.novusbio.com/fullsize/nbp2-41287_rabbit-polyclonal-parkin-antibody-2711202420405583.jpg)
![Immunocytochemistry/ Immunofluorescence: Parkin Antibody - BSA Free [NBP2-41287] - Immunofluorescence of Parkin in human brain tissue with Parkin antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41287_rabbit-polyclonal-parkin-antibody-271120242074619.jpg)
![Western Blot: GATE-16/GABARAPL2 Antibody [NBP2-82090] - Analysis of GATE-16/GABARAPL2 in human brain tissue lysate with GATE-16/GABARAPL2 antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/GATE-16-GABARAPL2-Antibody-Western-Blot-NBP2-82090-img0001.jpg "Western Blot: GATE-16/GABARAPL2 Antibody [NBP2-82090] - Analysis of GATE-16/GABARAPL2 in human brain tissue lysate with GATE-16/GABARAPL2 antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: GATE-16/GABARAPL2 Antibody - BSA Free [NBP2-82090] - Immunofluorescence of GATE-16/GABARAPL2 in rat brain tissue with GATE-16/GABARAPL2 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82090_rabbit-polyclonal-gate-16-gabarapl2-antibody-271120242092042.jpg)
![Immunocytochemistry/ Immunofluorescence: GATE-16/GABARAPL2 Antibody - BSA Free [NBP2-82090] - Immunofluorescence of GATE-16/GABARAPL2 in mouse brain tissue with GATE-16/GABARAPL2 Antibody at 20 ug/mL.Green: GATE-16/GABARAPL2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-82090_rabbit-polyclonal-gate-16-gabarapl2-antibody-2711202420175113.gif)
![Immunohistochemistry: GATE-16/GABARAPL2 Antibody - BSA Free [NBP2-82090] - Immunohistochemistry of GATE-16/GABARAPL2 in rat brain tissue with GATE-16/GABARAPL2 antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82090_rabbit-polyclonal-gate-16-gabarapl2-antibody-271120241958411.jpg)
![Immunohistochemistry: GATE-16/GABARAPL2 Antibody - BSA Free [NBP2-82090] - Immunohistochemistry of GATE-16/GABARAPL2 in mouse brain tissue with GATE-16/GABARAPL2 Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-82090_rabbit-polyclonal-gate-16-gabarapl2-antibody-271120241928845.gif)
![Immunohistochemistry: PSMB5 Antibody [NBP3-35905] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using PSMB5 Rabbit pAb at a dilution of 1:600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35905_rabbit-psmb5-pab-912202414163039.jpg "Immunohistochemistry: PSMB5 Antibody [NBP3-35905] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using PSMB5 Rabbit pAb at a dilution of 1:600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Western Blot: PSMB5 Antibody [NBP3-35905] - Western blot analysis of various lysates, using PSMB5 Rabbit pAb at 1:6000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35905_rabbit-psmb5-pab-912202414112888.jpg)
![Immunohistochemistry: PSMB5 Antibody [NBP3-35905] - Immunohistochemistry analysis of PSMB5 in paraffin-embedded human breast tissue using PSMB5 Rabbit pAb at a dilution of 1:600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35905_rabbit-psmb5-pab-31120251704710.jpg%3Ft=1709604395)
![Immunohistochemistry: PSMB5 Antibody [NBP3-35905] - Immunohistochemistry analysis of PSMB5 in paraffin-embedded mouse Intestine tissue using PSMB5 Rabbit pAb at a dilution of 1:600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-35905_rabbit-psmb5-pab-3112025173130.jpg%3Ft=1709604395)
![Western Blot: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis using DNM1L mouse mAb against PC-12 (1), LNCAP (2) and NIH/3T3 (3) cell lysate.](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-Western-Blot-NBP2-37309-img0004.jpg "Western Blot: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis using DNM1L mouse mAb against PC-12 (1), LNCAP (2) and NIH/3T3 (3) cell lysate.")
![Immunocytochemistry/Immunofluorescence: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis of Hela cells using DNM1L mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-Immunocytochemistry-Immunofluorescence-NBP2-37309-img0005.jpg)
![Flow Cytometry: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis of HEK293 cells using DNM1L mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-Flow-Cytometry-NBP2-37309-img0006.jpg)
![Western Blot: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis using DNM1L mAb against human DNM1L recombinant protein. (Expected MW is 41.8 kDa)](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-Western-Blot-NBP2-37309-img0002.jpg)
![Western Blot: DRP1 Antibody (4A4C7) [NBP2-37309] - Analysis using DNM1L mAb against HEK293 (1) and DNM1L (AA: 69-213)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-Western-Blot-NBP2-37309-img0003.jpg)
![ELISA: DRP1 Antibody (4A4C7) [NBP2-37309] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/DRP1-Antibody-4A4C7-ELISA-NBP2-37309-img0001.jpg)
![Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Immunohistochemistry analysis of rat brain injected with Type 1 mouse alpha synuclein PFFs (NBP2-61596). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 mouse alpha synuclein PFFs (NBP2-61596) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Immunohistochemistry-NBP2-61596-img0003.jpg "Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Immunohistochemistry analysis of rat brain injected with Type 1 mouse alpha synuclein PFFs (NBP2-61596). Species: Female Sprague-Dawley Rat. Rat was injected with 16g Type 1 mouse alpha synuclein PFFs (NBP2-61596) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30 days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Primary antibody: rabbit monoclonal anti-pSer129 alpha synuclein. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the periform/insular cortex and the cingulate cortex on both the same (ipsi) and opposite (contra) sides as the injection sites.")
![In vitro assay: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - Active alpha synuclein aggregate seeds the formation of new alpha Synuclein aggregates from the pool of active monomers. Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha Synuclein aggregates. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha Synuclein protein aggregation) over time when 10 nM of active alpha Synuclein aggregate is combined with 100 uM of active alpha Synuclein monomer, as compared to active alpha Synuclein aggregate and active alpha Synuclein monomer alone. Thioflavin T excitation maximum = 450 nm; emission maximum = 485 nm.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-In-vitro-assay-NBP2-61596-img0002.jpg)
![Electron Microscopy: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TEM of Type 1 mouse alpha synuclein Pre-formed Fibrils (NBP2-61596). Fibrils were sonicated and image was taken at 100kx magnification.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-61596-img0005.jpg)
![Electron Microscopy: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TEM of Type 1 mouse alpha synuclein Pre-formed Fibrils (NBP2-61596). Image was taken at 100kx magnification.](http://images.novusbio.com/fullsize/Recombinant-Mouse-alpha-Synuclein-Active-Pre-formed-Fibrils-Type-1-Protein-Electron-Microscopy-NBP2-61596-img0004.jpg)
![Immunohistochemistry: Recombinant Mouse alpha-Synuclein Active, Pre-formed Fibrils, (Type 1) Protein [NBP2-61596] - TH fluorescence in mouse brain. Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-61596_recombinant-mouse-alpha-synuclein-active-pre-formed-fibrils-type-1-protein-immunohistochemistry-205202512497..jpg)
![Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Western-Blot-NBP2-61121-img0004.jpg "Western Blot: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Western blot analysis of Human, Mouse brain lysate showing detection of ~16 kDa alpha-Synuclein protein using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human brain lysate. Lane 3: Mouse brain lysate. Lane 4: Alpha Synuclein Monomer (0.5 ug). Load: 15 ug. Block: 2.5% BSA in TBST. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~16 kDa. Other Band(s): 100, 75, 45, 30,16 kDa.")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Phospho serine 129 antibody (NBP2-61121) was used to detect phosphorylated alpha synuclein in primary mouse hippocampal neurons treated with 100 nM sonicated mouse alpha synuclein PFFs (A). Phosphorylated alpha synuclein was visible in perinucleus and neurites compared to untreated control (B). Read the protocol at pabmabs.com/?p=7901. Image courtesy of Trine Rasmussen, Simon Molgaard Jensen at Aarhus University.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0006.jpg)
![Immunohistochemistry: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunohistochemistry analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Brain. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.](http://images.novusbio.com/fullsize/alpha-Synuclein-[p-Ser129]-Antibody-Immunohistochemistry-NBP2-61121-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein [p Ser129] Antibody [NBP2-61121] - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121). Tissue: Primary hippocampal neurons treated with active Alpha Synuclein Protein Aggregate (SPR-322) at 4 g/ml to induce fibrils. Species: Rat. Fixation: 4% paraformaldehyde. Primary Antibody: Rabbit Anti-alpha-Synuclein Polyclonal Antibody (NBP2-61121) at 1:500 for 24 hours at 4C. Secondary Antibody: Goat Anti-Rabbit Alexa Fluor 488 at 1:700 for 1 hour at RT. Counterstain: Guinea Pig Anti-NeuN (red) neuronal marker (Donkey Anti-Guinea Pig Alexa Fluor 647 1:700); Hoechst (blue) nuclear stain at 1:6000, 1:3000 for 60 min at RT, 5 min at RT. Magnification: 20X.](http://images.novusbio.com/fullsize/alpha-Synuclein [p Ser129] Antibody-Immunocytochemistry-Immunofluorescence-NBP2-61121-img0007.jpg)
![Western Blot: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-OTI1B8-Azide-and-BSA-Free-Western-Blot-NBP2-70854-img0001.jpg "Western Blot: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Glutathione Synthetase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti")
![Immunohistochemistry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - Staining of paraffin-embedded Human Kidney tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-OTI1B8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70854-img0004.jpg)
![Flow Cytometry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Glutathione Synthetase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-OTI1B8-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70854-img0002.jpg)
![Immunohistochemistry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-OTI1B8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70854-img0003.jpg)
![Immunohistochemistry: Glutathione Synthetase Antibody (OTI1B8) - Azide and BSA Free [NBP2-70854] - Staining of paraffin-embedded Human prostate tissue using anti-Glutathione Synthetase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Glutathione-Synthetase-Antibody-OTI1B8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70854-img0005.jpg)
![Western Blot: PRAS40 Antibody (JE30-60) [NBP3-32850] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 27 kDa Observed band size: 40 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32850) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-288202412442995.jpg "Western Blot: PRAS40 Antibody (JE30-60) [NBP3-32850] - Western blot analysis of PRAS40 on different lysates with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate Lysates/proteins at 40 ug/Lane. Predicted band size: 27 kDa Observed band size: 40 kDa Exposure time: 1 minute 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32850) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PRAS40 Antibody (JE30-60) [NBP3-32850] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32850) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-298202413585526.jpg)
![Immunocytochemistry/ Immunofluorescence: PRAS40 Antibody (JE30-60) [NBP3-32850] - Immunocytochemistry analysis of HeLa cells labeling PRAS40 with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PRAS40 antibody (NBP3-32850) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-2920241192362.jpg)
![Flow Cytometry: PRAS40 Antibody (JE30-60) [NBP3-32850] - Flow cytometric analysis of HeLa cells labeling PRAS40. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32850, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32850_rabbit-pras40-mab-je30-60-2920241637211.jpg)
![Immunocytochemistry/Immunofluorescence: SERF1A Antibody [NBP1-78393] - Serf1a antibody was tested in Ntera cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/SERF1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78393-img0005.jpg "Immunocytochemistry/Immunofluorescence: SERF1A Antibody [NBP1-78393] - Serf1a antibody was tested in Ntera cells with FITC (green). Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: SERF1A Antibody [NBP1-78393] - Analysis of SERF1A in human Alzheimer's brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/SERF1A-Antibody-Immunohistochemistry-NBP1-78393-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SERF1A Antibody [NBP1-78392] - Serf1a antibody was tested in Ntera2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/SERF1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-78392-img0003.jpg "Immunocytochemistry/Immunofluorescence: SERF1A Antibody [NBP1-78392] - Serf1a antibody was tested in Ntera2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).")
![Immunohistochemistry: SERF1A Antibody [NBP1-78392] - Analysis of SERF1A in human Alzheimer's brain using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/SERF1A-Antibody-Immunohistochemistry-NBP1-78392-img0004.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895] - Western Blot Analysis of human Brain lysate using UCH-L1/PGP9.5 Antibody (SPM574)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-SPM574-Western-Blot-NBP2-32895-img0005.jpg "Western Blot: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895] - Western Blot Analysis of human Brain lysate using UCH-L1/PGP9.5 Antibody (SPM574)")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895] - Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with UCH-L1/PGP9.5 Antibody (SPM574) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-SPM574-Immunocytochemistry-Immunofluorescence-NBP2-32895-img0004.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody (SPM574) [NBP2-32895] - Formalin-fixed. paraffin-embedded rat cerebellum stained with Pgp9.5/UchL1 Monoclonal antibody (SPM574)](http://images.novusbio.com/fullsize/UCH-L1-PGP9-5-Antibody-SPM574-Immunohistochemistry-Paraffin-NBP2-32895-img0003.jpg)